Lysis:
Ubiquitin Proteomics:
Fluorescence:Article Title: Single-cell DNA Methylome and 3D Multi-omic Atlas of the Adult Mouse Brain
Article Snippet: snm3C/snmC-seq3 Beckman i7 Protocol – Ecker Lab MATERIALS- General reagents • FACS sorted single-nuclei into 384-well PCR plate • HyCloneTM HyPureTM Molecular Biology Grade (MB) Water (GE Life Sci. .. SH30538.08) • 200-Proof (100%) Ethanol (Koptec V1001) Collection of single nuclei by Fluorescence-activated Cell Sorting (FACS) • M-Digestion Buffer (Zymo D5021-9) • Proteinase K w/Storage Buffer (Zymo D3001-2-20) • CpG Methyltransferase (M.SssI) (NEB M0226) • Unmethylated Lambda DNA (Promega D1521) Bisulfite Conversion and Cleanup • CT Conversion Reagent (Zymo D5003-1) • M-Solubilization Buffer (Zymo D5021-7) • M-Dilution Buffer-Gold (Zymo D5006-2) • M-Reaction Buffer (Zymo D5021-8) • MagBead J (Zymo custom product D4100-3-100) • M-Binding Buffer (Zymo D5021-7) • M-Wash Buffer (Zymo D5040-4) • M-Desulphonation Buffer (Zymo D5040-5) • M-Elution Buffer (Zymo D5007-6) Random primers • Random Indexing Primer (IDT custom DNA oligo, standard desalted) Sera-Mag Solid Phase Reversible Immobilization (SPRI) beads • Sera-Mag SpeedBeads Magnetic Carboxylate Modified (GE Healthcare 5152105050250) • Poly(ethylene glycol) PEG 8000 (Sigma cat no. 89510-250G-F) • TE buffer pH=8.0 (Ambion AM9858) • 5M NaCl • 1M Tris-HCl pH=8.0 • 0.5M EDTA pH=8.0 • AMPure XP beads (Beckman Coulter cat no. A63881) • 100 bp DNA ladder (New England Biolabs cat no. N3231L) snmC-seq3 Library Preparation • Blue Buffer (10x) (Enzymatics P7010-HC-L) • Klenow Exo- (50U/μL) (Enzymatics P7010-HC-L) • Deoxynucleotide Solution Mix (10mM each dNTP) (NEB N0447L) • Exonuclease I (20U/μL) (Enzymatics X8010L) • Shrimp Alkaline Phosphatase (rSAP) (1U/μL) (NEB M0371L) • Sera-Mag SPRI beads • M-Elution Buffer (Zymo 5007-6) • EB buffer (Qiagen 19086) • Accel-NGS® AdaptaseTM Module (Swift Bio 330384) • P5 Indexing Primer (IDT custom DNA oligo, standard desalted) • P7 Indexing Primer (IDT custom DNA oligo, standard desalted) • KAPA HiFi HS RM (Kapa KK2602) • Qubit dsDNA BR Assay Kit (Thermo Fisher Q32850) Equipment • 384-Well Hardshell PCR Plate Clear, 20 Pcs/pk (Thermo Fisher 4483285) • 96-Well Hardshell PCR Plate GPLE, 20 Pcs/pk (Thermo Fisher 4483348) • MicroAmpTM Splash-Free 96-Well Base (Thermo Fisher 4312063) • Plateone® Deep 384-Well 200 μL Polypropylene Plate (USA-SCI. .. 1884-2410) • Reservoir Single Well 384 Bottom Low Profile, Clear (Axygen RES-SW384-LP) • Reservoir Single Well 96 Bottom High Profile, Clear (Axygen RES-SW96-HP) • Reservoir Single Well 96 Bottom Low Profile, Clear (Axygen RES-SW96-LP) • 15mL Centrifuge Tubes (Olympus 28-103) • 50mL Centrifuge Tubes (Olympus 28-106) • 1.7mL Microtube, Clear (Olympus 24-282LR) • Microamp Clear Adhesive Film, 100pc (Thermo Fisher 4306311) • Speedball Deluxe Soft Rubber Brayer, 4 inches (Statesville N.C.) • 37°C Incubator • 384-well and 96-well Compatible Thermocycler • DynaMagTM-96 Side Magnet (Thermo Fisher 12331D) • DynaMagTM-2 Magnet (Thermo Fisher 12321D) • 384-well Magnet Plate (Alpaqua A001222) • Allegra 25R Centrifuge with S5700 2x96 Swinging Bucket Rotor (Beckman Coulter 368954) • Axygen® 384-well tips, 45μL, Clear, Filtered, Sterile, Fine Point, SLAS Rack (Corning FXF-384- 45FP-R-S) • Axygen® 96-well tips, 200μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-200-R-S) • Axygen® 96-well tips, 30μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-50-R-S) • Beckman Coulter Biomek i7 dual-Multichannel with MC384 and MC96 Pipetting Heads REAGENT SETUP- Methylated λ DNA Dilute supplied SAM to 1600μM with Nuclease-free Water.
FACS:Article Title: Single-cell DNA Methylome and 3D Multi-omic Atlas of the Adult Mouse Brain
Article Snippet: snm3C/snmC-seq3 Beckman i7 Protocol – Ecker Lab MATERIALS- General reagents • FACS sorted single-nuclei into 384-well PCR plate • HyCloneTM HyPureTM Molecular Biology Grade (MB) Water (GE Life Sci. .. SH30538.08) • 200-Proof (100%) Ethanol (Koptec V1001) Collection of single nuclei by Fluorescence-activated Cell Sorting (FACS) • M-Digestion Buffer (Zymo D5021-9) • Proteinase K w/Storage Buffer (Zymo D3001-2-20) • CpG Methyltransferase (M.SssI) (NEB M0226) • Unmethylated Lambda DNA (Promega D1521) Bisulfite Conversion and Cleanup • CT Conversion Reagent (Zymo D5003-1) • M-Solubilization Buffer (Zymo D5021-7) • M-Dilution Buffer-Gold (Zymo D5006-2) • M-Reaction Buffer (Zymo D5021-8) • MagBead J (Zymo custom product D4100-3-100) • M-Binding Buffer (Zymo D5021-7) • M-Wash Buffer (Zymo D5040-4) • M-Desulphonation Buffer (Zymo D5040-5) • M-Elution Buffer (Zymo D5007-6) Random primers • Random Indexing Primer (IDT custom DNA oligo, standard desalted) Sera-Mag Solid Phase Reversible Immobilization (SPRI) beads • Sera-Mag SpeedBeads Magnetic Carboxylate Modified (GE Healthcare 5152105050250) • Poly(ethylene glycol) PEG 8000 (Sigma cat no. 89510-250G-F) • TE buffer pH=8.0 (Ambion AM9858) • 5M NaCl • 1M Tris-HCl pH=8.0 • 0.5M EDTA pH=8.0 • AMPure XP beads (Beckman Coulter cat no. A63881) • 100 bp DNA ladder (New England Biolabs cat no. N3231L) snmC-seq3 Library Preparation • Blue Buffer (10x) (Enzymatics P7010-HC-L) • Klenow Exo- (50U/μL) (Enzymatics P7010-HC-L) • Deoxynucleotide Solution Mix (10mM each dNTP) (NEB N0447L) • Exonuclease I (20U/μL) (Enzymatics X8010L) • Shrimp Alkaline Phosphatase (rSAP) (1U/μL) (NEB M0371L) • Sera-Mag SPRI beads • M-Elution Buffer (Zymo 5007-6) • EB buffer (Qiagen 19086) • Accel-NGS® AdaptaseTM Module (Swift Bio 330384) • P5 Indexing Primer (IDT custom DNA oligo, standard desalted) • P7 Indexing Primer (IDT custom DNA oligo, standard desalted) • KAPA HiFi HS RM (Kapa KK2602) • Qubit dsDNA BR Assay Kit (Thermo Fisher Q32850) Equipment • 384-Well Hardshell PCR Plate Clear, 20 Pcs/pk (Thermo Fisher 4483285) • 96-Well Hardshell PCR Plate GPLE, 20 Pcs/pk (Thermo Fisher 4483348) • MicroAmpTM Splash-Free 96-Well Base (Thermo Fisher 4312063) • Plateone® Deep 384-Well 200 μL Polypropylene Plate (USA-SCI. .. 1884-2410) • Reservoir Single Well 384 Bottom Low Profile, Clear (Axygen RES-SW384-LP) • Reservoir Single Well 96 Bottom High Profile, Clear (Axygen RES-SW96-HP) • Reservoir Single Well 96 Bottom Low Profile, Clear (Axygen RES-SW96-LP) • 15mL Centrifuge Tubes (Olympus 28-103) • 50mL Centrifuge Tubes (Olympus 28-106) • 1.7mL Microtube, Clear (Olympus 24-282LR) • Microamp Clear Adhesive Film, 100pc (Thermo Fisher 4306311) • Speedball Deluxe Soft Rubber Brayer, 4 inches (Statesville N.C.) • 37°C Incubator • 384-well and 96-well Compatible Thermocycler • DynaMagTM-96 Side Magnet (Thermo Fisher 12331D) • DynaMagTM-2 Magnet (Thermo Fisher 12321D) • 384-well Magnet Plate (Alpaqua A001222) • Allegra 25R Centrifuge with S5700 2x96 Swinging Bucket Rotor (Beckman Coulter 368954) • Axygen® 384-well tips, 45μL, Clear, Filtered, Sterile, Fine Point, SLAS Rack (Corning FXF-384- 45FP-R-S) • Axygen® 96-well tips, 200μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-200-R-S) • Axygen® 96-well tips, 30μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-50-R-S) • Beckman Coulter Biomek i7 dual-Multichannel with MC384 and MC96 Pipetting Heads REAGENT SETUP- Methylated λ DNA Dilute supplied SAM to 1600μM with Nuclease-free Water.
Lambda DNA Preparation:Article Title: Single-cell DNA Methylome and 3D Multi-omic Atlas of the Adult Mouse Brain
Article Snippet: snm3C/snmC-seq3 Beckman i7 Protocol – Ecker Lab MATERIALS- General reagents • FACS sorted single-nuclei into 384-well PCR plate • HyCloneTM HyPureTM Molecular Biology Grade (MB) Water (GE Life Sci. .. SH30538.08) • 200-Proof (100%) Ethanol (Koptec V1001) Collection of single nuclei by Fluorescence-activated Cell Sorting (FACS) • M-Digestion Buffer (Zymo D5021-9) • Proteinase K w/Storage Buffer (Zymo D3001-2-20) • CpG Methyltransferase (M.SssI) (NEB M0226) • Unmethylated Lambda DNA (Promega D1521) Bisulfite Conversion and Cleanup • CT Conversion Reagent (Zymo D5003-1) • M-Solubilization Buffer (Zymo D5021-7) • M-Dilution Buffer-Gold (Zymo D5006-2) • M-Reaction Buffer (Zymo D5021-8) • MagBead J (Zymo custom product D4100-3-100) • M-Binding Buffer (Zymo D5021-7) • M-Wash Buffer (Zymo D5040-4) • M-Desulphonation Buffer (Zymo D5040-5) • M-Elution Buffer (Zymo D5007-6) Random primers • Random Indexing Primer (IDT custom DNA oligo, standard desalted) Sera-Mag Solid Phase Reversible Immobilization (SPRI) beads • Sera-Mag SpeedBeads Magnetic Carboxylate Modified (GE Healthcare 5152105050250) • Poly(ethylene glycol) PEG 8000 (Sigma cat no. 89510-250G-F) • TE buffer pH=8.0 (Ambion AM9858) • 5M NaCl • 1M Tris-HCl pH=8.0 • 0.5M EDTA pH=8.0 • AMPure XP beads (Beckman Coulter cat no. A63881) • 100 bp DNA ladder (New England Biolabs cat no. N3231L) snmC-seq3 Library Preparation • Blue Buffer (10x) (Enzymatics P7010-HC-L) • Klenow Exo- (50U/μL) (Enzymatics P7010-HC-L) • Deoxynucleotide Solution Mix (10mM each dNTP) (NEB N0447L) • Exonuclease I (20U/μL) (Enzymatics X8010L) • Shrimp Alkaline Phosphatase (rSAP) (1U/μL) (NEB M0371L) • Sera-Mag SPRI beads • M-Elution Buffer (Zymo 5007-6) • EB buffer (Qiagen 19086) • Accel-NGS® AdaptaseTM Module (Swift Bio 330384) • P5 Indexing Primer (IDT custom DNA oligo, standard desalted) • P7 Indexing Primer (IDT custom DNA oligo, standard desalted) • KAPA HiFi HS RM (Kapa KK2602) • Qubit dsDNA BR Assay Kit (Thermo Fisher Q32850) Equipment • 384-Well Hardshell PCR Plate Clear, 20 Pcs/pk (Thermo Fisher 4483285) • 96-Well Hardshell PCR Plate GPLE, 20 Pcs/pk (Thermo Fisher 4483348) • MicroAmpTM Splash-Free 96-Well Base (Thermo Fisher 4312063) • Plateone® Deep 384-Well 200 μL Polypropylene Plate (USA-SCI. .. 1884-2410) • Reservoir Single Well 384 Bottom Low Profile, Clear (Axygen RES-SW384-LP) • Reservoir Single Well 96 Bottom High Profile, Clear (Axygen RES-SW96-HP) • Reservoir Single Well 96 Bottom Low Profile, Clear (Axygen RES-SW96-LP) • 15mL Centrifuge Tubes (Olympus 28-103) • 50mL Centrifuge Tubes (Olympus 28-106) • 1.7mL Microtube, Clear (Olympus 24-282LR) • Microamp Clear Adhesive Film, 100pc (Thermo Fisher 4306311) • Speedball Deluxe Soft Rubber Brayer, 4 inches (Statesville N.C.) • 37°C Incubator • 384-well and 96-well Compatible Thermocycler • DynaMagTM-96 Side Magnet (Thermo Fisher 12331D) • DynaMagTM-2 Magnet (Thermo Fisher 12321D) • 384-well Magnet Plate (Alpaqua A001222) • Allegra 25R Centrifuge with S5700 2x96 Swinging Bucket Rotor (Beckman Coulter 368954) • Axygen® 384-well tips, 45μL, Clear, Filtered, Sterile, Fine Point, SLAS Rack (Corning FXF-384- 45FP-R-S) • Axygen® 96-well tips, 200μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-200-R-S) • Axygen® 96-well tips, 30μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-50-R-S) • Beckman Coulter Biomek i7 dual-Multichannel with MC384 and MC96 Pipetting Heads REAGENT SETUP- Methylated λ DNA Dilute supplied SAM to 1600μM with Nuclease-free Water.
Modification:Article Title: Single-cell DNA Methylome and 3D Multi-omic Atlas of the Adult Mouse Brain
Article Snippet: snm3C/snmC-seq3 Beckman i7 Protocol – Ecker Lab MATERIALS- General reagents • FACS sorted single-nuclei into 384-well PCR plate • HyCloneTM HyPureTM Molecular Biology Grade (MB) Water (GE Life Sci. .. SH30538.08) • 200-Proof (100%) Ethanol (Koptec V1001) Collection of single nuclei by Fluorescence-activated Cell Sorting (FACS) • M-Digestion Buffer (Zymo D5021-9) • Proteinase K w/Storage Buffer (Zymo D3001-2-20) • CpG Methyltransferase (M.SssI) (NEB M0226) • Unmethylated Lambda DNA (Promega D1521) Bisulfite Conversion and Cleanup • CT Conversion Reagent (Zymo D5003-1) • M-Solubilization Buffer (Zymo D5021-7) • M-Dilution Buffer-Gold (Zymo D5006-2) • M-Reaction Buffer (Zymo D5021-8) • MagBead J (Zymo custom product D4100-3-100) • M-Binding Buffer (Zymo D5021-7) • M-Wash Buffer (Zymo D5040-4) • M-Desulphonation Buffer (Zymo D5040-5) • M-Elution Buffer (Zymo D5007-6) Random primers • Random Indexing Primer (IDT custom DNA oligo, standard desalted) Sera-Mag Solid Phase Reversible Immobilization (SPRI) beads • Sera-Mag SpeedBeads Magnetic Carboxylate Modified (GE Healthcare 5152105050250) • Poly(ethylene glycol) PEG 8000 (Sigma cat no. 89510-250G-F) • TE buffer pH=8.0 (Ambion AM9858) • 5M NaCl • 1M Tris-HCl pH=8.0 • 0.5M EDTA pH=8.0 • AMPure XP beads (Beckman Coulter cat no. A63881) • 100 bp DNA ladder (New England Biolabs cat no. N3231L) snmC-seq3 Library Preparation • Blue Buffer (10x) (Enzymatics P7010-HC-L) • Klenow Exo- (50U/μL) (Enzymatics P7010-HC-L) • Deoxynucleotide Solution Mix (10mM each dNTP) (NEB N0447L) • Exonuclease I (20U/μL) (Enzymatics X8010L) • Shrimp Alkaline Phosphatase (rSAP) (1U/μL) (NEB M0371L) • Sera-Mag SPRI beads • M-Elution Buffer (Zymo 5007-6) • EB buffer (Qiagen 19086) • Accel-NGS® AdaptaseTM Module (Swift Bio 330384) • P5 Indexing Primer (IDT custom DNA oligo, standard desalted) • P7 Indexing Primer (IDT custom DNA oligo, standard desalted) • KAPA HiFi HS RM (Kapa KK2602) • Qubit dsDNA BR Assay Kit (Thermo Fisher Q32850) Equipment • 384-Well Hardshell PCR Plate Clear, 20 Pcs/pk (Thermo Fisher 4483285) • 96-Well Hardshell PCR Plate GPLE, 20 Pcs/pk (Thermo Fisher 4483348) • MicroAmpTM Splash-Free 96-Well Base (Thermo Fisher 4312063) • Plateone® Deep 384-Well 200 μL Polypropylene Plate (USA-SCI. .. 1884-2410) • Reservoir Single Well 384 Bottom Low Profile, Clear (Axygen RES-SW384-LP) • Reservoir Single Well 96 Bottom High Profile, Clear (Axygen RES-SW96-HP) • Reservoir Single Well 96 Bottom Low Profile, Clear (Axygen RES-SW96-LP) • 15mL Centrifuge Tubes (Olympus 28-103) • 50mL Centrifuge Tubes (Olympus 28-106) • 1.7mL Microtube, Clear (Olympus 24-282LR) • Microamp Clear Adhesive Film, 100pc (Thermo Fisher 4306311) • Speedball Deluxe Soft Rubber Brayer, 4 inches (Statesville N.C.) • 37°C Incubator • 384-well and 96-well Compatible Thermocycler • DynaMagTM-96 Side Magnet (Thermo Fisher 12331D) • DynaMagTM-2 Magnet (Thermo Fisher 12321D) • 384-well Magnet Plate (Alpaqua A001222) • Allegra 25R Centrifuge with S5700 2x96 Swinging Bucket Rotor (Beckman Coulter 368954) • Axygen® 384-well tips, 45μL, Clear, Filtered, Sterile, Fine Point, SLAS Rack (Corning FXF-384- 45FP-R-S) • Axygen® 96-well tips, 200μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-200-R-S) • Axygen® 96-well tips, 30μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-50-R-S) • Beckman Coulter Biomek i7 dual-Multichannel with MC384 and MC96 Pipetting Heads REAGENT SETUP- Methylated λ DNA Dilute supplied SAM to 1600μM with Nuclease-free Water.
Polymerase Chain Reaction:Article Title: Single-cell DNA Methylome and 3D Multi-omic Atlas of the Adult Mouse Brain
Article Snippet: snm3C/snmC-seq3 Beckman i7 Protocol – Ecker Lab MATERIALS- General reagents • FACS sorted single-nuclei into 384-well PCR plate • HyCloneTM HyPureTM Molecular Biology Grade (MB) Water (GE Life Sci. .. SH30538.08) • 200-Proof (100%) Ethanol (Koptec V1001) Collection of single nuclei by Fluorescence-activated Cell Sorting (FACS) • M-Digestion Buffer (Zymo D5021-9) • Proteinase K w/Storage Buffer (Zymo D3001-2-20) • CpG Methyltransferase (M.SssI) (NEB M0226) • Unmethylated Lambda DNA (Promega D1521) Bisulfite Conversion and Cleanup • CT Conversion Reagent (Zymo D5003-1) • M-Solubilization Buffer (Zymo D5021-7) • M-Dilution Buffer-Gold (Zymo D5006-2) • M-Reaction Buffer (Zymo D5021-8) • MagBead J (Zymo custom product D4100-3-100) • M-Binding Buffer (Zymo D5021-7) • M-Wash Buffer (Zymo D5040-4) • M-Desulphonation Buffer (Zymo D5040-5) • M-Elution Buffer (Zymo D5007-6) Random primers • Random Indexing Primer (IDT custom DNA oligo, standard desalted) Sera-Mag Solid Phase Reversible Immobilization (SPRI) beads • Sera-Mag SpeedBeads Magnetic Carboxylate Modified (GE Healthcare 5152105050250) • Poly(ethylene glycol) PEG 8000 (Sigma cat no. 89510-250G-F) • TE buffer pH=8.0 (Ambion AM9858) • 5M NaCl • 1M Tris-HCl pH=8.0 • 0.5M EDTA pH=8.0 • AMPure XP beads (Beckman Coulter cat no. A63881) • 100 bp DNA ladder (New England Biolabs cat no. N3231L) snmC-seq3 Library Preparation • Blue Buffer (10x) (Enzymatics P7010-HC-L) • Klenow Exo- (50U/μL) (Enzymatics P7010-HC-L) • Deoxynucleotide Solution Mix (10mM each dNTP) (NEB N0447L) • Exonuclease I (20U/μL) (Enzymatics X8010L) • Shrimp Alkaline Phosphatase (rSAP) (1U/μL) (NEB M0371L) • Sera-Mag SPRI beads • M-Elution Buffer (Zymo 5007-6) • EB buffer (Qiagen 19086) • Accel-NGS® AdaptaseTM Module (Swift Bio 330384) • P5 Indexing Primer (IDT custom DNA oligo, standard desalted) • P7 Indexing Primer (IDT custom DNA oligo, standard desalted) • KAPA HiFi HS RM (Kapa KK2602) • Qubit dsDNA BR Assay Kit (Thermo Fisher Q32850) Equipment • 384-Well Hardshell PCR Plate Clear, 20 Pcs/pk (Thermo Fisher 4483285) • 96-Well Hardshell PCR Plate GPLE, 20 Pcs/pk (Thermo Fisher 4483348) • MicroAmpTM Splash-Free 96-Well Base (Thermo Fisher 4312063) • Plateone® Deep 384-Well 200 μL Polypropylene Plate (USA-SCI. .. 1884-2410) • Reservoir Single Well 384 Bottom Low Profile, Clear (Axygen RES-SW384-LP) • Reservoir Single Well 96 Bottom High Profile, Clear (Axygen RES-SW96-HP) • Reservoir Single Well 96 Bottom Low Profile, Clear (Axygen RES-SW96-LP) • 15mL Centrifuge Tubes (Olympus 28-103) • 50mL Centrifuge Tubes (Olympus 28-106) • 1.7mL Microtube, Clear (Olympus 24-282LR) • Microamp Clear Adhesive Film, 100pc (Thermo Fisher 4306311) • Speedball Deluxe Soft Rubber Brayer, 4 inches (Statesville N.C.) • 37°C Incubator • 384-well and 96-well Compatible Thermocycler • DynaMagTM-96 Side Magnet (Thermo Fisher 12331D) • DynaMagTM-2 Magnet (Thermo Fisher 12321D) • 384-well Magnet Plate (Alpaqua A001222) • Allegra 25R Centrifuge with S5700 2x96 Swinging Bucket Rotor (Beckman Coulter 368954) • Axygen® 384-well tips, 45μL, Clear, Filtered, Sterile, Fine Point, SLAS Rack (Corning FXF-384- 45FP-R-S) • Axygen® 96-well tips, 200μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-200-R-S) • Axygen® 96-well tips, 30μL, Clear, Filtered, Sterile, SLAS Rack (Corning FXF-50-R-S) • Beckman Coulter Biomek i7 dual-Multichannel with MC384 and MC96 Pipetting Heads REAGENT SETUP- Methylated λ DNA Dilute supplied SAM to 1600μM with Nuclease-free Water.
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