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grna coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc grna coding sequence
    Grna Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spcas9+hf1+coding+sequence/pX459V2%2E0-SpCas9-HF1+(Plasmid+%23108293)/pm34883448-10-162-166
    Average 93 stars, based on 4 article reviews
    grna coding sequence - by Bioz Stars, 2026-10
    93/100 stars

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    other:

    Article Title: Generation of CRISPR-Cas9 edited human induced pluripotent stem cell line carrying FLNC exon skipping variant.
    Article Snippet: Resource table Unique stem cell lines identifier ICANi002-A ICANi002-A-1 Alternative name(s) of stem cell lines ICAN-403.3 ICAN-FLNC42.1 Institution ICAN : Institut for Cardiometabolism And Nutrition Contact information of the reported cell line distributor Vincent Fontaine (v.fontaine@ican-instit ute.org) or Eric Villard (eric.villard@sor bonne-universite.fr) Type of cell lines iPSCs Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 30 Sex:M Ethnicity/breed/other genetic background information if known: Caucasian Cell Source Skin Fibroblasts Method of reprogramming Episomal vectors (Okita et al., 2011) Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) q-PCR negative for episomal vectors Cell culture system used Feeder free Type of Genetic Modification Induced mutation by CRISPR Cas9 in FLNC gene Associated disease OMIM FLNC-cardimyopathies: 617,047 Gene/locus FLNC, 7q32.1 (continued on next column) (continued ) Method of modification/site-specific nuclease used CRISPR/Cas9 induced non-homologus end-joining (NHEJ) Site-specific nuclease (SSN) delivery method Plasmid transfection, (pX459, Addgene #108293) All genetic material introduced into the cells - pX459V2.0-SpCas9-HF1 with specific gRNA coding sequence inserted (Addgene #108293) - pCX-cMyc (Addgene #19772) - pCXLE-hOCT3/4 (Addgene #27076) - pCXLE-hSK (Addgene #27078) Analysis of the nuclease-targeted allele status PCR and Sanger sequencing, and whole exome sequencing Method of the off-target nuclease activity surveillance Targeted PCR and sequencing and whole exome sequencing Name of transgene N/A Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific) Episomal Puromycin resistance in pX459V2.0-SpCas9-HF1 Inducible/constitutive system details N/A Date archived/stock date August 2021 Cell line repository/bank https://hpscreg.eu/cell-line/ICANi002-A https://hpscreg.eu/cell-line/ICANi002-A -1 Ethical/GMO work approvals Human fibroblast purchased from Promocell.

    Plasmid Preparation:

    Article Title: Quantitative analysis of CRISPR/Cas9-mediated provirus deletion in blue egg layer chicken PGCs by digital PCR.
    Article Snippet: .. The wildtype pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid was a gift from Feng Zhang (Addgene plasmid # 62988; http:// n2t. net/ addge ne: 62988; RRID: Addgene_62988)22 and the PX459V2.0-SpCas9-HF1 plasmid was a gift from Yuichiro Miyaoka (Addgene plasmid # 108293; http:// n2t. net/ addge ne: 108293; RRID: Addgene_108293)33. ..



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