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wt vol n dodecyl β d maltoside ddm anatrace  (Anatrace)

 
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    Structured Review

    Anatrace wt vol n dodecyl β d maltoside ddm anatrace
    Wt Vol N Dodecyl β D Maltoside Ddm Anatrace, supplied by Anatrace, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/solubilization+buffer+ddm/d+dodecyl+maltoside+n+%CE%B2/pmc12915331-106-21-24
    Average 86 stars, based on 1 article reviews
    wt vol n dodecyl β d maltoside ddm anatrace - by Bioz Stars, 2026-11
    86/100 stars

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    Membrane:

    Article Title: Subtype-dependent arrestin engagement of the metabotropic glutamate receptors.
    Article Snippet: Ni-NTA resin (Qiagen) was further used AR TI CL E IN P RE SS to remove the PreScission protease and cleaved His tag. .. The supernatant was discarded by ultracentrifugation at 100,000g for 30 min, and the cell membrane pellets were resuspended in buffer B supplemented with 1% (w/v) n-dodecyl-β-Dmaltopyranoside (DDM) (Anatrace), 0.2% (w/v) cholesterol hemisuccinate (CHS) (SigmaAldrich), 50 μM LSN2463359 and 0.2% (w/v) sodium cholate. .. After incubating at 4 °C for 3 h, the supernatant was isolated by ultracentrifugation at 100,000g for 30 min and then incubated with Strep-Tactin resin (IBA Lifesciences) at 4 °C overnight.

    Article Title: pH-mediated activation of the lysosomal arginine sensor SLC38A9.
    Article Snippet: .. To purify SLC38A9 protein and its variants, the membrane fraction was thawed and solubilized with 2% ndodecyl-β-D-maltopyranoside (DDM; Anatrace, Maumee, OH, USA), 0.2% Cholesteryl Hemisuccinate Tris salt (CHS; Anatrace) in 20 mM Tris pH 8.0, and 150mM NaCl with 10% glycerol for 2 h at 4 °C. .. Following another ultra-centrifugation at 130 000× g for 45 min, the supernatant was loaded onto TALON Metal Affinity Resin (Clontech, San Jose, CA, USA) and incubated at 4 °C overnight.

    other:

    Article Title: Defining state-selective lipid binding to brain GPCRs - introducing REVEAL
    Article Snippet: n-dodecyl β-D-maltoside (DDM) and n-decyl-β-D-maltoside (DM) were purchased from Anatrace.

    Centrifugation:

    Article Title: Structure-function analysis of the lithium-ion selectivity of the voltage-gated sodium channel
    Article Snippet: .. Membranes were collected by centrifugation (100,000 × g , 1 h, 4°C) and solubilized by homogenization in TBS buffer containing 30 mM n-dodecyl-β-D-maltoside (DDM, Anatrace). .. After centrifugation (40,000 × g , 30 min, 4°C), the supernatant was loaded onto a HIS-Select Cobalt Affinity Gel column (Sigma-Aldrich).

    Homogenization:

    Article Title: Structure-function analysis of the lithium-ion selectivity of the voltage-gated sodium channel
    Article Snippet: .. Membranes were collected by centrifugation (100,000 × g , 1 h, 4°C) and solubilized by homogenization in TBS buffer containing 30 mM n-dodecyl-β-D-maltoside (DDM, Anatrace). .. After centrifugation (40,000 × g , 30 min, 4°C), the supernatant was loaded onto a HIS-Select Cobalt Affinity Gel column (Sigma-Aldrich).

    Transcranial Magnetic Stimulation:

    Article Title: Structure-function analysis of the lithium-ion selectivity of the voltage-gated sodium channel
    Article Snippet: .. Membranes were collected by centrifugation (100,000 × g , 1 h, 4°C) and solubilized by homogenization in TBS buffer containing 30 mM n-dodecyl-β-D-maltoside (DDM, Anatrace). .. After centrifugation (40,000 × g , 30 min, 4°C), the supernatant was loaded onto a HIS-Select Cobalt Affinity Gel column (Sigma-Aldrich).

    Protease Inhibitor:

    Article Title: Structural and dynamic studies uncover a distinct allosteric modulatory site at the µ-opioid receptor.
    Article Snippet: .. Protein complexes were extracted in solubilization buffer containing 20 mM HEPES pH 7.5, 50 mM NaCl, 5 mM MgCl2, 0.3 mM TCEP, 1% (w/v) n-dodecyl-β-dmaltopyranoside (DDM; Anatrace), 0.2% (w/v) cholesteryl hemisuccinate (CHS; Anatrace) and home-made protease inhibitor cocktail. ..

    Lysis:

    Article Title: Structure and druggable conformation of the homodimeric lipoprotein transporter of Acinetobacter baumannii.
    Article Snippet: .. The total membranes were resuspended in lysis buffer with 1% (w/v) n-dodecyl-β-dmaltopyranoside (DDM; Anatrace) for 1 h at 4°C. .. The solubilized membranes were affinity-purified by loading onto TALONTM cobalt affinity resin (Takara Bio).



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