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imaris spot function  (Oxford Instruments)


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    Structured Review

    Oxford Instruments imaris spot function
    Imaris Spot Function, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spots+function/Imaris/pm41998164-95-7-7
    Average 99 stars, based on 44293 article reviews
    imaris spot function - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Analysis:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Morphology:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Electron Microscopy:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Software:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Fluorescence:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Confocal:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Transmission Electron Microscopy:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Oligomer Restriction:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Imaging:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Live Cell Imaging:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Microscopy:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect

    Patch Clamp:

    Article Title: Glial Dysfunction and Memory Impairments in a Model of Pediatric Obstructive Sleep Apnea.
    Article Snippet: e measure function. Experimenters were blinded to sample identity during image acquisition and analysis to ensure rigor and reproducibility. Astrocyte and oligodendrocyte morphology was examined using Imaris (v. 10) (Cheng et al. 2023 ). Individual cells were reconstructed using the filament tool, applying identical settings for processing each sample. Morphological measures such as Sholl intersect



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    Oxford Instruments imaris spot tool function
    (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant <t>tool</t> are highlighted in magenta. Scale bar: 30µm. (B) <t>Imaris</t> surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).
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      Buy from Supplier

    Image Search Results


    (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant tool are highlighted in magenta. Scale bar: 30µm. (B) Imaris surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).

    Journal: bioRxiv

    Article Title: Astrocyte targeted SMN1 gene therapy and forskolin application improves astrocyte filopodia actin defects and motor neuron synaptic dysfunction in human SMA disease pathology

    doi: 10.64898/2026.03.26.714618

    Figure Lengend Snippet: (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant tool are highlighted in magenta. Scale bar: 30µm. (B) Imaris surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).

    Article Snippet: Using the Imaris spot tool function, we quantified the CD44 and pERM puncta within astrocyte filopodia ( ) which revealed a statistically significant decrease in pERM , CD44 , and co-localized CD44/pERM ( ) expression within SMA patient-derived astrocytes cultures compared to healthy astrocytes at baseline and after actin remodeling stimulation.

    Techniques: Comparison