Review



transcription factor staining buffer  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Miltenyi Biotec transcription factor staining buffer
    Flow cytometry. A typical example of FACS based expression analysis for <t>transcription</t> factors (upper histograms) and surface receptors (lower histograms) of VSELs. Each histogram compares given antibodies (given in black numbers) and specifc isotype controls (given in red numbers). Percentage of positive VSELs and the median fluorescence were assessed
    Transcription Factor Staining Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/pmc09209346-166-8-14?v=Miltenyi+Biotec
    Average 97 stars, based on 51 article reviews
    transcription factor staining buffer - by Bioz Stars, 2026-08
    97/100 stars

    Images

    1) Product Images from "The Treasury of Wharton's Jelly"

    Article Title: The Treasury of Wharton's Jelly

    Journal: Stem Cell Reviews and Reports

    doi: 10.1007/s12015-021-10217-8

    Flow cytometry. A typical example of FACS based expression analysis for transcription factors (upper histograms) and surface receptors (lower histograms) of VSELs. Each histogram compares given antibodies (given in black numbers) and specifc isotype controls (given in red numbers). Percentage of positive VSELs and the median fluorescence were assessed
    Figure Legend Snippet: Flow cytometry. A typical example of FACS based expression analysis for transcription factors (upper histograms) and surface receptors (lower histograms) of VSELs. Each histogram compares given antibodies (given in black numbers) and specifc isotype controls (given in red numbers). Percentage of positive VSELs and the median fluorescence were assessed

    Techniques Used: Flow Cytometry, Expressing, Fluorescence

    Expression of pluripotency associated marker proteins examined by Immunofluorescence staining. VSELs were separated from UC-MSCs mass population, the tiny cells are between 5 and 7 μm and surrounded with dark spots ( a ). VSEL suspension cells were stained extracellular with anti-bodies against SSEA-4, CD184, and CD133 followed by nuclear staining of transcription factors Nanog, Oct-4, and Sox-2. Expression of each marker was detected. The nuclei were stained with HOECHST. Weak background Fluorescence signals of isotype controls were subtracted. The scale bars represented 50 μm ( b ). The magnification for SSEA-4/Sox-2 and CD133/Oct-4 was 20-fold and for CD184/Nanog 10-fold
    Figure Legend Snippet: Expression of pluripotency associated marker proteins examined by Immunofluorescence staining. VSELs were separated from UC-MSCs mass population, the tiny cells are between 5 and 7 μm and surrounded with dark spots ( a ). VSEL suspension cells were stained extracellular with anti-bodies against SSEA-4, CD184, and CD133 followed by nuclear staining of transcription factors Nanog, Oct-4, and Sox-2. Expression of each marker was detected. The nuclei were stained with HOECHST. Weak background Fluorescence signals of isotype controls were subtracted. The scale bars represented 50 μm ( b ). The magnification for SSEA-4/Sox-2 and CD133/Oct-4 was 20-fold and for CD184/Nanog 10-fold

    Techniques Used: Expressing, Marker, Immunofluorescence, Staining, Suspension, Fluorescence



    Similar Products

    transcription factor staining buffer  (Miltenyi Biotec)
    97
    Miltenyi Biotec transcription factor staining buffer
    Transcription Factor Staining Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/pmc13379542-331-6-12?v=Miltenyi+Biotec
    Average 97 stars, based on 1 article reviews
    transcription factor staining buffer - by Bioz Stars, 2026-08
    97/100 stars
    		  Buy from Supplier
    cells  (Miltenyi Biotec)
    97
    Miltenyi Biotec cells
    Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/pm42401537-343-3-9?v=Miltenyi+Biotec
    Average 97 stars, based on 1 article reviews
    cells - by Bioz Stars, 2026-08
    97/100 stars
    		  Buy from Supplier
    fox p3 buffer kit  (Miltenyi Biotec)
    97
    Miltenyi Biotec fox p3 buffer kit
    Fox P3 Buffer Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/bio_rxiv__64898__2026__06__30__735615-137-1-5?v=Miltenyi+Biotec
    Average 97 stars, based on 1 article reviews
    fox p3 buffer kit - by Bioz Stars, 2026-08
    97/100 stars
    		  Buy from Supplier
    foxp3 staining buffer  (Miltenyi Biotec)
    97
    Miltenyi Biotec foxp3 staining buffer
    Foxp3 Staining Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/pm42331382-367-13-19?v=Miltenyi+Biotec
    Average 97 stars, based on 1 article reviews
    foxp3 staining buffer - by Bioz Stars, 2026-08
    97/100 stars
    		  Buy from Supplier
    flow cytometry staining buffer  (Thermo Fisher)
    97
    Thermo Fisher flow cytometry staining buffer
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
    Flow Cytometry Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/pmc13068824-328-4-9?v=Thermo+Fisher
    Average 97 stars, based on 1 article reviews
    flow cytometry staining buffer - by Bioz Stars, 2026-08
    97/100 stars
    		  Buy from Supplier
    staining buffer  (Thermo Fisher)
    99
    Thermo Fisher staining buffer
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
    Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/bio_rxiv__64898__2026__06__18__733107-206-30-61?v=Thermo+Fisher
    Average 99 stars, based on 1 article reviews
    staining buffer - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    foxp3 staining buffer set  (Miltenyi Biotec)
    97
    Miltenyi Biotec foxp3 staining buffer set
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
    Foxp3 Staining Buffer Set, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/pmc13191621-195-6-10?v=Miltenyi+Biotec
    Average 97 stars, based on 1 article reviews
    foxp3 staining buffer set - by Bioz Stars, 2026-08
    97/100 stars
    		  Buy from Supplier
    staining buffer  (Fisher Scientific)
    86
    Fisher Scientific staining buffer
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
    Staining Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/bio_rxiv__64898__2026__06__03__729735-986-8-20?v=Fisher+Scientific
    Average 86 stars, based on 1 article reviews
    staining buffer - by Bioz Stars, 2026-08
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

    Techniques: Flow Cytometry, Isolation, Staining