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jc 1 staining buffer  (Beyotime)


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    Structured Review

    Beyotime jc 1 staining buffer
    Jc 1 Staining Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 15614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+buffer/JC-1/pm42878994-97-5-9
    Average 99 stars, based on 15614 article reviews
    jc 1 staining buffer - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Targeting the Calcium-mtDNA-cGAS-STING Signaling Nexus: A Novel Redox Mechanism for Nimodipine's Neuroprotection in Traumatic Brain Injury.
    Article Snippet: .. Mitochondrial membrane potential detection via JC-1 probe DWm was measured using the JC-1 probe (C2006, Beyotime, China). ..

    Article Title: Curcumin analogue FLLL32 induces ferroptosis by targeting the STAT3-GPX4/SLC40A1 axis in colorectal cancer
    Article Snippet: Apoptosis was determined with an Annexin V-FITC Apoptosis Detection Kit (Beyotime, C1062). .. The mitochondrial membrane potential (MMP) was determined with a JC-1 assay kit (Beyotime, C2006). .. Reactive oxygen species (ROS) were analyzed using a ROS assay kit (Beyotime, S0033S).

    Article Title: STAT3/MTND4 Mediates Obesity-Related Seminal Vesicle Injury and Impaired Sperm Motility Via Reactive Oxygen Species /Mitochondrial Dysfunction.
    Article Snippet: 1 Center of Reproduction, Women and Children’s Hospital Affiliated to Ningbo University, No. 339 Liuting Street, Haishu District, Ningbo City, Zhejiang Province 315012, China 2 Internal Medicine, Women and Children’s Hospital Affiliated to Ningbo University, No. 339 Liuting Street, Haishu District, Ningbo City, Zhejiang Province 315012, China Abstract Obesity induces male reproductive dysfunction.. Mitochondrial dysfunction ranks among the mechanisms driving functional impairment.. Notably, Mitochondrial NADH dehydrogenase subunit 4 (MTND4) exerts a pivotal role in sustaining the function of the mitochondrial electron transport chain and cellular energy metabolism.

    Article Title: Lysosomal protein transmembrane 5 protects against myocardial ischemia-reperfusion injury by promoting mitophagy via the NEDD4-LC3 axis.
    Article Snippet: .. Mitochondrial membrane potential (ΔΨm) was evaluated with the JC-1 kit (Beyotime, C2006). ..

    Article Title: Bacterial stimulation triggers IRF7-mediated IFN-λ production in hepatocytes to attenuate sepsis.
    Article Snippet: Total superoxide dismutase (SOD) activity was determined using a Total Superoxide Dismutase Assay Kit (WST-8 method, S0101, Beyotime). .. Mitochondrial membrane potential (JC-1 staining) was assessed with a kit (C2003S, Beyotime) by staining the cells, followed by detection with a fluorescence spectrophotometer. .. 2.18 shRNA mediated knockdown of Ifnlr1 in cells AR TIC LE IN PR ES S shRNAs targeting mouse Ifnlr1 were designed and synthesized by GenePharma (Shanghai, China).

    Article Title: Neuronal oxidative stress and mitophagy contribute to microglial inflammation in radiation-induced brain injury.
    Article Snippet: Radiation-induced brain injury is a severe complication of cranial radiotherapy, but the redoxdependent mechanisms linking neuronal damage to microglial neuroinflammation remain incompletely understood.. Here, we investigated whether radiation-induced reactive oxygen species (ROS) are associated with neuronal mitochondrial dysfunction, lipid accumulation, and subsequent microglial inflammatory activation.. Using an irradiated mouse brain injury model and neuron– microglia conditioned-medium systems, we found that ionizing radiation increased neuronal ROS production and impaired mitochondrial homeostasis, accompanied by enhanced autophagic turnover and mitophagy-related changes.

    Incubation:

    Article Title: Icariin Protects Against Mitochondrial Damage and Ferroptosis-Related Injury in Synovial Fibroblast-Like Synoviocytes Through SUMO1-Dependent Regulation of SIRT1.
    Article Snippet: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation and progressive joint destruction, in which fibroblastlike synoviocytes (FLSs) play a central pathogenic role.. Ferroptosis and oxidative stressdriven mitochondrial dysfunction have recently been implicated in synovial cell injury during RA progression.. Sirtuin 1 (SIRT1), a nicotinamide adenine dinucleotidedependent deacetylase, is known to regulate inflammation, redox homeostasis, and cell survival; however, the upstream regulatory mechanisms controlling SIRT1 function and their relevance to ferroptosis in RA remain poorly defined.

    Mitochondrial Membrane Potential Assay:

    Article Title: Icariin Protects Against Mitochondrial Damage and Ferroptosis-Related Injury in Synovial Fibroblast-Like Synoviocytes Through SUMO1-Dependent Regulation of SIRT1.
    Article Snippet: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation and progressive joint destruction, in which fibroblastlike synoviocytes (FLSs) play a central pathogenic role.. Ferroptosis and oxidative stressdriven mitochondrial dysfunction have recently been implicated in synovial cell injury during RA progression.. Sirtuin 1 (SIRT1), a nicotinamide adenine dinucleotidedependent deacetylase, is known to regulate inflammation, redox homeostasis, and cell survival; however, the upstream regulatory mechanisms controlling SIRT1 function and their relevance to ferroptosis in RA remain poorly defined.

    Article Title: STAT3/MTND4 Mediates Obesity-Related Seminal Vesicle Injury and Impaired Sperm Motility Via Reactive Oxygen Species /Mitochondrial Dysfunction.
    Article Snippet: 1 Center of Reproduction, Women and Children’s Hospital Affiliated to Ningbo University, No. 339 Liuting Street, Haishu District, Ningbo City, Zhejiang Province 315012, China 2 Internal Medicine, Women and Children’s Hospital Affiliated to Ningbo University, No. 339 Liuting Street, Haishu District, Ningbo City, Zhejiang Province 315012, China Abstract Obesity induces male reproductive dysfunction.. Mitochondrial dysfunction ranks among the mechanisms driving functional impairment.. Notably, Mitochondrial NADH dehydrogenase subunit 4 (MTND4) exerts a pivotal role in sustaining the function of the mitochondrial electron transport chain and cellular energy metabolism.

    Article Title: Bacterial stimulation triggers IRF7-mediated IFN-λ production in hepatocytes to attenuate sepsis.
    Article Snippet: Total superoxide dismutase (SOD) activity was determined using a Total Superoxide Dismutase Assay Kit (WST-8 method, S0101, Beyotime). .. Mitochondrial membrane potential (JC-1 staining) was assessed with a kit (C2003S, Beyotime) by staining the cells, followed by detection with a fluorescence spectrophotometer. .. 2.18 shRNA mediated knockdown of Ifnlr1 in cells AR TIC LE IN PR ES S shRNAs targeting mouse Ifnlr1 were designed and synthesized by GenePharma (Shanghai, China).

    Article Title: A Mitochondria-Modulating Nanosystem for Rheumatoid Arthritis Immunotherapy via Inflammatory Cascade Disruption.
    Article Snippet: Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic inflammation within joints, where macrophage-driven inflammatory cascade caused by mitochondrial dysfunction significantly contributes to the pathogenesis.. Through recruitment and activation of neutrophils from circulation, these macrophages promote the release of neutrophil extracellular traps (NETs) and generation of reactive oxygen species (ROS), leading to synovitis exacerbation and tissue damage.. To disrupt this closely linked cycle, we present a mitochondria-modulating nanosystem (NM@APDAase) targeting the inflammatory cascade for RA immunotherapy.

    Staining:

    Article Title: Bacterial stimulation triggers IRF7-mediated IFN-λ production in hepatocytes to attenuate sepsis.
    Article Snippet: Total superoxide dismutase (SOD) activity was determined using a Total Superoxide Dismutase Assay Kit (WST-8 method, S0101, Beyotime). .. Mitochondrial membrane potential (JC-1 staining) was assessed with a kit (C2003S, Beyotime) by staining the cells, followed by detection with a fluorescence spectrophotometer. .. 2.18 shRNA mediated knockdown of Ifnlr1 in cells AR TIC LE IN PR ES S shRNAs targeting mouse Ifnlr1 were designed and synthesized by GenePharma (Shanghai, China).

    Fluorescence:

    Article Title: Bacterial stimulation triggers IRF7-mediated IFN-λ production in hepatocytes to attenuate sepsis.
    Article Snippet: Total superoxide dismutase (SOD) activity was determined using a Total Superoxide Dismutase Assay Kit (WST-8 method, S0101, Beyotime). .. Mitochondrial membrane potential (JC-1 staining) was assessed with a kit (C2003S, Beyotime) by staining the cells, followed by detection with a fluorescence spectrophotometer. .. 2.18 shRNA mediated knockdown of Ifnlr1 in cells AR TIC LE IN PR ES S shRNAs targeting mouse Ifnlr1 were designed and synthesized by GenePharma (Shanghai, China).

    Spectrophotometry:

    Article Title: Bacterial stimulation triggers IRF7-mediated IFN-λ production in hepatocytes to attenuate sepsis.
    Article Snippet: Total superoxide dismutase (SOD) activity was determined using a Total Superoxide Dismutase Assay Kit (WST-8 method, S0101, Beyotime). .. Mitochondrial membrane potential (JC-1 staining) was assessed with a kit (C2003S, Beyotime) by staining the cells, followed by detection with a fluorescence spectrophotometer. .. 2.18 shRNA mediated knockdown of Ifnlr1 in cells AR TIC LE IN PR ES S shRNAs targeting mouse Ifnlr1 were designed and synthesized by GenePharma (Shanghai, China).



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