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Structured Review

Anatrace solubilization buffer
Solubilization Buffer, supplied by Anatrace, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+buffer/buffer+solubilization/pm41813902-449-7-15
Average 86 stars, based on 1 article reviews
solubilization buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Membrane:

Article Title: Blood-derived lysophospholipid sustains hepatic phospholipids and fat storage necessary for hepatoprotection in overnutrition
Article Snippet: .. The membrane pellet was resuspended in solubilization buffer (20 mM HEPES, pH 7.50, 200 mM NaCl, 1% Fos-choline 13; F310, Anatrace) supplemented with protease inhibitor cocktail (Roche) and incubated overnight at 4°C. .. Protein concentration was determined using the BCA Protein Assay Kit (23227, Thermo Scientific).

Article Title: The dynamic basis of G-protein recognition and activation by a GPCR.
Article Snippet: G-protein-coupled receptor (GPCR) signalling occurs through heterotrimeric G proteins, whose selective activation leads to distinct cellular outcomes.. Although more than 200 GPCR–G protein complex structures have been determined, these static snapshots provide limited insight into the dynamics of G-protein association and dissociation.. Here we present cryo-electron microscopy structures of human neurotensin receptor type 1 (NTSR1) with minimally modified Go and Gq, showing how the receptor’s intracellular surface dynamically rearranges to accommodate each G-protein subtype.

Protease Inhibitor:

Article Title: Blood-derived lysophospholipid sustains hepatic phospholipids and fat storage necessary for hepatoprotection in overnutrition
Article Snippet: .. The membrane pellet was resuspended in solubilization buffer (20 mM HEPES, pH 7.50, 200 mM NaCl, 1% Fos-choline 13; F310, Anatrace) supplemented with protease inhibitor cocktail (Roche) and incubated overnight at 4°C. .. Protein concentration was determined using the BCA Protein Assay Kit (23227, Thermo Scientific).

Article Title: Structure-guided development of a potent human B 0 AT1 inhibitor effective in a mouse model of phenylketonuria
Article Snippet: .. To prepare the B 0 AT1–ACE2 complex, the harvested cells were solubilized for 2 h at 4 °C in solubilization buffer (25 mM Tris-HCl [pH 8.0], 150 mM NaCl, 1% GDN [Anatrace], and protease inhibitor cocktail). .. After ultracentrifugation (20,000 × g , 45 min, 4 °C) and filtering through a syringe filter (0.45 μm pore size), the supernatant was incubated with M2 Anti-FLAG Affinity Resin (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris-HCl [pH 8.0], 150 mM NaCl, 0.02% GDN) and incubated for 2 h at 4 °C.

Incubation:

Article Title: Blood-derived lysophospholipid sustains hepatic phospholipids and fat storage necessary for hepatoprotection in overnutrition
Article Snippet: .. The membrane pellet was resuspended in solubilization buffer (20 mM HEPES, pH 7.50, 200 mM NaCl, 1% Fos-choline 13; F310, Anatrace) supplemented with protease inhibitor cocktail (Roche) and incubated overnight at 4°C. .. Protein concentration was determined using the BCA Protein Assay Kit (23227, Thermo Scientific).

Article Title: Structural basis for ligand promiscuity and high signaling activity of Kaposi's Sarcoma-associated Herpesvirus-encoded GPCR.
Article Snippet: .. The resuspended mixture was then incubated with 100mL of 2× solubilization buffer (20mM HEPES pH 8.0, 100mM NaCl, 5mM MgCl2, 5mM imidazole, 2% sodium cholate, 0.1% n-Dodecyl-β-D-Maltoside (DDM, Anatrace, D310), 4 μL calf intestinal alkaline phosphatase (CIP, NEB M0525), 5mM 2-mercaptoethanol, 10 μM GDP) under nutation for 1 h. Following solubilization, the mixture was centrifuged again at 180,000× g for 30min, and the supernatant was incubated with NiNTA resin at 4 °C for 2 hwith rotation. ..

Chromatography:

Article Title: The dynamic basis of G-protein recognition and activation by a GPCR.
Article Snippet: G-protein-coupled receptor (GPCR) signalling occurs through heterotrimeric G proteins, whose selective activation leads to distinct cellular outcomes.. Although more than 200 GPCR–G protein complex structures have been determined, these static snapshots provide limited insight into the dynamics of G-protein association and dissociation.. Here we present cryo-electron microscopy structures of human neurotensin receptor type 1 (NTSR1) with minimally modified Go and Gq, showing how the receptor’s intracellular surface dynamically rearranges to accommodate each G-protein subtype.



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