riboprobe system sp6/t7 kit (Promega)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Promega riboprobe system sp6/t7 kit
Riboprobe System Sp6/T7 Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sp6+maxi+script+kit/rabbit+reticulocyte+lysate/pmc05291978-32-14-13
Average 90 stars, based on 1 article reviews

riboprobe system sp6/t7 kit - by Bioz Stars, 2026-11

90/100 stars

Images

Generated:

Article Title: Supporting Information for The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins
Article Snippet: .. Measurement of intracellular Ca2+ levels, [Ca2+]i Three double-stranded RNA (dsRNA) specifically targeting the transient receptor potential canonical (TRPC) Hl-16258 and Hl-21915 and the piezo-type mechanosensitive ion channel component 2 (PIEZ2) Hl-13522 were designed based on their coding sequences. dsRNAs were generated with a T7 RiboMAX Express RNAi System kit (Promega, Madison, WI, USA). .. In addition, a non-targeting dsRNA, which is specific to enhanced green fluorescent protein (EGFP), was used as a negative control. dsRNA quality was verified after annealing by gel electrophoresis. dsRNA sequences for Hl-21915, Hl-16258, Hl-13522 and EGFP are as shown in SI Appendix, Dataset S1 W. Concentrations of synthesized dsRNA were verified with a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). dsRNAs were then diluted to 600 ng/μL in 150 mM RNasefree saline solution (RFSS).

Article Title: Supporting Information for The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins
Article Snippet: .. RNA interference (RNAi) Double-stranded RNA (dsRNA) specifically targeting TRPCs (Hl-16258 and Hl-21915), PIEZ2 (Hl-13522) and EGFP (non-targeting negative control) were generated with a T7 RiboMAX Express RNAi System kit (Promega, Madison, WI) and diluted to RNase-free saline solution (RFSS). ..

Article Title: Carrier-freemRNA vaccine induces robust immunity against SARS-CoV-2 in mice and non-human primates without systemic reactogenicity
Article Snippet: .. The latter used in vitro transcribed RNA of the N protein-encoding gene (at 100, 101, 102, 103, 104, 106, and 108 copies/reaction); this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). ..

Negative Control:

Article Title: Supporting Information for The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins
Article Snippet: .. RNA interference (RNAi) Double-stranded RNA (dsRNA) specifically targeting TRPCs (Hl-16258 and Hl-21915), PIEZ2 (Hl-13522) and EGFP (non-targeting negative control) were generated with a T7 RiboMAX Express RNAi System kit (Promega, Madison, WI) and diluted to RNase-free saline solution (RFSS). ..

Saline:

Article Title: Supporting Information for The Holothuria leucospilota genome elucidates sacrificial organ expulsion and bioadhesive trap enriched with amyloid-patterned proteins
Article Snippet: .. RNA interference (RNAi) Double-stranded RNA (dsRNA) specifically targeting TRPCs (Hl-16258 and Hl-21915), PIEZ2 (Hl-13522) and EGFP (non-targeting negative control) were generated with a T7 RiboMAX Express RNAi System kit (Promega, Madison, WI) and diluted to RNase-free saline solution (RFSS). ..

In Vitro:

Article Title: Supporting Information for SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23
Article Snippet: .. For in vitro transcription, we used a Promega T7 RNA polymerase kit. .. Transcription reactions in 20 L used Transcription Optimized buffer (Promega) supplemented with 10 mM DTT, 0.5 L RNAsin, 2 L of rNTP nucleotide mix (5 mM ATP, 5 mM CTP, 1 mM GTP and 0.5 mM UTP), 1 L of 10 mM G-cap analog, 2.5 L of [α32P] UTP (10 mCi/L), 1 L of T7 RNA polymerase, and 2 g of DNA template.

Electrophoretic Mobility Shift Assay:

Article Title:
Article Snippet: .. Electrophoretic mobility shift assay The pF3A WG vectors containing the CDS of ANAC013, ANAC016 or ANAC017 fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol, but incubating the reaction for 3 h. Expression of proteins was validated by performing a western blot against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). ..

FLAG-tag:

Expressing:

Western Blot:

other:

Article Title:
Article Snippet: 148 For semi-in vivo pull-down EMF2-3Flag, SEAIR and its truncations were in vitro transcribed using 149 RiboMAX Large Scale RNA Production Systems-T7 (Promega) and were labelled with biotin using 150 the Biotin RNA Labeling Mix (Roche, Indianapolis).

Article Title: Listerin promotes cGAS protein degradation through ESCRT pathway to negatively regulate cGAS-mediated immune response
Article Snippet: Flag-TRIM27, Flag-Listerin, Flag-Listerin (ΔHEAT) and Flag-Listerin (ΔR) proteins were expressed with a TNT Quick Coupled Transcription/Translation System kit (Promega, L1171) according to the manufacturer’s protocol. cGAS protein expressed in E. coli BL21(DE3) was a gift from Deyu Zhu (ShanDong university, China).

Article Title: Mechanistic basis for potassium efflux driven activation of the human NLRP1 inflammasome
Article Snippet: In-vitro translation assay was performed using TNT® Quick Coupled Transcription/Translation System (Promega #L1170) following the manufacturer’s recommendations.

Review