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spots  (Rockland Immunochemicals)


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    Structured Review

    Rockland Immunochemicals spots
    Spots, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spotting+solution+for+microarray+printing/Ponceau+S+solution/us11891436-339-15-47
    Average 94 stars, based on 97 article reviews
    spots - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: A Salmonella type I toxin promotes systemic infection by inhibiting F o F 1 ATP synthase
    Article Snippet: .. The eluates were resolved on 12% SDS-polyacrylamide gels, transferred to a nitrocellulose membrane, and analyzed by Western blot using anti-HA (1:5,000 dilution, Rockland) and anti-GFP (1:5,000 dilution, Santa Cruz) antibodies overnight. .. The blots were developed by incubation with anti-mouse IgG horseradish peroxidase-linked antibodies (1:10,000 dilution, ThermoFisher) or anti-rabbit IgG horseradish peroxidase-linked antibodies (1:10,000 dilution, ThermoFisher) for 1 h and were detected using the ECL detection system (SuperSignal® West Femto Maximum Sensitivity Substrate, ThermoFisher).

    Article Title: Exploring the Role of Kv1.3 and MAPK14 in Mediating Microglial Oxidative Stress and Neuroinflammation Following Organic Dust Exposure
    Article Snippet: Equal amounts of proteins (20 μg/well) were resolved on 10% SDS-PAGE gels (Bio-Rad). .. Next, proteins were transferred to a nitrocellulose membrane, and the nonspecific binding sites were blocked for an hour with a blocking buffer specially formulated for fluorescent western blotting (Rockland Immunochemicals, Pottstown, PA). ..

    Article Title: UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemia.
    Article Snippet: Bio-Rad precast 4–12% Bis-Tris gels were used to separate proteins, at a voltage of 150 V for 60min. .. Gels were transferred onto a nitrocellulose membrane through wet transfer at 30 V for 70 min. Membranes were blocked in Rockland blocking buffer (#MB070; Rockland Immunochemicals) for 1 h at room temperature. .. Primary antibodies were diluted in Rockland blocking buffer and incubated overnight at 4°C.

    Article Title: Highly effective antifibrotic compound discovered as a contaminant in cefixime preparations: preliminary characterization
    Article Snippet: .. For western blot, the samples were resolved by 7.5% SDS-PAGE, transferred onto nitrocellulose membrane and probed with anti-COL1A1 antibody (Rockland). .. The cells in 12-well plates were fixed with 3.7% formaldehyde/0.05% glutaraldehyde/0.5% Triton X-100, blocked with 10% BSA and incubated with 1:10 dilution of anti-collagen antibody (Rockland) overnight.

    Article Title: UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemia
    Article Snippet: A prestained protein ladder was used to determine molecular weight (Cat #26616; Thermo Fisher Scientific). .. Bio-Rad precast 4–12% Bis-Tris gels were used to separate proteins, at a voltage of 150 V for 60 min. Gels were transferred onto a nitrocellulose membrane through wet transfer at 30 V for 70 min. Membranes were blocked in Rockland blocking buffer (#MB-070; Rockland Immunochemicals) for 1 h at room temperature. .. Primary antibodies were diluted in Rockland blocking buffer and incubated overnight at 4°C.

    Western Blot:

    Article Title: A Salmonella type I toxin promotes systemic infection by inhibiting F o F 1 ATP synthase
    Article Snippet: .. The eluates were resolved on 12% SDS-polyacrylamide gels, transferred to a nitrocellulose membrane, and analyzed by Western blot using anti-HA (1:5,000 dilution, Rockland) and anti-GFP (1:5,000 dilution, Santa Cruz) antibodies overnight. .. The blots were developed by incubation with anti-mouse IgG horseradish peroxidase-linked antibodies (1:10,000 dilution, ThermoFisher) or anti-rabbit IgG horseradish peroxidase-linked antibodies (1:10,000 dilution, ThermoFisher) for 1 h and were detected using the ECL detection system (SuperSignal® West Femto Maximum Sensitivity Substrate, ThermoFisher).

    Article Title: Exploring the Role of Kv1.3 and MAPK14 in Mediating Microglial Oxidative Stress and Neuroinflammation Following Organic Dust Exposure
    Article Snippet: Equal amounts of proteins (20 μg/well) were resolved on 10% SDS-PAGE gels (Bio-Rad). .. Next, proteins were transferred to a nitrocellulose membrane, and the nonspecific binding sites were blocked for an hour with a blocking buffer specially formulated for fluorescent western blotting (Rockland Immunochemicals, Pottstown, PA). ..

    Article Title: Highly effective antifibrotic compound discovered as a contaminant in cefixime preparations: preliminary characterization
    Article Snippet: .. For western blot, the samples were resolved by 7.5% SDS-PAGE, transferred onto nitrocellulose membrane and probed with anti-COL1A1 antibody (Rockland). .. The cells in 12-well plates were fixed with 3.7% formaldehyde/0.05% glutaraldehyde/0.5% Triton X-100, blocked with 10% BSA and incubated with 1:10 dilution of anti-collagen antibody (Rockland) overnight.

    Binding Assay:

    Article Title: Exploring the Role of Kv1.3 and MAPK14 in Mediating Microglial Oxidative Stress and Neuroinflammation Following Organic Dust Exposure
    Article Snippet: Equal amounts of proteins (20 μg/well) were resolved on 10% SDS-PAGE gels (Bio-Rad). .. Next, proteins were transferred to a nitrocellulose membrane, and the nonspecific binding sites were blocked for an hour with a blocking buffer specially formulated for fluorescent western blotting (Rockland Immunochemicals, Pottstown, PA). ..

    Blocking Assay:

    Article Title: Exploring the Role of Kv1.3 and MAPK14 in Mediating Microglial Oxidative Stress and Neuroinflammation Following Organic Dust Exposure
    Article Snippet: Equal amounts of proteins (20 μg/well) were resolved on 10% SDS-PAGE gels (Bio-Rad). .. Next, proteins were transferred to a nitrocellulose membrane, and the nonspecific binding sites were blocked for an hour with a blocking buffer specially formulated for fluorescent western blotting (Rockland Immunochemicals, Pottstown, PA). ..

    Article Title: UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemia.
    Article Snippet: Bio-Rad precast 4–12% Bis-Tris gels were used to separate proteins, at a voltage of 150 V for 60min. .. Gels were transferred onto a nitrocellulose membrane through wet transfer at 30 V for 70 min. Membranes were blocked in Rockland blocking buffer (#MB070; Rockland Immunochemicals) for 1 h at room temperature. .. Primary antibodies were diluted in Rockland blocking buffer and incubated overnight at 4°C.

    Article Title: UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemia
    Article Snippet: A prestained protein ladder was used to determine molecular weight (Cat #26616; Thermo Fisher Scientific). .. Bio-Rad precast 4–12% Bis-Tris gels were used to separate proteins, at a voltage of 150 V for 60 min. Gels were transferred onto a nitrocellulose membrane through wet transfer at 30 V for 70 min. Membranes were blocked in Rockland blocking buffer (#MB-070; Rockland Immunochemicals) for 1 h at room temperature. .. Primary antibodies were diluted in Rockland blocking buffer and incubated overnight at 4°C.

    other:

    Article Title: TERT Immunohistochemistry in Thin Melanomas Compared to Melanocytic Nevi
    Article Snippet: Afterwards, we performed the immunohistochemical staining using the Anti-Telomerase Catalytic Subunit antibody—600-401-252 (rabbit) produced by Rockland in Limerick, Pennsylvania, USA, as they used in the study by Hugdahl et al. [ ].

    Immunofluorescence:

    Article Title: KRASG12D cells override homeostatic cell elimination mechanisms in adult pancreas via Wnt5a and cell dormancy.
    Article Snippet: Sections were dehydrated and mounted with DPX (Sigma-Aldrich). .. 149 Immunofluorescence (IF) co-staining using either anti-CD44 and anti-RFP (Rockland) was 150 performed in FFPE tissues slices using the same antigen retrieval and primary antibody 151 incubations as described for IHC, followed by Alexa fluor secondary antibody incubation for 152 Jo urn al Pr e-p roo f 6 60 minutes at room temperature and Hoechst (ThermoFisher) (Supplementary table S14) and 153 mounted using Mowiol (Sigma-Aldrich). ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: KRASG12D cells override homeostatic cell elimination mechanisms in adult pancreas via Wnt5a and cell dormancy.
    Article Snippet: Sections were dehydrated and mounted with DPX (Sigma-Aldrich). .. 149 Immunofluorescence (IF) co-staining using either anti-CD44 and anti-RFP (Rockland) was 150 performed in FFPE tissues slices using the same antigen retrieval and primary antibody 151 incubations as described for IHC, followed by Alexa fluor secondary antibody incubation for 152 Jo urn al Pr e-p roo f 6 60 minutes at room temperature and Hoechst (ThermoFisher) (Supplementary table S14) and 153 mounted using Mowiol (Sigma-Aldrich). ..

    Immunohistochemistry:

    Article Title: KRASG12D cells override homeostatic cell elimination mechanisms in adult pancreas via Wnt5a and cell dormancy.
    Article Snippet: Sections were dehydrated and mounted with DPX (Sigma-Aldrich). .. 149 Immunofluorescence (IF) co-staining using either anti-CD44 and anti-RFP (Rockland) was 150 performed in FFPE tissues slices using the same antigen retrieval and primary antibody 151 incubations as described for IHC, followed by Alexa fluor secondary antibody incubation for 152 Jo urn al Pr e-p roo f 6 60 minutes at room temperature and Hoechst (ThermoFisher) (Supplementary table S14) and 153 mounted using Mowiol (Sigma-Aldrich). ..

    Incubation:

    Article Title: KRASG12D cells override homeostatic cell elimination mechanisms in adult pancreas via Wnt5a and cell dormancy.
    Article Snippet: Sections were dehydrated and mounted with DPX (Sigma-Aldrich). .. 149 Immunofluorescence (IF) co-staining using either anti-CD44 and anti-RFP (Rockland) was 150 performed in FFPE tissues slices using the same antigen retrieval and primary antibody 151 incubations as described for IHC, followed by Alexa fluor secondary antibody incubation for 152 Jo urn al Pr e-p roo f 6 60 minutes at room temperature and Hoechst (ThermoFisher) (Supplementary table S14) and 153 mounted using Mowiol (Sigma-Aldrich). ..

    Staining:

    Article Title: Anatomical, subset, and HIV-dependent expression of viral sensors and restriction factors
    Article Snippet: .. Cisplatin staining and fixation of cells for CyTOF analysis was performed as previously described., , , , Briefly, up to 6 million cells were resuspended in 2 mL contaminant-free PBS (Rockland) with 2 mM EDTA (Corning). .. Cells were then incubated for 1 min with an additional 2 mL of PBS/EDTA supplemented with 12.5 μM cisplatin (Sigma-Aldrich).



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