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hank's balanced saline solution (hbss  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hank's balanced saline solution (hbss
    Hank's Balanced Saline Solution (Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/solution/nct06063551-30-4-11
    Average 90 stars, based on 1 article reviews
    hank's balanced saline solution (hbss - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Injection:

    Article Title: Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury
    Article Snippet: .. Eighteen days after LPC injection, mice were anaesthetized with an i.p. injection of MMF (fentanyl (0.05 mg/kg)–midazolam (5 mg/kg)–medetomidine (1 mg/kg)) and transcardially perfused with 2 UI/mL Heparin (Heparin-Natrium-25000-ratiopharm®, PZN: 03029843) in HBSS (no calcium, no magnesium, GibcoTM, 14175129) for 3 min and 4% paraformaldehyde (PFA, EM Grade, Electron Microscopy Sciences, Cat. No. 15710, diluted in 10X PBS, InvitrogenTM (to a final concentration 1x), AM9624 and UltraPureTM Distilled Water, InvitrogenTM, 10977-035) for 5 min, before carefully removing the brain from the skull. .. Afterwards, the brains were fixed by submerging in 4% PFA for 6 h, followed by 14 h in 15% sucrose (Sigma-Aldrich, S0389 in UltraPureTM Distilled Water, InvitrogenTM, 10977-035) and 5 h in 30% sucrose.

    Article Title: Spatial Transcriptomics-correlated Electron Microscopy
    Article Snippet: .. 18 days after LPC injection, mice were anaesthetized with an i.p. injection of MMF (fentanyl (0.05 mg/kg)–midazolam (5 mg/kg)–medetomidine (1 mg/kg)) and transcardially perfused with 2 UI/mL Heparin (Heparin-Natrium-25000-ratiopharm®, PZN: 03029843) in HBSS (no calcium, no magnesium, GibcoTM, 14175129) for 3 min and 4 % paraformaldehyde (PFA, EM Grade, Electron Microscopy Sciences, Cat. No. 15710, diluted in 10X PBS, InvitrogenTM, AM9624 and UltraPureTM Distilled Water, InvitrogenTM, 10977-035) for 5 min, before carefully removing the brain from the skull. .. Afterwards, the brains were fixed by submergingin 4% PFA for 6 hours, followed by 14 hours in 15% sucrose (Sigma-Aldrich, S0389 in UltraPureTM Distilled Water, InvitrogenTM, 10977-035) and 5 hours in 30% sucrose.

    Electron Microscopy:

    Article Title: Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury
    Article Snippet: .. Eighteen days after LPC injection, mice were anaesthetized with an i.p. injection of MMF (fentanyl (0.05 mg/kg)–midazolam (5 mg/kg)–medetomidine (1 mg/kg)) and transcardially perfused with 2 UI/mL Heparin (Heparin-Natrium-25000-ratiopharm®, PZN: 03029843) in HBSS (no calcium, no magnesium, GibcoTM, 14175129) for 3 min and 4% paraformaldehyde (PFA, EM Grade, Electron Microscopy Sciences, Cat. No. 15710, diluted in 10X PBS, InvitrogenTM (to a final concentration 1x), AM9624 and UltraPureTM Distilled Water, InvitrogenTM, 10977-035) for 5 min, before carefully removing the brain from the skull. .. Afterwards, the brains were fixed by submerging in 4% PFA for 6 h, followed by 14 h in 15% sucrose (Sigma-Aldrich, S0389 in UltraPureTM Distilled Water, InvitrogenTM, 10977-035) and 5 h in 30% sucrose.

    Article Title: Spatial Transcriptomics-correlated Electron Microscopy
    Article Snippet: .. 18 days after LPC injection, mice were anaesthetized with an i.p. injection of MMF (fentanyl (0.05 mg/kg)–midazolam (5 mg/kg)–medetomidine (1 mg/kg)) and transcardially perfused with 2 UI/mL Heparin (Heparin-Natrium-25000-ratiopharm®, PZN: 03029843) in HBSS (no calcium, no magnesium, GibcoTM, 14175129) for 3 min and 4 % paraformaldehyde (PFA, EM Grade, Electron Microscopy Sciences, Cat. No. 15710, diluted in 10X PBS, InvitrogenTM, AM9624 and UltraPureTM Distilled Water, InvitrogenTM, 10977-035) for 5 min, before carefully removing the brain from the skull. .. Afterwards, the brains were fixed by submergingin 4% PFA for 6 hours, followed by 14 hours in 15% sucrose (Sigma-Aldrich, S0389 in UltraPureTM Distilled Water, InvitrogenTM, 10977-035) and 5 hours in 30% sucrose.

    Concentration Assay:

    Article Title: Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury
    Article Snippet: .. Eighteen days after LPC injection, mice were anaesthetized with an i.p. injection of MMF (fentanyl (0.05 mg/kg)–midazolam (5 mg/kg)–medetomidine (1 mg/kg)) and transcardially perfused with 2 UI/mL Heparin (Heparin-Natrium-25000-ratiopharm®, PZN: 03029843) in HBSS (no calcium, no magnesium, GibcoTM, 14175129) for 3 min and 4% paraformaldehyde (PFA, EM Grade, Electron Microscopy Sciences, Cat. No. 15710, diluted in 10X PBS, InvitrogenTM (to a final concentration 1x), AM9624 and UltraPureTM Distilled Water, InvitrogenTM, 10977-035) for 5 min, before carefully removing the brain from the skull. .. Afterwards, the brains were fixed by submerging in 4% PFA for 6 h, followed by 14 h in 15% sucrose (Sigma-Aldrich, S0389 in UltraPureTM Distilled Water, InvitrogenTM, 10977-035) and 5 h in 30% sucrose.

    Saline:

    Article Title: Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice
    Article Snippet: .. For biochemical and histological assessments, mice were terminally anesthetized with sodium pentobarbital diluted in saline (150 mg/kg, i.p.) and perfused with cold HBSS (cat no 14175129, Life Technologies, 3 ml/min). ..

    other:

    Article Title: Cryopreservation of Prepubertal Testicular Tissue for Preservation
    Article Snippet: Samples are collected into sterile Hank's balanced saline solution (HBSS; 14175-129; Life Technologies, Merelbeke, Belgium), and transported to Tissue Services for cryopreservation and storage.

    Gradient Centrifugation:

    Article Title: Preclinical model for the study of immune responses specific for a hepatic self-antigen
    Article Snippet: .. Briefly, after perfusion with HBSS 1X buffer (ThermoFisher Scientific #14175129), livers were digested with collagenase IV (Sigma-Aldrich #C5138) and NPCs enriched by Percoll (Sigma-Aldrich #GE17-0891-01) density gradient centrifugation and red blood cells lysis. ..

    Lysis:

    Article Title: Preclinical model for the study of immune responses specific for a hepatic self-antigen
    Article Snippet: .. Briefly, after perfusion with HBSS 1X buffer (ThermoFisher Scientific #14175129), livers were digested with collagenase IV (Sigma-Aldrich #C5138) and NPCs enriched by Percoll (Sigma-Aldrich #GE17-0891-01) density gradient centrifugation and red blood cells lysis. ..



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