pulp cell suspensions (SouthernBiotech)
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Pulp Cell Suspensions, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+buffer/Cell+Staining+Buffer/pmc11672625-115-0-39
Average 93 stars, based on 26 article reviews
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Staining:Article Title: Heavy chain antibodies binding to CD19 Article Snippet: Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with Article Title: Microdevice for differential separation, purification and amplification of forensic samples Article Snippet: .. When the samples were ready to be stained with the antibody, the eluate was spun down at 400×g for 5 min. and the supernatant was removed, which was followed by Article Title: Effects of Ikaros (IKZF1) gene in the virulence of Marek's disease virus. Article Snippet: Marek’s disease (MD) caused by the oncogenic avian herpesvirus, Marek’s disease virus (MDV) has significant economic impacts on the poultry industry because MDV is ubiquitous in the environment and most chickens are exposed to the threat by MDV from the first day of age.. Meq, a bZIP transactivator, is required for tumor formation by MDV, mostly T cell lymphomas.. Additionally, Ikaros (IKZF1) has been identified as a cancer driver gene for MDV tumorigenesis. Article Title: The structural basis of protective and nonprotective human monoclonal antibodies targeting the parainfluenza virus type 3 hemagglutinin-neuraminidase Article Snippet: Streptavidin-conjugated PE (Invitrogen, Cat #S866) and streptavidin-conjugated BV605 (BioLegend, Cat #405229) were slowly added to biotinylated HN at a fluorophore to protein molar ratio of 4:1 and stored on ice away from light. .. PBMCs (10 6 cells) were suspended in FACS buffer (PBS, 2% FBS, 2% goat serum, 0.5 mM EDTA) (1 mL) and Fc-blocked with Human TruStain FcX (BioLegend, Cat #422301) (5 μL/million cells) on ice for 30 min. A Article Title: The Bacterial Cell Wall Components Lipopolysaccharide and Peptidoglycan Initiate Divergent Local Tissue and Systemic Inflammatory Response Profiles in the Chicken Model Article Snippet: Briefly, the living tissue (pulp) was removed from the GF and then incubated in 0.1% collagenase/dispase (Collagenase type IV, Life Technologies, Carlsbad, CA, USA; Dispase II, Sigma-Aldrich, St. Louis, MO, USA) at 40 °C for 15 min and pushed through a 60 μm nylon mesh to prepare single-cell suspensions. .. Article Title: Feed additive carriers affected the physiological effects of a lactobacilli-based pro- and postbiotic in broilers Article Snippet: .. Eight μL of solution containing fixed Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM Purification:Article Title: Heavy chain antibodies binding to CD19 Article Snippet: Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with Incubation:Article Title: Heavy chain antibodies binding to CD19 Article Snippet: Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with Article Title: Effects of Ikaros (IKZF1) gene in the virulence of Marek's disease virus. Article Snippet: Marek’s disease (MD) caused by the oncogenic avian herpesvirus, Marek’s disease virus (MDV) has significant economic impacts on the poultry industry because MDV is ubiquitous in the environment and most chickens are exposed to the threat by MDV from the first day of age.. Meq, a bZIP transactivator, is required for tumor formation by MDV, mostly T cell lymphomas.. Additionally, Ikaros (IKZF1) has been identified as a cancer driver gene for MDV tumorigenesis. Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM Flow Cytometry:Article Title: Heavy chain antibodies binding to CD19 Article Snippet: Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with Article Title: Effects of Ikaros (IKZF1) gene in the virulence of Marek's disease virus. Article Snippet: Marek’s disease (MD) caused by the oncogenic avian herpesvirus, Marek’s disease virus (MDV) has significant economic impacts on the poultry industry because MDV is ubiquitous in the environment and most chickens are exposed to the threat by MDV from the first day of age.. Meq, a bZIP transactivator, is required for tumor formation by MDV, mostly T cell lymphomas.. Additionally, Ikaros (IKZF1) has been identified as a cancer driver gene for MDV tumorigenesis. Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM Labeling:Article Title: Effects of Ikaros (IKZF1) gene in the virulence of Marek's disease virus. Article Snippet: Marek’s disease (MD) caused by the oncogenic avian herpesvirus, Marek’s disease virus (MDV) has significant economic impacts on the poultry industry because MDV is ubiquitous in the environment and most chickens are exposed to the threat by MDV from the first day of age.. Meq, a bZIP transactivator, is required for tumor formation by MDV, mostly T cell lymphomas.. Additionally, Ikaros (IKZF1) has been identified as a cancer driver gene for MDV tumorigenesis. Article Title: The Bacterial Cell Wall Components Lipopolysaccharide and Peptidoglycan Initiate Divergent Local Tissue and Systemic Inflammatory Response Profiles in the Chicken Model Article Snippet: Briefly, the living tissue (pulp) was removed from the GF and then incubated in 0.1% collagenase/dispase (Collagenase type IV, Life Technologies, Carlsbad, CA, USA; Dispase II, Sigma-Aldrich, St. Louis, MO, USA) at 40 °C for 15 min and pushed through a 60 μm nylon mesh to prepare single-cell suspensions. .. FACS:Article Title: The structural basis of protective and nonprotective human monoclonal antibodies targeting the parainfluenza virus type 3 hemagglutinin-neuraminidase Article Snippet: Streptavidin-conjugated PE (Invitrogen, Cat #S866) and streptavidin-conjugated BV605 (BioLegend, Cat #405229) were slowly added to biotinylated HN at a fluorophore to protein molar ratio of 4:1 and stored on ice away from light. .. PBMCs (10 6 cells) were suspended in FACS buffer (PBS, 2% FBS, 2% goat serum, 0.5 mM EDTA) (1 mL) and Fc-blocked with Human TruStain FcX (BioLegend, Cat #422301) (5 μL/million cells) on ice for 30 min. A Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM Bioprocessing:Article Title: The Bacterial Cell Wall Components Lipopolysaccharide and Peptidoglycan Initiate Divergent Local Tissue and Systemic Inflammatory Response Profiles in the Chicken Model Article Snippet: Briefly, the living tissue (pulp) was removed from the GF and then incubated in 0.1% collagenase/dispase (Collagenase type IV, Life Technologies, Carlsbad, CA, USA; Dispase II, Sigma-Aldrich, St. Louis, MO, USA) at 40 °C for 15 min and pushed through a 60 μm nylon mesh to prepare single-cell suspensions. .. Expressing:Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM Transfection:Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM Cytometry:Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM |
