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citrate buffer  (Aviva Systems)


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    Structured Review

    Aviva Systems citrate buffer
    Citrate Buffer, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/Cell+Staining+Buffer(500+mL)/pm35857834-254-18-27
    Average 90 stars, based on 1 article reviews
    citrate buffer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Noncanonical activation of GLI signaling in SOX2 + cells drives medulloblastoma relapse
    Article Snippet: .. Antigen retrieval on mouse-derived tissues or an MB tissue array (Biomax, BC17012c) was performed by steaming samples in citrate buffer for 30 min before staining for GLI2 (Aviva Systems Biology), GFAP, Ki67, SOX2 (Abcam), or cleaved caspase-3 (Cell Signaling Technology) according to the manufacturer’s recommendations. .. For GLI1 (Invitrogen) IHC staining, samples were boiled in EDTA (pH 8.5) for 10 min instead.

    Article Title: Noncanonical activation of GLI signaling in SOX2 + cells drives medulloblastoma relapse.
    Article Snippet: .. Antigen retrieval on mouse-derived tissues or an MB tissue array (Biomax, BC17012c) was performed by steaming samples in citrate buffer for 30 min before staining for GLI2 (Aviva Systems Biology), GFAP, Ki67, SOX2 (Abcam), or cleaved caspase-3 (Cell Signaling Technology) according to the manufacturer’s recommendations. .. For GLI1 (Invitrogen) IHC staining, samples were boiled in EDTA (pH 8.5) for 10 min instead.

    Article Title: Smarcd3 is an epigenetic modulator of the metabolic landscape in pancreatic ductal adenocarcinoma
    Article Snippet: IHC staining was performed on a Leica BOND RX automated immunostainer using BOND primary antibody diluent and BOND Polymer Refine DAB Detection kit per manufacturer’s instructions (Leica Biosystems). .. Pre-treatment was performed using citrate buffer at 100 °C for 30 min, and tissue was stained using rabbit anti-human Smarcd3 antibody (Aviva Systems Biology). .. Slides were scanned using a Pannoramic P250 digital slide scanner (3DHistech).

    Blocking Assay:

    Article Title: Helicase-like transcription factor confers radiation resistance in cervical cancer through enhancing the DNA damage repair capacity.
    Article Snippet: Helicase-like transcription factor (HLTF) is a member of the SWI/SNF (mating type switching/sucrose non-fermenting) family of ATPases/helicases and also has a RING-finger motif characteristic of ubiquitin ligase proteins.. These features have led to suggestions that HLTF functions like yeast Rad5, which promotes replication through DNA lesions via a post-replication repair pathway.. However, the function of HLTF in higher eukaryotes is still unknown.

    Incubation:

    Article Title: Helicase-like transcription factor confers radiation resistance in cervical cancer through enhancing the DNA damage repair capacity.
    Article Snippet: Helicase-like transcription factor (HLTF) is a member of the SWI/SNF (mating type switching/sucrose non-fermenting) family of ATPases/helicases and also has a RING-finger motif characteristic of ubiquitin ligase proteins.. These features have led to suggestions that HLTF functions like yeast Rad5, which promotes replication through DNA lesions via a post-replication repair pathway.. However, the function of HLTF in higher eukaryotes is still unknown.

    Article Title: Rosiglitazone blocks first trimester in-vitro placental injury caused by NF-κB-mediated inflammation
    Article Snippet: Purified protein (30 μg) was separated on 12% SDS-polyacrylamide gel (TGX Stain Free Fastcast Acrylamide kit, Bio-Rad Laboratories, CA) and transferred on PVDF membrane (Bio-Rad Laboratories, USA). .. Membranes were blocked with 5% skimmed milk in Tris-buffer saline (TBST) containing 0.1% (v/v) Tween for 1 hr at room temperature (RT) and incubated over night at 4 °C with the anti-GCM1 antibody (1:3000) (Aviva System Biology, UK). .. The membranes were washed with TBST and incubated with a HRP-conjugated secondary antibody (Cell signaling) for 1 hr. at RT.

    Immunohistochemistry:

    Article Title: Early regulative ability of the neuroepithelium to form cardiac neural crest
    Article Snippet: After labeling, the embryos were imaged using a Leica MZFLIII microscope, an X-cite series 120 fluorescent lamp and an RT Color Spot camera (Diagnostic Instruments, Inc). .. Immunohistochemistry Embryos were fixed in 4% paraformaldehyde for 20 min at room temperature for stage 7 embryos or for 24 hours at 4°C for older embryos, extensively washed in buffer (PBS + 0.1% Tween) and blocked in antibody buffer (PBS + 0.1% Tween + 5% (by volume) goat serum + 0.2% BSA) for one hour before the primary antibody Pax7 (Aviva System Biology; diluted 1:1 in antibody buffer) was used to recognize the dorsal neural tube, pre-migratory and migrating neural crest and somitic tissue. ..

    Saline:

    Article Title: Rosiglitazone blocks first trimester in-vitro placental injury caused by NF-κB-mediated inflammation
    Article Snippet: Purified protein (30 μg) was separated on 12% SDS-polyacrylamide gel (TGX Stain Free Fastcast Acrylamide kit, Bio-Rad Laboratories, CA) and transferred on PVDF membrane (Bio-Rad Laboratories, USA). .. Membranes were blocked with 5% skimmed milk in Tris-buffer saline (TBST) containing 0.1% (v/v) Tween for 1 hr at room temperature (RT) and incubated over night at 4 °C with the anti-GCM1 antibody (1:3000) (Aviva System Biology, UK). .. The membranes were washed with TBST and incubated with a HRP-conjugated secondary antibody (Cell signaling) for 1 hr. at RT.

    other:

    Article Title: Smarcd3 is an epigenetic modulator of the metabolic landscape in pancreatic ductal adenocarcinoma.
    Article Snippet: Slides were scanned using a Pannoramic P250 digital slide scanner (3DHistech).



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    Image Search Results


    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

    Techniques: Flow Cytometry, Isolation, Staining