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data acquisition and analysis program spike 2 version 4.2  (Cambridge Electronic Design)

 
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    Structured Review

    Cambridge Electronic Design data acquisition and analysis program spike 2 version 4.2
    Data Acquisition And Analysis Program Spike 2 Version 4.2, supplied by Cambridge Electronic Design, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spike+analysis+programs/spike2+software/pmc06386380-164-8-17
    Average 90 stars, based on 1 article reviews
    data acquisition and analysis program spike 2 version 4.2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Software:

    Article Title: Altered Brain Energy Metabolism in the APPPS1 Alzheimer’s Model during anesthesia: Integration of Experimental Data and In Silico Modeling
    Article Snippet: GFAP- and IBA1-covered area and TREM2 intensity were analyzed using ImageJ. .. Analog signals were digitalized using the Power CED1401 and Spike2 software (both from Cambridge Electronic Design, Cambridge, UK). .. Data analysis and statistical evaluations were conducted with Spike2, Excel (Microsoft, Seattle, USA), MATLAB (MathWorks Inc., Natick, USA), and R Statistics (R Core Team, Vienna, Austria).

    Article Title: Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms
    Article Snippet: .. The acquired EEG/EMG signal was filtered between 1 and 500 Hz using the A-M System (model 1800) with an additional notch filter at 60 Hz, simultaneously digitized at a rate of 1 kHz, and stored on a computer via CED Micro1401-3 interface and Spike2, version 9, software (Cambridge Electronic Design). ..

    Article Title: Differentiation of Hebbian and homeostatic plasticity mechanisms within layer 5 visual cortex neurons
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57Bl/6J wild type mice Charles River, UK RRID: IMSR_JAX:000664 transgenic homozygous aCaMKIIT286A mutant mice A gift from Silva lab at UCLA http://www.silvalab.org/index.html aCaMKIIT286A Chemicals, peptides, and recombinant proteins XPro1595 INmune Bio Inc, USA XPro1595 6/0 non-absorbable sutures Mersilk-Ethicon, J&J W529H 1.0% Chloramphenicol 1.0% w/w Eye ointment Martindale Pharma 1.0% Chloramphenicol 1.0% w/w Eye ointment Red RetroBeads Lumafluor inc., NC, USA Red RetrobeadsTM Meloxicam Boehringer Ingelheim Metacam Dexamethasone Northbrook Laboratories, UK Colvasone 0.2% w/v Lidocaine Hydrochloride injection 1% Hamlen, UK Lidocaine Hydrochloride Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Baxter Healthcare Ltd, UK Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Clark Borosilicate glass capillaries Warner instruments, USA GC100F-10 UMP3 UltraMicro Pump for cortical injections World precision instruments UMP3-4 CED Micro 1401 AD-converter Cambridge Electronic Design Limited, UK Micro1401 Multiclamp 700B amplifier Molecular devices Multiclamp 700B Biocytin Merck 576-19-2 Sodium chloride Merck S7653 Potassium chloride Fisher scientific P/4280/53 Calcium chloride Merck 21115 Magnesium sulfate Merck 434183 Sodium Bicarbonate Fisher scientific BP328-500 Sodium phosphate monobasic Merck S0751 Potassium D-gluconate Merck P4500 HEPES Merck H3375 Adenosine 50-triphosphate magnesium salt (ATP Magnesium salt) Merck A9187 Guanosine 50-triphosphate sodium salt hydrate (GTP Sodium salt) Merck G8877 D(+)-Glucose, ACS reagent, anhydrous Thermo Fisher scientific 410950010 Choline chloride Merck C7527 Tetrodotoxin citrate Hello Bio, UK HB1035 DL-AP5 Hello Bio, UK HB0251 Picrotoxin Hello Bio, UK HB0506 Paraformaldehyde Merck 8.18715 Alexa Fluor 488 Streptavidin Invitrogen S11223 Software and algorithms Signal Cambridge Electronic Design Limited, UK Signal version 7 Spike2 Cambridge Electronic Design Limited, UK Spike2 version 4 AxoGraph Axograph.com AxoGraph 1.7.6 Matlab Mathworks Matlab R2021b Matlab graphing tools for plotting CDF from mEPSC amplitude and inter-event interval files Github https://doi.org/10.5281/ zenodo.6482880 ImageJ NIH, USA ImageJ JMP SAS software, USA JMP Cell Reports 39, 110892, May 31, 2022 e1 Article ll OPEN ACCESS ..

    Article Title: Nicotine engages a VTA-NAc feedback loop to inhibit amygdala-projecting dopamine neurons and induce anxiety-like behaviors.
    Article Snippet: Electrical signals were amplified by a high-impedance amplifier (Axon Instruments) and monitored through an audio monitor (A.M. Systems Inc.). .. The unit activity was digitized at 25 kHz and recorded using Spike2 software (Cambridge Electronic Design) for later analysis. ..

    Article Title: The selective amyloid-driven failure of cholinergic medial septal neurons in aging mice perturbs REM sleep, cognition and emotion, and broadcasts amyloid to other brain regions
    Article Snippet: .. The demodulated signal was digitized using a CED 1401 Micro Box (Cambridge Electronic Design) and recorded at a sampling rate of 1 kHz using Spike2 software (Cambridge Electronic Design). .. All photometry data were analyzed using MATLAB.

    Article Title: Activation of serotonergic system with fluoxetine or 5-HTP restores blunted hypercapnic ventilatory response in Parkinson’s disease model
    Article Snippet: The recorded activities of the PHR were amplified and filtered (5–2500 Hz) using a NeuroLog system (Digitimer Ltd., Welwyn, UK) and integrated with the time constant of 70 ms. .. Raw and integrated nervous activity and arterial blood pressure were digitized using a CED Power 1401 data acquisition interface, recorded on a computer, and analyzed using the Spike 2 software (Cambridge Electronic Design, Cambridge, UK). .. Baseline nerve activity was accomplished by manipulating inhaled CO 2 and respiratory pump rate and/or volume, while PETCO 2 levels were monitored until phrenic nerve activity achieved a stable level.

    Transgenic Assay:

    Article Title: Differentiation of Hebbian and homeostatic plasticity mechanisms within layer 5 visual cortex neurons
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57Bl/6J wild type mice Charles River, UK RRID: IMSR_JAX:000664 transgenic homozygous aCaMKIIT286A mutant mice A gift from Silva lab at UCLA http://www.silvalab.org/index.html aCaMKIIT286A Chemicals, peptides, and recombinant proteins XPro1595 INmune Bio Inc, USA XPro1595 6/0 non-absorbable sutures Mersilk-Ethicon, J&J W529H 1.0% Chloramphenicol 1.0% w/w Eye ointment Martindale Pharma 1.0% Chloramphenicol 1.0% w/w Eye ointment Red RetroBeads Lumafluor inc., NC, USA Red RetrobeadsTM Meloxicam Boehringer Ingelheim Metacam Dexamethasone Northbrook Laboratories, UK Colvasone 0.2% w/v Lidocaine Hydrochloride injection 1% Hamlen, UK Lidocaine Hydrochloride Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Baxter Healthcare Ltd, UK Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Clark Borosilicate glass capillaries Warner instruments, USA GC100F-10 UMP3 UltraMicro Pump for cortical injections World precision instruments UMP3-4 CED Micro 1401 AD-converter Cambridge Electronic Design Limited, UK Micro1401 Multiclamp 700B amplifier Molecular devices Multiclamp 700B Biocytin Merck 576-19-2 Sodium chloride Merck S7653 Potassium chloride Fisher scientific P/4280/53 Calcium chloride Merck 21115 Magnesium sulfate Merck 434183 Sodium Bicarbonate Fisher scientific BP328-500 Sodium phosphate monobasic Merck S0751 Potassium D-gluconate Merck P4500 HEPES Merck H3375 Adenosine 50-triphosphate magnesium salt (ATP Magnesium salt) Merck A9187 Guanosine 50-triphosphate sodium salt hydrate (GTP Sodium salt) Merck G8877 D(+)-Glucose, ACS reagent, anhydrous Thermo Fisher scientific 410950010 Choline chloride Merck C7527 Tetrodotoxin citrate Hello Bio, UK HB1035 DL-AP5 Hello Bio, UK HB0251 Picrotoxin Hello Bio, UK HB0506 Paraformaldehyde Merck 8.18715 Alexa Fluor 488 Streptavidin Invitrogen S11223 Software and algorithms Signal Cambridge Electronic Design Limited, UK Signal version 7 Spike2 Cambridge Electronic Design Limited, UK Spike2 version 4 AxoGraph Axograph.com AxoGraph 1.7.6 Matlab Mathworks Matlab R2021b Matlab graphing tools for plotting CDF from mEPSC amplitude and inter-event interval files Github https://doi.org/10.5281/ zenodo.6482880 ImageJ NIH, USA ImageJ JMP SAS software, USA JMP Cell Reports 39, 110892, May 31, 2022 e1 Article ll OPEN ACCESS ..

    Mutagenesis:

    Article Title: Differentiation of Hebbian and homeostatic plasticity mechanisms within layer 5 visual cortex neurons
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57Bl/6J wild type mice Charles River, UK RRID: IMSR_JAX:000664 transgenic homozygous aCaMKIIT286A mutant mice A gift from Silva lab at UCLA http://www.silvalab.org/index.html aCaMKIIT286A Chemicals, peptides, and recombinant proteins XPro1595 INmune Bio Inc, USA XPro1595 6/0 non-absorbable sutures Mersilk-Ethicon, J&J W529H 1.0% Chloramphenicol 1.0% w/w Eye ointment Martindale Pharma 1.0% Chloramphenicol 1.0% w/w Eye ointment Red RetroBeads Lumafluor inc., NC, USA Red RetrobeadsTM Meloxicam Boehringer Ingelheim Metacam Dexamethasone Northbrook Laboratories, UK Colvasone 0.2% w/v Lidocaine Hydrochloride injection 1% Hamlen, UK Lidocaine Hydrochloride Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Baxter Healthcare Ltd, UK Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Clark Borosilicate glass capillaries Warner instruments, USA GC100F-10 UMP3 UltraMicro Pump for cortical injections World precision instruments UMP3-4 CED Micro 1401 AD-converter Cambridge Electronic Design Limited, UK Micro1401 Multiclamp 700B amplifier Molecular devices Multiclamp 700B Biocytin Merck 576-19-2 Sodium chloride Merck S7653 Potassium chloride Fisher scientific P/4280/53 Calcium chloride Merck 21115 Magnesium sulfate Merck 434183 Sodium Bicarbonate Fisher scientific BP328-500 Sodium phosphate monobasic Merck S0751 Potassium D-gluconate Merck P4500 HEPES Merck H3375 Adenosine 50-triphosphate magnesium salt (ATP Magnesium salt) Merck A9187 Guanosine 50-triphosphate sodium salt hydrate (GTP Sodium salt) Merck G8877 D(+)-Glucose, ACS reagent, anhydrous Thermo Fisher scientific 410950010 Choline chloride Merck C7527 Tetrodotoxin citrate Hello Bio, UK HB1035 DL-AP5 Hello Bio, UK HB0251 Picrotoxin Hello Bio, UK HB0506 Paraformaldehyde Merck 8.18715 Alexa Fluor 488 Streptavidin Invitrogen S11223 Software and algorithms Signal Cambridge Electronic Design Limited, UK Signal version 7 Spike2 Cambridge Electronic Design Limited, UK Spike2 version 4 AxoGraph Axograph.com AxoGraph 1.7.6 Matlab Mathworks Matlab R2021b Matlab graphing tools for plotting CDF from mEPSC amplitude and inter-event interval files Github https://doi.org/10.5281/ zenodo.6482880 ImageJ NIH, USA ImageJ JMP SAS software, USA JMP Cell Reports 39, 110892, May 31, 2022 e1 Article ll OPEN ACCESS ..

    Recombinant:

    Article Title: Differentiation of Hebbian and homeostatic plasticity mechanisms within layer 5 visual cortex neurons
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57Bl/6J wild type mice Charles River, UK RRID: IMSR_JAX:000664 transgenic homozygous aCaMKIIT286A mutant mice A gift from Silva lab at UCLA http://www.silvalab.org/index.html aCaMKIIT286A Chemicals, peptides, and recombinant proteins XPro1595 INmune Bio Inc, USA XPro1595 6/0 non-absorbable sutures Mersilk-Ethicon, J&J W529H 1.0% Chloramphenicol 1.0% w/w Eye ointment Martindale Pharma 1.0% Chloramphenicol 1.0% w/w Eye ointment Red RetroBeads Lumafluor inc., NC, USA Red RetrobeadsTM Meloxicam Boehringer Ingelheim Metacam Dexamethasone Northbrook Laboratories, UK Colvasone 0.2% w/v Lidocaine Hydrochloride injection 1% Hamlen, UK Lidocaine Hydrochloride Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Baxter Healthcare Ltd, UK Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Clark Borosilicate glass capillaries Warner instruments, USA GC100F-10 UMP3 UltraMicro Pump for cortical injections World precision instruments UMP3-4 CED Micro 1401 AD-converter Cambridge Electronic Design Limited, UK Micro1401 Multiclamp 700B amplifier Molecular devices Multiclamp 700B Biocytin Merck 576-19-2 Sodium chloride Merck S7653 Potassium chloride Fisher scientific P/4280/53 Calcium chloride Merck 21115 Magnesium sulfate Merck 434183 Sodium Bicarbonate Fisher scientific BP328-500 Sodium phosphate monobasic Merck S0751 Potassium D-gluconate Merck P4500 HEPES Merck H3375 Adenosine 50-triphosphate magnesium salt (ATP Magnesium salt) Merck A9187 Guanosine 50-triphosphate sodium salt hydrate (GTP Sodium salt) Merck G8877 D(+)-Glucose, ACS reagent, anhydrous Thermo Fisher scientific 410950010 Choline chloride Merck C7527 Tetrodotoxin citrate Hello Bio, UK HB1035 DL-AP5 Hello Bio, UK HB0251 Picrotoxin Hello Bio, UK HB0506 Paraformaldehyde Merck 8.18715 Alexa Fluor 488 Streptavidin Invitrogen S11223 Software and algorithms Signal Cambridge Electronic Design Limited, UK Signal version 7 Spike2 Cambridge Electronic Design Limited, UK Spike2 version 4 AxoGraph Axograph.com AxoGraph 1.7.6 Matlab Mathworks Matlab R2021b Matlab graphing tools for plotting CDF from mEPSC amplitude and inter-event interval files Github https://doi.org/10.5281/ zenodo.6482880 ImageJ NIH, USA ImageJ JMP SAS software, USA JMP Cell Reports 39, 110892, May 31, 2022 e1 Article ll OPEN ACCESS ..

    Ointment:

    Article Title: Differentiation of Hebbian and homeostatic plasticity mechanisms within layer 5 visual cortex neurons
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57Bl/6J wild type mice Charles River, UK RRID: IMSR_JAX:000664 transgenic homozygous aCaMKIIT286A mutant mice A gift from Silva lab at UCLA http://www.silvalab.org/index.html aCaMKIIT286A Chemicals, peptides, and recombinant proteins XPro1595 INmune Bio Inc, USA XPro1595 6/0 non-absorbable sutures Mersilk-Ethicon, J&J W529H 1.0% Chloramphenicol 1.0% w/w Eye ointment Martindale Pharma 1.0% Chloramphenicol 1.0% w/w Eye ointment Red RetroBeads Lumafluor inc., NC, USA Red RetrobeadsTM Meloxicam Boehringer Ingelheim Metacam Dexamethasone Northbrook Laboratories, UK Colvasone 0.2% w/v Lidocaine Hydrochloride injection 1% Hamlen, UK Lidocaine Hydrochloride Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Baxter Healthcare Ltd, UK Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Clark Borosilicate glass capillaries Warner instruments, USA GC100F-10 UMP3 UltraMicro Pump for cortical injections World precision instruments UMP3-4 CED Micro 1401 AD-converter Cambridge Electronic Design Limited, UK Micro1401 Multiclamp 700B amplifier Molecular devices Multiclamp 700B Biocytin Merck 576-19-2 Sodium chloride Merck S7653 Potassium chloride Fisher scientific P/4280/53 Calcium chloride Merck 21115 Magnesium sulfate Merck 434183 Sodium Bicarbonate Fisher scientific BP328-500 Sodium phosphate monobasic Merck S0751 Potassium D-gluconate Merck P4500 HEPES Merck H3375 Adenosine 50-triphosphate magnesium salt (ATP Magnesium salt) Merck A9187 Guanosine 50-triphosphate sodium salt hydrate (GTP Sodium salt) Merck G8877 D(+)-Glucose, ACS reagent, anhydrous Thermo Fisher scientific 410950010 Choline chloride Merck C7527 Tetrodotoxin citrate Hello Bio, UK HB1035 DL-AP5 Hello Bio, UK HB0251 Picrotoxin Hello Bio, UK HB0506 Paraformaldehyde Merck 8.18715 Alexa Fluor 488 Streptavidin Invitrogen S11223 Software and algorithms Signal Cambridge Electronic Design Limited, UK Signal version 7 Spike2 Cambridge Electronic Design Limited, UK Spike2 version 4 AxoGraph Axograph.com AxoGraph 1.7.6 Matlab Mathworks Matlab R2021b Matlab graphing tools for plotting CDF from mEPSC amplitude and inter-event interval files Github https://doi.org/10.5281/ zenodo.6482880 ImageJ NIH, USA ImageJ JMP SAS software, USA JMP Cell Reports 39, 110892, May 31, 2022 e1 Article ll OPEN ACCESS ..

    Injection:

    Article Title: Differentiation of Hebbian and homeostatic plasticity mechanisms within layer 5 visual cortex neurons
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57Bl/6J wild type mice Charles River, UK RRID: IMSR_JAX:000664 transgenic homozygous aCaMKIIT286A mutant mice A gift from Silva lab at UCLA http://www.silvalab.org/index.html aCaMKIIT286A Chemicals, peptides, and recombinant proteins XPro1595 INmune Bio Inc, USA XPro1595 6/0 non-absorbable sutures Mersilk-Ethicon, J&J W529H 1.0% Chloramphenicol 1.0% w/w Eye ointment Martindale Pharma 1.0% Chloramphenicol 1.0% w/w Eye ointment Red RetroBeads Lumafluor inc., NC, USA Red RetrobeadsTM Meloxicam Boehringer Ingelheim Metacam Dexamethasone Northbrook Laboratories, UK Colvasone 0.2% w/v Lidocaine Hydrochloride injection 1% Hamlen, UK Lidocaine Hydrochloride Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Baxter Healthcare Ltd, UK Sodium Chloride 0.9% w/v and Glucose 5% w/v Solution Clark Borosilicate glass capillaries Warner instruments, USA GC100F-10 UMP3 UltraMicro Pump for cortical injections World precision instruments UMP3-4 CED Micro 1401 AD-converter Cambridge Electronic Design Limited, UK Micro1401 Multiclamp 700B amplifier Molecular devices Multiclamp 700B Biocytin Merck 576-19-2 Sodium chloride Merck S7653 Potassium chloride Fisher scientific P/4280/53 Calcium chloride Merck 21115 Magnesium sulfate Merck 434183 Sodium Bicarbonate Fisher scientific BP328-500 Sodium phosphate monobasic Merck S0751 Potassium D-gluconate Merck P4500 HEPES Merck H3375 Adenosine 50-triphosphate magnesium salt (ATP Magnesium salt) Merck A9187 Guanosine 50-triphosphate sodium salt hydrate (GTP Sodium salt) Merck G8877 D(+)-Glucose, ACS reagent, anhydrous Thermo Fisher scientific 410950010 Choline chloride Merck C7527 Tetrodotoxin citrate Hello Bio, UK HB1035 DL-AP5 Hello Bio, UK HB0251 Picrotoxin Hello Bio, UK HB0506 Paraformaldehyde Merck 8.18715 Alexa Fluor 488 Streptavidin Invitrogen S11223 Software and algorithms Signal Cambridge Electronic Design Limited, UK Signal version 7 Spike2 Cambridge Electronic Design Limited, UK Spike2 version 4 AxoGraph Axograph.com AxoGraph 1.7.6 Matlab Mathworks Matlab R2021b Matlab graphing tools for plotting CDF from mEPSC amplitude and inter-event interval files Github https://doi.org/10.5281/ zenodo.6482880 ImageJ NIH, USA ImageJ JMP SAS software, USA JMP Cell Reports 39, 110892, May 31, 2022 e1 Article ll OPEN ACCESS ..

    Activity Assay:

    Article Title: Nicotine engages a VTA-NAc feedback loop to inhibit amygdala-projecting dopamine neurons and induce anxiety-like behaviors.
    Article Snippet: Electrical signals were amplified by a high-impedance amplifier (Axon Instruments) and monitored through an audio monitor (A.M. Systems Inc.). .. The unit activity was digitized at 25 kHz and recorded using Spike2 software (Cambridge Electronic Design) for later analysis. ..

    Article Title: Activation of serotonergic system with fluoxetine or 5-HTP restores blunted hypercapnic ventilatory response in Parkinson’s disease model
    Article Snippet: The recorded activities of the PHR were amplified and filtered (5–2500 Hz) using a NeuroLog system (Digitimer Ltd., Welwyn, UK) and integrated with the time constant of 70 ms. .. Raw and integrated nervous activity and arterial blood pressure were digitized using a CED Power 1401 data acquisition interface, recorded on a computer, and analyzed using the Spike 2 software (Cambridge Electronic Design, Cambridge, UK). .. Baseline nerve activity was accomplished by manipulating inhaled CO 2 and respiratory pump rate and/or volume, while PETCO 2 levels were monitored until phrenic nerve activity achieved a stable level.

    other:

    Article Title: Supporting Information for Adult neurogenesis is necessary for functional regeneration of a forebrain neural circuit
    Article Snippet: Action potentials were detected using Spike2.5 (Cambridge Electronic Design Limited) or pClamp software using simple threshold-based spike detection since all recordings had greater than 3:1 signal-to-noise ratio, and firing rate was calculated as the number of action potentials detected divided by the duration of the recording.

    Sampling:

    Article Title: The selective amyloid-driven failure of cholinergic medial septal neurons in aging mice perturbs REM sleep, cognition and emotion, and broadcasts amyloid to other brain regions
    Article Snippet: .. The demodulated signal was digitized using a CED 1401 Micro Box (Cambridge Electronic Design) and recorded at a sampling rate of 1 kHz using Spike2 software (Cambridge Electronic Design). .. All photometry data were analyzed using MATLAB.



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