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Gambro BCT Inc continuous-flow cobe spectra cell separator
Continuous Flow Cobe Spectra Cell Separator, supplied by Gambro BCT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spectral+flow-cytometry+data+preprocessing/cobe+spectra+cell+separator/pmc05654741-31-5-10
Average 90 stars, based on 1 article reviews
continuous-flow cobe spectra cell separator - by Bioz Stars, 2026-10
90/100 stars

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Isolation:

Article Title: Local intramuscular transplantation of autologous bone marrow mononuclear cells for critical lower limb ischaemia
Article Snippet: .. 8 centres used a bloodcell separator (COBE Spectra, version 4, Bone Marrow Processing Program, Gambro BCT, Lakewood, CO, USA); 2 centres used a blood-cell separator requiring a Ficoll density-gradient for isolation of the BMMNC.• dose of cells: 1.3 × 109 total (not clear in report)• route and frequency of delivery: IM injections (30 intramuscular injections of 1 mL) Control group: 30 mL saline with 4 mL autologous peripheral blood Outcomes Primary outcomes: • major amputation rate• mortality Secondary outcomes: • ABI• TcPO2• ulcers• pain Time points for assessment: baseline, days 1, 3, 15, 28, and then months 6 and 12 Funding The reported work was supported by the French Ministry of Health (Programme Hospitalier de Recherche Clinique 2007) and by the French Blood Agency. ..

Article Title: In vivo efficacy of endothelial growth medium stimulated mesenchymal stem cells derived from patients with critical limb ischemia
Article Snippet: .. BMCs were isolated using a blood cell separator (Cobe Spectra, version 4, BM Processing Program, Gambro BCT, Lakewood, CO, USA) and concentrated to obtain a final volume of 40 mL (30 mL for autologous injection and 10 mL for control analyses and cryopreservation). ..

Control:

Article Title: Local intramuscular transplantation of autologous bone marrow mononuclear cells for critical lower limb ischaemia
Article Snippet: .. 8 centres used a bloodcell separator (COBE Spectra, version 4, Bone Marrow Processing Program, Gambro BCT, Lakewood, CO, USA); 2 centres used a blood-cell separator requiring a Ficoll density-gradient for isolation of the BMMNC.• dose of cells: 1.3 × 109 total (not clear in report)• route and frequency of delivery: IM injections (30 intramuscular injections of 1 mL) Control group: 30 mL saline with 4 mL autologous peripheral blood Outcomes Primary outcomes: • major amputation rate• mortality Secondary outcomes: • ABI• TcPO2• ulcers• pain Time points for assessment: baseline, days 1, 3, 15, 28, and then months 6 and 12 Funding The reported work was supported by the French Ministry of Health (Programme Hospitalier de Recherche Clinique 2007) and by the French Blood Agency. ..

Article Title: In vivo efficacy of endothelial growth medium stimulated mesenchymal stem cells derived from patients with critical limb ischemia
Article Snippet: .. BMCs were isolated using a blood cell separator (Cobe Spectra, version 4, BM Processing Program, Gambro BCT, Lakewood, CO, USA) and concentrated to obtain a final volume of 40 mL (30 mL for autologous injection and 10 mL for control analyses and cryopreservation). ..

Saline:

Article Title: Local intramuscular transplantation of autologous bone marrow mononuclear cells for critical lower limb ischaemia
Article Snippet: .. 8 centres used a bloodcell separator (COBE Spectra, version 4, Bone Marrow Processing Program, Gambro BCT, Lakewood, CO, USA); 2 centres used a blood-cell separator requiring a Ficoll density-gradient for isolation of the BMMNC.• dose of cells: 1.3 × 109 total (not clear in report)• route and frequency of delivery: IM injections (30 intramuscular injections of 1 mL) Control group: 30 mL saline with 4 mL autologous peripheral blood Outcomes Primary outcomes: • major amputation rate• mortality Secondary outcomes: • ABI• TcPO2• ulcers• pain Time points for assessment: baseline, days 1, 3, 15, 28, and then months 6 and 12 Funding The reported work was supported by the French Ministry of Health (Programme Hospitalier de Recherche Clinique 2007) and by the French Blood Agency. ..

other:

Article Title: Local intramuscular transplantation of autologous bone marrow mononuclear cells for critical lower limb ischaemia
Article Snippet: In Pignon 2017, two diCerent approaches were performed for BMMNC separation across centres, including blood-cell separator using COBE Spectra, version 4, Bone Marrow Processing Program (Gambro BCT, Lakewood, CO, USA), and blood-cell separator requiring a Ficoll density-gradient for isolation of the BMMNC (COBE 2991, Gambro BCT).

Article Title: The clinical application of SNP-based next-generation sequencing (SNP-NGS) for evaluation of chimerism and microchimerism after HLA-mismatched stem cell microtransplantation.
Article Snippet: Apheresis of donor peripheral mononuclear cells was performed using a COBE SPECTRA cell separator (Gambro BCT, Lakewood, CA) and aliquoted into three parts.

Injection:

Article Title: In vivo efficacy of endothelial growth medium stimulated mesenchymal stem cells derived from patients with critical limb ischemia
Article Snippet: .. BMCs were isolated using a blood cell separator (Cobe Spectra, version 4, BM Processing Program, Gambro BCT, Lakewood, CO, USA) and concentrated to obtain a final volume of 40 mL (30 mL for autologous injection and 10 mL for control analyses and cryopreservation). ..



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