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Promega 2× ssc buffer
2× Ssc Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/2%C3%97+ssc+buffer/pm33225471-78-6-8
Average 90 stars, based on 1 article reviews
2× ssc buffer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Amplification:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Control:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Recombinant:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Plasmid Preparation:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Software:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

DNA Purification:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Staining:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Clinical Proteomics:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Concentration Assay:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Incubation:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.

Hybridization:

Article Title: Polystyrene nanoplastics as an ecotoxicological hazard: cellular and transcriptomic evidences on marine and freshwater in vitro teleost models.
Article Snippet: The reaction was stopped by adding the 2× SSC buffer (Promega).

Article Title: Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro.
Article Snippet: Then, slides were submerged in 2× SSC buffer (Promega) for 15 min, washed three times with PBS for 5 min each and analysed at 1,000× using an epifluorescence microscope with excitation and emission filters at 488 and 530 nm, respectively.

Article Title: Three-dimensional imaging for the quantification of spatial patterns in microbiota of the intestinal mucosa
Article Snippet: Samples were “pre-hybridized” in a solution of 2xSSC (#V4261, saline sodium citrate, Promega Corp., WI, USA) and 10% dextran sulfate sodium (#D8906, Sigma, MO, USA) for 1 h at room temperature.


Article Title: Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Article Snippet: Air-dried samples were washed briefly in 2× SSC buffer (Promega) and then dehydrated in ascending ethanol series (70%, 85% and 100%) each for 2 min. For GBM39 cells, Cytocell EGFR amplification Probe (OGT) targeting both EGFR and D7Z1 (centromeric probe as a control for chromosome 7) was added to the slide and a coverslip was applied.

Article Title: Accurate Prediction of ecDNA in Interphase Cancer Cells using Deep Neural Networks
Article Snippet: After gently removing the coverslip sealant, the slides were immersed in 2x SSC/0.1% Tween-20 (V4261, Promega Corp.) for 3 minutes in the dark.

Article Title: piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.
Article Snippet: piRNAs (PIWI-interacting RNAs) are a class of small non-coding RNAs (ncRNAs) abundantly expressed in germline cells and involved in suppressing the transposon activity.. Interestingly, recent studies have found piRNA expression in the central nervous system (CNS), yet the underlying biological significance remains largely unknown.. In this study, we investigated the expression and function of piRNAs during the retinoic acid (RA)-mediated neuronal differentiation in NT2 cells, a human embryonal carcinoma cell line.



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