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Spatial Transcriptomics Inc human hippocampus dataset
a Bright-field image and manually annotated segmentation of <t>hippocampus</t> layers and white matter (WM) in the human hippocampus. b Spatial clustering of hippocampal regions using MultiGATE, SpatialGlue, and Seurat WNN. Clustering performance is assessed using the Adjusted Rand Index (ARI), with higher values indicating greater clustering accuracy. c Box plots representing attention scores for peak–gene pairs across different genomic distances, grouped based on whether they are supported by expression quantitative trait loci (eQTL) evidence. The box plots indicate the medians (centerlines), means (triangles), first and third quartiles (bounds of boxes), and 1.5 × interquartile range (whiskers). Sample sizes per bin (False/True): 0–25 kb (621/222), 25–50 kb (479/88), 50–75 kb (461/78), 75–100 kb (469/44), 100–125 kb (446/30), 125–150 kb (405/29). d Receiver operating characteristic (ROC) curves comparing the performance of MultiGATE and other methods in predicting eQTL-associated regulatory interactions. e Visualization of MultiGATE-predicted cis-regulatory interactions for the target genes CA12 and PRKD3 along with eQTL evidence. Source data are provided as a Source Data file.
Human Hippocampus Dataset, supplied by Spatial Transcriptomics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spatial+transcriptomics+st+data/breast+data+her2+human+positive+spatial+transcriptomics+tumor/pmc12552752-274-3-12
Average 86 stars, based on 1 article reviews
human hippocampus dataset - by Bioz Stars, 2026-09
86/100 stars

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1) Product Images from "MultiGATE: integrative analysis and regulatory inference in spatial multi-omics data via graph representation learning"

Article Title: MultiGATE: integrative analysis and regulatory inference in spatial multi-omics data via graph representation learning

Journal: Nature Communications

doi: 10.1038/s41467-025-63418-x

a Bright-field image and manually annotated segmentation of hippocampus layers and white matter (WM) in the human hippocampus. b Spatial clustering of hippocampal regions using MultiGATE, SpatialGlue, and Seurat WNN. Clustering performance is assessed using the Adjusted Rand Index (ARI), with higher values indicating greater clustering accuracy. c Box plots representing attention scores for peak–gene pairs across different genomic distances, grouped based on whether they are supported by expression quantitative trait loci (eQTL) evidence. The box plots indicate the medians (centerlines), means (triangles), first and third quartiles (bounds of boxes), and 1.5 × interquartile range (whiskers). Sample sizes per bin (False/True): 0–25 kb (621/222), 25–50 kb (479/88), 50–75 kb (461/78), 75–100 kb (469/44), 100–125 kb (446/30), 125–150 kb (405/29). d Receiver operating characteristic (ROC) curves comparing the performance of MultiGATE and other methods in predicting eQTL-associated regulatory interactions. e Visualization of MultiGATE-predicted cis-regulatory interactions for the target genes CA12 and PRKD3 along with eQTL evidence. Source data are provided as a Source Data file.
Figure Legend Snippet: a Bright-field image and manually annotated segmentation of hippocampus layers and white matter (WM) in the human hippocampus. b Spatial clustering of hippocampal regions using MultiGATE, SpatialGlue, and Seurat WNN. Clustering performance is assessed using the Adjusted Rand Index (ARI), with higher values indicating greater clustering accuracy. c Box plots representing attention scores for peak–gene pairs across different genomic distances, grouped based on whether they are supported by expression quantitative trait loci (eQTL) evidence. The box plots indicate the medians (centerlines), means (triangles), first and third quartiles (bounds of boxes), and 1.5 × interquartile range (whiskers). Sample sizes per bin (False/True): 0–25 kb (621/222), 25–50 kb (479/88), 50–75 kb (461/78), 75–100 kb (469/44), 100–125 kb (446/30), 125–150 kb (405/29). d Receiver operating characteristic (ROC) curves comparing the performance of MultiGATE and other methods in predicting eQTL-associated regulatory interactions. e Visualization of MultiGATE-predicted cis-regulatory interactions for the target genes CA12 and PRKD3 along with eQTL evidence. Source data are provided as a Source Data file.

Techniques Used: Expressing

Related Articles

other:

Article Title: MultiGATE: integrative analysis and regulatory inference in spatial multi-omics data via graph representation learning
Article Snippet: For the adult human hippocampus dataset (spatial ATAC–RNA–seq), the mouse brain dataset (spatial transcriptomics + metabolomics), the breast cancer-patterned spatial ATAC + RNA dataset (Supplementary Fig. ), which have ground truth spatial domain annotations, we computed the following metrics to assess clustering agreement: Rand Index (RI), ARI, Adjusted Mutual Information, Normalized Mutual Information, Homogeneity, Completeness, V-measure, Fowlkes-Mallows Index.

Article Title: Bridging cell morphological behaviors and molecular dynamics in multi-modal spatial omics with MorphLink
Article Snippet: Publicly available data were acquired from the following websites or accession numbers: (1) human bladder tumor 10x Visium data (GEO repository: GSE246011 ); (2) zebrafish melanoma 10x Visium data (GEO repository: GSE159709 ); (3) human tonsil spatial CITE-seq data ( https://www.10xgenomics.com/datasets/gene-protein-expression-library-of-human-tonsil-cytassist-ffpe-2-standard ); (4) human HER2-positive breast tumor spatial transcriptomics data ( https://github.com/almaan/her2st ); (5) human breast tumor 10x Visium data ( https://www.10xgenomics.com/datasets/human-breast-cancer-visium-fresh-frozen-whole-transcriptome-1-standard ); (6) mouse brain 10x Visium data ( https://www.10xgenomics.com/datasets/fresh-frozen-visium-on-cytassist-mouse-brain-probe-based-whole-transcriptome-profiling-2-standard ); (7) mouse embryo 10x Visium data ( https://www.10xgenomics.com/datasets/visium-cytassist-mouse-embryo-11-mm-capture-area-ffpe-2-standard ); (8) human breast tumor H&E images from TCGA data ( https://portal.gdc.cancer.gov/ sample ID: DX1.01FB49CC, DX1.392580F3, DX1.0E26C46D).

Functional Assay:

Article Title: A spatially informed matrix normal model for gene co-expression analysis in spatial transcriptomics studies.
Article Snippet: .. The reduced overlap may indicate weakening of their functional connection in aging process. ouse and human MERFISH brain cortex data e conducted a cross-species gene co-expression analysis on uman and mouse brain cortex data [ 36 ] generated by MERISH, an imaging-based spatial transcriptomics technology 78 ]. ..

Generated:

Article Title: A spatially informed matrix normal model for gene co-expression analysis in spatial transcriptomics studies.
Article Snippet: .. The reduced overlap may indicate weakening of their functional connection in aging process. ouse and human MERFISH brain cortex data e conducted a cross-species gene co-expression analysis on uman and mouse brain cortex data [ 36 ] generated by MERISH, an imaging-based spatial transcriptomics technology 78 ]. ..

Imaging:

Article Title: A spatially informed matrix normal model for gene co-expression analysis in spatial transcriptomics studies.
Article Snippet: .. The reduced overlap may indicate weakening of their functional connection in aging process. ouse and human MERFISH brain cortex data e conducted a cross-species gene co-expression analysis on uman and mouse brain cortex data [ 36 ] generated by MERISH, an imaging-based spatial transcriptomics technology 78 ]. ..

Spatial Transcriptomics:

Article Title: A spatially informed matrix normal model for gene co-expression analysis in spatial transcriptomics studies.
Article Snippet: .. The reduced overlap may indicate weakening of their functional connection in aging process. ouse and human MERFISH brain cortex data e conducted a cross-species gene co-expression analysis on uman and mouse brain cortex data [ 36 ] generated by MERISH, an imaging-based spatial transcriptomics technology 78 ]. ..

Article Title: Transfer learning of multicellular organization via single-cell and spatial transcriptomics
Article Snippet: .. The original public data used in this paper can be accessed through the following links: (1) FISH data from the Berkeley Drosophila Transcription Network Project (BDTNP): https://shiny.mdc-berlin.de/DVEX/ ; (2) 10X Visium data of the human dorsolateral prefrontal cortex (DLPFC): http://spatial.libd.org/spatialLIBD/ ; (3) Adult mouse cortical cell datasets (GEO accession GSE71585): https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71585 ; (4) Mouse embryo data: https://content.cruk.cam.ac.uk/jmlab/SpatialMouseAtlas2020/ ; (5) Seurat objects ST data (10X Genomics Visium) of mouse brain: https://satijalab.org/seurat/articles/spatial_vignette.html ; (6) 10X Visium data of mouse brain: https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-11114 ; (7) MERFISH data of mouse brain: https://portal.brain-map.org/atlases-and-data/bkp/abc-atlas ; (8) MERFISH data of human MTG: https://doi.org/10.5061/dryad.x3ffbg7mw ; (9) SMART-seq data of human MTG: https://portal.brain-map.org/atlases-and-data/rnaseq/human-mtg-smart-seq ; (10) Single-cell RNA-seq and Spatial Transcriptomics data of developing human heart: https://data.mendeley.com/datasets/mbvhhf8m62/2 ; (11) Human artery data: https://doi.org/10.5281/zenodo.15132967 . ..

Article Title: Single-cell and spatial detection of senescent cells using DeepScence
Article Snippet: Spatial transcriptomics data from mouse muscle under notexin-induced injury , McKellar et al. , GEO: GSE161318. .. Spatial transcriptomics data from human brain with Alzheimer’s disease , Gong et al. , GEO: GSE269906. ..

Article Title: scGALA advances graph link prediction-based cell alignment for comprehensive data integration and harmonization
Article Snippet: .. Furthermore, we applied scGALA to a Xenium human breast cancer dataset (541 genes, accessible through SubcellularSpatialData R/Bioconductor package with dataset ID: EH8567) to demonstrate practical applicability on real spatial transcriptomics data with limited gene panels. ..

Article Title: Spatial transcriptome and single-cell sequencing reveal the role of nucleotide metabolism in breast cancer progression and tumor microenvironment
Article Snippet: .. Breast cancer spatial transcriptomics (ST) data were acquired from the GEO database ( https://www.ncbi.nlm.nih.gov/geo/ ) and 10x Genomics official website ( https://www.10xgenomics.com/ ). ..

Fluorescence In Situ Hybridization:

Article Title: Transfer learning of multicellular organization via single-cell and spatial transcriptomics
Article Snippet: .. The original public data used in this paper can be accessed through the following links: (1) FISH data from the Berkeley Drosophila Transcription Network Project (BDTNP): https://shiny.mdc-berlin.de/DVEX/ ; (2) 10X Visium data of the human dorsolateral prefrontal cortex (DLPFC): http://spatial.libd.org/spatialLIBD/ ; (3) Adult mouse cortical cell datasets (GEO accession GSE71585): https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71585 ; (4) Mouse embryo data: https://content.cruk.cam.ac.uk/jmlab/SpatialMouseAtlas2020/ ; (5) Seurat objects ST data (10X Genomics Visium) of mouse brain: https://satijalab.org/seurat/articles/spatial_vignette.html ; (6) 10X Visium data of mouse brain: https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-11114 ; (7) MERFISH data of mouse brain: https://portal.brain-map.org/atlases-and-data/bkp/abc-atlas ; (8) MERFISH data of human MTG: https://doi.org/10.5061/dryad.x3ffbg7mw ; (9) SMART-seq data of human MTG: https://portal.brain-map.org/atlases-and-data/rnaseq/human-mtg-smart-seq ; (10) Single-cell RNA-seq and Spatial Transcriptomics data of developing human heart: https://data.mendeley.com/datasets/mbvhhf8m62/2 ; (11) Human artery data: https://doi.org/10.5281/zenodo.15132967 . ..

Single Cell:

Article Title: Transfer learning of multicellular organization via single-cell and spatial transcriptomics
Article Snippet: .. The original public data used in this paper can be accessed through the following links: (1) FISH data from the Berkeley Drosophila Transcription Network Project (BDTNP): https://shiny.mdc-berlin.de/DVEX/ ; (2) 10X Visium data of the human dorsolateral prefrontal cortex (DLPFC): http://spatial.libd.org/spatialLIBD/ ; (3) Adult mouse cortical cell datasets (GEO accession GSE71585): https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71585 ; (4) Mouse embryo data: https://content.cruk.cam.ac.uk/jmlab/SpatialMouseAtlas2020/ ; (5) Seurat objects ST data (10X Genomics Visium) of mouse brain: https://satijalab.org/seurat/articles/spatial_vignette.html ; (6) 10X Visium data of mouse brain: https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-11114 ; (7) MERFISH data of mouse brain: https://portal.brain-map.org/atlases-and-data/bkp/abc-atlas ; (8) MERFISH data of human MTG: https://doi.org/10.5061/dryad.x3ffbg7mw ; (9) SMART-seq data of human MTG: https://portal.brain-map.org/atlases-and-data/rnaseq/human-mtg-smart-seq ; (10) Single-cell RNA-seq and Spatial Transcriptomics data of developing human heart: https://data.mendeley.com/datasets/mbvhhf8m62/2 ; (11) Human artery data: https://doi.org/10.5281/zenodo.15132967 . ..

RNA Sequencing:

Article Title: Transfer learning of multicellular organization via single-cell and spatial transcriptomics
Article Snippet: .. The original public data used in this paper can be accessed through the following links: (1) FISH data from the Berkeley Drosophila Transcription Network Project (BDTNP): https://shiny.mdc-berlin.de/DVEX/ ; (2) 10X Visium data of the human dorsolateral prefrontal cortex (DLPFC): http://spatial.libd.org/spatialLIBD/ ; (3) Adult mouse cortical cell datasets (GEO accession GSE71585): https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71585 ; (4) Mouse embryo data: https://content.cruk.cam.ac.uk/jmlab/SpatialMouseAtlas2020/ ; (5) Seurat objects ST data (10X Genomics Visium) of mouse brain: https://satijalab.org/seurat/articles/spatial_vignette.html ; (6) 10X Visium data of mouse brain: https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-11114 ; (7) MERFISH data of mouse brain: https://portal.brain-map.org/atlases-and-data/bkp/abc-atlas ; (8) MERFISH data of human MTG: https://doi.org/10.5061/dryad.x3ffbg7mw ; (9) SMART-seq data of human MTG: https://portal.brain-map.org/atlases-and-data/rnaseq/human-mtg-smart-seq ; (10) Single-cell RNA-seq and Spatial Transcriptomics data of developing human heart: https://data.mendeley.com/datasets/mbvhhf8m62/2 ; (11) Human artery data: https://doi.org/10.5281/zenodo.15132967 . ..

Sample Prep:

Article Title: Integrating Spatial Omics and Deep Learning: Toward Predictive Models of Cardiomyocyte Differentiation Efficiency
Article Snippet: Pathological/Regenerative , Spatial multi-omic map of human MI (Myocardial Infarction) , snRNA-seq + snATAC-seq + Spatial Transcriptomics , Human infarcted heart , Yes (Post-MI timepoints) , Post-MI inflammatory environments induce batch-specific effects; regression-based correction (e.g., Harmony) improves comparability. Challenges include disease-induced heterogeneity complicating alignment, integrating chromatin/epigenetic with spatial data, temporal variability across MI stages, and sparsity in infarct zones. These issues impact remodelling maps, potentially biassing therapeutic target identification. , [ ] . .. , Human heart spatial transcriptomics (Disease) , Spatial Transcriptomics , Human heart (disease) , No (Disease states) , Differences in sample preparation (frozen vs. FFPE) require careful normalisation (e.g., using sctransform or DESeq2). Challenges involve tissue quality variations in diseased samples, artefact removal from pathology-induced noise, ensuring comparability across disease states, and handling low-resolution spots in heterogeneous lesions. These limitations affect disease progression modelling, risking inaccurate spatial gene expression profiles. , [ , ] . .. , Zebrafish heart regeneration atlas , scRNA-seq + Spatial Transcriptomics (Stereo-seq) , Zebrafish heart , Yes (8 timepoints of zebrafish heart regeneration stages) , Cross-species integration requires ortholog mapping + dimensionality reduction alignment. Challenges include species-specific gene expression differences, handling high-resolution Stereo-seq data volumes, temporal alignment across regeneration stages, and batch effects from injury timepoints. These issues influence comparative regenerative studies, potentially leading to translational gaps with human models , [ ] .

Formalin-fixed Paraffin-Embedded:

Article Title: Integrating Spatial Omics and Deep Learning: Toward Predictive Models of Cardiomyocyte Differentiation Efficiency
Article Snippet: Pathological/Regenerative , Spatial multi-omic map of human MI (Myocardial Infarction) , snRNA-seq + snATAC-seq + Spatial Transcriptomics , Human infarcted heart , Yes (Post-MI timepoints) , Post-MI inflammatory environments induce batch-specific effects; regression-based correction (e.g., Harmony) improves comparability. Challenges include disease-induced heterogeneity complicating alignment, integrating chromatin/epigenetic with spatial data, temporal variability across MI stages, and sparsity in infarct zones. These issues impact remodelling maps, potentially biassing therapeutic target identification. , [ ] . .. , Human heart spatial transcriptomics (Disease) , Spatial Transcriptomics , Human heart (disease) , No (Disease states) , Differences in sample preparation (frozen vs. FFPE) require careful normalisation (e.g., using sctransform or DESeq2). Challenges involve tissue quality variations in diseased samples, artefact removal from pathology-induced noise, ensuring comparability across disease states, and handling low-resolution spots in heterogeneous lesions. These limitations affect disease progression modelling, risking inaccurate spatial gene expression profiles. , [ , ] . .. , Zebrafish heart regeneration atlas , scRNA-seq + Spatial Transcriptomics (Stereo-seq) , Zebrafish heart , Yes (8 timepoints of zebrafish heart regeneration stages) , Cross-species integration requires ortholog mapping + dimensionality reduction alignment. Challenges include species-specific gene expression differences, handling high-resolution Stereo-seq data volumes, temporal alignment across regeneration stages, and batch effects from injury timepoints. These issues influence comparative regenerative studies, potentially leading to translational gaps with human models , [ ] .

Biomarker Discovery:

Article Title: Integrating Spatial Omics and Deep Learning: Toward Predictive Models of Cardiomyocyte Differentiation Efficiency
Article Snippet: Pathological/Regenerative , Spatial multi-omic map of human MI (Myocardial Infarction) , snRNA-seq + snATAC-seq + Spatial Transcriptomics , Human infarcted heart , Yes (Post-MI timepoints) , Post-MI inflammatory environments induce batch-specific effects; regression-based correction (e.g., Harmony) improves comparability. Challenges include disease-induced heterogeneity complicating alignment, integrating chromatin/epigenetic with spatial data, temporal variability across MI stages, and sparsity in infarct zones. These issues impact remodelling maps, potentially biassing therapeutic target identification. , [ ] . .. , Human heart spatial transcriptomics (Disease) , Spatial Transcriptomics , Human heart (disease) , No (Disease states) , Differences in sample preparation (frozen vs. FFPE) require careful normalisation (e.g., using sctransform or DESeq2). Challenges involve tissue quality variations in diseased samples, artefact removal from pathology-induced noise, ensuring comparability across disease states, and handling low-resolution spots in heterogeneous lesions. These limitations affect disease progression modelling, risking inaccurate spatial gene expression profiles. , [ , ] . .. , Zebrafish heart regeneration atlas , scRNA-seq + Spatial Transcriptomics (Stereo-seq) , Zebrafish heart , Yes (8 timepoints of zebrafish heart regeneration stages) , Cross-species integration requires ortholog mapping + dimensionality reduction alignment. Challenges include species-specific gene expression differences, handling high-resolution Stereo-seq data volumes, temporal alignment across regeneration stages, and batch effects from injury timepoints. These issues influence comparative regenerative studies, potentially leading to translational gaps with human models , [ ] .

Gene Expression:

Article Title: Integrating Spatial Omics and Deep Learning: Toward Predictive Models of Cardiomyocyte Differentiation Efficiency
Article Snippet: Pathological/Regenerative , Spatial multi-omic map of human MI (Myocardial Infarction) , snRNA-seq + snATAC-seq + Spatial Transcriptomics , Human infarcted heart , Yes (Post-MI timepoints) , Post-MI inflammatory environments induce batch-specific effects; regression-based correction (e.g., Harmony) improves comparability. Challenges include disease-induced heterogeneity complicating alignment, integrating chromatin/epigenetic with spatial data, temporal variability across MI stages, and sparsity in infarct zones. These issues impact remodelling maps, potentially biassing therapeutic target identification. , [ ] . .. , Human heart spatial transcriptomics (Disease) , Spatial Transcriptomics , Human heart (disease) , No (Disease states) , Differences in sample preparation (frozen vs. FFPE) require careful normalisation (e.g., using sctransform or DESeq2). Challenges involve tissue quality variations in diseased samples, artefact removal from pathology-induced noise, ensuring comparability across disease states, and handling low-resolution spots in heterogeneous lesions. These limitations affect disease progression modelling, risking inaccurate spatial gene expression profiles. , [ , ] . .. , Zebrafish heart regeneration atlas , scRNA-seq + Spatial Transcriptomics (Stereo-seq) , Zebrafish heart , Yes (8 timepoints of zebrafish heart regeneration stages) , Cross-species integration requires ortholog mapping + dimensionality reduction alignment. Challenges include species-specific gene expression differences, handling high-resolution Stereo-seq data volumes, temporal alignment across regeneration stages, and batch effects from injury timepoints. These issues influence comparative regenerative studies, potentially leading to translational gaps with human models , [ ] .



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