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zymo solid tissue buffer  (Zymo Research)


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    Structured Review

    Zymo Research zymo solid tissue buffer
    Zymo Solid Tissue Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/solid+tissue+buffer/Solid+Tissue+Buffer+(Blue)/pmc13084153-97-23-23
    Average 94 stars, based on 20 article reviews
    zymo solid tissue buffer - by Bioz Stars, 2026-09
    94/100 stars

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    Lysis:

    Article Title: Distinct oral-associated gastric microbiota and Helicobacter pylori communities for spatial microbial heterogeneity in gastric cancer
    Article Snippet: The frozen tissues were soaked in DNA/RNA Shield Reagent (ZYMO Research) prior to DNA extraction. .. The dissolved tissues were mixed with Solid Tissue Buffer from a Quick-DNA Miniprep Plus Kit (D4069, ZYMO Research), digested with Proteinase K, and disrupted by beads beating (ZR BashingBead Lysis Tube) as previously described ( ). .. The dental plaque samples were mixed with BioFluid and Cell Buffer from a Quick-DNA Miniprep Plus Kit (D4069, ZYMO Research).

    Homogenization:

    Article Title: Comparative distribution and population genetics of bisexual and parthenogenetic Asian longhorned tick (Haemaphysalis longicornis) in the Republic of Korea.
    Article Snippet: .. Genomic DNA was extracted from each individual following whole-body homogenization in a FastPrep® 2 ml tube (MP Biomedicals, USA) with four 2.8 mm stainless steel beads (Innogenetech, Gimpo, Korea) in a mixture of solid tissue buffer and proteinase K. A total of 200 μl of the homogenate was used to extract the genomic DNA using a Quick-DNATM Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instructions. .. The purity and concentration of the genomic DNA were measured using a nanophotometer (Implen, Germany).

    Respiration Assay:

    Article Title: Chronic exposure to low levels of glyphosate and metals induces kidney dysfunction.
    Article Snippet: Chronic kidney disease (CKD) affects ~15% of U.S. adults and over 840 million people worldwide.. Environmental contaminants, including pesticides and metals, are increasingly recognized as disease contributors, yet mechanisms and consequences of long-term, low-level mixture exposures remain poorly defined.. Our prior work identified glyphosate and metals (cadmium, arsenic, lead, vanadium) in drinking water from agricultural regions with high CKD prevalence and showed that early-life co-exposures disrupt kidney development.

    Article Title: Chronic exposure to low levels of glyphosate and metals induces kidney dysfunction
    Article Snippet: .. Tissues used in the respiration assay were thawed after storage in DNA/RNA Shield (Zymo Research, Irvine, California), lysed with a hand-held homogenizer in Zymo Solid Tissue Buffer, and whole gDNA purified using Zymo DNA Mini+ 96-well kit (Zymo Research). .. FAM-mt-nd1 and SUN-nuc-actin1b (PrimeTime qPCR Probes, IDT, Coralville, Iowa) were used to target mtDNA and nuclear DNA, respectively.

    Purification:

    Article Title: Chronic exposure to low levels of glyphosate and metals induces kidney dysfunction.
    Article Snippet: Chronic kidney disease (CKD) affects ~15% of U.S. adults and over 840 million people worldwide.. Environmental contaminants, including pesticides and metals, are increasingly recognized as disease contributors, yet mechanisms and consequences of long-term, low-level mixture exposures remain poorly defined.. Our prior work identified glyphosate and metals (cadmium, arsenic, lead, vanadium) in drinking water from agricultural regions with high CKD prevalence and showed that early-life co-exposures disrupt kidney development.

    Article Title: Chronic exposure to low levels of glyphosate and metals induces kidney dysfunction
    Article Snippet: .. Tissues used in the respiration assay were thawed after storage in DNA/RNA Shield (Zymo Research, Irvine, California), lysed with a hand-held homogenizer in Zymo Solid Tissue Buffer, and whole gDNA purified using Zymo DNA Mini+ 96-well kit (Zymo Research). .. FAM-mt-nd1 and SUN-nuc-actin1b (PrimeTime qPCR Probes, IDT, Coralville, Iowa) were used to target mtDNA and nuclear DNA, respectively.

    Sequencing:

    Article Title: Eco-genomic analysis uncovers precision-conservation targets for the western Pacific’s southernmost salmonid
    Article Snippet: .. For genome sequencing, 30-50 mg of muscle tissue was lysed with proteinase K and Biofluid & Solid Tissue Buffer from Quick-DNATM HMW MagBead Kit (Catalog No. D6060, Zymo Research Corp., USA), following the manufacturer’s instructions. .. For ddRADseq, 2-10 mg of fin tissue was lysed with proteinase K and BL2 Buffer from MagPurix Tissue DNA Extraction Kit (Catalog No. ZP02004, Zinexts Life Science Corp., Taiwan), following the manufacturer’s instructions.

    Binding Assay:

    Article Title: Genome‐Wide Association Study (GWAS) for Linear Body Measurements of Sussex Cattle ( Bos taurus ) at Weaning and Yearling Age in South Africa
    Article Snippet: The DNA was obtained using the Zymo Research Quick‐DNA Miniprep Plus Kit (Zymo Research, USA) following the manufacturer's protocol and quantified using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific). .. Briefly, ∼25 hair root samples were placed in a micro‐centrifuge tube and a solution of 95 μL water, 95 μL solid tissue buffer and 10 μL proteinase K was added before mixing and incubating at 55°C for 1–3 h. Two volumes of genomic binding buffer were then added to the supernatant and mixed for 15 s. The mixture was transferred to a Zymo‐Spin llC‐XLR column in a collection tube and centrifuged at ≥ 12,000 × g for 1 min four times. ..



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