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1x spectral dapi  (Akoya Biosciences)


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    Structured Review

    Akoya Biosciences 1x spectral dapi
    1x Spectral Dapi, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 1317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spectral+dapi/Opal+480/pm41997939-503-13-16
    Average 99 stars, based on 1317 article reviews
    1x spectral dapi - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: mGlu 7 expression is upregulated by Tcf4 deficiency in a model of Pitt Hopkins syndrome.
    Article Snippet: A mouse Grm7 RNAscope probe (ACD Cat no. 487841-C3, Accession number: NM_177328.3) was then added for 2h at 40◦C for hybridization signal amplification. .. For detection, sections were incubated with OpalDye 570 (Akoya Biosciences Cat no. SKU FP1488001KT) diluted in TSA Buffer (ACD Cat no. 322810) at 1:500 for 30 min at 40◦C and DAPI (ACD) for counterstained nuclei. .. Confocal images were acquired using an LSM 880 laser-scanning confocal microscope (Carl Zeiss) equipped with a PlanApochromat 20×/0.8 NA air objective.

    Article Title: Multi-omics profiling of the choroid plexus reveals developmental cellular remodeling, early immune gene activation, and epithelial cell heterogeneity.
    Article Snippet: .. Opal fluorophores from Akoya Biosciences (Opal 520, PN FP1487001KT; Opal 570, PN FP1488001KT; Opal 690, PN FP1497001KT) were diluted 1:1500 in TSA buffer (322809) and incubated for 30min at 40◦C followed by 2 washes of 2min and HRP blocker incubation for 30min at 40◦C. ..



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    Image Search Results


    Example images of immunofluorescent staining on a pancreatic cancer core sample from a tissue microarray from different antibodies showing pan-cytokeratin (red, Opal 570, top left), SMAD4 (green, Opal 620, top right), and an unnamed biomarker (yellow, Opal 520, bottom left). The merged images are shown in the bottom right. Nuclei were stained blue with DAPI. Total cellular fluorescence intensity units of SMAD4 per cell in the pan-cytokeratin positive cells were determined by a Vectra Polaris microscope and Inform software.

    Journal: International journal of radiation oncology, biology, physics

    Article Title: High SMAD4 Expression is Associated with Improved Clinical Outcomes in Patients with Resectable Pancreatic Cancer: An Analysis of NRG Oncology/RTOG 9704

    doi: 10.1016/j.ijrobp.2026.09.014

    Figure Lengend Snippet: Example images of immunofluorescent staining on a pancreatic cancer core sample from a tissue microarray from different antibodies showing pan-cytokeratin (red, Opal 570, top left), SMAD4 (green, Opal 620, top right), and an unnamed biomarker (yellow, Opal 520, bottom left). The merged images are shown in the bottom right. Nuclei were stained blue with DAPI. Total cellular fluorescence intensity units of SMAD4 per cell in the pan-cytokeratin positive cells were determined by a Vectra Polaris microscope and Inform software.

    Article Snippet: Slides were counterstained with Spectral DAPI (Akoya Biosciences Cat. # FP1490) at a 1:100 dilution for 8 minutes.

    Techniques: Staining, Tissue, Microarray, Biomarker Discovery, Fluorescence, Microscopy, Software