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r5020 (Revvity)

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Revvity r5020
R5020, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid/Promegestone+Solid/pmc03674071-368-10-11
Average 91 stars, based on 4 article reviews

r5020 - by Bioz Stars, 2026-10

91/100 stars

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Binding Assay:

Article Title: Longitudinal Noninvasive Imaging of Progesterone Receptor as a Predictive Biomarker of Tumor Responsiveness to Estrogen Deprivation Therapy
Article Snippet: .. Promegestone (R5020; PerkinElmer) has high affinity for the progesterone receptor (PgR) (relative binding affinity [RBA] = 100), but does not bind the glucocorticoid receptor (GR) at significant levels (RBA = 2.6) ( 18 ). .. Dexamethasone (Dex; MP Biomedicals) has high affinity for GR (RBA = 100), but does not have any appreciable affinity for PgR (RBA = 0.21) ( 19 ).

Concentration Assay:

Article Title: Progesterone receptor isoforms, agonists and antagonists differentially reprogram estrogen signaling
Article Snippet: .. Estradiol (Sigma #E8875-250MG) and R5020 (PerkinElmer #NLP004005MG) dissolved in ethanol (vehicle) were used at a final concentration of 10 nM for all the experiments except the cell viability assays. ..

other:

Article Title: Progesterone induces progesterone receptor gene (PGR) expression via rapid activation of protein kinase pathways required for cooperative estrogen receptor alpha (ER) and progesterone receptor (PR) genomic action at ER/PR target genes
Article Snippet: Cells were treated with the following reagents (when applicable and at indicated doses): R5020 (Perkin Elmer, #NLP004005MG), β-estradiol (E2) (Sigma, #E8875), RU486 (Sigma, #M8046), ICI 182,780 (Tocris, #1047), U0126 (U0) (EMD Millipore, 19-147), and {"type":"entrez-nucleotide","attrs":{"text":"LY294002","term_id":"1257998346","term_text":"LY294002"}} LY294002 (EMD Millipore, #440202).

Control:

Article Title: A molecular toolbox to study progesterone receptor signaling
Article Snippet: .. On the next day, cells were treated with either EtOH (control), 20nM R5020 (Perkin Elmer, #NLP004005MG), or 20nM R5020 and 100nM RU486 (Sigma Aldrich, #475838) in fresh medium. ..

Negative Control:

Article Title: A molecular toolbox to study progesterone receptor signaling
Article Snippet: .. The next day, cells were treated with 20nM R5020 (Promegestone, Perkin Elmer, #NLP004005MG), or 20nM R5020 and 100nM RU486 (Mifestrone, Sigma Aldrich, #475838) dissolved in ethanol, which was also taken along as a negative control. .. Two hours after treatment, the cells were fixed in 4% paraformaldehyde (Alfa Aesar, #43368) in PBS for 15 minutes at room temperature (RT) and washed with HBSS (Thermo Fisher, #11550456) three times.