Expressing:Article Title: Targeting SPAK suppresses progression and averts an immune exhaustive microenvironment in hepatocellular carcinoma.
Article Snippet: .. Combined targeting SPAK and PD-1 showed enhanced efficacy in HCC. (a) Representative tissue region maps and expression analysis of SPAK levels from a spatial transcriptome dataset of ICI responders and non-responders (Mendeley Data: skrx2fz79n). (b) Representative abdominal MRI images, representative IHC staining and quantitative IHC analysis of SPAK levels performed on human HCC tissue obtained before and after treatment of ICI responders (n = 7 patients) and non-responders (n = 12 patients) (scale bars, 100 μm). (c) A schematic representation of the treatment strategy in the DEN/CCl4-induced HCC model. (d-e) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden (n = 5 mice) from the indicated DEN/CCl4-induced HCC model. (f) A schematic representation of the treatment strategy in the AKT/NRasV12-induced HCC model. (g-h) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden (n = 5 mice) from the indicated AKT/NRasV12-induced HCC model. (i) A schematic representation of the treatment strategy in the Hepa1-6-induced HCC model. (j-k) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 200 μm), and tumor burden (n = 6 mice) from the indicated Hepa1-6-induced HCC model. (l-m) Total flux curve and bioluminescence images of mice from the indicated Hepa1-6-induced HCC model for the indicated days (n = 6 mice); a, b, e, h, k, m P values were calculated using an unpaired t test (two-tailed). ..
Article Title: Targeting SPAK suppresses progression and averts an immune exhaustive microenvironment in hepatocellular carcinoma
Article Snippet: .. Fig. 8 Combined targeting SPAK and PD-1 showed enhanced efficacy in HCC. a Representative tissue region maps and expression analysis of SPAK levels from a spatial transcriptome dataset of ICI responders and non-responders (Mendeley Data: skrx2fz79n). b Representative abdominal MRI images, representative IHC staining and quantitative IHC analysis of SPAK levels performed on human HCC tissue obtained before and after treatment of ICI responders ( n = 7 patients) and non-responders ( n = 12 patients) (scale bars, 100 μm). c A schematic representation of the treatment strategy in the DEN/CCl4-induced HCC model. d , e Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden ( n = 5 mice) from the indicated DEN/CCl4-induced HCC model. f A schematic representation of the treatment strategy in the AKT/NRasV12 -induced HCC model. g , h Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden ( n = 5 mice) from the indicated AKT/NRasV12-induced HCC model. i A schematic representation of the treatment strategy in the Hepa1-6-induced HCC model. j , k Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 200 μm), and tumor burden ( n = 6 mice) from the indicated Hepa1-6-induced HCC model. l , m Total flux curve and bioluminescence images of mice from the indicated Hepa1-6-induced HCC model for the indicated days ( n = 6 mice); a , b , e , h , k , m P values were calculated using an unpaired t test (two-tailed). ..
Magnetic Resonance Imaging:Article Title: Targeting SPAK suppresses progression and averts an immune exhaustive microenvironment in hepatocellular carcinoma.
Article Snippet: .. Combined targeting SPAK and PD-1 showed enhanced efficacy in HCC. (a) Representative tissue region maps and expression analysis of SPAK levels from a spatial transcriptome dataset of ICI responders and non-responders (Mendeley Data: skrx2fz79n). (b) Representative abdominal MRI images, representative IHC staining and quantitative IHC analysis of SPAK levels performed on human HCC tissue obtained before and after treatment of ICI responders (n = 7 patients) and non-responders (n = 12 patients) (scale bars, 100 μm). (c) A schematic representation of the treatment strategy in the DEN/CCl4-induced HCC model. (d-e) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden (n = 5 mice) from the indicated DEN/CCl4-induced HCC model. (f) A schematic representation of the treatment strategy in the AKT/NRasV12-induced HCC model. (g-h) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden (n = 5 mice) from the indicated AKT/NRasV12-induced HCC model. (i) A schematic representation of the treatment strategy in the Hepa1-6-induced HCC model. (j-k) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 200 μm), and tumor burden (n = 6 mice) from the indicated Hepa1-6-induced HCC model. (l-m) Total flux curve and bioluminescence images of mice from the indicated Hepa1-6-induced HCC model for the indicated days (n = 6 mice); a, b, e, h, k, m P values were calculated using an unpaired t test (two-tailed). ..
Article Title: Targeting SPAK suppresses progression and averts an immune exhaustive microenvironment in hepatocellular carcinoma
Article Snippet: .. Fig. 8 Combined targeting SPAK and PD-1 showed enhanced efficacy in HCC. a Representative tissue region maps and expression analysis of SPAK levels from a spatial transcriptome dataset of ICI responders and non-responders (Mendeley Data: skrx2fz79n). b Representative abdominal MRI images, representative IHC staining and quantitative IHC analysis of SPAK levels performed on human HCC tissue obtained before and after treatment of ICI responders ( n = 7 patients) and non-responders ( n = 12 patients) (scale bars, 100 μm). c A schematic representation of the treatment strategy in the DEN/CCl4-induced HCC model. d , e Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden ( n = 5 mice) from the indicated DEN/CCl4-induced HCC model. f A schematic representation of the treatment strategy in the AKT/NRasV12 -induced HCC model. g , h Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden ( n = 5 mice) from the indicated AKT/NRasV12-induced HCC model. i A schematic representation of the treatment strategy in the Hepa1-6-induced HCC model. j , k Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 200 μm), and tumor burden ( n = 6 mice) from the indicated Hepa1-6-induced HCC model. l , m Total flux curve and bioluminescence images of mice from the indicated Hepa1-6-induced HCC model for the indicated days ( n = 6 mice); a , b , e , h , k , m P values were calculated using an unpaired t test (two-tailed). ..
Immunohistochemistry:Article Title: Targeting SPAK suppresses progression and averts an immune exhaustive microenvironment in hepatocellular carcinoma.
Article Snippet: .. Combined targeting SPAK and PD-1 showed enhanced efficacy in HCC. (a) Representative tissue region maps and expression analysis of SPAK levels from a spatial transcriptome dataset of ICI responders and non-responders (Mendeley Data: skrx2fz79n). (b) Representative abdominal MRI images, representative IHC staining and quantitative IHC analysis of SPAK levels performed on human HCC tissue obtained before and after treatment of ICI responders (n = 7 patients) and non-responders (n = 12 patients) (scale bars, 100 μm). (c) A schematic representation of the treatment strategy in the DEN/CCl4-induced HCC model. (d-e) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden (n = 5 mice) from the indicated DEN/CCl4-induced HCC model. (f) A schematic representation of the treatment strategy in the AKT/NRasV12-induced HCC model. (g-h) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden (n = 5 mice) from the indicated AKT/NRasV12-induced HCC model. (i) A schematic representation of the treatment strategy in the Hepa1-6-induced HCC model. (j-k) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 200 μm), and tumor burden (n = 6 mice) from the indicated Hepa1-6-induced HCC model. (l-m) Total flux curve and bioluminescence images of mice from the indicated Hepa1-6-induced HCC model for the indicated days (n = 6 mice); a, b, e, h, k, m P values were calculated using an unpaired t test (two-tailed). ..
Article Title: Targeting SPAK suppresses progression and averts an immune exhaustive microenvironment in hepatocellular carcinoma
Article Snippet: .. Fig. 8 Combined targeting SPAK and PD-1 showed enhanced efficacy in HCC. a Representative tissue region maps and expression analysis of SPAK levels from a spatial transcriptome dataset of ICI responders and non-responders (Mendeley Data: skrx2fz79n). b Representative abdominal MRI images, representative IHC staining and quantitative IHC analysis of SPAK levels performed on human HCC tissue obtained before and after treatment of ICI responders ( n = 7 patients) and non-responders ( n = 12 patients) (scale bars, 100 μm). c A schematic representation of the treatment strategy in the DEN/CCl4-induced HCC model. d , e Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden ( n = 5 mice) from the indicated DEN/CCl4-induced HCC model. f A schematic representation of the treatment strategy in the AKT/NRasV12 -induced HCC model. g , h Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden ( n = 5 mice) from the indicated AKT/NRasV12-induced HCC model. i A schematic representation of the treatment strategy in the Hepa1-6-induced HCC model. j , k Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 200 μm), and tumor burden ( n = 6 mice) from the indicated Hepa1-6-induced HCC model. l , m Total flux curve and bioluminescence images of mice from the indicated Hepa1-6-induced HCC model for the indicated days ( n = 6 mice); a , b , e , h , k , m P values were calculated using an unpaired t test (two-tailed). ..
Staining:Article Title: Targeting SPAK suppresses progression and averts an immune exhaustive microenvironment in hepatocellular carcinoma.
Article Snippet: .. Combined targeting SPAK and PD-1 showed enhanced efficacy in HCC. (a) Representative tissue region maps and expression analysis of SPAK levels from a spatial transcriptome dataset of ICI responders and non-responders (Mendeley Data: skrx2fz79n). (b) Representative abdominal MRI images, representative IHC staining and quantitative IHC analysis of SPAK levels performed on human HCC tissue obtained before and after treatment of ICI responders (n = 7 patients) and non-responders (n = 12 patients) (scale bars, 100 μm). (c) A schematic representation of the treatment strategy in the DEN/CCl4-induced HCC model. (d-e) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden (n = 5 mice) from the indicated DEN/CCl4-induced HCC model. (f) A schematic representation of the treatment strategy in the AKT/NRasV12-induced HCC model. (g-h) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden (n = 5 mice) from the indicated AKT/NRasV12-induced HCC model. (i) A schematic representation of the treatment strategy in the Hepa1-6-induced HCC model. (j-k) Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 200 μm), and tumor burden (n = 6 mice) from the indicated Hepa1-6-induced HCC model. (l-m) Total flux curve and bioluminescence images of mice from the indicated Hepa1-6-induced HCC model for the indicated days (n = 6 mice); a, b, e, h, k, m P values were calculated using an unpaired t test (two-tailed). ..
Article Title: Targeting SPAK suppresses progression and averts an immune exhaustive microenvironment in hepatocellular carcinoma
Article Snippet: .. Fig. 8 Combined targeting SPAK and PD-1 showed enhanced efficacy in HCC. a Representative tissue region maps and expression analysis of SPAK levels from a spatial transcriptome dataset of ICI responders and non-responders (Mendeley Data: skrx2fz79n). b Representative abdominal MRI images, representative IHC staining and quantitative IHC analysis of SPAK levels performed on human HCC tissue obtained before and after treatment of ICI responders ( n = 7 patients) and non-responders ( n = 12 patients) (scale bars, 100 μm). c A schematic representation of the treatment strategy in the DEN/CCl4-induced HCC model. d , e Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden ( n = 5 mice) from the indicated DEN/CCl4-induced HCC model. f A schematic representation of the treatment strategy in the AKT/NRasV12 -induced HCC model. g , h Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 1000 μm), and tumor burden ( n = 5 mice) from the indicated AKT/NRasV12-induced HCC model. i A schematic representation of the treatment strategy in the Hepa1-6-induced HCC model. j , k Gross images of livers (scale bars, 5 mm), representative H&E staining (scale bars, 200 μm), and tumor burden ( n = 6 mice) from the indicated Hepa1-6-induced HCC model. l , m Total flux curve and bioluminescence images of mice from the indicated Hepa1-6-induced HCC model for the indicated days ( n = 6 mice); a , b , e , h , k , m P values were calculated using an unpaired t test (two-tailed). ..
Gene Expression:
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