goat anti nephrin antibody (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology goat anti nephrin antibody

Detection of glucocorticoid-induced transcript <t>1</t> <t>(GLCCI1)</t> and podocyte-specific marker gene expression by reverse transcription-PCR (RT-PCR). ( a ) Expression of the genes encoding GLCCI1, <t>nephrin,</t> podocin, synaptopodin and podocalyxin showed similar patterns in the podocytes. The expression levels differed significantly between the high glucose-induced and wortmannin-treated diabetic groups of podocytes. ( b ) The expression of the genes encoding GLCCI1, nephrin, podocin, synaptopodin and podocalyxin showed similar patterns in the kidneys of diabetic rats. In addition, wortmannin ameliorated the expression of all podocyte-specific proteins including GLCCI1. ( c , d ) The relative band intensity of proteins in podocytes and the kidneys of diabetic rats was observed by RT-PCR. The band intensity was measured using the Multi Gauge V3.0 software (Fuji Film). *** P <0.001, 5 m M (control) and wortmannin-treated diabetic groups versus the high glucose–induced group by analysis of variance (ANOVA; means±s.e.m., n =3).

Goat Anti Nephrin Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spatial+transcriptomics+cell-type+deconvolution/pmc04910149-56-25-29?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 293 article reviews

goat anti nephrin antibody - by Bioz Stars, 2026-07

96/100 stars

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1) Product Images from "GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes"

Article Title: GLCCI1 is a novel component associated with the PI3K signaling pathway in podocyte foot processes

Journal: Experimental & Molecular Medicine

doi: 10.1038/emm.2016.28

Detection of glucocorticoid-induced transcript 1 (GLCCI1) and podocyte-specific marker gene expression by reverse transcription-PCR (RT-PCR). ( a ) Expression of the genes encoding GLCCI1, nephrin, podocin, synaptopodin and podocalyxin showed similar patterns in the podocytes. The expression levels differed significantly between the high glucose-induced and wortmannin-treated diabetic groups of podocytes. ( b ) The expression of the genes encoding GLCCI1, nephrin, podocin, synaptopodin and podocalyxin showed similar patterns in the kidneys of diabetic rats. In addition, wortmannin ameliorated the expression of all podocyte-specific proteins including GLCCI1. ( c , d ) The relative band intensity of proteins in podocytes and the kidneys of diabetic rats was observed by RT-PCR. The band intensity was measured using the Multi Gauge V3.0 software (Fuji Film). *** P <0.001, 5 m M (control) and wortmannin-treated diabetic groups versus the high glucose–induced group by analysis of variance (ANOVA; means±s.e.m., n =3).

Figure Legend Snippet: Detection of glucocorticoid-induced transcript 1 (GLCCI1) and podocyte-specific marker gene expression by reverse transcription-PCR (RT-PCR). ( a ) Expression of the genes encoding GLCCI1, nephrin, podocin, synaptopodin and podocalyxin showed similar patterns in the podocytes. The expression levels differed significantly between the high glucose-induced and wortmannin-treated diabetic groups of podocytes. ( b ) The expression of the genes encoding GLCCI1, nephrin, podocin, synaptopodin and podocalyxin showed similar patterns in the kidneys of diabetic rats. In addition, wortmannin ameliorated the expression of all podocyte-specific proteins including GLCCI1. ( c , d ) The relative band intensity of proteins in podocytes and the kidneys of diabetic rats was observed by RT-PCR. The band intensity was measured using the Multi Gauge V3.0 software (Fuji Film). *** P <0.001, 5 m M (control) and wortmannin-treated diabetic groups versus the high glucose–induced group by analysis of variance (ANOVA; means±s.e.m., n =3).

Techniques Used: Marker, Gene Expression, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Expressing, Software, Control

Analysis of glucocorticoid-induced transcript 1 (GLCCI1) protein expression and involvement in the phosphoinositide 3-kinase (PI3K) pathway by western blotting. ( a ) GLCCI1, nephrin and podocin were expressed only in the 5 m M and the wortmannin-treated diabetic groups. Serum/glucocorticoid-regulated kinase 1 (SGK1) was expressed only in the high glucose-induced group. Forkhead box O3 (FOXO3A) was regulated by wortmannin. ( b ) GLCCI1 was expressed only in the control and wortmannin-treated diabetic groups. GLCCI1 was significantly decreased in the diabetic group. ( c , d ) The relative band intensities of proteins in the podocytes and the kidneys of diabetic rats were observed by western blot analysis. The band intensities were measured using the Multi Gauge V3.0 software (Fuji Film). *** P <0.001, 5 m M (control) and wortmannin-treated diabetic groups versus the 25 m M (diabetes) group by analysis of variance (ANOVA; means±s.e.m., n =3).

Figure Legend Snippet: Analysis of glucocorticoid-induced transcript 1 (GLCCI1) protein expression and involvement in the phosphoinositide 3-kinase (PI3K) pathway by western blotting. ( a ) GLCCI1, nephrin and podocin were expressed only in the 5 m M and the wortmannin-treated diabetic groups. Serum/glucocorticoid-regulated kinase 1 (SGK1) was expressed only in the high glucose-induced group. Forkhead box O3 (FOXO3A) was regulated by wortmannin. ( b ) GLCCI1 was expressed only in the control and wortmannin-treated diabetic groups. GLCCI1 was significantly decreased in the diabetic group. ( c , d ) The relative band intensities of proteins in the podocytes and the kidneys of diabetic rats were observed by western blot analysis. The band intensities were measured using the Multi Gauge V3.0 software (Fuji Film). *** P <0.001, 5 m M (control) and wortmannin-treated diabetic groups versus the 25 m M (diabetes) group by analysis of variance (ANOVA; means±s.e.m., n =3).

Techniques Used: Expressing, Western Blot, Control, Software

The localization of glucocorticoid-induced transcript 1 (GLCCI1), podocyte-specific markers and proteins involved in the phosphoinositide 3-kinase (PI3K) signaling pathway in podocytes. ( a ) In podocytes, GLCCI1 (red, Alexa 568 conjugated) was localized in the 4',6-diamidino-2-phenylindole (DAPI)-stained nuclei. No GLCCI1 localization was observed in the 25 m M D -glucose-treated podocytes. However, GLCCI1 was regulated by wortmannin treatment. ( b – d ) The podocyte-specific proteins nephrin (green, Alexa 488 conjugated), podocin (red) and synaptopodin (green) showed a reactivity pattern similar to GLCCI1 in podocytes treated with wortmannin. ( e ) Serum/glucocorticoid-regulated kinase 1 (SGK1; red) was observed only in the 25 m M group. We confirmed that treatment with wortmannin decreased SGK1 expression. ( f ) Forkhead box O3 (FOXO3A; red) was observed in the 5 m M group. A lower FOXO3A signal was accompanied by high reactivity of SGK1 in the 25 m M group. Localization of FOXO3A was regulated by treatment with wortmannin. Original magnification: × 400. Scale bar, 50 μm.

Figure Legend Snippet: The localization of glucocorticoid-induced transcript 1 (GLCCI1), podocyte-specific markers and proteins involved in the phosphoinositide 3-kinase (PI3K) signaling pathway in podocytes. ( a ) In podocytes, GLCCI1 (red, Alexa 568 conjugated) was localized in the 4',6-diamidino-2-phenylindole (DAPI)-stained nuclei. No GLCCI1 localization was observed in the 25 m M D -glucose-treated podocytes. However, GLCCI1 was regulated by wortmannin treatment. ( b – d ) The podocyte-specific proteins nephrin (green, Alexa 488 conjugated), podocin (red) and synaptopodin (green) showed a reactivity pattern similar to GLCCI1 in podocytes treated with wortmannin. ( e ) Serum/glucocorticoid-regulated kinase 1 (SGK1; red) was observed only in the 25 m M group. We confirmed that treatment with wortmannin decreased SGK1 expression. ( f ) Forkhead box O3 (FOXO3A; red) was observed in the 5 m M group. A lower FOXO3A signal was accompanied by high reactivity of SGK1 in the 25 m M group. Localization of FOXO3A was regulated by treatment with wortmannin. Original magnification: × 400. Scale bar, 50 μm.

Techniques Used: Staining, Expressing

The localization of glucocorticoid-induced transcript 1 (GLCCI1), podocyte-specific markers and proteins associated with the phosphoinositide 3-kinase (PI3K) signaling pathway in the rat glomerulus. ( a ) Staining of rat kidney sections with an antibody against GLCCI1 (red) is shown. The diabetic group showed low reactivity. However, GLCCI1 was regulated by wortmannin treatment. ( b – d ) The podocyte-specific proteins nephrin (green), podocin (red) and synaptopodin (green) showed a reactivity pattern similar to GLCCI1 following wortmannin treatment. ( e ) Serum/glucocorticoid-regulated kinase 1 (SGK1; red) was observed only in the diabetic group. We confirmed that SGK1 expression was decreased by wortmannin. ( f ) Forkhead box O3 (FOXO3A; red) was observed in the control group. The lower reactivity of FOXO3A was accompanied by the overexpression of SGK1 in the diabetic group. The localization of FOXO3A was regulated by wortmannin treatment. Original magnification: × 400. Scale bar, 50 μm.

Figure Legend Snippet: The localization of glucocorticoid-induced transcript 1 (GLCCI1), podocyte-specific markers and proteins associated with the phosphoinositide 3-kinase (PI3K) signaling pathway in the rat glomerulus. ( a ) Staining of rat kidney sections with an antibody against GLCCI1 (red) is shown. The diabetic group showed low reactivity. However, GLCCI1 was regulated by wortmannin treatment. ( b – d ) The podocyte-specific proteins nephrin (green), podocin (red) and synaptopodin (green) showed a reactivity pattern similar to GLCCI1 following wortmannin treatment. ( e ) Serum/glucocorticoid-regulated kinase 1 (SGK1; red) was observed only in the diabetic group. We confirmed that SGK1 expression was decreased by wortmannin. ( f ) Forkhead box O3 (FOXO3A; red) was observed in the control group. The lower reactivity of FOXO3A was accompanied by the overexpression of SGK1 in the diabetic group. The localization of FOXO3A was regulated by wortmannin treatment. Original magnification: × 400. Scale bar, 50 μm.

Techniques Used: Staining, Expressing, Control, Over Expression

Colocalization of glucocorticoid-induced transcript 1 (GLCCI1) with the podocyte-specific proteins nephrin and synaptopodin. ( a ) Double labeling with the podocyte-specific marker nephrin (green) showed a complete overlap (merge, yellow) with the distribution of GLCCI1 (red) in the 5 m M and wortmannin-treated diabetic groups of cultured podocytes. ( b ) GLCCI1 (red) also colocalized with synaptopodin (green). We observed overlapping reactivity (yellow indicates red plus green) in the 5 m M and wortmannin-treated diabetic groups of cultured podocytes. ( c , d ) In rat glomeruli, we observed overlapping reactivity (yellow) via the colocalization of GLCCI1 (red) with nephrin and synaptopodin (green). Wortmannin ameliorated the lower reactivity in glomeruli from rats in the diabetic group. Original magnification: × 400. Scale bar, 50 μm.

Figure Legend Snippet: Colocalization of glucocorticoid-induced transcript 1 (GLCCI1) with the podocyte-specific proteins nephrin and synaptopodin. ( a ) Double labeling with the podocyte-specific marker nephrin (green) showed a complete overlap (merge, yellow) with the distribution of GLCCI1 (red) in the 5 m M and wortmannin-treated diabetic groups of cultured podocytes. ( b ) GLCCI1 (red) also colocalized with synaptopodin (green). We observed overlapping reactivity (yellow indicates red plus green) in the 5 m M and wortmannin-treated diabetic groups of cultured podocytes. ( c , d ) In rat glomeruli, we observed overlapping reactivity (yellow) via the colocalization of GLCCI1 (red) with nephrin and synaptopodin (green). Wortmannin ameliorated the lower reactivity in glomeruli from rats in the diabetic group. Original magnification: × 400. Scale bar, 50 μm.

Techniques Used: Labeling, Marker, Cell Culture

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goat anti nephrin antibody (Santa Cruz Biotechnology)

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