β2m (ATCC)

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ATCC β2m

Chlamydia inhibits both constitutive and IFN-γ–inducible MHC class I expression in infected cells. HeLa cells with or without chlamydial infection were stimulated with IFN-γ or unstimulated and collected for flow cytometry (A), Western blot (B and C), and RT-PCR (E) analysis. Chlamydia prevents both constitutive and IFN-γ–inducible HLA-A, -B, and -C heavy chain and <t>β2M</t> surface expression (A). Chlamydia suppresses the total cellular protein level of both constitutive and IFN-γ–inducible β2M (B) and HLA-A and -B heavy chains (C) in various human cell lines. Chlamydia inhibits the mRNA expression of both MHC class I heavy chains and β2M (E). For pulse–chase labeling experiment (D), HL cells with or without chlamydial infection were metabolically labeled with S 35 –methionine/cysteine for 30 min, and the pulsed cell samples were aliquoted and chased for various times as indicated. Mature or immature bands correspond to proteins resistant or sensitive to EndoH digestion as determined in a separate experiment (data not shown).

β2m, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spatial+transcriptomics+cell-type+deconvolution/pmc02213440-39-11-13?v=ATCC
Average 93 stars, based on 24 article reviews

β2m - by Bioz Stars, 2026-08

93/100 stars

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1) Product Images from "Degradation of Transcription Factor Rfx5 during the Inhibition of Both Constitutive and Interferon γ–Inducible Major Histocompatibility Complex Class I Expression in Chlamydia-Infected Cells"

Article Title: Degradation of Transcription Factor Rfx5 during the Inhibition of Both Constitutive and Interferon γ–Inducible Major Histocompatibility Complex Class I Expression in Chlamydia-Infected Cells

Journal: The Journal of Experimental Medicine

doi:

Figure Legend Snippet: Chlamydia inhibits both constitutive and IFN-γ–inducible MHC class I expression in infected cells. HeLa cells with or without chlamydial infection were stimulated with IFN-γ or unstimulated and collected for flow cytometry (A), Western blot (B and C), and RT-PCR (E) analysis. Chlamydia prevents both constitutive and IFN-γ–inducible HLA-A, -B, and -C heavy chain and β2M surface expression (A). Chlamydia suppresses the total cellular protein level of both constitutive and IFN-γ–inducible β2M (B) and HLA-A and -B heavy chains (C) in various human cell lines. Chlamydia inhibits the mRNA expression of both MHC class I heavy chains and β2M (E). For pulse–chase labeling experiment (D), HL cells with or without chlamydial infection were metabolically labeled with S 35 –methionine/cysteine for 30 min, and the pulsed cell samples were aliquoted and chased for various times as indicated. Mature or immature bands correspond to proteins resistant or sensitive to EndoH digestion as determined in a separate experiment (data not shown).

Techniques Used: Expressing, Infection, Flow Cytometry, Western Blot, Reverse Transcription Polymerase Chain Reaction, Pulse Chase, Labeling, Metabolic Labelling

$Inhibition of MHC class I expression correlates with RFX5 degradation. (A) HeLa cells with or without chlamydial infection and with or without IFN-γ stimulation were analyzed on Western blot for the levels of RFX5, HLA-A and -B heavy chains, β2M, β1 integrin (as a control membrane protein), and a chlamydial MOMP. Chlamydia-infected cell samples showed reduced levels of HLA heavy chain and β2M and complete loss of RFX5. (B) Cell-free assay analysis of HLA-A and -B heavy chain, β2M, and RFX5 degradation. Cytosolic S100 fractions from either normal HeLa (HeLa S100) or chlamydia-infected HeLa cells (chlamydia S100) were incubated with the corresponding target proteins (either a membrane protein extract as the source of HLA-A and -B heavy chains and β2M or a nuclear protein extract as the source of RFX5). The incubated mixtures were analyzed on Western blot for the levels of the residual target proteins with the corresponding antibodies. Chlamydia S100 selectively degrades RFX5 but not HLA-A and -B heavy chains and β2M.$
Figure Legend Snippet: Inhibition of MHC class I expression correlates with RFX5 degradation. (A) HeLa cells with or without chlamydial infection and with or without IFN-γ stimulation were analyzed on Western blot for the levels of RFX5, HLA-A and -B heavy chains, β2M, β1 integrin (as a control membrane protein), and a chlamydial MOMP. Chlamydia-infected cell samples showed reduced levels of HLA heavy chain and β2M and complete loss of RFX5. (B) Cell-free assay analysis of HLA-A and -B heavy chain, β2M, and RFX5 degradation. Cytosolic S100 fractions from either normal HeLa (HeLa S100) or chlamydia-infected HeLa cells (chlamydia S100) were incubated with the corresponding target proteins (either a membrane protein extract as the source of HLA-A and -B heavy chains and β2M or a nuclear protein extract as the source of RFX5). The incubated mixtures were analyzed on Western blot for the levels of the residual target proteins with the corresponding antibodies. Chlamydia S100 selectively degrades RFX5 but not HLA-A and -B heavy chains and β2M.

Techniques Used: Inhibition, Expressing, Infection, Western Blot, Control, Membrane, Cell-Free Assay, Incubation

Chlamydial but not host protein synthesis is required for both the degradation of RFX5 and suppression of HLA-A and -B heavy chains and β2M. (A) Correlation between infection dose and RFX5 degradation. 30 h after chlamydial infection at various MOI, HeLa cells were analyzed for the levels of chlamydial MOMP and host RFX5, USF-1, and USF-2 proteins on Western blot. The anti–USF-2 antibody used in this experiment can bind to both USF-1 and -2. These two USF isoforms can be separated on blots if gels are run far enough. Since we have previously shown that USF-1 is degraded in chlamydia-infected cells , USF-1 is used as a positive control. Since USF-2 protein level is usually not altered by chlamydial infection, it serves as a negative control. ns denotes nonspecific binding. (B) Time course relationship between chlamydial growth and RFX5 degradation. At various time points after infection, HeLa cell samples were analyzed on Western blot as described in A. (C) Inhibition of chlamydial but not host protein synthesis prevents RFX5 degradation and restores HLA-A and -B heavy chain and β2M expression. Rifampin (RF; final concentration = 0.1 μg/ml), chloramphenicol (CH; 60 μg/ml) and penicillin (PG; 100 μg/ml) were added at the beginning of chlamydial infection and maintained throughout the culture. Cycloheximide (CY; 10 μg/ml) was added to the culture 10 h before IFN-γ treatment and maintained during the IFN-γ stimulation. The treated HeLa cells were analyzed for protein levels of HLA-A and -B heavy chains, β2M, RFX5, USF-1, and chlamydial MOMP on Western blot. ns denotes nonspecific binding.

Figure Legend Snippet: Chlamydial but not host protein synthesis is required for both the degradation of RFX5 and suppression of HLA-A and -B heavy chains and β2M. (A) Correlation between infection dose and RFX5 degradation. 30 h after chlamydial infection at various MOI, HeLa cells were analyzed for the levels of chlamydial MOMP and host RFX5, USF-1, and USF-2 proteins on Western blot. The anti–USF-2 antibody used in this experiment can bind to both USF-1 and -2. These two USF isoforms can be separated on blots if gels are run far enough. Since we have previously shown that USF-1 is degraded in chlamydia-infected cells , USF-1 is used as a positive control. Since USF-2 protein level is usually not altered by chlamydial infection, it serves as a negative control. ns denotes nonspecific binding. (B) Time course relationship between chlamydial growth and RFX5 degradation. At various time points after infection, HeLa cell samples were analyzed on Western blot as described in A. (C) Inhibition of chlamydial but not host protein synthesis prevents RFX5 degradation and restores HLA-A and -B heavy chain and β2M expression. Rifampin (RF; final concentration = 0.1 μg/ml), chloramphenicol (CH; 60 μg/ml) and penicillin (PG; 100 μg/ml) were added at the beginning of chlamydial infection and maintained throughout the culture. Cycloheximide (CY; 10 μg/ml) was added to the culture 10 h before IFN-γ treatment and maintained during the IFN-γ stimulation. The treated HeLa cells were analyzed for protein levels of HLA-A and -B heavy chains, β2M, RFX5, USF-1, and chlamydial MOMP on Western blot. ns denotes nonspecific binding.

Techniques Used: Infection, Western Blot, Positive Control, Negative Control, Binding Assay, Inhibition, Expressing, Concentration Assay

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1) Product Images from "Degradation of Transcription Factor Rfx5 during the Inhibition of Both Constitutive and Interferon γ–Inducible Major Histocompatibility Complex Class I Expression in Chlamydia-Infected Cells"

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