spin column based method (Qiagen)

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Qiagen spin column based method
Spin Column Based Method, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spin+column+based+method/QIAamp+Circulating+Nucleic+Acid+Kit/pmc12652532-240-8-17
Average 99 stars, based on 4560 article reviews

spin column based method - by Bioz Stars, 2026-10

99/100 stars

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Sensitivity:

Article Title: Cell-Free DNA as a Biomarker of Cell Death and Treatment Response for On-Chip Three-Dimensional Tumor Models.
Article Snippet: able S2 summarizes the primers used for amplifying DNA of 16 fragments containing human or mouse LINE regions (generated by Invitrogen, ThermoFisher Scientific, Ontario, Canada). .. Media frompatient-derivedMDTs also underwentDNAextractionusing QIAamp Circulating Nucleic Acid Kit (55114, Qiagen, Germantown, MD, USA) upon thawing to increase the assay’s sensitivity. .. Genomic DNA from Human (11691112001 for spheroids and cell line-derived MDTs and D4642 for patient-derived MDTs, MilliporeSigma,ON,Canada) andmouse (69239,MilliporeSigma) were used to build a standard curve of DNA content.

Article Title: Shotgun metagenomic sequencing of serum extracellular vesicle-derived DNA enables microbial profiling in urothelial carcinoma
Article Snippet: .. DNA isolation from serum EVs samples was conducted using the QIAamp Circulating Nucleic Acid Kit (QIAGEN, Hilden, Germany) following the manufacturer’s protocol and measured using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific). ..

DNA Extraction:

Qubit:

Clinical Proteomics:

Article Title: Longitudinal changes in circulating HPV16 DNA during chemoradiotherapy and their association with tumor burden in oropharyngeal cancer
Article Snippet: .. Cell free DNA (cfDNA) was extracted from 2 mL plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat.55114, Hilden, Germany) according to the manufacturer’s instructions. .. The concentration of cfDNA was measuared using Qubit dsDNA Quantification Assay Kit (Invitrogen, Cat. Q32854, MA, USA) on Qubit 3 Fluorometer (Invitrogen, Cat.Q33216, MA, USA).

Article Title: Early experience with cerebrospinal fluid cell-free DNA molecular profiling in a neuro-oncology practice
Article Snippet: .. All CSF cfDNA extraction and sequencing was performed at Predicine, Inc. cfDNA was isolated from CSF using the QIAamp Circulating Nucleic Acid Kit (Qiagen, catalog 55114). .. Purified cfDNA was assessed for quantity and quality on a Qubit fluorometer (Thermo Fisher Scientific) and a Fragment Analyzer (Agilent Technologies).

Article Title: Early plasma cell-free DNA and monocyte human leukocyte antigen-DR expression for predicting 28-day mortality in sepsis: A single-center prospective cohort study with internal validation.
Article Snippet: .. For each patient, cfDNA was extracted from 1.0 mL of plasma using the QIAamp Circulating Nucleic Acid Kit (QIAGEN, Germany) according to the manufacturer’s instructions, with a final elution volume of 50 μL. ..

Article Title: Clinical outcomes and blood-based biomarkers across two SABR-treated metastatic settings: oligoprogression under immune checkpoint inhibition and oligometastatic disease
Article Snippet: .. cfDNA was isolated from plasma with the QIAamp Circulating Nucleic Acid kit (Qiagen, Germantown, MD, 55114, USA) according to the manufacturer’s protocol. .. cfDNA was measured using 1X Qubit High Sensitivity instrument (Thermo Fisher Scientific, Waltham, MA, USA).

Extraction:

Sequencing:

Isolation:

Article Title: Disrupted neuronal expression of autism risk genes and white matter micro-organization after infant Zika infection.
Article Snippet: 450 ZIKV-RNA detection by qRT-PCR: Total RNA was extracted from 140 μl of plasma using the 451 QIAamp Viral RNA Mini Kit (Qiagen). .. RNA from urine was isolated using the QIAamp 452 Circulating Nucleic Acid Kit (Qiagen). .. ZIKV-RNA standard was generated by annealing two 453 oligonucleotides spanning target ZIKV prM-E gene region and performing in vitro transcription 454 using the MEGAscript T3 Transcription Kit (Ambion).

Modification:

Article Title: Urine cell-free RNA for bladder cancer detection and treatment response prediction.
Article Snippet: .. Cell-free nucleic acids were extracted from urine using the miRNA protocol from the QIAamp Circulating Nucleic Acid kit (Qiagen (range 1–20 ml)) with slight modification. ..

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