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inducible adipocyte specific srf knockout srf ako mice  (Jackson Laboratory)

 
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    Structured Review

    Jackson Laboratory inducible adipocyte specific srf knockout srf ako mice
    Role of <t>SRF</t> in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and <t>tamoxifen-inducible,</t> <t>adipocyte-specific</t> Srf KO <t>(SRF-AKO,</t> n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Inducible Adipocyte Specific Srf Knockout Srf Ako Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice"

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    Journal: Metabolism: clinical and experimental

    doi: 10.1016/j.metabol.2026.156548

    Role of SRF in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and tamoxifen-inducible, adipocyte-specific Srf KO (SRF-AKO, n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: Role of SRF in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and tamoxifen-inducible, adipocyte-specific Srf KO (SRF-AKO, n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: In Vivo, Expressing, Staining, Isolation, Two Tailed Test

    Impaired systemic glucose homeostasis and partial lipodystrophy phenotype in SRF-AKO mice during obesity. (A–C) Defective glucose homeostasis in SRF-AKO ( n = 9) compared to WT ( n = 11) male mice during obesity (total N = 20), assessed by (A) glucose tolerance test (GTT, 1 g/kg body weight glucose), (B) insulin tolerance test (ITT, 1 U/kg body weight insulin), and (C) fasting insulin levels (ng/mL) relative to body weight. (D) Tissue weight (% of body weight) of eWAT, iWAT, and BAT, and liver in WT ( n = 11) and SRF-AKO ( n = 9) male mice. Total N = 20. (E–F) Representative H&E-stained sections of (E) BAT and (F) liver from WT and SRF-AKO male mice. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: Impaired systemic glucose homeostasis and partial lipodystrophy phenotype in SRF-AKO mice during obesity. (A–C) Defective glucose homeostasis in SRF-AKO ( n = 9) compared to WT ( n = 11) male mice during obesity (total N = 20), assessed by (A) glucose tolerance test (GTT, 1 g/kg body weight glucose), (B) insulin tolerance test (ITT, 1 U/kg body weight insulin), and (C) fasting insulin levels (ng/mL) relative to body weight. (D) Tissue weight (% of body weight) of eWAT, iWAT, and BAT, and liver in WT ( n = 11) and SRF-AKO ( n = 9) male mice. Total N = 20. (E–F) Representative H&E-stained sections of (E) BAT and (F) liver from WT and SRF-AKO male mice. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.

    Techniques Used: Staining, Two Tailed Test

    Compromised structural integrity and increased cellular fragility in SRF-deficient adipocytes. (A) Basal and isoproterenol (ISO)-stimulated lipolysis, measured by glycerol release from 4-h eWAT and iWAT explants from WT and SRF-AKO male mice ( n = 3 per condition, total N = 12). (B) Representative transmission electron microscopy (TEM) images of eWAT from WT and SRF-AKO female mice, showing ruptured adipocyte membranes (indicated by black arrows). Scale bar: 5 μm. (C) Quantification of apoptotic cells in eWAT and iWAT from WT (eWAT, n = 3; iWAT, n = 5) and SRF-AKO (eWAT, n = 3; iWAT, n = 3) male mice by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. (D) BODIPY staining of eWAT and iWAT from WT and SRF-AKO male mice following 1.5-h of compression. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: Compromised structural integrity and increased cellular fragility in SRF-deficient adipocytes. (A) Basal and isoproterenol (ISO)-stimulated lipolysis, measured by glycerol release from 4-h eWAT and iWAT explants from WT and SRF-AKO male mice ( n = 3 per condition, total N = 12). (B) Representative transmission electron microscopy (TEM) images of eWAT from WT and SRF-AKO female mice, showing ruptured adipocyte membranes (indicated by black arrows). Scale bar: 5 μm. (C) Quantification of apoptotic cells in eWAT and iWAT from WT (eWAT, n = 3; iWAT, n = 5) and SRF-AKO (eWAT, n = 3; iWAT, n = 3) male mice by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. (D) BODIPY staining of eWAT and iWAT from WT and SRF-AKO male mice following 1.5-h of compression. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.

    Techniques Used: Transmission Assay, Electron Microscopy, TUNEL Assay, Staining, Two Tailed Test

    Impaired vascular integrity and altered cell-cell communication in adipose tissue driven by loss of SRF in adipocytes. (A) UMAP visualization of 34,457 single nuclei isolated from eWAT and iWAT of WT and SRF-AKO male mice (total N = 4), with annotated cell types. (B) Violin plots of cell type-specific marker gene expression across all identified cell types. (C) Relative proportions of each cell type in eWAT and iWAT from WT and SRF-AKO male mice. (D–E) Whole-mount staining of eWAT from WT and SRF-AKO mice with Hoechst (blue), BODIPY (green), and either F4/80 (red, D) or PECAM1 (red, E). Scale bar: 100 μm. (F) Circle plots from CellChat analysis showing altered cell-cell communication in eWAT of SRF-AKO male mouse compared to WT. Increases in interaction number (left) and strength (right) are shown in red; decreases are shown in blue.
    Figure Legend Snippet: Impaired vascular integrity and altered cell-cell communication in adipose tissue driven by loss of SRF in adipocytes. (A) UMAP visualization of 34,457 single nuclei isolated from eWAT and iWAT of WT and SRF-AKO male mice (total N = 4), with annotated cell types. (B) Violin plots of cell type-specific marker gene expression across all identified cell types. (C) Relative proportions of each cell type in eWAT and iWAT from WT and SRF-AKO male mice. (D–E) Whole-mount staining of eWAT from WT and SRF-AKO mice with Hoechst (blue), BODIPY (green), and either F4/80 (red, D) or PECAM1 (red, E). Scale bar: 100 μm. (F) Circle plots from CellChat analysis showing altered cell-cell communication in eWAT of SRF-AKO male mouse compared to WT. Increases in interaction number (left) and strength (right) are shown in red; decreases are shown in blue.

    Techniques Used: Isolation, Marker, Gene Expression, Staining

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    BIA-KA:

    Article Title: Cell-type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Antibodies Streptavidin, HRP-linked Cell Signaling Cat. 3999S AB_10830897 anti-V5 tag Abcam Cat. ab206562 AB_3719924 anti-Human IgG, HRP-linked Abcam Cat. ab6858 AB_955433 anti-mAcrp30 (adiponectin) R&D Systems Cat. AF1119 AB_2221770 anti-Goat IgG, HRP-linked Thermo Fisher Cat. A15999 AB_2534673 Streptavidin, Alexa 647 BioLegend Cat. 405237 anti-mAlbumin Cell Signaling Cat. 4929S AB_2225785 anti-Rabbit IgG, HRP-linked Cell Signaling Cat. 7074S AB_2099233 anti-BTK Cell Signaling Cat. 8547S AB_10950506 anti-Ces1d Santa Cruz Biotechnology Cat. sc-374160 AB_10988772 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR Cat. 926-32210 AB_621842 anti-Complement C6 Invitrogen Cat. PA5-117369 AB_2901999 IRDye 680CW Goat anti-Rabbit IgG Secondary LI-COR Cat. 926-68071 AB_10956166 anti-SerpinA3N R&D Systems Cat. AF4709 AB_2270116 IRDye 680RD Donkey anti-Goat IgG Secondary LI-COR Cat. 926-68074 AB_10956736 IRDye 800CW Streptavidin LI-COR Cat. 926-32230 anti-mFactor D (Adipsin, CFAD) R&D Systems Cat. AF5430 AB_1655868 anti-LRG1 Sigma-Aldrich Cat. HPA001888 AB_1079276 anti-FABP4 R&D Systems Cat. AF1443-SP AB_2102444 anti-mRetinol binding protein 4 R&D Systems Cat. AF3476 AB_2167682 anti-Sheep IgG, HRP-linked Thermo Fisher Cat. A16041 AB_2534715 anti-Vinculin Cell Signaling Cat. 4650S AB_10559207 Chemicals, peptides, and recombinant proteins Biotin Sigma-Aldrich Cat. B4501 NP-40 Millipore Cat. 492016 cOmplete Protease Inhibitor Cocktail tablets Roche Cat. 04693159001 PhosphoSTOP Roche Cat. 04906837001 XT MES Buffer Bio-Rad Cat. 1610789 Criterion 4–12% Bis-Tris 12-well gels Bio-Rad Cat. 3450123 Tris/Glycine transfer Buffer Bio-Rad Cat. 1610771 SuperBlock T20 blocking buffer Thermo Scientific Cat. 37536 0.45 μm PVDF membrane Millipore Cat. IPVH00010 Enhanced Chemiluminescence (ECL) PerkinElmer Cat. NEL104001EA Paraformaldehyde Sigma-Aldrich Cat. P6148 Dichloromethane Fisher Scientific Cat. AA39116K2 Hydrogen peroxide, 30% Fisher Scientific Cat. H325100 Heparin Sigma-Aldrich Cat. H3393 Dibenzyl ether Sigma-Aldrich Cat. 108014 Pierce Streptavidin Magnetic Beads Thermo Scientific Cat. 88817 Urea Supelco Cat. 108487 Iodoacetamide Sigma-Aldrich Cat. I1149 Dithiothreitol (DTT) Fisher Scientific Cat. BP172-25 Trypsin, sequencing grade Promega Cat. V5111 Acetonitrile, LC/MS grade Fisher Scientific Cat. A955 (Continued on next page) Cell Reports 45, 117507, June 23, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Hydroxylamine solution Sigma-Aldrich Cat. 467804 EPPS Alfa Aesar Cat. A13714-22 TMTpro 16-plex Label Reagent Set Thermo Scientific Cat. A44521 Lipopolysaccharide (LPS) Sigma-Aldrich Cat. L2880 Nitrocellulose membrane, 0.2 μm Bio-Rad Cat. 1620112 Intercept (TBS) Blocking Buffer LI-COR Cat. 927-60001 D-(+)-Glucose Sigma-Aldrich Cat. G8270 Critical commercial assays DC assay kit Bio-Rad Cat. 5000111 BCA assay kit Thermo Scientific Cat. 23228 Pierce C18 pipette tips Thermo Scientific Cat. 87784 Sep-Pak Vac C18 cartridges Waters Cat. WAT054955 Glucose Meter, Glucose Strips Nova Max Plus Blood Glucose Monitoring System Cat. 8548043524 QuantiTect Reverse Transcription Kit QIAGEN Cat. 205311 SYBR GreenER qPCR SuperMix Invitrogen Cat. 11760500 Deposited data All raw proteomic data has been uploaded to PRIDE This paper PRIDE: PXD075539 Experimental Models: Organisms/strains TurboIDKDEL This paper Albumin-Cre (B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J) The Jackson Laboratory Strain 003574 Adiponectin-Cre (B6.FVB-Tg(Adipoq-cre) 1Evdr/J) The Jackson Laboratory Strain 028020 CD19-Cre (B6.129P2(C)-Cd19tm1(cre)Cgn/J) The Jackson Laboratory Strain 006785 Wild-type (C57BL/6J) The Jackson Laboratory Strain 000664 Recombinant DNA CTV plasmid Addgene Cat. 15912 Software and algorithms TurboID analysis pipeline This paper github.com/vinogradova-lab/ cell-type-specific-proximity-labeling IP2 and ProLuCID Integrated Proteomics Applications Xu et al., J Proteomics (2015)75 RawConverter (v1.2.0.1) Yates Lab, Scripps Research He et al., Anal Chem (2015)76 NumPy (v1.22.4) Van der Walt et al., 201077 numpy.org Pandas (v1.4.3) McKinney, 201078 pandas.pydata.org ggplot2 (v3.3.5) Wikham, 201679 ggplot2.tidyverse.org SignalP (v6.0) Emanuelsson, 200725 services.healthtech.dtu.dk/ services/SignalP-6.0/ ComplexHeatmap (v2.10.0) Gu, 202280 github.com/jokergoo/ ComplexHeatmap GOATOOLS (v1.3.1) Klopfenstein et al. 201881 github.com/tanghaibao/goatools scikit-learn (v1.0.2) Pedregosa et al., 201182 scikit-learn.org/stable ggrepel (v0.9.1) github.com/slowkow/ggrepel Image Lab Software (v6.1) Bio-Rad Other Rodent regular chow diet PicoLab Cat. 5053 Rodent diet with 60 kcal% Fat Research Diets Cat. D12492 Rodent water bottle nozzle, 30mm, short straight Vision Racks & Cages Type E Microvette EDTA capillary blood collection tubes Sarstedt Cat. 16.444.100 22 Cell Reports 45, 117507, June 23, 2026 ..

    Transferring:

    Article Title: Cell-type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Antibodies Streptavidin, HRP-linked Cell Signaling Cat. 3999S AB_10830897 anti-V5 tag Abcam Cat. ab206562 AB_3719924 anti-Human IgG, HRP-linked Abcam Cat. ab6858 AB_955433 anti-mAcrp30 (adiponectin) R&D Systems Cat. AF1119 AB_2221770 anti-Goat IgG, HRP-linked Thermo Fisher Cat. A15999 AB_2534673 Streptavidin, Alexa 647 BioLegend Cat. 405237 anti-mAlbumin Cell Signaling Cat. 4929S AB_2225785 anti-Rabbit IgG, HRP-linked Cell Signaling Cat. 7074S AB_2099233 anti-BTK Cell Signaling Cat. 8547S AB_10950506 anti-Ces1d Santa Cruz Biotechnology Cat. sc-374160 AB_10988772 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR Cat. 926-32210 AB_621842 anti-Complement C6 Invitrogen Cat. PA5-117369 AB_2901999 IRDye 680CW Goat anti-Rabbit IgG Secondary LI-COR Cat. 926-68071 AB_10956166 anti-SerpinA3N R&D Systems Cat. AF4709 AB_2270116 IRDye 680RD Donkey anti-Goat IgG Secondary LI-COR Cat. 926-68074 AB_10956736 IRDye 800CW Streptavidin LI-COR Cat. 926-32230 anti-mFactor D (Adipsin, CFAD) R&D Systems Cat. AF5430 AB_1655868 anti-LRG1 Sigma-Aldrich Cat. HPA001888 AB_1079276 anti-FABP4 R&D Systems Cat. AF1443-SP AB_2102444 anti-mRetinol binding protein 4 R&D Systems Cat. AF3476 AB_2167682 anti-Sheep IgG, HRP-linked Thermo Fisher Cat. A16041 AB_2534715 anti-Vinculin Cell Signaling Cat. 4650S AB_10559207 Chemicals, peptides, and recombinant proteins Biotin Sigma-Aldrich Cat. B4501 NP-40 Millipore Cat. 492016 cOmplete Protease Inhibitor Cocktail tablets Roche Cat. 04693159001 PhosphoSTOP Roche Cat. 04906837001 XT MES Buffer Bio-Rad Cat. 1610789 Criterion 4–12% Bis-Tris 12-well gels Bio-Rad Cat. 3450123 Tris/Glycine transfer Buffer Bio-Rad Cat. 1610771 SuperBlock T20 blocking buffer Thermo Scientific Cat. 37536 0.45 μm PVDF membrane Millipore Cat. IPVH00010 Enhanced Chemiluminescence (ECL) PerkinElmer Cat. NEL104001EA Paraformaldehyde Sigma-Aldrich Cat. P6148 Dichloromethane Fisher Scientific Cat. AA39116K2 Hydrogen peroxide, 30% Fisher Scientific Cat. H325100 Heparin Sigma-Aldrich Cat. H3393 Dibenzyl ether Sigma-Aldrich Cat. 108014 Pierce Streptavidin Magnetic Beads Thermo Scientific Cat. 88817 Urea Supelco Cat. 108487 Iodoacetamide Sigma-Aldrich Cat. I1149 Dithiothreitol (DTT) Fisher Scientific Cat. BP172-25 Trypsin, sequencing grade Promega Cat. V5111 Acetonitrile, LC/MS grade Fisher Scientific Cat. A955 (Continued on next page) Cell Reports 45, 117507, June 23, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Hydroxylamine solution Sigma-Aldrich Cat. 467804 EPPS Alfa Aesar Cat. A13714-22 TMTpro 16-plex Label Reagent Set Thermo Scientific Cat. A44521 Lipopolysaccharide (LPS) Sigma-Aldrich Cat. L2880 Nitrocellulose membrane, 0.2 μm Bio-Rad Cat. 1620112 Intercept (TBS) Blocking Buffer LI-COR Cat. 927-60001 D-(+)-Glucose Sigma-Aldrich Cat. G8270 Critical commercial assays DC assay kit Bio-Rad Cat. 5000111 BCA assay kit Thermo Scientific Cat. 23228 Pierce C18 pipette tips Thermo Scientific Cat. 87784 Sep-Pak Vac C18 cartridges Waters Cat. WAT054955 Glucose Meter, Glucose Strips Nova Max Plus Blood Glucose Monitoring System Cat. 8548043524 QuantiTect Reverse Transcription Kit QIAGEN Cat. 205311 SYBR GreenER qPCR SuperMix Invitrogen Cat. 11760500 Deposited data All raw proteomic data has been uploaded to PRIDE This paper PRIDE: PXD075539 Experimental Models: Organisms/strains TurboIDKDEL This paper Albumin-Cre (B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J) The Jackson Laboratory Strain 003574 Adiponectin-Cre (B6.FVB-Tg(Adipoq-cre) 1Evdr/J) The Jackson Laboratory Strain 028020 CD19-Cre (B6.129P2(C)-Cd19tm1(cre)Cgn/J) The Jackson Laboratory Strain 006785 Wild-type (C57BL/6J) The Jackson Laboratory Strain 000664 Recombinant DNA CTV plasmid Addgene Cat. 15912 Software and algorithms TurboID analysis pipeline This paper github.com/vinogradova-lab/ cell-type-specific-proximity-labeling IP2 and ProLuCID Integrated Proteomics Applications Xu et al., J Proteomics (2015)75 RawConverter (v1.2.0.1) Yates Lab, Scripps Research He et al., Anal Chem (2015)76 NumPy (v1.22.4) Van der Walt et al., 201077 numpy.org Pandas (v1.4.3) McKinney, 201078 pandas.pydata.org ggplot2 (v3.3.5) Wikham, 201679 ggplot2.tidyverse.org SignalP (v6.0) Emanuelsson, 200725 services.healthtech.dtu.dk/ services/SignalP-6.0/ ComplexHeatmap (v2.10.0) Gu, 202280 github.com/jokergoo/ ComplexHeatmap GOATOOLS (v1.3.1) Klopfenstein et al. 201881 github.com/tanghaibao/goatools scikit-learn (v1.0.2) Pedregosa et al., 201182 scikit-learn.org/stable ggrepel (v0.9.1) github.com/slowkow/ggrepel Image Lab Software (v6.1) Bio-Rad Other Rodent regular chow diet PicoLab Cat. 5053 Rodent diet with 60 kcal% Fat Research Diets Cat. D12492 Rodent water bottle nozzle, 30mm, short straight Vision Racks & Cages Type E Microvette EDTA capillary blood collection tubes Sarstedt Cat. 16.444.100 22 Cell Reports 45, 117507, June 23, 2026 ..

    Reverse Transcription:

    Article Title: Cell-type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Antibodies Streptavidin, HRP-linked Cell Signaling Cat. 3999S AB_10830897 anti-V5 tag Abcam Cat. ab206562 AB_3719924 anti-Human IgG, HRP-linked Abcam Cat. ab6858 AB_955433 anti-mAcrp30 (adiponectin) R&D Systems Cat. AF1119 AB_2221770 anti-Goat IgG, HRP-linked Thermo Fisher Cat. A15999 AB_2534673 Streptavidin, Alexa 647 BioLegend Cat. 405237 anti-mAlbumin Cell Signaling Cat. 4929S AB_2225785 anti-Rabbit IgG, HRP-linked Cell Signaling Cat. 7074S AB_2099233 anti-BTK Cell Signaling Cat. 8547S AB_10950506 anti-Ces1d Santa Cruz Biotechnology Cat. sc-374160 AB_10988772 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR Cat. 926-32210 AB_621842 anti-Complement C6 Invitrogen Cat. PA5-117369 AB_2901999 IRDye 680CW Goat anti-Rabbit IgG Secondary LI-COR Cat. 926-68071 AB_10956166 anti-SerpinA3N R&D Systems Cat. AF4709 AB_2270116 IRDye 680RD Donkey anti-Goat IgG Secondary LI-COR Cat. 926-68074 AB_10956736 IRDye 800CW Streptavidin LI-COR Cat. 926-32230 anti-mFactor D (Adipsin, CFAD) R&D Systems Cat. AF5430 AB_1655868 anti-LRG1 Sigma-Aldrich Cat. HPA001888 AB_1079276 anti-FABP4 R&D Systems Cat. AF1443-SP AB_2102444 anti-mRetinol binding protein 4 R&D Systems Cat. AF3476 AB_2167682 anti-Sheep IgG, HRP-linked Thermo Fisher Cat. A16041 AB_2534715 anti-Vinculin Cell Signaling Cat. 4650S AB_10559207 Chemicals, peptides, and recombinant proteins Biotin Sigma-Aldrich Cat. B4501 NP-40 Millipore Cat. 492016 cOmplete Protease Inhibitor Cocktail tablets Roche Cat. 04693159001 PhosphoSTOP Roche Cat. 04906837001 XT MES Buffer Bio-Rad Cat. 1610789 Criterion 4–12% Bis-Tris 12-well gels Bio-Rad Cat. 3450123 Tris/Glycine transfer Buffer Bio-Rad Cat. 1610771 SuperBlock T20 blocking buffer Thermo Scientific Cat. 37536 0.45 μm PVDF membrane Millipore Cat. IPVH00010 Enhanced Chemiluminescence (ECL) PerkinElmer Cat. NEL104001EA Paraformaldehyde Sigma-Aldrich Cat. P6148 Dichloromethane Fisher Scientific Cat. AA39116K2 Hydrogen peroxide, 30% Fisher Scientific Cat. H325100 Heparin Sigma-Aldrich Cat. H3393 Dibenzyl ether Sigma-Aldrich Cat. 108014 Pierce Streptavidin Magnetic Beads Thermo Scientific Cat. 88817 Urea Supelco Cat. 108487 Iodoacetamide Sigma-Aldrich Cat. I1149 Dithiothreitol (DTT) Fisher Scientific Cat. BP172-25 Trypsin, sequencing grade Promega Cat. V5111 Acetonitrile, LC/MS grade Fisher Scientific Cat. A955 (Continued on next page) Cell Reports 45, 117507, June 23, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Hydroxylamine solution Sigma-Aldrich Cat. 467804 EPPS Alfa Aesar Cat. A13714-22 TMTpro 16-plex Label Reagent Set Thermo Scientific Cat. A44521 Lipopolysaccharide (LPS) Sigma-Aldrich Cat. L2880 Nitrocellulose membrane, 0.2 μm Bio-Rad Cat. 1620112 Intercept (TBS) Blocking Buffer LI-COR Cat. 927-60001 D-(+)-Glucose Sigma-Aldrich Cat. G8270 Critical commercial assays DC assay kit Bio-Rad Cat. 5000111 BCA assay kit Thermo Scientific Cat. 23228 Pierce C18 pipette tips Thermo Scientific Cat. 87784 Sep-Pak Vac C18 cartridges Waters Cat. WAT054955 Glucose Meter, Glucose Strips Nova Max Plus Blood Glucose Monitoring System Cat. 8548043524 QuantiTect Reverse Transcription Kit QIAGEN Cat. 205311 SYBR GreenER qPCR SuperMix Invitrogen Cat. 11760500 Deposited data All raw proteomic data has been uploaded to PRIDE This paper PRIDE: PXD075539 Experimental Models: Organisms/strains TurboIDKDEL This paper Albumin-Cre (B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J) The Jackson Laboratory Strain 003574 Adiponectin-Cre (B6.FVB-Tg(Adipoq-cre) 1Evdr/J) The Jackson Laboratory Strain 028020 CD19-Cre (B6.129P2(C)-Cd19tm1(cre)Cgn/J) The Jackson Laboratory Strain 006785 Wild-type (C57BL/6J) The Jackson Laboratory Strain 000664 Recombinant DNA CTV plasmid Addgene Cat. 15912 Software and algorithms TurboID analysis pipeline This paper github.com/vinogradova-lab/ cell-type-specific-proximity-labeling IP2 and ProLuCID Integrated Proteomics Applications Xu et al., J Proteomics (2015)75 RawConverter (v1.2.0.1) Yates Lab, Scripps Research He et al., Anal Chem (2015)76 NumPy (v1.22.4) Van der Walt et al., 201077 numpy.org Pandas (v1.4.3) McKinney, 201078 pandas.pydata.org ggplot2 (v3.3.5) Wikham, 201679 ggplot2.tidyverse.org SignalP (v6.0) Emanuelsson, 200725 services.healthtech.dtu.dk/ services/SignalP-6.0/ ComplexHeatmap (v2.10.0) Gu, 202280 github.com/jokergoo/ ComplexHeatmap GOATOOLS (v1.3.1) Klopfenstein et al. 201881 github.com/tanghaibao/goatools scikit-learn (v1.0.2) Pedregosa et al., 201182 scikit-learn.org/stable ggrepel (v0.9.1) github.com/slowkow/ggrepel Image Lab Software (v6.1) Bio-Rad Other Rodent regular chow diet PicoLab Cat. 5053 Rodent diet with 60 kcal% Fat Research Diets Cat. D12492 Rodent water bottle nozzle, 30mm, short straight Vision Racks & Cages Type E Microvette EDTA capillary blood collection tubes Sarstedt Cat. 16.444.100 22 Cell Reports 45, 117507, June 23, 2026 ..

    Real-time Polymerase Chain Reaction:

    Article Title: Cell-type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Antibodies Streptavidin, HRP-linked Cell Signaling Cat. 3999S AB_10830897 anti-V5 tag Abcam Cat. ab206562 AB_3719924 anti-Human IgG, HRP-linked Abcam Cat. ab6858 AB_955433 anti-mAcrp30 (adiponectin) R&D Systems Cat. AF1119 AB_2221770 anti-Goat IgG, HRP-linked Thermo Fisher Cat. A15999 AB_2534673 Streptavidin, Alexa 647 BioLegend Cat. 405237 anti-mAlbumin Cell Signaling Cat. 4929S AB_2225785 anti-Rabbit IgG, HRP-linked Cell Signaling Cat. 7074S AB_2099233 anti-BTK Cell Signaling Cat. 8547S AB_10950506 anti-Ces1d Santa Cruz Biotechnology Cat. sc-374160 AB_10988772 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR Cat. 926-32210 AB_621842 anti-Complement C6 Invitrogen Cat. PA5-117369 AB_2901999 IRDye 680CW Goat anti-Rabbit IgG Secondary LI-COR Cat. 926-68071 AB_10956166 anti-SerpinA3N R&D Systems Cat. AF4709 AB_2270116 IRDye 680RD Donkey anti-Goat IgG Secondary LI-COR Cat. 926-68074 AB_10956736 IRDye 800CW Streptavidin LI-COR Cat. 926-32230 anti-mFactor D (Adipsin, CFAD) R&D Systems Cat. AF5430 AB_1655868 anti-LRG1 Sigma-Aldrich Cat. HPA001888 AB_1079276 anti-FABP4 R&D Systems Cat. AF1443-SP AB_2102444 anti-mRetinol binding protein 4 R&D Systems Cat. AF3476 AB_2167682 anti-Sheep IgG, HRP-linked Thermo Fisher Cat. A16041 AB_2534715 anti-Vinculin Cell Signaling Cat. 4650S AB_10559207 Chemicals, peptides, and recombinant proteins Biotin Sigma-Aldrich Cat. B4501 NP-40 Millipore Cat. 492016 cOmplete Protease Inhibitor Cocktail tablets Roche Cat. 04693159001 PhosphoSTOP Roche Cat. 04906837001 XT MES Buffer Bio-Rad Cat. 1610789 Criterion 4–12% Bis-Tris 12-well gels Bio-Rad Cat. 3450123 Tris/Glycine transfer Buffer Bio-Rad Cat. 1610771 SuperBlock T20 blocking buffer Thermo Scientific Cat. 37536 0.45 μm PVDF membrane Millipore Cat. IPVH00010 Enhanced Chemiluminescence (ECL) PerkinElmer Cat. NEL104001EA Paraformaldehyde Sigma-Aldrich Cat. P6148 Dichloromethane Fisher Scientific Cat. AA39116K2 Hydrogen peroxide, 30% Fisher Scientific Cat. H325100 Heparin Sigma-Aldrich Cat. H3393 Dibenzyl ether Sigma-Aldrich Cat. 108014 Pierce Streptavidin Magnetic Beads Thermo Scientific Cat. 88817 Urea Supelco Cat. 108487 Iodoacetamide Sigma-Aldrich Cat. I1149 Dithiothreitol (DTT) Fisher Scientific Cat. BP172-25 Trypsin, sequencing grade Promega Cat. V5111 Acetonitrile, LC/MS grade Fisher Scientific Cat. A955 (Continued on next page) Cell Reports 45, 117507, June 23, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Hydroxylamine solution Sigma-Aldrich Cat. 467804 EPPS Alfa Aesar Cat. A13714-22 TMTpro 16-plex Label Reagent Set Thermo Scientific Cat. A44521 Lipopolysaccharide (LPS) Sigma-Aldrich Cat. L2880 Nitrocellulose membrane, 0.2 μm Bio-Rad Cat. 1620112 Intercept (TBS) Blocking Buffer LI-COR Cat. 927-60001 D-(+)-Glucose Sigma-Aldrich Cat. G8270 Critical commercial assays DC assay kit Bio-Rad Cat. 5000111 BCA assay kit Thermo Scientific Cat. 23228 Pierce C18 pipette tips Thermo Scientific Cat. 87784 Sep-Pak Vac C18 cartridges Waters Cat. WAT054955 Glucose Meter, Glucose Strips Nova Max Plus Blood Glucose Monitoring System Cat. 8548043524 QuantiTect Reverse Transcription Kit QIAGEN Cat. 205311 SYBR GreenER qPCR SuperMix Invitrogen Cat. 11760500 Deposited data All raw proteomic data has been uploaded to PRIDE This paper PRIDE: PXD075539 Experimental Models: Organisms/strains TurboIDKDEL This paper Albumin-Cre (B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J) The Jackson Laboratory Strain 003574 Adiponectin-Cre (B6.FVB-Tg(Adipoq-cre) 1Evdr/J) The Jackson Laboratory Strain 028020 CD19-Cre (B6.129P2(C)-Cd19tm1(cre)Cgn/J) The Jackson Laboratory Strain 006785 Wild-type (C57BL/6J) The Jackson Laboratory Strain 000664 Recombinant DNA CTV plasmid Addgene Cat. 15912 Software and algorithms TurboID analysis pipeline This paper github.com/vinogradova-lab/ cell-type-specific-proximity-labeling IP2 and ProLuCID Integrated Proteomics Applications Xu et al., J Proteomics (2015)75 RawConverter (v1.2.0.1) Yates Lab, Scripps Research He et al., Anal Chem (2015)76 NumPy (v1.22.4) Van der Walt et al., 201077 numpy.org Pandas (v1.4.3) McKinney, 201078 pandas.pydata.org ggplot2 (v3.3.5) Wikham, 201679 ggplot2.tidyverse.org SignalP (v6.0) Emanuelsson, 200725 services.healthtech.dtu.dk/ services/SignalP-6.0/ ComplexHeatmap (v2.10.0) Gu, 202280 github.com/jokergoo/ ComplexHeatmap GOATOOLS (v1.3.1) Klopfenstein et al. 201881 github.com/tanghaibao/goatools scikit-learn (v1.0.2) Pedregosa et al., 201182 scikit-learn.org/stable ggrepel (v0.9.1) github.com/slowkow/ggrepel Image Lab Software (v6.1) Bio-Rad Other Rodent regular chow diet PicoLab Cat. 5053 Rodent diet with 60 kcal% Fat Research Diets Cat. D12492 Rodent water bottle nozzle, 30mm, short straight Vision Racks & Cages Type E Microvette EDTA capillary blood collection tubes Sarstedt Cat. 16.444.100 22 Cell Reports 45, 117507, June 23, 2026 ..

    Recombinant:

    Article Title: Cell-type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Antibodies Streptavidin, HRP-linked Cell Signaling Cat. 3999S AB_10830897 anti-V5 tag Abcam Cat. ab206562 AB_3719924 anti-Human IgG, HRP-linked Abcam Cat. ab6858 AB_955433 anti-mAcrp30 (adiponectin) R&D Systems Cat. AF1119 AB_2221770 anti-Goat IgG, HRP-linked Thermo Fisher Cat. A15999 AB_2534673 Streptavidin, Alexa 647 BioLegend Cat. 405237 anti-mAlbumin Cell Signaling Cat. 4929S AB_2225785 anti-Rabbit IgG, HRP-linked Cell Signaling Cat. 7074S AB_2099233 anti-BTK Cell Signaling Cat. 8547S AB_10950506 anti-Ces1d Santa Cruz Biotechnology Cat. sc-374160 AB_10988772 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR Cat. 926-32210 AB_621842 anti-Complement C6 Invitrogen Cat. PA5-117369 AB_2901999 IRDye 680CW Goat anti-Rabbit IgG Secondary LI-COR Cat. 926-68071 AB_10956166 anti-SerpinA3N R&D Systems Cat. AF4709 AB_2270116 IRDye 680RD Donkey anti-Goat IgG Secondary LI-COR Cat. 926-68074 AB_10956736 IRDye 800CW Streptavidin LI-COR Cat. 926-32230 anti-mFactor D (Adipsin, CFAD) R&D Systems Cat. AF5430 AB_1655868 anti-LRG1 Sigma-Aldrich Cat. HPA001888 AB_1079276 anti-FABP4 R&D Systems Cat. AF1443-SP AB_2102444 anti-mRetinol binding protein 4 R&D Systems Cat. AF3476 AB_2167682 anti-Sheep IgG, HRP-linked Thermo Fisher Cat. A16041 AB_2534715 anti-Vinculin Cell Signaling Cat. 4650S AB_10559207 Chemicals, peptides, and recombinant proteins Biotin Sigma-Aldrich Cat. B4501 NP-40 Millipore Cat. 492016 cOmplete Protease Inhibitor Cocktail tablets Roche Cat. 04693159001 PhosphoSTOP Roche Cat. 04906837001 XT MES Buffer Bio-Rad Cat. 1610789 Criterion 4–12% Bis-Tris 12-well gels Bio-Rad Cat. 3450123 Tris/Glycine transfer Buffer Bio-Rad Cat. 1610771 SuperBlock T20 blocking buffer Thermo Scientific Cat. 37536 0.45 μm PVDF membrane Millipore Cat. IPVH00010 Enhanced Chemiluminescence (ECL) PerkinElmer Cat. NEL104001EA Paraformaldehyde Sigma-Aldrich Cat. P6148 Dichloromethane Fisher Scientific Cat. AA39116K2 Hydrogen peroxide, 30% Fisher Scientific Cat. H325100 Heparin Sigma-Aldrich Cat. H3393 Dibenzyl ether Sigma-Aldrich Cat. 108014 Pierce Streptavidin Magnetic Beads Thermo Scientific Cat. 88817 Urea Supelco Cat. 108487 Iodoacetamide Sigma-Aldrich Cat. I1149 Dithiothreitol (DTT) Fisher Scientific Cat. BP172-25 Trypsin, sequencing grade Promega Cat. V5111 Acetonitrile, LC/MS grade Fisher Scientific Cat. A955 (Continued on next page) Cell Reports 45, 117507, June 23, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Hydroxylamine solution Sigma-Aldrich Cat. 467804 EPPS Alfa Aesar Cat. A13714-22 TMTpro 16-plex Label Reagent Set Thermo Scientific Cat. A44521 Lipopolysaccharide (LPS) Sigma-Aldrich Cat. L2880 Nitrocellulose membrane, 0.2 μm Bio-Rad Cat. 1620112 Intercept (TBS) Blocking Buffer LI-COR Cat. 927-60001 D-(+)-Glucose Sigma-Aldrich Cat. G8270 Critical commercial assays DC assay kit Bio-Rad Cat. 5000111 BCA assay kit Thermo Scientific Cat. 23228 Pierce C18 pipette tips Thermo Scientific Cat. 87784 Sep-Pak Vac C18 cartridges Waters Cat. WAT054955 Glucose Meter, Glucose Strips Nova Max Plus Blood Glucose Monitoring System Cat. 8548043524 QuantiTect Reverse Transcription Kit QIAGEN Cat. 205311 SYBR GreenER qPCR SuperMix Invitrogen Cat. 11760500 Deposited data All raw proteomic data has been uploaded to PRIDE This paper PRIDE: PXD075539 Experimental Models: Organisms/strains TurboIDKDEL This paper Albumin-Cre (B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J) The Jackson Laboratory Strain 003574 Adiponectin-Cre (B6.FVB-Tg(Adipoq-cre) 1Evdr/J) The Jackson Laboratory Strain 028020 CD19-Cre (B6.129P2(C)-Cd19tm1(cre)Cgn/J) The Jackson Laboratory Strain 006785 Wild-type (C57BL/6J) The Jackson Laboratory Strain 000664 Recombinant DNA CTV plasmid Addgene Cat. 15912 Software and algorithms TurboID analysis pipeline This paper github.com/vinogradova-lab/ cell-type-specific-proximity-labeling IP2 and ProLuCID Integrated Proteomics Applications Xu et al., J Proteomics (2015)75 RawConverter (v1.2.0.1) Yates Lab, Scripps Research He et al., Anal Chem (2015)76 NumPy (v1.22.4) Van der Walt et al., 201077 numpy.org Pandas (v1.4.3) McKinney, 201078 pandas.pydata.org ggplot2 (v3.3.5) Wikham, 201679 ggplot2.tidyverse.org SignalP (v6.0) Emanuelsson, 200725 services.healthtech.dtu.dk/ services/SignalP-6.0/ ComplexHeatmap (v2.10.0) Gu, 202280 github.com/jokergoo/ ComplexHeatmap GOATOOLS (v1.3.1) Klopfenstein et al. 201881 github.com/tanghaibao/goatools scikit-learn (v1.0.2) Pedregosa et al., 201182 scikit-learn.org/stable ggrepel (v0.9.1) github.com/slowkow/ggrepel Image Lab Software (v6.1) Bio-Rad Other Rodent regular chow diet PicoLab Cat. 5053 Rodent diet with 60 kcal% Fat Research Diets Cat. D12492 Rodent water bottle nozzle, 30mm, short straight Vision Racks & Cages Type E Microvette EDTA capillary blood collection tubes Sarstedt Cat. 16.444.100 22 Cell Reports 45, 117507, June 23, 2026 ..

    Plasmid Preparation:

    Article Title: Cell-type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Antibodies Streptavidin, HRP-linked Cell Signaling Cat. 3999S AB_10830897 anti-V5 tag Abcam Cat. ab206562 AB_3719924 anti-Human IgG, HRP-linked Abcam Cat. ab6858 AB_955433 anti-mAcrp30 (adiponectin) R&D Systems Cat. AF1119 AB_2221770 anti-Goat IgG, HRP-linked Thermo Fisher Cat. A15999 AB_2534673 Streptavidin, Alexa 647 BioLegend Cat. 405237 anti-mAlbumin Cell Signaling Cat. 4929S AB_2225785 anti-Rabbit IgG, HRP-linked Cell Signaling Cat. 7074S AB_2099233 anti-BTK Cell Signaling Cat. 8547S AB_10950506 anti-Ces1d Santa Cruz Biotechnology Cat. sc-374160 AB_10988772 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR Cat. 926-32210 AB_621842 anti-Complement C6 Invitrogen Cat. PA5-117369 AB_2901999 IRDye 680CW Goat anti-Rabbit IgG Secondary LI-COR Cat. 926-68071 AB_10956166 anti-SerpinA3N R&D Systems Cat. AF4709 AB_2270116 IRDye 680RD Donkey anti-Goat IgG Secondary LI-COR Cat. 926-68074 AB_10956736 IRDye 800CW Streptavidin LI-COR Cat. 926-32230 anti-mFactor D (Adipsin, CFAD) R&D Systems Cat. AF5430 AB_1655868 anti-LRG1 Sigma-Aldrich Cat. HPA001888 AB_1079276 anti-FABP4 R&D Systems Cat. AF1443-SP AB_2102444 anti-mRetinol binding protein 4 R&D Systems Cat. AF3476 AB_2167682 anti-Sheep IgG, HRP-linked Thermo Fisher Cat. A16041 AB_2534715 anti-Vinculin Cell Signaling Cat. 4650S AB_10559207 Chemicals, peptides, and recombinant proteins Biotin Sigma-Aldrich Cat. B4501 NP-40 Millipore Cat. 492016 cOmplete Protease Inhibitor Cocktail tablets Roche Cat. 04693159001 PhosphoSTOP Roche Cat. 04906837001 XT MES Buffer Bio-Rad Cat. 1610789 Criterion 4–12% Bis-Tris 12-well gels Bio-Rad Cat. 3450123 Tris/Glycine transfer Buffer Bio-Rad Cat. 1610771 SuperBlock T20 blocking buffer Thermo Scientific Cat. 37536 0.45 μm PVDF membrane Millipore Cat. IPVH00010 Enhanced Chemiluminescence (ECL) PerkinElmer Cat. NEL104001EA Paraformaldehyde Sigma-Aldrich Cat. P6148 Dichloromethane Fisher Scientific Cat. AA39116K2 Hydrogen peroxide, 30% Fisher Scientific Cat. H325100 Heparin Sigma-Aldrich Cat. H3393 Dibenzyl ether Sigma-Aldrich Cat. 108014 Pierce Streptavidin Magnetic Beads Thermo Scientific Cat. 88817 Urea Supelco Cat. 108487 Iodoacetamide Sigma-Aldrich Cat. I1149 Dithiothreitol (DTT) Fisher Scientific Cat. BP172-25 Trypsin, sequencing grade Promega Cat. V5111 Acetonitrile, LC/MS grade Fisher Scientific Cat. A955 (Continued on next page) Cell Reports 45, 117507, June 23, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Hydroxylamine solution Sigma-Aldrich Cat. 467804 EPPS Alfa Aesar Cat. A13714-22 TMTpro 16-plex Label Reagent Set Thermo Scientific Cat. A44521 Lipopolysaccharide (LPS) Sigma-Aldrich Cat. L2880 Nitrocellulose membrane, 0.2 μm Bio-Rad Cat. 1620112 Intercept (TBS) Blocking Buffer LI-COR Cat. 927-60001 D-(+)-Glucose Sigma-Aldrich Cat. G8270 Critical commercial assays DC assay kit Bio-Rad Cat. 5000111 BCA assay kit Thermo Scientific Cat. 23228 Pierce C18 pipette tips Thermo Scientific Cat. 87784 Sep-Pak Vac C18 cartridges Waters Cat. WAT054955 Glucose Meter, Glucose Strips Nova Max Plus Blood Glucose Monitoring System Cat. 8548043524 QuantiTect Reverse Transcription Kit QIAGEN Cat. 205311 SYBR GreenER qPCR SuperMix Invitrogen Cat. 11760500 Deposited data All raw proteomic data has been uploaded to PRIDE This paper PRIDE: PXD075539 Experimental Models: Organisms/strains TurboIDKDEL This paper Albumin-Cre (B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J) The Jackson Laboratory Strain 003574 Adiponectin-Cre (B6.FVB-Tg(Adipoq-cre) 1Evdr/J) The Jackson Laboratory Strain 028020 CD19-Cre (B6.129P2(C)-Cd19tm1(cre)Cgn/J) The Jackson Laboratory Strain 006785 Wild-type (C57BL/6J) The Jackson Laboratory Strain 000664 Recombinant DNA CTV plasmid Addgene Cat. 15912 Software and algorithms TurboID analysis pipeline This paper github.com/vinogradova-lab/ cell-type-specific-proximity-labeling IP2 and ProLuCID Integrated Proteomics Applications Xu et al., J Proteomics (2015)75 RawConverter (v1.2.0.1) Yates Lab, Scripps Research He et al., Anal Chem (2015)76 NumPy (v1.22.4) Van der Walt et al., 201077 numpy.org Pandas (v1.4.3) McKinney, 201078 pandas.pydata.org ggplot2 (v3.3.5) Wikham, 201679 ggplot2.tidyverse.org SignalP (v6.0) Emanuelsson, 200725 services.healthtech.dtu.dk/ services/SignalP-6.0/ ComplexHeatmap (v2.10.0) Gu, 202280 github.com/jokergoo/ ComplexHeatmap GOATOOLS (v1.3.1) Klopfenstein et al. 201881 github.com/tanghaibao/goatools scikit-learn (v1.0.2) Pedregosa et al., 201182 scikit-learn.org/stable ggrepel (v0.9.1) github.com/slowkow/ggrepel Image Lab Software (v6.1) Bio-Rad Other Rodent regular chow diet PicoLab Cat. 5053 Rodent diet with 60 kcal% Fat Research Diets Cat. D12492 Rodent water bottle nozzle, 30mm, short straight Vision Racks & Cages Type E Microvette EDTA capillary blood collection tubes Sarstedt Cat. 16.444.100 22 Cell Reports 45, 117507, June 23, 2026 ..

    Software:

    Article Title: Cell-type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Antibodies Streptavidin, HRP-linked Cell Signaling Cat. 3999S AB_10830897 anti-V5 tag Abcam Cat. ab206562 AB_3719924 anti-Human IgG, HRP-linked Abcam Cat. ab6858 AB_955433 anti-mAcrp30 (adiponectin) R&D Systems Cat. AF1119 AB_2221770 anti-Goat IgG, HRP-linked Thermo Fisher Cat. A15999 AB_2534673 Streptavidin, Alexa 647 BioLegend Cat. 405237 anti-mAlbumin Cell Signaling Cat. 4929S AB_2225785 anti-Rabbit IgG, HRP-linked Cell Signaling Cat. 7074S AB_2099233 anti-BTK Cell Signaling Cat. 8547S AB_10950506 anti-Ces1d Santa Cruz Biotechnology Cat. sc-374160 AB_10988772 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR Cat. 926-32210 AB_621842 anti-Complement C6 Invitrogen Cat. PA5-117369 AB_2901999 IRDye 680CW Goat anti-Rabbit IgG Secondary LI-COR Cat. 926-68071 AB_10956166 anti-SerpinA3N R&D Systems Cat. AF4709 AB_2270116 IRDye 680RD Donkey anti-Goat IgG Secondary LI-COR Cat. 926-68074 AB_10956736 IRDye 800CW Streptavidin LI-COR Cat. 926-32230 anti-mFactor D (Adipsin, CFAD) R&D Systems Cat. AF5430 AB_1655868 anti-LRG1 Sigma-Aldrich Cat. HPA001888 AB_1079276 anti-FABP4 R&D Systems Cat. AF1443-SP AB_2102444 anti-mRetinol binding protein 4 R&D Systems Cat. AF3476 AB_2167682 anti-Sheep IgG, HRP-linked Thermo Fisher Cat. A16041 AB_2534715 anti-Vinculin Cell Signaling Cat. 4650S AB_10559207 Chemicals, peptides, and recombinant proteins Biotin Sigma-Aldrich Cat. B4501 NP-40 Millipore Cat. 492016 cOmplete Protease Inhibitor Cocktail tablets Roche Cat. 04693159001 PhosphoSTOP Roche Cat. 04906837001 XT MES Buffer Bio-Rad Cat. 1610789 Criterion 4–12% Bis-Tris 12-well gels Bio-Rad Cat. 3450123 Tris/Glycine transfer Buffer Bio-Rad Cat. 1610771 SuperBlock T20 blocking buffer Thermo Scientific Cat. 37536 0.45 μm PVDF membrane Millipore Cat. IPVH00010 Enhanced Chemiluminescence (ECL) PerkinElmer Cat. NEL104001EA Paraformaldehyde Sigma-Aldrich Cat. P6148 Dichloromethane Fisher Scientific Cat. AA39116K2 Hydrogen peroxide, 30% Fisher Scientific Cat. H325100 Heparin Sigma-Aldrich Cat. H3393 Dibenzyl ether Sigma-Aldrich Cat. 108014 Pierce Streptavidin Magnetic Beads Thermo Scientific Cat. 88817 Urea Supelco Cat. 108487 Iodoacetamide Sigma-Aldrich Cat. I1149 Dithiothreitol (DTT) Fisher Scientific Cat. BP172-25 Trypsin, sequencing grade Promega Cat. V5111 Acetonitrile, LC/MS grade Fisher Scientific Cat. A955 (Continued on next page) Cell Reports 45, 117507, June 23, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Hydroxylamine solution Sigma-Aldrich Cat. 467804 EPPS Alfa Aesar Cat. A13714-22 TMTpro 16-plex Label Reagent Set Thermo Scientific Cat. A44521 Lipopolysaccharide (LPS) Sigma-Aldrich Cat. L2880 Nitrocellulose membrane, 0.2 μm Bio-Rad Cat. 1620112 Intercept (TBS) Blocking Buffer LI-COR Cat. 927-60001 D-(+)-Glucose Sigma-Aldrich Cat. G8270 Critical commercial assays DC assay kit Bio-Rad Cat. 5000111 BCA assay kit Thermo Scientific Cat. 23228 Pierce C18 pipette tips Thermo Scientific Cat. 87784 Sep-Pak Vac C18 cartridges Waters Cat. WAT054955 Glucose Meter, Glucose Strips Nova Max Plus Blood Glucose Monitoring System Cat. 8548043524 QuantiTect Reverse Transcription Kit QIAGEN Cat. 205311 SYBR GreenER qPCR SuperMix Invitrogen Cat. 11760500 Deposited data All raw proteomic data has been uploaded to PRIDE This paper PRIDE: PXD075539 Experimental Models: Organisms/strains TurboIDKDEL This paper Albumin-Cre (B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J) The Jackson Laboratory Strain 003574 Adiponectin-Cre (B6.FVB-Tg(Adipoq-cre) 1Evdr/J) The Jackson Laboratory Strain 028020 CD19-Cre (B6.129P2(C)-Cd19tm1(cre)Cgn/J) The Jackson Laboratory Strain 006785 Wild-type (C57BL/6J) The Jackson Laboratory Strain 000664 Recombinant DNA CTV plasmid Addgene Cat. 15912 Software and algorithms TurboID analysis pipeline This paper github.com/vinogradova-lab/ cell-type-specific-proximity-labeling IP2 and ProLuCID Integrated Proteomics Applications Xu et al., J Proteomics (2015)75 RawConverter (v1.2.0.1) Yates Lab, Scripps Research He et al., Anal Chem (2015)76 NumPy (v1.22.4) Van der Walt et al., 201077 numpy.org Pandas (v1.4.3) McKinney, 201078 pandas.pydata.org ggplot2 (v3.3.5) Wikham, 201679 ggplot2.tidyverse.org SignalP (v6.0) Emanuelsson, 200725 services.healthtech.dtu.dk/ services/SignalP-6.0/ ComplexHeatmap (v2.10.0) Gu, 202280 github.com/jokergoo/ ComplexHeatmap GOATOOLS (v1.3.1) Klopfenstein et al. 201881 github.com/tanghaibao/goatools scikit-learn (v1.0.2) Pedregosa et al., 201182 scikit-learn.org/stable ggrepel (v0.9.1) github.com/slowkow/ggrepel Image Lab Software (v6.1) Bio-Rad Other Rodent regular chow diet PicoLab Cat. 5053 Rodent diet with 60 kcal% Fat Research Diets Cat. D12492 Rodent water bottle nozzle, 30mm, short straight Vision Racks & Cages Type E Microvette EDTA capillary blood collection tubes Sarstedt Cat. 16.444.100 22 Cell Reports 45, 117507, June 23, 2026 ..

    Fat:

    Article Title: Cell-type-specific proximity labeling of organ secretomes reveals energy balance-dependent proteomic remodeling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Antibodies Streptavidin, HRP-linked Cell Signaling Cat. 3999S AB_10830897 anti-V5 tag Abcam Cat. ab206562 AB_3719924 anti-Human IgG, HRP-linked Abcam Cat. ab6858 AB_955433 anti-mAcrp30 (adiponectin) R&D Systems Cat. AF1119 AB_2221770 anti-Goat IgG, HRP-linked Thermo Fisher Cat. A15999 AB_2534673 Streptavidin, Alexa 647 BioLegend Cat. 405237 anti-mAlbumin Cell Signaling Cat. 4929S AB_2225785 anti-Rabbit IgG, HRP-linked Cell Signaling Cat. 7074S AB_2099233 anti-BTK Cell Signaling Cat. 8547S AB_10950506 anti-Ces1d Santa Cruz Biotechnology Cat. sc-374160 AB_10988772 IRDye 800CW Goat anti-Mouse IgG Secondary LI-COR Cat. 926-32210 AB_621842 anti-Complement C6 Invitrogen Cat. PA5-117369 AB_2901999 IRDye 680CW Goat anti-Rabbit IgG Secondary LI-COR Cat. 926-68071 AB_10956166 anti-SerpinA3N R&D Systems Cat. AF4709 AB_2270116 IRDye 680RD Donkey anti-Goat IgG Secondary LI-COR Cat. 926-68074 AB_10956736 IRDye 800CW Streptavidin LI-COR Cat. 926-32230 anti-mFactor D (Adipsin, CFAD) R&D Systems Cat. AF5430 AB_1655868 anti-LRG1 Sigma-Aldrich Cat. HPA001888 AB_1079276 anti-FABP4 R&D Systems Cat. AF1443-SP AB_2102444 anti-mRetinol binding protein 4 R&D Systems Cat. AF3476 AB_2167682 anti-Sheep IgG, HRP-linked Thermo Fisher Cat. A16041 AB_2534715 anti-Vinculin Cell Signaling Cat. 4650S AB_10559207 Chemicals, peptides, and recombinant proteins Biotin Sigma-Aldrich Cat. B4501 NP-40 Millipore Cat. 492016 cOmplete Protease Inhibitor Cocktail tablets Roche Cat. 04693159001 PhosphoSTOP Roche Cat. 04906837001 XT MES Buffer Bio-Rad Cat. 1610789 Criterion 4–12% Bis-Tris 12-well gels Bio-Rad Cat. 3450123 Tris/Glycine transfer Buffer Bio-Rad Cat. 1610771 SuperBlock T20 blocking buffer Thermo Scientific Cat. 37536 0.45 μm PVDF membrane Millipore Cat. IPVH00010 Enhanced Chemiluminescence (ECL) PerkinElmer Cat. NEL104001EA Paraformaldehyde Sigma-Aldrich Cat. P6148 Dichloromethane Fisher Scientific Cat. AA39116K2 Hydrogen peroxide, 30% Fisher Scientific Cat. H325100 Heparin Sigma-Aldrich Cat. H3393 Dibenzyl ether Sigma-Aldrich Cat. 108014 Pierce Streptavidin Magnetic Beads Thermo Scientific Cat. 88817 Urea Supelco Cat. 108487 Iodoacetamide Sigma-Aldrich Cat. I1149 Dithiothreitol (DTT) Fisher Scientific Cat. BP172-25 Trypsin, sequencing grade Promega Cat. V5111 Acetonitrile, LC/MS grade Fisher Scientific Cat. A955 (Continued on next page) Cell Reports 45, 117507, June 23, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER/RRID Hydroxylamine solution Sigma-Aldrich Cat. 467804 EPPS Alfa Aesar Cat. A13714-22 TMTpro 16-plex Label Reagent Set Thermo Scientific Cat. A44521 Lipopolysaccharide (LPS) Sigma-Aldrich Cat. L2880 Nitrocellulose membrane, 0.2 μm Bio-Rad Cat. 1620112 Intercept (TBS) Blocking Buffer LI-COR Cat. 927-60001 D-(+)-Glucose Sigma-Aldrich Cat. G8270 Critical commercial assays DC assay kit Bio-Rad Cat. 5000111 BCA assay kit Thermo Scientific Cat. 23228 Pierce C18 pipette tips Thermo Scientific Cat. 87784 Sep-Pak Vac C18 cartridges Waters Cat. WAT054955 Glucose Meter, Glucose Strips Nova Max Plus Blood Glucose Monitoring System Cat. 8548043524 QuantiTect Reverse Transcription Kit QIAGEN Cat. 205311 SYBR GreenER qPCR SuperMix Invitrogen Cat. 11760500 Deposited data All raw proteomic data has been uploaded to PRIDE This paper PRIDE: PXD075539 Experimental Models: Organisms/strains TurboIDKDEL This paper Albumin-Cre (B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J) The Jackson Laboratory Strain 003574 Adiponectin-Cre (B6.FVB-Tg(Adipoq-cre) 1Evdr/J) The Jackson Laboratory Strain 028020 CD19-Cre (B6.129P2(C)-Cd19tm1(cre)Cgn/J) The Jackson Laboratory Strain 006785 Wild-type (C57BL/6J) The Jackson Laboratory Strain 000664 Recombinant DNA CTV plasmid Addgene Cat. 15912 Software and algorithms TurboID analysis pipeline This paper github.com/vinogradova-lab/ cell-type-specific-proximity-labeling IP2 and ProLuCID Integrated Proteomics Applications Xu et al., J Proteomics (2015)75 RawConverter (v1.2.0.1) Yates Lab, Scripps Research He et al., Anal Chem (2015)76 NumPy (v1.22.4) Van der Walt et al., 201077 numpy.org Pandas (v1.4.3) McKinney, 201078 pandas.pydata.org ggplot2 (v3.3.5) Wikham, 201679 ggplot2.tidyverse.org SignalP (v6.0) Emanuelsson, 200725 services.healthtech.dtu.dk/ services/SignalP-6.0/ ComplexHeatmap (v2.10.0) Gu, 202280 github.com/jokergoo/ ComplexHeatmap GOATOOLS (v1.3.1) Klopfenstein et al. 201881 github.com/tanghaibao/goatools scikit-learn (v1.0.2) Pedregosa et al., 201182 scikit-learn.org/stable ggrepel (v0.9.1) github.com/slowkow/ggrepel Image Lab Software (v6.1) Bio-Rad Other Rodent regular chow diet PicoLab Cat. 5053 Rodent diet with 60 kcal% Fat Research Diets Cat. D12492 Rodent water bottle nozzle, 30mm, short straight Vision Racks & Cages Type E Microvette EDTA capillary blood collection tubes Sarstedt Cat. 16.444.100 22 Cell Reports 45, 117507, June 23, 2026 ..



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    Sangon Biotech human srf sirna
    Role of <t>SRF</t> in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and <t>tamoxifen-inducible,</t> <t>adipocyte-specific</t> Srf KO <t>(SRF-AKO,</t> n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Human Srf Sirna, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/srf/2+5+accacgtt+antisense+auaa+fn1+gucc+human+sirna+ugauaca/pmc13167570-115-31-34
    Average 86 stars, based on 1 article reviews
    human srf sirna - by Bioz Stars, 2026-10
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    srf  (Proteintech)
    94
    Proteintech srf
    Role of <t>SRF</t> in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and <t>tamoxifen-inducible,</t> <t>adipocyte-specific</t> Srf KO <t>(SRF-AKO,</t> n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Srf, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/srf/MKL1+Antibody/pm41921762-139-9-10
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    srf - by Bioz Stars, 2026-10
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    hsrf plasmid  (OriGene)
    94
    OriGene hsrf plasmid
    Role of <t>SRF</t> in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and <t>tamoxifen-inducible,</t> <t>adipocyte-specific</t> Srf KO <t>(SRF-AKO,</t> n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Hsrf Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/srf/Serum+Response+Factor+(SRF)+(NM_001292001)+Human+Tagged+ORF+Clone/bio_rxiv__64898__2026__03__27__714707-355-1-6
    Average 94 stars, based on 1 article reviews
    hsrf plasmid - by Bioz Stars, 2026-10
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    anti srf  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc anti srf
    Role of <t>SRF</t> in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and <t>tamoxifen-inducible,</t> <t>adipocyte-specific</t> Srf KO <t>(SRF-AKO,</t> n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Anti Srf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/srf/pm41861925-89-9-11
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    Image Search Results


    Critical role of SRF in regulating actin cytoskeletal gene expression in adipocytes in vitro . (A) Motif identified by MEME that is enriched in H3K27ac peaks within the HFD-associated super-enhancer regions. (B) Alignment of the SRF binding motif identified de novo from SRF ChIP-Seq in TGFβ1-treated 3T3L1 adipocytes, compared to the canonical SRF motif from the HOMER database. (C) Pathway analysis of genes associated with SRF ChIP-seq peaks. (D) Genomic tracks showing SRF and H3K27ac ChIP-seq signals at the Acta2 locus, highlighting SRF binding induced by TGFβ1 treatment (indicated by a black arrow) within an adipocyte super-enhancer region. (E–G) In vitro loss- and gain-of function experiments in 3T3-L1 adipocytes. Gene expression analysis of cytoskeletal genes following (E) Srf knockdown ( shSrf , n = 3 per condition, total N = 6) and (F) overexpression ( Srf OE, n = 3 per condition, total N = 6). (G) Western blot analysis of SRF and ACTA2 protein levels upon Srf overexpression ( Srf OE, n = 2 per condition, total N = 4). A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Metabolism: clinical and experimental

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    doi: 10.1016/j.metabol.2026.156548

    Figure Lengend Snippet: Critical role of SRF in regulating actin cytoskeletal gene expression in adipocytes in vitro . (A) Motif identified by MEME that is enriched in H3K27ac peaks within the HFD-associated super-enhancer regions. (B) Alignment of the SRF binding motif identified de novo from SRF ChIP-Seq in TGFβ1-treated 3T3L1 adipocytes, compared to the canonical SRF motif from the HOMER database. (C) Pathway analysis of genes associated with SRF ChIP-seq peaks. (D) Genomic tracks showing SRF and H3K27ac ChIP-seq signals at the Acta2 locus, highlighting SRF binding induced by TGFβ1 treatment (indicated by a black arrow) within an adipocyte super-enhancer region. (E–G) In vitro loss- and gain-of function experiments in 3T3-L1 adipocytes. Gene expression analysis of cytoskeletal genes following (E) Srf knockdown ( shSrf , n = 3 per condition, total N = 6) and (F) overexpression ( Srf OE, n = 3 per condition, total N = 6). (G) Western blot analysis of SRF and ACTA2 protein levels upon Srf overexpression ( Srf OE, n = 2 per condition, total N = 4). A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: For generation of SRF knockout mice specific to beige and brown adipocytes (SRF-BKO), Srf -flox mice were crossed with Ucp1 -Cre mice (Jackson Laboratory, 024670).

    Techniques: Gene Expression, In Vitro, Binding Assay, ChIP-sequencing, Knockdown, Over Expression, Western Blot, Two Tailed Test

    Role of SRF in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and tamoxifen-inducible, adipocyte-specific Srf KO (SRF-AKO, n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Metabolism: clinical and experimental

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    doi: 10.1016/j.metabol.2026.156548

    Figure Lengend Snippet: Role of SRF in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and tamoxifen-inducible, adipocyte-specific Srf KO (SRF-AKO, n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: For generation of SRF knockout mice specific to beige and brown adipocytes (SRF-BKO), Srf -flox mice were crossed with Ucp1 -Cre mice (Jackson Laboratory, 024670).

    Techniques: In Vivo, Expressing, Staining, Isolation, Two Tailed Test

    Compromised structural integrity and increased cellular fragility in SRF-deficient adipocytes. (A) Basal and isoproterenol (ISO)-stimulated lipolysis, measured by glycerol release from 4-h eWAT and iWAT explants from WT and SRF-AKO male mice ( n = 3 per condition, total N = 12). (B) Representative transmission electron microscopy (TEM) images of eWAT from WT and SRF-AKO female mice, showing ruptured adipocyte membranes (indicated by black arrows). Scale bar: 5 μm. (C) Quantification of apoptotic cells in eWAT and iWAT from WT (eWAT, n = 3; iWAT, n = 5) and SRF-AKO (eWAT, n = 3; iWAT, n = 3) male mice by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. (D) BODIPY staining of eWAT and iWAT from WT and SRF-AKO male mice following 1.5-h of compression. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.

    Journal: Metabolism: clinical and experimental

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    doi: 10.1016/j.metabol.2026.156548

    Figure Lengend Snippet: Compromised structural integrity and increased cellular fragility in SRF-deficient adipocytes. (A) Basal and isoproterenol (ISO)-stimulated lipolysis, measured by glycerol release from 4-h eWAT and iWAT explants from WT and SRF-AKO male mice ( n = 3 per condition, total N = 12). (B) Representative transmission electron microscopy (TEM) images of eWAT from WT and SRF-AKO female mice, showing ruptured adipocyte membranes (indicated by black arrows). Scale bar: 5 μm. (C) Quantification of apoptotic cells in eWAT and iWAT from WT (eWAT, n = 3; iWAT, n = 5) and SRF-AKO (eWAT, n = 3; iWAT, n = 3) male mice by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. (D) BODIPY staining of eWAT and iWAT from WT and SRF-AKO male mice following 1.5-h of compression. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.

    Article Snippet: For generation of SRF knockout mice specific to beige and brown adipocytes (SRF-BKO), Srf -flox mice were crossed with Ucp1 -Cre mice (Jackson Laboratory, 024670).

    Techniques: Transmission Assay, Electron Microscopy, TUNEL Assay, Staining, Two Tailed Test

    Impaired vascular integrity and altered cell-cell communication in adipose tissue driven by loss of SRF in adipocytes. (A) UMAP visualization of 34,457 single nuclei isolated from eWAT and iWAT of WT and SRF-AKO male mice (total N = 4), with annotated cell types. (B) Violin plots of cell type-specific marker gene expression across all identified cell types. (C) Relative proportions of each cell type in eWAT and iWAT from WT and SRF-AKO male mice. (D–E) Whole-mount staining of eWAT from WT and SRF-AKO mice with Hoechst (blue), BODIPY (green), and either F4/80 (red, D) or PECAM1 (red, E). Scale bar: 100 μm. (F) Circle plots from CellChat analysis showing altered cell-cell communication in eWAT of SRF-AKO male mouse compared to WT. Increases in interaction number (left) and strength (right) are shown in red; decreases are shown in blue.

    Journal: Metabolism: clinical and experimental

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    doi: 10.1016/j.metabol.2026.156548

    Figure Lengend Snippet: Impaired vascular integrity and altered cell-cell communication in adipose tissue driven by loss of SRF in adipocytes. (A) UMAP visualization of 34,457 single nuclei isolated from eWAT and iWAT of WT and SRF-AKO male mice (total N = 4), with annotated cell types. (B) Violin plots of cell type-specific marker gene expression across all identified cell types. (C) Relative proportions of each cell type in eWAT and iWAT from WT and SRF-AKO male mice. (D–E) Whole-mount staining of eWAT from WT and SRF-AKO mice with Hoechst (blue), BODIPY (green), and either F4/80 (red, D) or PECAM1 (red, E). Scale bar: 100 μm. (F) Circle plots from CellChat analysis showing altered cell-cell communication in eWAT of SRF-AKO male mouse compared to WT. Increases in interaction number (left) and strength (right) are shown in red; decreases are shown in blue.

    Article Snippet: For generation of SRF knockout mice specific to beige and brown adipocytes (SRF-BKO), Srf -flox mice were crossed with Ucp1 -Cre mice (Jackson Laboratory, 024670).

    Techniques: Isolation, Marker, Gene Expression, Staining

    Role of SRF in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and tamoxifen-inducible, adipocyte-specific Srf KO (SRF-AKO, n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Metabolism: clinical and experimental

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    doi: 10.1016/j.metabol.2026.156548

    Figure Lengend Snippet: Role of SRF in actin filament structure and cellular expansion in adipocytes in vivo during obesity. (A) Body weight trajectories of wild-type (WT, n = 11) and tamoxifen-inducible, adipocyte-specific Srf KO (SRF-AKO, n = 9) male mice during HFD feeding. Total N = 20. (B) Heatmap showing the relative expression of cytoskeletal and collagen genes in eWAT from WT ( n = 6) and SRF-AKO male mice ( n = 5). Total N = 11. (C) Phalloidin staining of actin filaments in isolated adipocytes from WT and SRF-AKO male mice (scale bar: 20 μm). (D–E) Co-staining of isolated adipocytes with phalloidin (red) and PLIN1 (green) from both eWAT and iWAT of female mice. Scale bar: 100 μm, with quantification of phalloidin signal intensity. (F–G) Representative H&E-stained adipose tissue sections from (F) eWAT and (G) iWAT of WT and SRF-AKO male mice, with quantification of average adipocyte size. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: To generate inducible adipocyte-specific SRF knockout (SRF-AKO) mice, Srf -flox mice (Jackson Laboratory, 006658) were crossed with Adipoq -CreERT2 mice (Jackson Laboratory, 024671).

    Techniques: In Vivo, Expressing, Staining, Isolation, Two Tailed Test

    Impaired systemic glucose homeostasis and partial lipodystrophy phenotype in SRF-AKO mice during obesity. (A–C) Defective glucose homeostasis in SRF-AKO ( n = 9) compared to WT ( n = 11) male mice during obesity (total N = 20), assessed by (A) glucose tolerance test (GTT, 1 g/kg body weight glucose), (B) insulin tolerance test (ITT, 1 U/kg body weight insulin), and (C) fasting insulin levels (ng/mL) relative to body weight. (D) Tissue weight (% of body weight) of eWAT, iWAT, and BAT, and liver in WT ( n = 11) and SRF-AKO ( n = 9) male mice. Total N = 20. (E–F) Representative H&E-stained sections of (E) BAT and (F) liver from WT and SRF-AKO male mice. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.

    Journal: Metabolism: clinical and experimental

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    doi: 10.1016/j.metabol.2026.156548

    Figure Lengend Snippet: Impaired systemic glucose homeostasis and partial lipodystrophy phenotype in SRF-AKO mice during obesity. (A–C) Defective glucose homeostasis in SRF-AKO ( n = 9) compared to WT ( n = 11) male mice during obesity (total N = 20), assessed by (A) glucose tolerance test (GTT, 1 g/kg body weight glucose), (B) insulin tolerance test (ITT, 1 U/kg body weight insulin), and (C) fasting insulin levels (ng/mL) relative to body weight. (D) Tissue weight (% of body weight) of eWAT, iWAT, and BAT, and liver in WT ( n = 11) and SRF-AKO ( n = 9) male mice. Total N = 20. (E–F) Representative H&E-stained sections of (E) BAT and (F) liver from WT and SRF-AKO male mice. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.

    Article Snippet: To generate inducible adipocyte-specific SRF knockout (SRF-AKO) mice, Srf -flox mice (Jackson Laboratory, 006658) were crossed with Adipoq -CreERT2 mice (Jackson Laboratory, 024671).

    Techniques: Staining, Two Tailed Test

    Compromised structural integrity and increased cellular fragility in SRF-deficient adipocytes. (A) Basal and isoproterenol (ISO)-stimulated lipolysis, measured by glycerol release from 4-h eWAT and iWAT explants from WT and SRF-AKO male mice ( n = 3 per condition, total N = 12). (B) Representative transmission electron microscopy (TEM) images of eWAT from WT and SRF-AKO female mice, showing ruptured adipocyte membranes (indicated by black arrows). Scale bar: 5 μm. (C) Quantification of apoptotic cells in eWAT and iWAT from WT (eWAT, n = 3; iWAT, n = 5) and SRF-AKO (eWAT, n = 3; iWAT, n = 3) male mice by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. (D) BODIPY staining of eWAT and iWAT from WT and SRF-AKO male mice following 1.5-h of compression. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.

    Journal: Metabolism: clinical and experimental

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    doi: 10.1016/j.metabol.2026.156548

    Figure Lengend Snippet: Compromised structural integrity and increased cellular fragility in SRF-deficient adipocytes. (A) Basal and isoproterenol (ISO)-stimulated lipolysis, measured by glycerol release from 4-h eWAT and iWAT explants from WT and SRF-AKO male mice ( n = 3 per condition, total N = 12). (B) Representative transmission electron microscopy (TEM) images of eWAT from WT and SRF-AKO female mice, showing ruptured adipocyte membranes (indicated by black arrows). Scale bar: 5 μm. (C) Quantification of apoptotic cells in eWAT and iWAT from WT (eWAT, n = 3; iWAT, n = 5) and SRF-AKO (eWAT, n = 3; iWAT, n = 3) male mice by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. (D) BODIPY staining of eWAT and iWAT from WT and SRF-AKO male mice following 1.5-h of compression. Scale bar: 100 μm. A two-tailed Student’s t -test was used for statistical analysis. * P < 0.05, ** P < 0.01.

    Article Snippet: To generate inducible adipocyte-specific SRF knockout (SRF-AKO) mice, Srf -flox mice (Jackson Laboratory, 006658) were crossed with Adipoq -CreERT2 mice (Jackson Laboratory, 024671).

    Techniques: Transmission Assay, Electron Microscopy, TUNEL Assay, Staining, Two Tailed Test

    Impaired vascular integrity and altered cell-cell communication in adipose tissue driven by loss of SRF in adipocytes. (A) UMAP visualization of 34,457 single nuclei isolated from eWAT and iWAT of WT and SRF-AKO male mice (total N = 4), with annotated cell types. (B) Violin plots of cell type-specific marker gene expression across all identified cell types. (C) Relative proportions of each cell type in eWAT and iWAT from WT and SRF-AKO male mice. (D–E) Whole-mount staining of eWAT from WT and SRF-AKO mice with Hoechst (blue), BODIPY (green), and either F4/80 (red, D) or PECAM1 (red, E). Scale bar: 100 μm. (F) Circle plots from CellChat analysis showing altered cell-cell communication in eWAT of SRF-AKO male mouse compared to WT. Increases in interaction number (left) and strength (right) are shown in red; decreases are shown in blue.

    Journal: Metabolism: clinical and experimental

    Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice

    doi: 10.1016/j.metabol.2026.156548

    Figure Lengend Snippet: Impaired vascular integrity and altered cell-cell communication in adipose tissue driven by loss of SRF in adipocytes. (A) UMAP visualization of 34,457 single nuclei isolated from eWAT and iWAT of WT and SRF-AKO male mice (total N = 4), with annotated cell types. (B) Violin plots of cell type-specific marker gene expression across all identified cell types. (C) Relative proportions of each cell type in eWAT and iWAT from WT and SRF-AKO male mice. (D–E) Whole-mount staining of eWAT from WT and SRF-AKO mice with Hoechst (blue), BODIPY (green), and either F4/80 (red, D) or PECAM1 (red, E). Scale bar: 100 μm. (F) Circle plots from CellChat analysis showing altered cell-cell communication in eWAT of SRF-AKO male mouse compared to WT. Increases in interaction number (left) and strength (right) are shown in red; decreases are shown in blue.

    Article Snippet: To generate inducible adipocyte-specific SRF knockout (SRF-AKO) mice, Srf -flox mice (Jackson Laboratory, 006658) were crossed with Adipoq -CreERT2 mice (Jackson Laboratory, 024671).

    Techniques: Isolation, Marker, Gene Expression, Staining