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custom built zeiss axio observer 3 microscope stage  (Carl Zeiss)


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    Structured Review

    Carl Zeiss custom built zeiss axio observer 3 microscope stage
    Custom Built Zeiss Axio Observer 3 Microscope Stage, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stage+microscope/Microscope+stand+Axio+Observer+3/pmc13049531-392-7-8
    Average 99 stars, based on 1523 article reviews
    custom built zeiss axio observer 3 microscope stage - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: The landscape and regulatory potential of eccDNAs in mammalian preimplantation embryos.
    Article Snippet: .. Images were captured using an Axio Observer 3 microscope (Zeiss) with ZEN Blue Lite imaging software. .. Multiple displacement amplification (MDA), library construction and sequencing Following the manufacturer’s recommendation, whole metagenome amplification was performed on 6 samples (zygote, 2-Cell, 4-Cell, 8-Cell, morula and blastocyst) using MDA with the REPLIg Single Cell kit (Qiagen, 150345).

    Article Title: The phosphodiesterase-5 inhibitor vardenafil reverses sleep deprivation-induced amnesia in mice
    Article Snippet: .. Fluorescent images were obtained using a Zeiss AXIO Observer microscope and analyzed in QuPath (v.0.4.3). ..

    Article Title: Protocol for monitoring cytoplasmic Ca 2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope.
    Article Snippet: .. This table provides a quick reference for researchers wishing to replicate the setup on a Zeiss Axio Observer microscope. .. Timing: 1 h Here we describe steps for using the Zeiss Axio Oberver and the ZEN software.

    Article Title: Elucidating motion patterns in sperm cell motion with dynamic mode decomposition.
    Article Snippet: .. Bovine sperm cell density after thawing and washing was 107 cells/mL, and was reduced to about 106 cells/mL for imaging to avoid excessive density and crossing trajectories during recording.Videoswere recorded immediately after washing, at room temperature and atmospheric pressure, using a Zeiss Axio Observer microscope equipped with phase contrast and a Basler high-speed camera. ..

    Article Title: Elucidating motion patterns in sperm cell motion with dynamic mode decomposition
    Article Snippet: .. Videos were recorded immediately after washing, at room temperature and atmospheric pressure, using a Zeiss Axio Observer microscope equipped with phase contrast and a Basler high-speed camera. ..

    Article Title: Ex-vivo culture of human hypertrophic cardiomyopathy hearts: Functional and metabolic changes during long-term culture
    Article Snippet: .. Briefly, our chambers were placed on a custom-built Zeiss Axio Observer 3 microscope stage, where the sarcomere length of the cardiac strips was assessed using the brightfield mode. ..

    Article Title: Sex‐specific metabolic responses to glucagon receptor agonism and modulation of the FGF21‐glucagon axis in female mice
    Article Snippet: .. Images of slides stained with haematoxylin-eosin were obtained using a Zeiss Axio Observer microscope with an Axiocam 702 camera at 10× magnification. ..

    Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice
    Article Snippet: For cytotoxicity assessment, cells were seeded in 96-well plates (1 × 10 4 cells/well) and exposed to various concentrations of PC capsules (0.03–0.25 mg/mL) for 24 h. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay (M2128, Sigma, Germany), with absorbance measured at 490 nm. .. Parallel live/dead staining was performed using calcein-AM/PI (40747ES80, Yeasen, China) on cells cultured in 48-well plates (1.5 × 10 4 cells/well), with fluorescence imaging conducted using a Zeiss Axio Observer3 microscope. ..

    Imaging:

    Article Title: The landscape and regulatory potential of eccDNAs in mammalian preimplantation embryos.
    Article Snippet: .. Images were captured using an Axio Observer 3 microscope (Zeiss) with ZEN Blue Lite imaging software. .. Multiple displacement amplification (MDA), library construction and sequencing Following the manufacturer’s recommendation, whole metagenome amplification was performed on 6 samples (zygote, 2-Cell, 4-Cell, 8-Cell, morula and blastocyst) using MDA with the REPLIg Single Cell kit (Qiagen, 150345).

    Article Title: Elucidating motion patterns in sperm cell motion with dynamic mode decomposition.
    Article Snippet: .. Bovine sperm cell density after thawing and washing was 107 cells/mL, and was reduced to about 106 cells/mL for imaging to avoid excessive density and crossing trajectories during recording.Videoswere recorded immediately after washing, at room temperature and atmospheric pressure, using a Zeiss Axio Observer microscope equipped with phase contrast and a Basler high-speed camera. ..

    Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice
    Article Snippet: For cytotoxicity assessment, cells were seeded in 96-well plates (1 × 10 4 cells/well) and exposed to various concentrations of PC capsules (0.03–0.25 mg/mL) for 24 h. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay (M2128, Sigma, Germany), with absorbance measured at 490 nm. .. Parallel live/dead staining was performed using calcein-AM/PI (40747ES80, Yeasen, China) on cells cultured in 48-well plates (1.5 × 10 4 cells/well), with fluorescence imaging conducted using a Zeiss Axio Observer3 microscope. ..

    Software:

    Article Title: The landscape and regulatory potential of eccDNAs in mammalian preimplantation embryos.
    Article Snippet: .. Images were captured using an Axio Observer 3 microscope (Zeiss) with ZEN Blue Lite imaging software. .. Multiple displacement amplification (MDA), library construction and sequencing Following the manufacturer’s recommendation, whole metagenome amplification was performed on 6 samples (zygote, 2-Cell, 4-Cell, 8-Cell, morula and blastocyst) using MDA with the REPLIg Single Cell kit (Qiagen, 150345).

    Staining:

    Article Title: Sex‐specific metabolic responses to glucagon receptor agonism and modulation of the FGF21‐glucagon axis in female mice
    Article Snippet: .. Images of slides stained with haematoxylin-eosin were obtained using a Zeiss Axio Observer microscope with an Axiocam 702 camera at 10× magnification. ..

    Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice
    Article Snippet: For cytotoxicity assessment, cells were seeded in 96-well plates (1 × 10 4 cells/well) and exposed to various concentrations of PC capsules (0.03–0.25 mg/mL) for 24 h. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay (M2128, Sigma, Germany), with absorbance measured at 490 nm. .. Parallel live/dead staining was performed using calcein-AM/PI (40747ES80, Yeasen, China) on cells cultured in 48-well plates (1.5 × 10 4 cells/well), with fluorescence imaging conducted using a Zeiss Axio Observer3 microscope. ..

    Cell Culture:

    Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice
    Article Snippet: For cytotoxicity assessment, cells were seeded in 96-well plates (1 × 10 4 cells/well) and exposed to various concentrations of PC capsules (0.03–0.25 mg/mL) for 24 h. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay (M2128, Sigma, Germany), with absorbance measured at 490 nm. .. Parallel live/dead staining was performed using calcein-AM/PI (40747ES80, Yeasen, China) on cells cultured in 48-well plates (1.5 × 10 4 cells/well), with fluorescence imaging conducted using a Zeiss Axio Observer3 microscope. ..

    Fluorescence:

    Article Title: Procyanidin capsules attenuate PI3K/AKT-mediated mitochondrial dysfunction and accelerate skin wound healing in diabetic mice
    Article Snippet: For cytotoxicity assessment, cells were seeded in 96-well plates (1 × 10 4 cells/well) and exposed to various concentrations of PC capsules (0.03–0.25 mg/mL) for 24 h. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay (M2128, Sigma, Germany), with absorbance measured at 490 nm. .. Parallel live/dead staining was performed using calcein-AM/PI (40747ES80, Yeasen, China) on cells cultured in 48-well plates (1.5 × 10 4 cells/well), with fluorescence imaging conducted using a Zeiss Axio Observer3 microscope. ..



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    Image Search Results


    A . 2P microscope equipped with a DMD-based pattern stimulation setup and wide field cameras for alignment calibration. Camera 1 was positioned in the conjugate plane of the DMD and was used for alignment of the DMD projected patterns to the 2 P imaging. Camera 2 and Objective 2 are temporarily placed below the sample plane and used to characterize the axial spread of projected patterns. DM - dichoric mirror. B . Left : Images of illuminated areas of different size square patches (NxN) of active DMD pixels taken by Camera 1. Right: Estimated and measured full-width half-maximum (FWHM) of illuminated areas for different patch sizes. C . Left: Images of projected patterns of 20×20 DMD pixels at different positions of the Objective 1 taken by Camera 2. Right: Intensity profiles of projected patterns along the x-axis. Di . 2 P images of the glomerular layer with manually drawn glomerular ROIs. Dii . Corresponding DMD mask patterns; Diii . Image of the glomerular mask pattern with Camera 1. Div . Images of the glomerular mask pattern with Camera 2 at different position of the Objective 1.

    Journal: Nature Neuroscience

    Article Title: Rapid temporal processing in the olfactory bulb underlies concentration-invariant odor identification and signal decorrelation

    doi: 10.1038/s41593-026-02250-y

    Figure Lengend Snippet: A . 2P microscope equipped with a DMD-based pattern stimulation setup and wide field cameras for alignment calibration. Camera 1 was positioned in the conjugate plane of the DMD and was used for alignment of the DMD projected patterns to the 2 P imaging. Camera 2 and Objective 2 are temporarily placed below the sample plane and used to characterize the axial spread of projected patterns. DM - dichoric mirror. B . Left : Images of illuminated areas of different size square patches (NxN) of active DMD pixels taken by Camera 1. Right: Estimated and measured full-width half-maximum (FWHM) of illuminated areas for different patch sizes. C . Left: Images of projected patterns of 20×20 DMD pixels at different positions of the Objective 1 taken by Camera 2. Right: Intensity profiles of projected patterns along the x-axis. Di . 2 P images of the glomerular layer with manually drawn glomerular ROIs. Dii . Corresponding DMD mask patterns; Diii . Image of the glomerular mask pattern with Camera 1. Div . Images of the glomerular mask pattern with Camera 2 at different position of the Objective 1.

    Article Snippet: This algorithm attempted to minimize the difference between the reference image and the FOV by iteratively moving the microscope stage (Thorlabs, cat. no. PLS-XY) to reduce the residual displacement computed using a rigid motion correction package (NoRMCorre, Flatiron Institute) .

    Techniques: Microscopy, Imaging