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pulp cell suspensions  (SouthernBiotech)


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    Structured Review

    SouthernBiotech pulp cell suspensions
    Pulp Cell Suspensions, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/Cell+Staining+Buffer/pmc11672625-115-0-39
    Average 93 stars, based on 26 article reviews
    pulp cell suspensions - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Heavy chain antibodies binding to CD19
    Article Snippet: Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with flow cytometry buffer (1X PBS, 1% BSA, 0.1% NaN3) and stained with goat F(ab′)2 anti-human IgG conjugated to R-phycoerythrin (PE) (Southern Biotech, cat. #2042-09) to detect cell-bound antibodies. ..

    Article Title: Microdevice for differential separation, purification and amplification of forensic samples
    Article Snippet: .. When the samples were ready to be stained with the antibody, the eluate was spun down at 400×g for 5 min. and the supernatant was removed, which was followed by resuspension in 300 μl of cell staining buffer (Southern Biotech, Birmingham, AL). ..

    Article Title: Effects of Ikaros (IKZF1) gene in the virulence of Marek's disease virus.
    Article Snippet: Marek’s disease (MD) caused by the oncogenic avian herpesvirus, Marek’s disease virus (MDV) has significant economic impacts on the poultry industry because MDV is ubiquitous in the environment and most chickens are exposed to the threat by MDV from the first day of age.. Meq, a bZIP transactivator, is required for tumor formation by MDV, mostly T cell lymphomas.. Additionally, Ikaros (IKZF1) has been identified as a cancer driver gene for MDV tumorigenesis.

    Article Title: The structural basis of protective and nonprotective human monoclonal antibodies targeting the parainfluenza virus type 3 hemagglutinin-neuraminidase
    Article Snippet: Streptavidin-conjugated PE (Invitrogen, Cat #S866) and streptavidin-conjugated BV605 (BioLegend, Cat #405229) were slowly added to biotinylated HN at a fluorophore to protein molar ratio of 4:1 and stored on ice away from light. .. PBMCs (10 6 cells) were suspended in FACS buffer (PBS, 2% FBS, 2% goat serum, 0.5 mM EDTA) (1 mL) and Fc-blocked with Human TruStain FcX (BioLegend, Cat #422301) (5 μL/million cells) on ice for 30 min. A staining mixture containing anti-human CD19-APC (BioLegend, Cat #302212), IgM-FITC (Southern Biotech, Cat #2020-02), IgD-FITC (Southern Biotech, Cat #2030-02), GhostDye Red 780 (Tonbo Biosciences, Cat #130865T100) or DAPI (ThermoFisher, Cat #62248) (live/dead stains), BT-SA conjugated PIV3 HN-PE and BT-SA conjugated PIV3 HN-BV605 was prepared and kept on ice away from light. ..

    Article Title: The Bacterial Cell Wall Components Lipopolysaccharide and Peptidoglycan Initiate Divergent Local Tissue and Systemic Inflammatory Response Profiles in the Chicken Model
    Article Snippet: Briefly, the living tissue (pulp) was removed from the GF and then incubated in 0.1% collagenase/dispase (Collagenase type IV, Life Technologies, Carlsbad, CA, USA; Dispase II, Sigma-Aldrich, St. Louis, MO, USA) at 40 °C for 15 min and pushed through a 60 μm nylon mesh to prepare single-cell suspensions. .. Pulp cell suspensions were washed and resuspended in PBS+ buffer (PBS, 1% bovine serum albumin and 0.1% sodium azide; VWR, Radnor, PA, USA) for immunofluorescent staining with a panel of fluorescently labeled (FITC, PE, or SPRD) mouse monoclonal antibodies (Southern Biotechnology Associates, Inc., Birmingham, AL, USA) using a three-color direct staining procedure. ..

    Article Title: Feed additive carriers affected the physiological effects of a lactobacilli-based pro- and postbiotic in broilers
    Article Snippet: .. Eight μL of solution containing fixed blood cells were stained in 100 μL buffer containing a combination of antibodies: anti-CD45-SPRD (clone LT40, 1 μg/mL), anti-CD4-PE-Cy7 (clone CT4, 0.05 μg/mL), anti-CD8α-AF700 (clone CT8, 0.1 μg/mL), anti-TCRγδ-AF488 (clone TCR1, 1 μg/mL), anti-Bu1-AF647 (clone AV20, 0.25 μg/mL), and Kul01-PE (0.2 μg/mL) (all from SouthernBiotech, Birmingham, USA), and anti-integrin αV/β3-AF405 (clone 23C6, 1 μg/mL, Santa Cruz Biotechnologies). .. After a 20 min incubation at 4 ◦C, 392 μL of BD CellFIX solution (BD Biosciences, Franklin Lakes, USA) were added before analysis on a MACSQuant 16 flow cytometer (Miltenyi, Paris, France) allowing analysis of fluorescence intensities and the absolute count of cells.

    Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto
    Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM EDTA (FACS buffer) Cells were then stained with mAbs H5.28, H5.31, or FI6v3 (10 μg/mL) for 30 min at RT and for 5 min at 37° C. The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4° C., fixed with 4% formaldehyde in PBS, and analyzed by flow cytometry using an LSR-2 cytometer (BD Biosciences). .. Data for a total of up to 20,000 cell events were acquired and flow cytometry data were analyzed with FlowJo software (Tree Star).

    Purification:

    Article Title: Heavy chain antibodies binding to CD19
    Article Snippet: Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with flow cytometry buffer (1X PBS, 1% BSA, 0.1% NaN3) and stained with goat F(ab′)2 anti-human IgG conjugated to R-phycoerythrin (PE) (Southern Biotech, cat. #2042-09) to detect cell-bound antibodies. ..

    Incubation:

    Article Title: Heavy chain antibodies binding to CD19
    Article Snippet: Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with flow cytometry buffer (1X PBS, 1% BSA, 0.1% NaN3) and stained with goat F(ab′)2 anti-human IgG conjugated to R-phycoerythrin (PE) (Southern Biotech, cat. #2042-09) to detect cell-bound antibodies. ..

    Article Title: Effects of Ikaros (IKZF1) gene in the virulence of Marek's disease virus.
    Article Snippet: Marek’s disease (MD) caused by the oncogenic avian herpesvirus, Marek’s disease virus (MDV) has significant economic impacts on the poultry industry because MDV is ubiquitous in the environment and most chickens are exposed to the threat by MDV from the first day of age.. Meq, a bZIP transactivator, is required for tumor formation by MDV, mostly T cell lymphomas.. Additionally, Ikaros (IKZF1) has been identified as a cancer driver gene for MDV tumorigenesis.

    Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto
    Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM EDTA (FACS buffer) Cells were then stained with mAbs H5.28, H5.31, or FI6v3 (10 μg/mL) for 30 min at RT and for 5 min at 37° C. The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4° C., fixed with 4% formaldehyde in PBS, and analyzed by flow cytometry using an LSR-2 cytometer (BD Biosciences). .. Data for a total of up to 20,000 cell events were acquired and flow cytometry data were analyzed with FlowJo software (Tree Star).

    Flow Cytometry:

    Article Title: Heavy chain antibodies binding to CD19
    Article Snippet: Materials and Methods CD19 Cell Binding Binding to CD19 positive cells was assessed by flow cytometry (Guava easyCyte 8HT, EMD Millipore) using the Daudi cell line (ATCC). .. Briefly, 100,000 target cells were stained with a dilution series of purified UniAbsTM for 30 minutes at 4° C. Following incubation, the cells were washed twice with flow cytometry buffer (1X PBS, 1% BSA, 0.1% NaN3) and stained with goat F(ab′)2 anti-human IgG conjugated to R-phycoerythrin (PE) (Southern Biotech, cat. #2042-09) to detect cell-bound antibodies. ..

    Article Title: Effects of Ikaros (IKZF1) gene in the virulence of Marek's disease virus.
    Article Snippet: Marek’s disease (MD) caused by the oncogenic avian herpesvirus, Marek’s disease virus (MDV) has significant economic impacts on the poultry industry because MDV is ubiquitous in the environment and most chickens are exposed to the threat by MDV from the first day of age.. Meq, a bZIP transactivator, is required for tumor formation by MDV, mostly T cell lymphomas.. Additionally, Ikaros (IKZF1) has been identified as a cancer driver gene for MDV tumorigenesis.

    Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto
    Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM EDTA (FACS buffer) Cells were then stained with mAbs H5.28, H5.31, or FI6v3 (10 μg/mL) for 30 min at RT and for 5 min at 37° C. The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4° C., fixed with 4% formaldehyde in PBS, and analyzed by flow cytometry using an LSR-2 cytometer (BD Biosciences). .. Data for a total of up to 20,000 cell events were acquired and flow cytometry data were analyzed with FlowJo software (Tree Star).

    Labeling:

    Article Title: Effects of Ikaros (IKZF1) gene in the virulence of Marek's disease virus.
    Article Snippet: Marek’s disease (MD) caused by the oncogenic avian herpesvirus, Marek’s disease virus (MDV) has significant economic impacts on the poultry industry because MDV is ubiquitous in the environment and most chickens are exposed to the threat by MDV from the first day of age.. Meq, a bZIP transactivator, is required for tumor formation by MDV, mostly T cell lymphomas.. Additionally, Ikaros (IKZF1) has been identified as a cancer driver gene for MDV tumorigenesis.

    Article Title: The Bacterial Cell Wall Components Lipopolysaccharide and Peptidoglycan Initiate Divergent Local Tissue and Systemic Inflammatory Response Profiles in the Chicken Model
    Article Snippet: Briefly, the living tissue (pulp) was removed from the GF and then incubated in 0.1% collagenase/dispase (Collagenase type IV, Life Technologies, Carlsbad, CA, USA; Dispase II, Sigma-Aldrich, St. Louis, MO, USA) at 40 °C for 15 min and pushed through a 60 μm nylon mesh to prepare single-cell suspensions. .. Pulp cell suspensions were washed and resuspended in PBS+ buffer (PBS, 1% bovine serum albumin and 0.1% sodium azide; VWR, Radnor, PA, USA) for immunofluorescent staining with a panel of fluorescently labeled (FITC, PE, or SPRD) mouse monoclonal antibodies (Southern Biotechnology Associates, Inc., Birmingham, AL, USA) using a three-color direct staining procedure. ..

    FACS:

    Article Title: The structural basis of protective and nonprotective human monoclonal antibodies targeting the parainfluenza virus type 3 hemagglutinin-neuraminidase
    Article Snippet: Streptavidin-conjugated PE (Invitrogen, Cat #S866) and streptavidin-conjugated BV605 (BioLegend, Cat #405229) were slowly added to biotinylated HN at a fluorophore to protein molar ratio of 4:1 and stored on ice away from light. .. PBMCs (10 6 cells) were suspended in FACS buffer (PBS, 2% FBS, 2% goat serum, 0.5 mM EDTA) (1 mL) and Fc-blocked with Human TruStain FcX (BioLegend, Cat #422301) (5 μL/million cells) on ice for 30 min. A staining mixture containing anti-human CD19-APC (BioLegend, Cat #302212), IgM-FITC (Southern Biotech, Cat #2020-02), IgD-FITC (Southern Biotech, Cat #2030-02), GhostDye Red 780 (Tonbo Biosciences, Cat #130865T100) or DAPI (ThermoFisher, Cat #62248) (live/dead stains), BT-SA conjugated PIV3 HN-PE and BT-SA conjugated PIV3 HN-BV605 was prepared and kept on ice away from light. ..

    Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto
    Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM EDTA (FACS buffer) Cells were then stained with mAbs H5.28, H5.31, or FI6v3 (10 μg/mL) for 30 min at RT and for 5 min at 37° C. The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4° C., fixed with 4% formaldehyde in PBS, and analyzed by flow cytometry using an LSR-2 cytometer (BD Biosciences). .. Data for a total of up to 20,000 cell events were acquired and flow cytometry data were analyzed with FlowJo software (Tree Star).

    Bioprocessing:

    Article Title: The Bacterial Cell Wall Components Lipopolysaccharide and Peptidoglycan Initiate Divergent Local Tissue and Systemic Inflammatory Response Profiles in the Chicken Model
    Article Snippet: Briefly, the living tissue (pulp) was removed from the GF and then incubated in 0.1% collagenase/dispase (Collagenase type IV, Life Technologies, Carlsbad, CA, USA; Dispase II, Sigma-Aldrich, St. Louis, MO, USA) at 40 °C for 15 min and pushed through a 60 μm nylon mesh to prepare single-cell suspensions. .. Pulp cell suspensions were washed and resuspended in PBS+ buffer (PBS, 1% bovine serum albumin and 0.1% sodium azide; VWR, Radnor, PA, USA) for immunofluorescent staining with a panel of fluorescently labeled (FITC, PE, or SPRD) mouse monoclonal antibodies (Southern Biotechnology Associates, Inc., Birmingham, AL, USA) using a three-color direct staining procedure. ..

    Expressing:

    Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto
    Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM EDTA (FACS buffer) Cells were then stained with mAbs H5.28, H5.31, or FI6v3 (10 μg/mL) for 30 min at RT and for 5 min at 37° C. The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4° C., fixed with 4% formaldehyde in PBS, and analyzed by flow cytometry using an LSR-2 cytometer (BD Biosciences). .. Data for a total of up to 20,000 cell events were acquired and flow cytometry data were analyzed with FlowJo software (Tree Star).

    Transfection:

    Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto
    Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM EDTA (FACS buffer) Cells were then stained with mAbs H5.28, H5.31, or FI6v3 (10 μg/mL) for 30 min at RT and for 5 min at 37° C. The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4° C., fixed with 4% formaldehyde in PBS, and analyzed by flow cytometry using an LSR-2 cytometer (BD Biosciences). .. Data for a total of up to 20,000 cell events were acquired and flow cytometry data were analyzed with FlowJo software (Tree Star).

    Cytometry:

    Article Title: Peptide vaccine based on a new universal influenza a hemagglutinin head domain epitope and human monoclonal antibodies binding thereto
    Article Snippet: .. HEK293F cells grown in expression medium were transfected transiently with cDNA encoding H3 A/Hong Kong/1/1968 HA protein and incubated at 37° C. for 36 h. Untransfected (UT) or transfected cells were washed and incubated with either DMEM containing TPCK trypsin (2 μg/mL) or plain DMEM for 15 min at 37° C. After incubation cells were washed with PBS containing 2% of heat-inactivated FBS and 2 mM EDTA (FACS buffer) Cells were then stained with mAbs H5.28, H5.31, or FI6v3 (10 μg/mL) for 30 min at RT and for 5 min at 37° C. The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4° C., fixed with 4% formaldehyde in PBS, and analyzed by flow cytometry using an LSR-2 cytometer (BD Biosciences). .. Data for a total of up to 20,000 cell events were acquired and flow cytometry data were analyzed with FlowJo software (Tree Star).



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    Image Search Results


    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

    Techniques: Flow Cytometry, Isolation, Staining