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DIAGENODE DIAGNOSTICS 1-ml sonication buffer
1 Ml Sonication Buffer, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sonicated+buffer/1+ml+sonication+buffer/pmc09157090-202-8-16
Average 90 stars, based on 1 article reviews
1-ml sonication buffer - by Bioz Stars, 2026-10
90/100 stars

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Sonication:

Article Title: Inducible epigenome editing probes for the role of histone H3K4 methylation in Arabidopsis heat stress memory
Article Snippet: Cross-linking of samples was done under vacuum in ice-cold MC buffer with 1% (v/v) formaldehyde for 2 × 10 min. Chromatin was extracted as follows ( ): frozen tissue was ground to a fine powder, resuspended in 25-mL M1 buffer, filtered through Miracloth mesh (Merck Kenilworth, NJ, USA), washed five times in 5-mL M2 buffer, and once in 5-mL M3 buffer, with a centrifugation step at 4°C, 10 min, 1,000 g in between each wash. .. The resulting chromatin pellet was resuspended in 1-mL Sonication buffer and sheared with a Bioruptor Pico (Diagenode Denville, NJ, USA) for 17 cycles (30 s on/30 s off) on low-intensity settings. .. Equal chromatin amounts of chromatin from each sample were immunoprecipitated over night at 4°C using antibodies against Flag (F1804, Sigma-Aldrich), H3 (ab1791, Abcam), and H3K4me3 (ab8580, Abcam).



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