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Becker & Hickl spcimage 5.2 software
Spcimage 5.2 Software, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spcimage+flim+data+analysis+software/spcimage+software/pm30448212-146-8-5
Average 90 stars, based on 1 article reviews
spcimage 5.2 software - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Multifunctional cyanoaryl porphyrazine pigments with push-pull structure of macrocycle framing: Photophysics and possible applications
Article Snippet: We report the experimental study of fluorescence properties of three porphyrazine derivatives, distinct in the number of fused aromatic rings in aryl groups contained in the macrocycle frame and/or presence of Pd in the macrocycle, in solutions with biological molecules and in methanol-glycerol mixtures.. Spectral and lifetime fluorescence analysis has revealed that all three pigments demonstrated two fluorescence transition bands from the first S1 and the second S2 electronic excited states to the ground state S0, and thus followed the anti-Kasha’s rule.. Strong dependence of the quantum yield and lifetimes in the S1 → S0 fluorescence band on solution viscosity was observed.

Software:

Article Title: Multifunctional cyanoaryl porphyrazine pigments with push-pull structure of macrocycle framing: Photophysics and possible applications
Article Snippet: We report the experimental study of fluorescence properties of three porphyrazine derivatives, distinct in the number of fused aromatic rings in aryl groups contained in the macrocycle frame and/or presence of Pd in the macrocycle, in solutions with biological molecules and in methanol-glycerol mixtures.. Spectral and lifetime fluorescence analysis has revealed that all three pigments demonstrated two fluorescence transition bands from the first S1 and the second S2 electronic excited states to the ground state S0, and thus followed the anti-Kasha’s rule.. Strong dependence of the quantum yield and lifetimes in the S1 → S0 fluorescence band on solution viscosity was observed.

other:

Article Title: Differentiation of Tumors of the Upper Respiratory Tract Using Optical Metabolic Imaging.
Article Snippet: FLIM data sets were analyzed in SPCImage 8.5 (Becker & Hickl GmbH, Berlin, Germany).

Article Title: Deep learning model utilizing fluorescence lifetime imaging microscopy and confidence learning for predicting endometrial cancer risk
Article Snippet: This study introduces a novel diagnostic approach for the prediction of endometrial cancer risk, which involves minimally invasive exfoliation cytology specimens from 63 participants, staining with the viscosity-sensitive fluorescent probe DCVJ, and detection by fluorescence lifetime imaging microscopy (FLIM).. Initially, DCVJstained tissue sections from cancerous and normal regions were utilized to confirm the elevated viscosity in cancerous lesions.. Subsequently, DCVJ-stained aspirated cell samples were detected by FLIM to capture viscosity characteristics and cellular morphology.

Article Title: Time-resolved fluorescence of ANS dye as a sensor of proteins LLPS.
Article Snippet: The explosive growth in the number of works addressing the phase separation of intrinsically disordered proteins has driven both the development of new approaches and the optimization of existing methods for biomolecular condensate visualization.. In this work, we studied the potential use of the fluorescent dye ANS as a sensor for liquid-liquid phase separation (LLPS), focusing on visualizing condensates formed by the stress-granules scaffold protein G3BP1.. Using fluorescence lifetime imaging microscopy (FLIM), we demonstrated that ANS can accumulate in RNA-induced G3BP1 condensates in aqueous solutions, but not in G3BP1 condensates formed under macromolecular crowding conditions in highly concentrated PEG solutions.

Article Title: Gold Nanoparticle Detection with Two-Photon Excitation Fluorescence Lifetime Imaging of NAD(P)H in Cancer Cells: An Analytical Approach to Separate Nanoparticle and NAD(P)H Signals.
Article Snippet: Gold nanoparticles (AuNPs) have shown promise for applications in the diagnosis and treatment of different diseases, including cancer.. Understanding the effect of AuNPs on metabolic reprogramming in cancer cells at the single cell level is of high importance for improving the efficacy and safety.. Fluorescence lifetime imaging microscopy (FLIM) of nicotinamide adenine dinucleotide (phosphate) hydrogen (NAD(P)H) as a main metabolic cofactor and an indicator of metabolic reprogramming in cancer cells enables real-time monitoring of cancer cell metabolism in response to different treatments, including AuNPs.

Article Title: Assessing Wound Healing in Vivo Using a Dual-Function Phosphorescent Probe Sensitive to Tissue Oxygenation and Regenerating Collagen
Article Snippet: Data analysis was performed using SPCImage 8.4 software (Becker & Hickl).

Article Title: Cellular damage triggers mechano-chemical control of cell wall dynamics and patterned cell divisions in plant healing.
Article Snippet: For root stainedwithmechano-probes, after image acquisition, SPCImage 8.5 software (Becker &Hickl) was used to select and bin ROIs and quantified the data.



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Becker & Hickl spcimage flim data analysis software
Spcimage Flim Data Analysis Software, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spcimage+flim+data+analysis+software/spc+image+analysis+software/pm21256962-200-8-14
Average 90 stars, based on 1 article reviews
spcimage flim data analysis software - by Bioz Stars, 2026-11
90/100 stars
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