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s ko 03334 morrbid  (Cyagen Biosciences)


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    Cyagen Biosciences s ko 03334 morrbid
    S Ko 03334 Morrbid, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spatial+transcriptomics+sequencing+methods+based+on+barcoded+array+chips/Ncf2/pm40272982-676-93-96
    Average 93 stars, based on 1 article reviews
    s ko 03334 morrbid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Gene Expression:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Biomarker Discovery:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Lysis:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Ligation:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    DNA Sequencing:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Double Staining:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Nitric Oxide Assay:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    RNA Sequencing:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Spatial Transcriptomics:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Software:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.

    Sequencing:

    Article Title: Single-cell and spatial transcriptomics reveal the pathogenesis of chronic granulomatous disease in a natural model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER EasySepTM Mouse Hematopoietic Progenitor Cell Isolation Kit STEMCELL Cat# 19856A Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit Elabscience Cat# E-EL-M0771 Mouse IL-1β ELISA Kit MULTI SCIENCES Cat# EK201B/3-48 Proinflammatory Panel 1 (mouse) kits MSD Cat# K15048D Sodium Dodecyl Sulfate (SDS) GenStar Cat# ZE153-101S Protein Free Rapid Blocking Buffer(5×) EpiZyme Cat# PS108 StarSignal Chemiluminescent Assay Kit Genstar Cat# E171-01 True-NuclearTM Transcription Factor Buffer Set BioLegend Cat# 424401 Reactive Oxygen Species Assay Kit Beyotime Cat# S0033S Tissue-Tek® O.C.T. .. Compound SAKURA Cat# 4583 SeekSpace sc-Spatial Labeling Kit V 1.0 SEEKGENE NA BMKMANU S1000 Gene Expression Kit Biomarker NA QIAzol Lysis Reagent QIAGEN Cat# 79306 Illumina Stranded mRNA Prep, Ligation kit Illumina Cat# 20040534 FastDNATM SPIN Kit for Soil MP Biomedicals Cat# 116560300 NEXTFLEX Rapid DNA-Seq Kit Bioo Scientific Cat# 5144-08 Annexin V-PE/7-AAD double staining apoptosis detection kit KeyGEN Cat# KGA1018 Cell and Tissue Lysis Buffer for Nitric Oxide Assay Beyotime Cat# S3090 Nitric oxide test kit Beyotime Cat# S0021S Experimental models: Organisms C57BL/6J Cyagen Biosciences Cat# C001089 Ncf2+/- C57BL/6J Cyagen Biosciences Cat# S-KO-03334 Morrbid+/- C57BL/6J Cyagen Biosciences Contract No. KOCMAIS211018ZQ7 Il1R1+/- C57BL/6J Cyagen Biosciences Cat# S-KO-02635 Deposited data Bulk RNA-seq This paper GSE270426 scRNA-seq This paper GSE271387 Spatial transcriptomics (SeekSpace) This paper GSE270427 Spatial transcriptomics (BMKMANU S1000 ) This paper GSE271222 Software and algorithms R R Core Team http://www.r-project.org/ RStudio RStudio https://rstudio.com/ ImageJ National Institutes of Health https://imagej.net/ij/ GraphPad Prism v.8.3.0 GraphPad https://www.graphpad.com/ Monocle3 NA https://cole-trapnell-lab.github.io/monocle3/ Seurat v4.2.0 Hao et al.67 https://satijalab.org/seurat/ FlowJo v10.0.7 BD Biosciences https://www.flowjo.com/solutions/flowjo pySCENIC v0.11.2 Van de Sande et al.68 https://github.com/aertslab/pySCENIC bcl2fastq2 v2.2.0 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html SeekSpace Tools v1.0.0 SEEKGENE http://seekspace.seekgene.com/ zh/v1.0.0/index.html BSTMatrix 1.0 Biomarker http://www.biomarker.com.cN/ Archives/28318 (Continued on next page) 18 Cell Reports 44, 115612, May 27, 2025 .. The C57BL/6 WT, Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were purchased from Cyagen Biosciences Inc. Morrbid+/-, Il1R1+/-, and Ncf2+/- mice were bred to generate homozygous individuals through self-crossing.



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