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live dead bacterial staining kit  (Beyotime)


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    Structured Review

    Beyotime live dead bacterial staining kit
    Live Dead Bacterial Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+kit/LIVE-DEAD+Bacterial+Staining+Kit+with+DMAO+%26+PI/pmc13059000-90-11-18
    Average 99 stars, based on 248 article reviews
    live dead bacterial staining kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Development of thymol-loaded biocompatible PLA/PLGA composite nanoparticles for oral health care application: Antimicrobial and antibiofilm activities
    Article Snippet: Biocompatible nanoformulations offer a promising approach to enhancing the bioavailability and bioactivity of phytochemicals such as thymol, thereby expanding their potential for oral healthcare applications.. This study fabricated thymol-loaded polylactic acid (PLA), poly (lactic-co-glycolic acid) (PLGA), and PLA-PLGA blend nanoparticles through single-emulsion solvent evaporation, and compared their physicochemical properties, antimicrobial activity, and preliminary biological safety comprehensively.. All nanoparticles exhibited a spherical and uniform morphology, while increasing the PLGA content resulted in a slightly more porous surface structure.

    Article Title: Arginine metabolism as a mutualistic strategy in the interaction between Porphyromonas gingivalis and Filifactor alocis.
    Article Snippet: .. For live/dead staining, suspensions adjusted to an OD600 of ~0.1 were diluted fivefold and stained with the DMAO/PI bacterial viability kit (Beyotime) according to the manufacturer’s instructions. .. After 30min, 10μL of stained suspension was applied to a glass slide and imaged using a fluorescence microscope (DM8, Leica, Germany).

    Article Title: Hybrid membrane-coated motors for atopic dermatitis therapy via pruritus relief and ILC2 suppression.
    Article Snippet: Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense pruritus, xerosis, and erythema.. Its pathogenesis involves hyperactivation of type 2 innate lymphoid cells (ILC2s) and excessive accumulation of interleukin-31(IL-31).. Moreover, over-colonization of Staphylococcus aureus exacerbates inflammation and skin barrier damage.

    Article Title: Characterization of a K62-specific phage depolymerase and its protective effect in a mouse model of Klebsiella pneumoniae infection.
    Article Snippet: .. Live/dead bacterial viability assay To evaluate the synergistic bactericidal effect of the depolymerase Dp3 and serum, a LIVE/DEAD Bacterial Staining Kit with DMAO & PI (Cat. No. C2030, Beyotime Biotechnology, China) was employed according to the manufacturer’s protocol. ..

    Article Title: Sonogenetic Control of Engineered Probiotics for the Treatment of MASH by Modulating the Gut-Liver Axis.
    Article Snippet: At each time point, samples were taken from both groups, and their optical density was determined with a Nanodrop 2000c spectrophotometer (Thermo Scientific) in cuvette mode. .. In accordance with the manufacturer’s protocol, the bacterial viability was determined using the Live/Dead Bacterial Staining Kit (DMAO/PI) (Beyotime, C2030S). ..

    Article Title: Biological and genomic characteristics of a polyvalent Salmonella phage and its dual application in food decontamination and chick salmonellosis treatment.
    Article Snippet: Bacteriophages have been widely recognized as one of the most promising alternatives to antibiotics.. In this study, a multivalent bacteriophage designated vB_SalM_Sz was isolated, and its whole-genome sequence, biological traits, biosafety profile, and bactericidal activity were comprehensively characterized.. Genomic and phenotypic analyses revealed that vB_SalM_Sz is a polyvalent phage capable of lysing both Salmonella spp. and Escherichia coli, taxonomically classified within the genus Rosemountvirus, class Caudoviricetes, family incertae sedis.

    Live Dead Assay:

    Article Title: Arginine metabolism as a mutualistic strategy in the interaction between Porphyromonas gingivalis and Filifactor alocis.
    Article Snippet: .. For live/dead staining, suspensions adjusted to an OD600 of ~0.1 were diluted fivefold and stained with the DMAO/PI bacterial viability kit (Beyotime) according to the manufacturer’s instructions. .. After 30min, 10μL of stained suspension was applied to a glass slide and imaged using a fluorescence microscope (DM8, Leica, Germany).

    Article Title: A mucoadhesive nanotherapeutic platform orchestrating acid control and redox homeostasis for enhanced gastric ulcer healing.
    Article Snippet: Conventional oral therapies for gastric ulcers are often limited by rapid gastric clearance and insufficient local drug accumulation, resulting in suboptimal therapeutic outcomes.. Here, we developed a dopamine-modified poly(β-amino ester) (DA-PBAE) mucoadhesive nanoplatform for the oral co-delivery of vonoprazan and edaravone (VE@DA-PBAE).. The amphiphilic DA-PBAE nanoparticles were constructed through a one-pot Michael addition reaction and integrated acid-triggered electrostatic interactions with catechol-mediated wet adhesion, enabling prolonged gastric mucosal retention and sustained release of dual therapeutic agents in the highly acidic gastric environment.

    CCK-8 Assay:

    Article Title: A mucoadhesive nanotherapeutic platform orchestrating acid control and redox homeostasis for enhanced gastric ulcer healing.
    Article Snippet: Conventional oral therapies for gastric ulcers are often limited by rapid gastric clearance and insufficient local drug accumulation, resulting in suboptimal therapeutic outcomes.. Here, we developed a dopamine-modified poly(β-amino ester) (DA-PBAE) mucoadhesive nanoplatform for the oral co-delivery of vonoprazan and edaravone (VE@DA-PBAE).. The amphiphilic DA-PBAE nanoparticles were constructed through a one-pot Michael addition reaction and integrated acid-triggered electrostatic interactions with catechol-mediated wet adhesion, enabling prolonged gastric mucosal retention and sustained release of dual therapeutic agents in the highly acidic gastric environment.

    H&E Stain:

    Article Title: A mucoadhesive nanotherapeutic platform orchestrating acid control and redox homeostasis for enhanced gastric ulcer healing.
    Article Snippet: Conventional oral therapies for gastric ulcers are often limited by rapid gastric clearance and insufficient local drug accumulation, resulting in suboptimal therapeutic outcomes.. Here, we developed a dopamine-modified poly(β-amino ester) (DA-PBAE) mucoadhesive nanoplatform for the oral co-delivery of vonoprazan and edaravone (VE@DA-PBAE).. The amphiphilic DA-PBAE nanoparticles were constructed through a one-pot Michael addition reaction and integrated acid-triggered electrostatic interactions with catechol-mediated wet adhesion, enabling prolonged gastric mucosal retention and sustained release of dual therapeutic agents in the highly acidic gastric environment.

    Masson's Trichrome Staining:

    Article Title: A mucoadhesive nanotherapeutic platform orchestrating acid control and redox homeostasis for enhanced gastric ulcer healing.
    Article Snippet: Conventional oral therapies for gastric ulcers are often limited by rapid gastric clearance and insufficient local drug accumulation, resulting in suboptimal therapeutic outcomes.. Here, we developed a dopamine-modified poly(β-amino ester) (DA-PBAE) mucoadhesive nanoplatform for the oral co-delivery of vonoprazan and edaravone (VE@DA-PBAE).. The amphiphilic DA-PBAE nanoparticles were constructed through a one-pot Michael addition reaction and integrated acid-triggered electrostatic interactions with catechol-mediated wet adhesion, enabling prolonged gastric mucosal retention and sustained release of dual therapeutic agents in the highly acidic gastric environment.

    Viability Assay:

    Article Title: Hybrid membrane-coated motors for atopic dermatitis therapy via pruritus relief and ILC2 suppression.
    Article Snippet: Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense pruritus, xerosis, and erythema.. Its pathogenesis involves hyperactivation of type 2 innate lymphoid cells (ILC2s) and excessive accumulation of interleukin-31(IL-31).. Moreover, over-colonization of Staphylococcus aureus exacerbates inflammation and skin barrier damage.

    Article Title: Characterization of a K62-specific phage depolymerase and its protective effect in a mouse model of Klebsiella pneumoniae infection.
    Article Snippet: .. Live/dead bacterial viability assay To evaluate the synergistic bactericidal effect of the depolymerase Dp3 and serum, a LIVE/DEAD Bacterial Staining Kit with DMAO & PI (Cat. No. C2030, Beyotime Biotechnology, China) was employed according to the manufacturer’s protocol. ..

    BIA-KA:

    Article Title: Process-resolved effects of dibutyl phthalate on sludge anaerobic fermentation: Enzyme inhibition and metabolic disruption underlie the suppression of acidogenesis.
    Article Snippet: Growing evidence suggests that leaching of plasticizers such as dibutyl phthalate (DBP) from microplastics inhibits methanogenesis in anaerobic digesters treating waste activated sludge.. However, how DBP influences upstream anaerobic fermentation (AF) remains unclear.. This study evaluated the effects of DBP on overall sludge AF and separately on solubilization, hydrolysis, and acidogenesis processes.

    Fluorescence:

    Article Title: Biological and genomic characteristics of a polyvalent Salmonella phage and its dual application in food decontamination and chick salmonellosis treatment.
    Article Snippet: Bacteriophages have been widely recognized as one of the most promising alternatives to antibiotics.. In this study, a multivalent bacteriophage designated vB_SalM_Sz was isolated, and its whole-genome sequence, biological traits, biosafety profile, and bactericidal activity were comprehensively characterized.. Genomic and phenotypic analyses revealed that vB_SalM_Sz is a polyvalent phage capable of lysing both Salmonella spp. and Escherichia coli, taxonomically classified within the genus Rosemountvirus, class Caudoviricetes, family incertae sedis.

    Microscopy:

    Article Title: Biological and genomic characteristics of a polyvalent Salmonella phage and its dual application in food decontamination and chick salmonellosis treatment.
    Article Snippet: Bacteriophages have been widely recognized as one of the most promising alternatives to antibiotics.. In this study, a multivalent bacteriophage designated vB_SalM_Sz was isolated, and its whole-genome sequence, biological traits, biosafety profile, and bactericidal activity were comprehensively characterized.. Genomic and phenotypic analyses revealed that vB_SalM_Sz is a polyvalent phage capable of lysing both Salmonella spp. and Escherichia coli, taxonomically classified within the genus Rosemountvirus, class Caudoviricetes, family incertae sedis.



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