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Promega solubilization reagent
Solubilization Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+solution/solubilization+solution/pm19482900-54-8-10
Average 90 stars, based on 1 article reviews
solubilization reagent - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: Synthesis and functionalization of virus-mimicking cationic block copolymers with pathogen-associated carbohydrates as potential vaccine adjuvants
Article Snippet: .. Pentablock copolymers dissolved in HBSS Buffer were added at concentrations calculated from an NP ratio of 20 for 0.2 mg and 0.6 mg of DNA and incubated with the cells at 37 C for an additional 24 h. A 15 mL volume of the Promega tetrazolium salt reagent was added to each well, the 96 well plate was incubated for an additional 4 h and then a 100 mL volume of the Promega solubilization solution was added to each well. ..

Article Title: Oncogenic IL7R gain-of-function mutations in childhood T-cell acute lymphoblastic leukemia
Article Snippet: .. We added 8 ~l of MTT (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide; 5 mg/ml; Sigma) to each well and kept cells at 37 °C for 4 h, after which 100 ~l of solubilization solution (Promega) was added, and cells were incubated overnight at 37 °C. ..

Article Title: Inhibiting Janus Kinase 1 and BCL-2 to treat T cell acute lymphoblastic leukemia with IL7-Rα mutations
Article Snippet: 15 μL of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; 5 mg/ml; Sigma) was added to each well and incubated at 37°C for 4 hours. .. 100 μl solubilization solution (Promega) was added to each well, and cells were incubated over night at 37°C. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide.
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 ◦ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 ◦ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 ◦ C. The absorbance was measured at 570 nm. ..

Article Title: Macrophages and Natural Killers Degrade α-Synuclein Aggregates.
Article Snippet: .. Next, solubilization solution (Promega, Madison, Wisconsin) was added and incubated for 1 h at 37 °C under 5% CO2. .. Absorption measurements were made in a plate reader (Tecan, Man̈nedorf, Switzerland) at 570 nm.

Article Title: Macrophages and Natural Killers Degrade α-Synuclein Aggregates
Article Snippet: .. Next, solubilization solution (Promega, Madison, Wisconsin) was added and incubated for 1 h at 37 °C under 5% CO 2 . .. Absorption measurements were made in a plate reader (Tecan, Männedorf, Switzerland) at 570 nm.

Article Title: The Anti-Aging and Tumor Suppressor Protein Klotho Enhances Differentiation of a Human Oligodendrocytic Hybrid Cell Line
Article Snippet: .. After 24 h, the medium was replaced with fresh DMEM with 10 % FBS or serum-free DMEM and cells were treated with recombinant Klotho at 0.4 μg/mL for 48 h. Following treatment, 15 μL of 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (Promega) were added to cells, incubated for 3 h at 37 °C, then quenched with 100 μL of solubilization solution from the Promega kit for 30 min at room temperature. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. The absorbance was measured at 570 nm. ..

MTT Assay:

Article Title: Oncogenic IL7R gain-of-function mutations in childhood T-cell acute lymphoblastic leukemia
Article Snippet: .. We added 8 ~l of MTT (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide; 5 mg/ml; Sigma) to each well and kept cells at 37 °C for 4 h, after which 100 ~l of solubilization solution (Promega) was added, and cells were incubated overnight at 37 °C. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide.
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 ◦ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 ◦ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 ◦ C. The absorbance was measured at 570 nm. ..

Article Title: The Anti-Aging and Tumor Suppressor Protein Klotho Enhances Differentiation of a Human Oligodendrocytic Hybrid Cell Line
Article Snippet: .. After 24 h, the medium was replaced with fresh DMEM with 10 % FBS or serum-free DMEM and cells were treated with recombinant Klotho at 0.4 μg/mL for 48 h. Following treatment, 15 μL of 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (Promega) were added to cells, incubated for 3 h at 37 °C, then quenched with 100 μL of solubilization solution from the Promega kit for 30 min at room temperature. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. The absorbance was measured at 570 nm. ..

Variant Assay:

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide.
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 ◦ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 ◦ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 ◦ C. The absorbance was measured at 570 nm. ..

Article Title: Solid-phase synthesis and pathological evaluation of pyroglutamate amyloid-β 3-42 peptide
Article Snippet: .. Each Aβ variant (10 μM) was prepared in starvation medium (0.5% foetal bovine serum and 1% penicillin in DMEM) and treated on the plate for 24 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. 15 μL of MTT reagent (Promega, USA) was added to each well and incubated for additional 4 h at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. Finally, 100 μl of solubilization solution (Promega, USA) was added for 30 min at 37 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^\circ$$\end{document} ∘ C. The absorbance was measured at 570 nm. ..

Recombinant:

Article Title: The Anti-Aging and Tumor Suppressor Protein Klotho Enhances Differentiation of a Human Oligodendrocytic Hybrid Cell Line
Article Snippet: .. After 24 h, the medium was replaced with fresh DMEM with 10 % FBS or serum-free DMEM and cells were treated with recombinant Klotho at 0.4 μg/mL for 48 h. Following treatment, 15 μL of 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye (Promega) were added to cells, incubated for 3 h at 37 °C, then quenched with 100 μL of solubilization solution from the Promega kit for 30 min at room temperature. ..



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