Review




Structured Review

Brickell Biotech ssc buffer
Ssc Buffer, supplied by Brickell Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/buffer+ssc/pm41936701-318-15-17
Average 86 stars, based on 1 article reviews
ssc buffer - by Bioz Stars, 2026-10
86/100 stars

Images

Related Articles

In Situ:

Article Title: Cochlear nucleus spatial transcriptomes of normal and hearing loss mice reveal a critical role of Spp1 in bushy cells.
Article Snippet: Images of the stained CNs were captured using a Motic Custom PA53 FS6 microscope prior to in situ capture of fluorescein isothiocyanate signals (objective 10×). .. In situ reverse transcription, amplification, library construction and sequencing We washed tissue sections using 0.1× SSC buffer (BBI, B548109-0200) supplemented with 0.05 U/μL RNase inhibitor (NEB, M0314L). .. Tissue sections placed on the chip were permeabilized with PR Enzyme (STOmics Gene Expression kit S1) in 0.01 M HCl buffer at 37 °C for 12min, and then washed with 0.1× SSC buffer supplemented with 0.05 U/ μL RNase inhibitor. mRNAs captured by the DNBs were reverse transcribed using SuperScript II Reverse Transcription mix (STOmics Gene Expression Kit S1, containing RT Reagent, RT Additive, RT Oligo, ReverseT Enzyme, and 0.05 U/μL RNase inhibitor) at 42 °C for 1 h. After reverse transcription, cDNAs on the chip were amplified using KAPA HiFi HotStart Ready Mix (Roche, KK2602) with 0.8 μM cDNA-PCR primers.

Reverse Transcription:

Article Title: Cochlear nucleus spatial transcriptomes of normal and hearing loss mice reveal a critical role of Spp1 in bushy cells.
Article Snippet: Images of the stained CNs were captured using a Motic Custom PA53 FS6 microscope prior to in situ capture of fluorescein isothiocyanate signals (objective 10×). .. In situ reverse transcription, amplification, library construction and sequencing We washed tissue sections using 0.1× SSC buffer (BBI, B548109-0200) supplemented with 0.05 U/μL RNase inhibitor (NEB, M0314L). .. Tissue sections placed on the chip were permeabilized with PR Enzyme (STOmics Gene Expression kit S1) in 0.01 M HCl buffer at 37 °C for 12min, and then washed with 0.1× SSC buffer supplemented with 0.05 U/ μL RNase inhibitor. mRNAs captured by the DNBs were reverse transcribed using SuperScript II Reverse Transcription mix (STOmics Gene Expression Kit S1, containing RT Reagent, RT Additive, RT Oligo, ReverseT Enzyme, and 0.05 U/μL RNase inhibitor) at 42 °C for 1 h. After reverse transcription, cDNAs on the chip were amplified using KAPA HiFi HotStart Ready Mix (Roche, KK2602) with 0.8 μM cDNA-PCR primers.

Amplification:

Article Title: Cochlear nucleus spatial transcriptomes of normal and hearing loss mice reveal a critical role of Spp1 in bushy cells.
Article Snippet: Images of the stained CNs were captured using a Motic Custom PA53 FS6 microscope prior to in situ capture of fluorescein isothiocyanate signals (objective 10×). .. In situ reverse transcription, amplification, library construction and sequencing We washed tissue sections using 0.1× SSC buffer (BBI, B548109-0200) supplemented with 0.05 U/μL RNase inhibitor (NEB, M0314L). .. Tissue sections placed on the chip were permeabilized with PR Enzyme (STOmics Gene Expression kit S1) in 0.01 M HCl buffer at 37 °C for 12min, and then washed with 0.1× SSC buffer supplemented with 0.05 U/ μL RNase inhibitor. mRNAs captured by the DNBs were reverse transcribed using SuperScript II Reverse Transcription mix (STOmics Gene Expression Kit S1, containing RT Reagent, RT Additive, RT Oligo, ReverseT Enzyme, and 0.05 U/μL RNase inhibitor) at 42 °C for 1 h. After reverse transcription, cDNAs on the chip were amplified using KAPA HiFi HotStart Ready Mix (Roche, KK2602) with 0.8 μM cDNA-PCR primers.

Sequencing:

Article Title: Cochlear nucleus spatial transcriptomes of normal and hearing loss mice reveal a critical role of Spp1 in bushy cells.
Article Snippet: Images of the stained CNs were captured using a Motic Custom PA53 FS6 microscope prior to in situ capture of fluorescein isothiocyanate signals (objective 10×). .. In situ reverse transcription, amplification, library construction and sequencing We washed tissue sections using 0.1× SSC buffer (BBI, B548109-0200) supplemented with 0.05 U/μL RNase inhibitor (NEB, M0314L). .. Tissue sections placed on the chip were permeabilized with PR Enzyme (STOmics Gene Expression kit S1) in 0.01 M HCl buffer at 37 °C for 12min, and then washed with 0.1× SSC buffer supplemented with 0.05 U/ μL RNase inhibitor. mRNAs captured by the DNBs were reverse transcribed using SuperScript II Reverse Transcription mix (STOmics Gene Expression Kit S1, containing RT Reagent, RT Additive, RT Oligo, ReverseT Enzyme, and 0.05 U/μL RNase inhibitor) at 42 °C for 1 h. After reverse transcription, cDNAs on the chip were amplified using KAPA HiFi HotStart Ready Mix (Roche, KK2602) with 0.8 μM cDNA-PCR primers.



Similar Products

97
Thermo Fisher saline sodium citrate 20x
Saline Sodium Citrate 20x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/pmc13264319-41-0-5
Average 97 stars, based on 1 article reviews
saline sodium citrate 20x - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

97
Thermo Fisher ssc buffer rnase free
Ssc Buffer Rnase Free, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/pmc13293986-80-1-4
Average 97 stars, based on 1 article reviews
ssc buffer rnase free - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

97
Thermo Fisher saline sodium citrate ssc buffer
Saline Sodium Citrate Ssc Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/pmc13197786-24-0-6
Average 97 stars, based on 1 article reviews
saline sodium citrate ssc buffer - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

97
Thermo Fisher 2xssc buffer
2xssc Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/pm42252663-201-6-26
Average 97 stars, based on 1 article reviews
2xssc buffer - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

97
Thermo Fisher buffer h
Buffer H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/bio_rxiv__64898__2026__05__14__724355-235-22-33
Average 97 stars, based on 1 article reviews
buffer h - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

97
Thermo Fisher ssc buffer 203 thermo fisher scientific am9770
Ssc Buffer 203 Thermo Fisher Scientific Am9770, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/pm42140188-233-193-196
Average 97 stars, based on 1 article reviews
ssc buffer 203 thermo fisher scientific am9770 - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

97
Thermo Fisher ssc buffer
Ssc Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/pmc13109722-112-15-17
Average 97 stars, based on 1 article reviews
ssc buffer - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

86
Fluka Chemical ssc buffer solution
Ssc Buffer Solution, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/buffer+solution+ssc/pm42076203-45-11-20
Average 86 stars, based on 1 article reviews
ssc buffer solution - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

97
Thermo Fisher 2x sodium chloride sodium citrate ssc buffer
2x Sodium Chloride Sodium Citrate Ssc Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/SSC+buffer/pm42061735-119-66-73
Average 97 stars, based on 1 article reviews
2x sodium chloride sodium citrate ssc buffer - by Bioz Stars, 2026-10
97/100 stars
  Buy from Supplier

Image Search Results