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Becker & Hickl spcimage
Lifetime analysis using <t>SPCImage</t> software (Becker & Hickl). This example highlights analysis of the donor mTurquoise2 in the absence of acceptor using a mono-exponential fit, which allowed us to measure the lifetime of the donor in our cell line.
Spcimage, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spcimage+flim+data+analysis+software/spcimage/pmc08006575-91-6-10
Average 90 stars, based on 1 article reviews
spcimage - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "Live-cell FLIM-FRET using a commercially available system."

Article Title: Live-cell FLIM-FRET using a commercially available system.

Journal: Methods in cell biology

doi: 10.1016/bs.mcb.2020.02.002

Lifetime analysis using SPCImage software (Becker & Hickl). This example highlights analysis of the donor mTurquoise2 in the absence of acceptor using a mono-exponential fit, which allowed us to measure the lifetime of the donor in our cell line.
Figure Legend Snippet: Lifetime analysis using SPCImage software (Becker & Hickl). This example highlights analysis of the donor mTurquoise2 in the absence of acceptor using a mono-exponential fit, which allowed us to measure the lifetime of the donor in our cell line.

Techniques Used: Software

Related Articles

Produced:

Article Title: Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM
Article Snippet: .. The raw data captured and processed by the SPCImage (Becker & Hickl GmbH) were produced in 8-bit TIFF files using a custom MATLAB script to reduce storage requirements and speed up data read, write, and transfer . .. The raw data captured and processed by the SPCImage (Becker & Hickl GmbH) were produced in 8-bit TIFF files using a custom MATLAB script to reduce storage requirements and speed up data read, write, and transfer .

Software:

Article Title: Deep learning model utilizing fluorescence lifetime imaging microscopy and confidence learning for predicting endometrial cancer risk
Article Snippet: This study introduces a novel diagnostic approach for the prediction of endometrial cancer risk, which involves minimally invasive exfoliation cytology specimens from 63 participants, staining with the viscosity-sensitive fluorescent probe DCVJ, and detection by fluorescence lifetime imaging microscopy (FLIM).. Initially, DCVJstained tissue sections from cancerous and normal regions were utilized to confirm the elevated viscosity in cancerous lesions.. Subsequently, DCVJ-stained aspirated cell samples were detected by FLIM to capture viscosity characteristics and cellular morphology.

Article Title: Endonuclease G promotes hepatic mitochondrial respiration by selectively increasing mitochondrial tRNA Thr production.
Article Snippet: .. Fluorescence lifetime decay images were analyzed using the software SPCImage (Becker & Hickl GmbH, Berlin, Germany). ..

Microscopy:

Article Title: Deep learning model utilizing fluorescence lifetime imaging microscopy and confidence learning for predicting endometrial cancer risk
Article Snippet: This study introduces a novel diagnostic approach for the prediction of endometrial cancer risk, which involves minimally invasive exfoliation cytology specimens from 63 participants, staining with the viscosity-sensitive fluorescent probe DCVJ, and detection by fluorescence lifetime imaging microscopy (FLIM).. Initially, DCVJstained tissue sections from cancerous and normal regions were utilized to confirm the elevated viscosity in cancerous lesions.. Subsequently, DCVJ-stained aspirated cell samples were detected by FLIM to capture viscosity characteristics and cellular morphology.

Fluorescence:

Article Title: Endonuclease G promotes hepatic mitochondrial respiration by selectively increasing mitochondrial tRNA Thr production.
Article Snippet: .. Fluorescence lifetime decay images were analyzed using the software SPCImage (Becker & Hickl GmbH, Berlin, Germany). ..

other:

Article Title: Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating features of native capillary network.
Article Snippet: Fluorescence lifetime decays of NAD(P)H and FAD were deconvolved from the IRF and fit to a two-component exponential decay (SPCImage; Becker & Hickl) to quantify the lifetimes of free and protein-bound NAD(P)H and FAD [71, 72].

Article Title: A three-dimensional valve-on-chip microphysiological system implicates cell cycle progression, cholesterol metabolism and protein homeostasis in early calcific aortic valve disease progression.
Article Snippet: Background: Calcific aortic valve disease (CAVD) is one of the most common forms of valvulopathy, with a 50 % elevated risk of a fatal cardiovascular event, and greater than 15,0 0 0 annual deaths in North America alone.. The treatment standard is valve replacement as early diagnostic, mitigation, and drug strategies remain underdeveloped.. The development of early diagnostic and therapeutic strategies requires the fabrication of effective in vitro valve mimetic models to elucidate early CAVD mechanisms.



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Becker & Hickl spcimage flim data analysis software
Spcimage Flim Data Analysis Software, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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