Review



maxpar nuclear antigen staining buffer  (fluidigm)


Bioz Verified Symbol fluidigm is a verified supplier
Bioz Manufacturer Symbol fluidigm manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    fluidigm maxpar nuclear antigen staining buffer
    Maxpar Nuclear Antigen Staining Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/Maxpar+Nuclear+Antigen+Staining+Buffer+Set/pmc07779788-273-13-19
    Average 95 stars, based on 86 article reviews
    maxpar nuclear antigen staining buffer - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Maxpar Cell Staining Buffer Standard BioTools Cat # 201068 DPBS Corning Cat # 21-031-CV 16 % Formaldehyde (w/v), methanol-free Thermo Scientific Cat # 28906 Fetal Bovine Serum (FBS) Sigma Aldrich Cat # F7524 Maxpar Nuclear Antigen Staining Buffer Set Standard BioTools Cat # 201063 Maxpar Fix I Buffer Standard BioTools Cat # 201065 Maxpar 10X Barcode Perm Buffer Standard BioTools Cat # 201057 Cell-IDTM Intercalator-Iridium Standard BioTools Cat # 201192A Maxpar Water Standard BioTools Cat # 201069 Maxpar Cell Acquisition Solution Plus for CyTOF XT Standard BioTools Cat # 201244 EQTM Six Element Calibration Beads Standard BioTools Cat # 201245 Critical commercial assays MACS Neural Tissue Dissociation Kit P Miltenyi Biotec Inc. Cat # 130-092-628 RNeasy Mini Kit QIAGEN Cat # 74104 Neural Tissue Dissociation Kit (P) Miltenyi Biotec Inc. Cat # 130-092-628 Cell-ID 20-Plex Pd Barcoding Kit Standard BioTools Cat # 201060 Deposited data RNA-seq Florio et al.35 NCBI GEO: GSE65000 CUT&Tag Human tissue GW12-14 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 CUT&Tag PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Bulk RNA-seq PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Epi-CyTOF This paper FlowRepository: FR-FCM-Z8DC Experimental models: Cell lines Human: CRTDi004-A iPSC Völkner et al.78 CRTDi004-A; RRID: CVCL_YR23 Human: HPSI0114i-kolf_2 iPSC Welcome Trust Sanger Institute, HipSci WTSIi018-B; RRID: CVCL_AE29 Oligonucleotides Primers, see Table S7 This paper N/A Software and algorithms Fiji/ImageJ Fiji/ImageJ https://imagej.nih.gov/ij/ Prism (8.4.3) GraphPad software N/A Affinity Photo + Designer (1.10.5.1342) Serif Ltd. N/A FACSDiva (8.0.2) BD Biosciences N/A FACSChorus BD Biosciences N/A CyTOF Software (v9.0.2) Standard BioTools N/A OMIQ Dotmatics N/A FastQC (v0.11.9) Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ nf-core/cutandrun (v3.2.2) Cheshire et al.79 N/A Diffbind (v3.12.0) Stark et al.80 N/A DESeq2 (v1.42.1) Love et al.81 N/A deeptools (v3.5.1) Yu et al.82 N/A ChIPseeker (v1.38.0) Yu et al.82 and Bioconductor Core Team and Bioconductor Package Maintainer83 N/A ReactomePA (v1.46.0) Yu et al.44 and Moore et al.45 N/A (Continued on next page) e4 Neuron 113, 2927–2944.e1–e10, September 17, 2025 ..

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Article Title: GLYATL1 is associated with metabolic and epigenetic changes and with endocrine resistance in luminal breast cancer
    Article Snippet: .. Cells were washed with Maxpar Cell Staining Buffer (#201068, Fluidigm, San Francisco, CA, USA), and about 3 × 10 6 cells per sample were fixed, permeabilized using the Maxpar Nuclear Antigen Staining Buffer Set (#201063, Fluidigm, San Francisco, CA, USA), and barcoded with the Cell-ID 20-Plex Pd Barcoding Kit (#201060, Fluidigm, San Francisco, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: .. After centrifugation at 900 x g for 10 min, the pellets were resuspended in Maxpar Nuclear Antigen Staining buffer working solution (Mixed 1 part concentrated, 3 parts diluted, Standard BioTools, 201063) and incubated for 30 min at RT for permeabilization. .. Afterwards, the cells were washed twice by 2 ml Maxpar Nuclear Antigen Staining Perm (NP) buffer (Standard BioTools, 201063), with centrifugations at 900 x g for 10 min. Next, the cell pellets were resuspended in 1X Fix I Buffer (Standard BioTools, 201065) diluted in PBS and incubated for 10 min at RT, followed by a centrifugation at 900 x g for 10 min.

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: After centrifugation at 900 x g for 10 min, the pellets were resuspended in Maxpar Nuclear Antigen Staining buffer working solution (Mixed 1 part concentrated, 3 parts diluted, Standard BioTools, 201063) and incubated for 30 min at RT for permeabilization. .. Afterwards, the cells were washed twice by 2 ml Maxpar Nuclear Antigen Staining Perm (NP) buffer (Standard BioTools, 201063), with centrifugations at 900 x g for 10 min. Next, the cell pellets were resuspended in 1X Fix I Buffer (Standard BioTools, 201065) diluted in PBS and incubated for 10 min at RT, followed by a centrifugation at 900 x g for 10 min. .. The cells were washed twice with 1 ml 1X Barcode Perm Buffer (Standard BioTools, 201057).

    Article Title: Tumor immune dynamics and long-term clinical outcome of stage IIIA NSCLC patients treated with neoadjuvant chemoimmunotherapy
    Article Snippet: .. Cells were washed twice with CSB and permeabilized with the Maxpar Nuclear Antigen Staining Buffer Set (cat. 201063, Standard BioTools). ..

    Article Title: Development of a First-in-Class Click Chemistry–Based Cancer Therapeutic, from Preclinical Evaluation to a First-in-Human Dose Escalation Clinical Trial
    Article Snippet: A 1.6% formaldehyde (Thermo Fisher Scientific) solution was prepared with Maxpar PBS (Standard BioTools) as the diluent, the cells were fresh-fixed for 10 minutes at room temperature, spun at 800 × g for 5 minutes, and the supernatant was aspirated. .. For the samples stained with the functional panel, a 1× Nuclear Antigen Staining Buffer (Standard BioTools) was prepared from a stock solution, and cells were incubated in 1 mL for 30 minutes at room temperature. .. Cells were then washed with 2-mL Nuclear Antigen Staining Perm (Standard BioTools), spun at 800 × g for 5 minutes, and the supernatant was aspirated; this was repeated for two washes.

    Magnetic Cell Separation:

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Maxpar Cell Staining Buffer Standard BioTools Cat # 201068 DPBS Corning Cat # 21-031-CV 16 % Formaldehyde (w/v), methanol-free Thermo Scientific Cat # 28906 Fetal Bovine Serum (FBS) Sigma Aldrich Cat # F7524 Maxpar Nuclear Antigen Staining Buffer Set Standard BioTools Cat # 201063 Maxpar Fix I Buffer Standard BioTools Cat # 201065 Maxpar 10X Barcode Perm Buffer Standard BioTools Cat # 201057 Cell-IDTM Intercalator-Iridium Standard BioTools Cat # 201192A Maxpar Water Standard BioTools Cat # 201069 Maxpar Cell Acquisition Solution Plus for CyTOF XT Standard BioTools Cat # 201244 EQTM Six Element Calibration Beads Standard BioTools Cat # 201245 Critical commercial assays MACS Neural Tissue Dissociation Kit P Miltenyi Biotec Inc. Cat # 130-092-628 RNeasy Mini Kit QIAGEN Cat # 74104 Neural Tissue Dissociation Kit (P) Miltenyi Biotec Inc. Cat # 130-092-628 Cell-ID 20-Plex Pd Barcoding Kit Standard BioTools Cat # 201060 Deposited data RNA-seq Florio et al.35 NCBI GEO: GSE65000 CUT&Tag Human tissue GW12-14 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 CUT&Tag PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Bulk RNA-seq PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Epi-CyTOF This paper FlowRepository: FR-FCM-Z8DC Experimental models: Cell lines Human: CRTDi004-A iPSC Völkner et al.78 CRTDi004-A; RRID: CVCL_YR23 Human: HPSI0114i-kolf_2 iPSC Welcome Trust Sanger Institute, HipSci WTSIi018-B; RRID: CVCL_AE29 Oligonucleotides Primers, see Table S7 This paper N/A Software and algorithms Fiji/ImageJ Fiji/ImageJ https://imagej.nih.gov/ij/ Prism (8.4.3) GraphPad software N/A Affinity Photo + Designer (1.10.5.1342) Serif Ltd. N/A FACSDiva (8.0.2) BD Biosciences N/A FACSChorus BD Biosciences N/A CyTOF Software (v9.0.2) Standard BioTools N/A OMIQ Dotmatics N/A FastQC (v0.11.9) Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ nf-core/cutandrun (v3.2.2) Cheshire et al.79 N/A Diffbind (v3.12.0) Stark et al.80 N/A DESeq2 (v1.42.1) Love et al.81 N/A deeptools (v3.5.1) Yu et al.82 N/A ChIPseeker (v1.38.0) Yu et al.82 and Bioconductor Core Team and Bioconductor Package Maintainer83 N/A ReactomePA (v1.46.0) Yu et al.44 and Moore et al.45 N/A (Continued on next page) e4 Neuron 113, 2927–2944.e1–e10, September 17, 2025 ..

    RNA Sequencing:

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Maxpar Cell Staining Buffer Standard BioTools Cat # 201068 DPBS Corning Cat # 21-031-CV 16 % Formaldehyde (w/v), methanol-free Thermo Scientific Cat # 28906 Fetal Bovine Serum (FBS) Sigma Aldrich Cat # F7524 Maxpar Nuclear Antigen Staining Buffer Set Standard BioTools Cat # 201063 Maxpar Fix I Buffer Standard BioTools Cat # 201065 Maxpar 10X Barcode Perm Buffer Standard BioTools Cat # 201057 Cell-IDTM Intercalator-Iridium Standard BioTools Cat # 201192A Maxpar Water Standard BioTools Cat # 201069 Maxpar Cell Acquisition Solution Plus for CyTOF XT Standard BioTools Cat # 201244 EQTM Six Element Calibration Beads Standard BioTools Cat # 201245 Critical commercial assays MACS Neural Tissue Dissociation Kit P Miltenyi Biotec Inc. Cat # 130-092-628 RNeasy Mini Kit QIAGEN Cat # 74104 Neural Tissue Dissociation Kit (P) Miltenyi Biotec Inc. Cat # 130-092-628 Cell-ID 20-Plex Pd Barcoding Kit Standard BioTools Cat # 201060 Deposited data RNA-seq Florio et al.35 NCBI GEO: GSE65000 CUT&Tag Human tissue GW12-14 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 CUT&Tag PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Bulk RNA-seq PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Epi-CyTOF This paper FlowRepository: FR-FCM-Z8DC Experimental models: Cell lines Human: CRTDi004-A iPSC Völkner et al.78 CRTDi004-A; RRID: CVCL_YR23 Human: HPSI0114i-kolf_2 iPSC Welcome Trust Sanger Institute, HipSci WTSIi018-B; RRID: CVCL_AE29 Oligonucleotides Primers, see Table S7 This paper N/A Software and algorithms Fiji/ImageJ Fiji/ImageJ https://imagej.nih.gov/ij/ Prism (8.4.3) GraphPad software N/A Affinity Photo + Designer (1.10.5.1342) Serif Ltd. N/A FACSDiva (8.0.2) BD Biosciences N/A FACSChorus BD Biosciences N/A CyTOF Software (v9.0.2) Standard BioTools N/A OMIQ Dotmatics N/A FastQC (v0.11.9) Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ nf-core/cutandrun (v3.2.2) Cheshire et al.79 N/A Diffbind (v3.12.0) Stark et al.80 N/A DESeq2 (v1.42.1) Love et al.81 N/A deeptools (v3.5.1) Yu et al.82 N/A ChIPseeker (v1.38.0) Yu et al.82 and Bioconductor Core Team and Bioconductor Package Maintainer83 N/A ReactomePA (v1.46.0) Yu et al.44 and Moore et al.45 N/A (Continued on next page) e4 Neuron 113, 2927–2944.e1–e10, September 17, 2025 ..

    Software:

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Maxpar Cell Staining Buffer Standard BioTools Cat # 201068 DPBS Corning Cat # 21-031-CV 16 % Formaldehyde (w/v), methanol-free Thermo Scientific Cat # 28906 Fetal Bovine Serum (FBS) Sigma Aldrich Cat # F7524 Maxpar Nuclear Antigen Staining Buffer Set Standard BioTools Cat # 201063 Maxpar Fix I Buffer Standard BioTools Cat # 201065 Maxpar 10X Barcode Perm Buffer Standard BioTools Cat # 201057 Cell-IDTM Intercalator-Iridium Standard BioTools Cat # 201192A Maxpar Water Standard BioTools Cat # 201069 Maxpar Cell Acquisition Solution Plus for CyTOF XT Standard BioTools Cat # 201244 EQTM Six Element Calibration Beads Standard BioTools Cat # 201245 Critical commercial assays MACS Neural Tissue Dissociation Kit P Miltenyi Biotec Inc. Cat # 130-092-628 RNeasy Mini Kit QIAGEN Cat # 74104 Neural Tissue Dissociation Kit (P) Miltenyi Biotec Inc. Cat # 130-092-628 Cell-ID 20-Plex Pd Barcoding Kit Standard BioTools Cat # 201060 Deposited data RNA-seq Florio et al.35 NCBI GEO: GSE65000 CUT&Tag Human tissue GW12-14 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 CUT&Tag PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Bulk RNA-seq PRC2 inhibitor treated organoids W8 This paper Zenodo: https://doi.org/10.5281/ zenodo.13897222 Epi-CyTOF This paper FlowRepository: FR-FCM-Z8DC Experimental models: Cell lines Human: CRTDi004-A iPSC Völkner et al.78 CRTDi004-A; RRID: CVCL_YR23 Human: HPSI0114i-kolf_2 iPSC Welcome Trust Sanger Institute, HipSci WTSIi018-B; RRID: CVCL_AE29 Oligonucleotides Primers, see Table S7 This paper N/A Software and algorithms Fiji/ImageJ Fiji/ImageJ https://imagej.nih.gov/ij/ Prism (8.4.3) GraphPad software N/A Affinity Photo + Designer (1.10.5.1342) Serif Ltd. N/A FACSDiva (8.0.2) BD Biosciences N/A FACSChorus BD Biosciences N/A CyTOF Software (v9.0.2) Standard BioTools N/A OMIQ Dotmatics N/A FastQC (v0.11.9) Babraham Bioinformatics https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ nf-core/cutandrun (v3.2.2) Cheshire et al.79 N/A Diffbind (v3.12.0) Stark et al.80 N/A DESeq2 (v1.42.1) Love et al.81 N/A deeptools (v3.5.1) Yu et al.82 N/A ChIPseeker (v1.38.0) Yu et al.82 and Bioconductor Core Team and Bioconductor Package Maintainer83 N/A ReactomePA (v1.46.0) Yu et al.44 and Moore et al.45 N/A (Continued on next page) e4 Neuron 113, 2927–2944.e1–e10, September 17, 2025 ..

    Over Expression:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Microarray:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Fluorescence:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Microscopy:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Transfection:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Blocking Assay:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Cytometry:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Activation Assay:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Centrifugation:

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: .. After centrifugation at 900 x g for 10 min, the pellets were resuspended in Maxpar Nuclear Antigen Staining buffer working solution (Mixed 1 part concentrated, 3 parts diluted, Standard BioTools, 201063) and incubated for 30 min at RT for permeabilization. .. Afterwards, the cells were washed twice by 2 ml Maxpar Nuclear Antigen Staining Perm (NP) buffer (Standard BioTools, 201063), with centrifugations at 900 x g for 10 min. Next, the cell pellets were resuspended in 1X Fix I Buffer (Standard BioTools, 201065) diluted in PBS and incubated for 10 min at RT, followed by a centrifugation at 900 x g for 10 min.

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: After centrifugation at 900 x g for 10 min, the pellets were resuspended in Maxpar Nuclear Antigen Staining buffer working solution (Mixed 1 part concentrated, 3 parts diluted, Standard BioTools, 201063) and incubated for 30 min at RT for permeabilization. .. Afterwards, the cells were washed twice by 2 ml Maxpar Nuclear Antigen Staining Perm (NP) buffer (Standard BioTools, 201063), with centrifugations at 900 x g for 10 min. Next, the cell pellets were resuspended in 1X Fix I Buffer (Standard BioTools, 201065) diluted in PBS and incubated for 10 min at RT, followed by a centrifugation at 900 x g for 10 min. .. The cells were washed twice with 1 ml 1X Barcode Perm Buffer (Standard BioTools, 201057).

    Incubation:

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: .. After centrifugation at 900 x g for 10 min, the pellets were resuspended in Maxpar Nuclear Antigen Staining buffer working solution (Mixed 1 part concentrated, 3 parts diluted, Standard BioTools, 201063) and incubated for 30 min at RT for permeabilization. .. Afterwards, the cells were washed twice by 2 ml Maxpar Nuclear Antigen Staining Perm (NP) buffer (Standard BioTools, 201063), with centrifugations at 900 x g for 10 min. Next, the cell pellets were resuspended in 1X Fix I Buffer (Standard BioTools, 201065) diluted in PBS and incubated for 10 min at RT, followed by a centrifugation at 900 x g for 10 min.

    Article Title: Epigenome profiling identifies H3K27me3 regulation of extracellular matrix composition in human corticogenesis.
    Article Snippet: After centrifugation at 900 x g for 10 min, the pellets were resuspended in Maxpar Nuclear Antigen Staining buffer working solution (Mixed 1 part concentrated, 3 parts diluted, Standard BioTools, 201063) and incubated for 30 min at RT for permeabilization. .. Afterwards, the cells were washed twice by 2 ml Maxpar Nuclear Antigen Staining Perm (NP) buffer (Standard BioTools, 201063), with centrifugations at 900 x g for 10 min. Next, the cell pellets were resuspended in 1X Fix I Buffer (Standard BioTools, 201065) diluted in PBS and incubated for 10 min at RT, followed by a centrifugation at 900 x g for 10 min. .. The cells were washed twice with 1 ml 1X Barcode Perm Buffer (Standard BioTools, 201057).

    Article Title: Development of a First-in-Class Click Chemistry–Based Cancer Therapeutic, from Preclinical Evaluation to a First-in-Human Dose Escalation Clinical Trial
    Article Snippet: A 1.6% formaldehyde (Thermo Fisher Scientific) solution was prepared with Maxpar PBS (Standard BioTools) as the diluent, the cells were fresh-fixed for 10 minutes at room temperature, spun at 800 × g for 5 minutes, and the supernatant was aspirated. .. For the samples stained with the functional panel, a 1× Nuclear Antigen Staining Buffer (Standard BioTools) was prepared from a stock solution, and cells were incubated in 1 mL for 30 minutes at room temperature. .. Cells were then washed with 2-mL Nuclear Antigen Staining Perm (Standard BioTools), spun at 800 × g for 5 minutes, and the supernatant was aspirated; this was repeated for two washes.

    Functional Assay:

    Article Title: Development of a First-in-Class Click Chemistry–Based Cancer Therapeutic, from Preclinical Evaluation to a First-in-Human Dose Escalation Clinical Trial
    Article Snippet: A 1.6% formaldehyde (Thermo Fisher Scientific) solution was prepared with Maxpar PBS (Standard BioTools) as the diluent, the cells were fresh-fixed for 10 minutes at room temperature, spun at 800 × g for 5 minutes, and the supernatant was aspirated. .. For the samples stained with the functional panel, a 1× Nuclear Antigen Staining Buffer (Standard BioTools) was prepared from a stock solution, and cells were incubated in 1 mL for 30 minutes at room temperature. .. Cells were then washed with 2-mL Nuclear Antigen Staining Perm (Standard BioTools), spun at 800 × g for 5 minutes, and the supernatant was aspirated; this was repeated for two washes.



    Similar Products

    transcription factor staining buffer  (Miltenyi Biotec)
    97
    Miltenyi Biotec transcription factor staining buffer
    Transcription Factor Staining Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/Transcription+Factor+Staining+Buffer+Set/pmc13379542-331-6-12
    Average 97 stars, based on 1 article reviews
    transcription factor staining buffer - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    cells  (Miltenyi Biotec)
    97
    Miltenyi Biotec cells
    Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/FoxP3+Staining+Buffer+Set/pm42401537-343-3-9
    Average 97 stars, based on 1 article reviews
    cells - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    fox p3 buffer kit  (Miltenyi Biotec)
    97
    Miltenyi Biotec fox p3 buffer kit
    Fox P3 Buffer Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/FoxP3+Staining+Buffer+Set/bio_rxiv__64898__2026__06__30__735615-137-1-5
    Average 97 stars, based on 1 article reviews
    fox p3 buffer kit - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    foxp3 staining buffer  (Miltenyi Biotec)
    97
    Miltenyi Biotec foxp3 staining buffer
    Foxp3 Staining Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/FoxP3+Staining+Buffer+Set/pm42331382-367-13-19
    Average 97 stars, based on 1 article reviews
    foxp3 staining buffer - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    flow cytometry staining buffer  (Thermo Fisher)
    97
    Thermo Fisher flow cytometry staining buffer
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
    Flow Cytometry Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/Matrix+DeepWell+Storage+Blocks/pmc13068824-328-4-9
    Average 97 stars, based on 1 article reviews
    flow cytometry staining buffer - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    staining buffer  (Thermo Fisher)
    99
    Thermo Fisher staining buffer
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
    Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/Triton+X-100/bio_rxiv__64898__2026__06__18__733107-206-30-61
    Average 99 stars, based on 1 article reviews
    staining buffer - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    foxp3 staining buffer set  (Miltenyi Biotec)
    97
    Miltenyi Biotec foxp3 staining buffer set
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
    Foxp3 Staining Buffer Set, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/FoxP3+Staining+Buffer+Set/pmc13191621-195-6-10
    Average 97 stars, based on 1 article reviews
    foxp3 staining buffer set - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    staining buffer  (Fisher Scientific)
    86
    Fisher Scientific staining buffer
    Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
    Staining Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/buffer+factor+foxp3+set+staining+transcription/bio_rxiv__64898__2026__06__03__729735-986-8-20
    Average 86 stars, based on 1 article reviews
    staining buffer - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

    Techniques: Flow Cytometry, Isolation, Staining