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iscript cdna synthesis kit  (Bio-Rad)


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    Structured Review

    Bio-Rad iscript cdna synthesis kit

    Iscript Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 48562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software+program+for+dna+sequencing/iScript+cDNA+Synthesis+Kit/pmc08880996-63-5-10
    Average 99 stars, based on 48562 article reviews
    iscript cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Induction of osteogenesis by bone-targeted Notch activation"

    Article Title: Induction of osteogenesis by bone-targeted Notch activation

    Journal: eLife

    doi: 10.7554/eLife.60183


    Figure Legend Snippet:

    Techniques Used: Recombinant, Variant Assay, Plasmid Preparation, Sequencing, Control, Gene Expression, Bicinchoninic Acid Protein Assay, cDNA Synthesis, Amplification, Magnetic Beads, Protease Inhibitor, Western Blot, Software, Staining

    Related Articles

    Synthesized:

    Article Title: Urinary KIM-1 Transcript Detectability in Workers Chronically Exposed to Arsenic and Antimony in an Industrial Area of Central Chile: A Cross-Sectional Pilot Study.
    Article Snippet: Background: Chronic arsenic (As) and antimony (Sb) exposure causes renal toxicity in experimental models, but early molecular indicators of tubular stress in humans remain uncharacterised.. Jo ur na l P re -p ro of Methods: We assessed 12 male workers from Puchuncaví, Chile.. Urinary and blood As and Sb were quantified by hydride-generation atomic fluorescence spectrometry (HG-AFS).

    Article Title: A Combination of Ursolic Acid and Oleanolic Acid Disrupts Bioenergetic Homeostasis in Prostate Cancer and Inhibits Cancer Growth by Cytotoxic Autophagy.
    Article Snippet: .. Total RNA from treated and control cells was isolated using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA) and cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad Laboratories) as per manufacturer protocol. .. The qPCR was performed on Applied Biosystems QuantStudio 3 Real-Time PCR system using the Universal SYBR Green Fast qPCR mix (Bio-Rad Laboratories) as described before [57].

    Article Title: The homologous GRAS transcription factors RAM1 and RAD1 coordinate shared and separate gene networks to regulate arbuscular mycorrhiza symbiosis.
    Article Snippet: Genomic DNA was removed using DNase I (AMPD1, Sigma-Aldrich) or TURBODNA-freeTM Kit (Ambion). .. cDNAwas synthesized using the iScriptTM cDNA Synthesis Kit (Bio-Rad), and qPCR was performed with primers shown in Table S1 on a Bio-Rad CFX 384 qPCR system or a Roche LIGHTCYCLER 480 system using SybrMaster (Jena Bioscience, Jena, Germany). (a) (b) (c) (d) (e) (f) 2026 The Author(s). ..

    cDNA Synthesis:

    Article Title: Urinary KIM-1 Transcript Detectability in Workers Chronically Exposed to Arsenic and Antimony in an Industrial Area of Central Chile: A Cross-Sectional Pilot Study.
    Article Snippet: Background: Chronic arsenic (As) and antimony (Sb) exposure causes renal toxicity in experimental models, but early molecular indicators of tubular stress in humans remain uncharacterised.. Jo ur na l P re -p ro of Methods: We assessed 12 male workers from Puchuncaví, Chile.. Urinary and blood As and Sb were quantified by hydride-generation atomic fluorescence spectrometry (HG-AFS).

    Article Title: Heat-Inactivated Lactiplantibacillus plantarum (RSB11) as a Multifunctional Postbiotic: Anti-Inflammatory Effects Compared with Live RSB11 Across Multiple Human Cell Models.
    Article Snippet: .. Unless specified, molecular biology reagents (RNeasy Mini Kit (Qiagen, Hilden, Germany), iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA), TaqMan master mix (Thermo Fisher Scientific, Waltham, MA, USA) and ELISA kits (R&D Systems, Minneapolis, MN, USA) were from standard vendors. ..

    Article Title: A Combination of Ursolic Acid and Oleanolic Acid Disrupts Bioenergetic Homeostasis in Prostate Cancer and Inhibits Cancer Growth by Cytotoxic Autophagy.
    Article Snippet: .. Total RNA from treated and control cells was isolated using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA) and cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad Laboratories) as per manufacturer protocol. .. The qPCR was performed on Applied Biosystems QuantStudio 3 Real-Time PCR system using the Universal SYBR Green Fast qPCR mix (Bio-Rad Laboratories) as described before [57].

    Article Title: Hesperidin Attenuates Vincristine‐Induced Nephrotoxicity via Nrf2/HO‐1 and PI3K/Akt/mTOR Signaling
    Article Snippet: The RNA concentrations were standardized across samples, and the RNA purity and quantity were assessed using a NanoDrop spectrophotometer (BioTek Instruments, EPOCH, USA). .. In accordance with the manufacturer's instructions, the iScript cDNA Synthesis Kit (Bio‐Rad, USA) was employed to generate complementary DNA (cDNA). .. The qRT‐PCR was conducted using a Rotor‐Gene Q system (Qiagen, Germany) and iTaq Universal SYBR Green Supermix (Bio‐Rad, USA).

    Article Title: Senescent Fibroblasts Impair B Cell Proliferation via Cell–Cell Contact
    Article Snippet: RNA from 0.5‐1 × 10 7 cells was purified using the RNeasy Mini Kit (Qiagen, cat. no. 74104) following the manufacturer's instructions. .. RNA concentration was quantified using a NanoDrop One (Thermo Scientific) and 1 μg RNA converted into cDNA using the iScript cDNA Synthesis Kit (Bio Rad, #1708891) and stored at −20°C until use. .. Taqman primer‐probes were used for qPCR: CDKN2A (Hs00923894_m1), CDKN1A (Hs00355782_m1) and IL6 (Hs00174131_m1) were assayed alongside VAMP7 (Hs00194568_m1) and L3MBT2L (Hs01002034_m1) as housekeeper genes.

    Article Title: Andrographolide Promotes Post-Ischemic Endothelial Angiogenic Activity via IL-10-Mediated M2-Like Microglia-Endothelial Crosstalk Involving Endothelial VEGFR2 Upregulation.
    Article Snippet: The RNA was quantified using a spectrophotometer (Denovix (Wilmington, DE, USA)). .. RNA (1000 ng) was used as a template and was subjected to reverse transcription using an iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). .. Real-time quantitative PCR (RT-qPCR) was performed in duplicate for each sample using a Bio-Rad Real-Time PCR system (CFX96 Optics Module BioRad).

    Article Title: Radiofrequency Radiation Alters Redox Homeostasis, ATP Status, and Apoptosis-Associated Responses in Human Ovarian Epithelial Cells.
    Article Snippet: RNA quantity and quality were assessed via NanoDrop spectrophotometry. .. cDNA synthesis from 500 ng total RNA utilized the Bio-Rad iScript cDNA Synthesis Kit in a 10 μL reaction volume comprising 2 μL 5X iScript reaction mix, 0.5 μL iScript reverse transcriptase, RNA template, and nuclease-free water. ..

    Article Title: The homologous GRAS transcription factors RAM1 and RAD1 coordinate shared and separate gene networks to regulate arbuscular mycorrhiza symbiosis.
    Article Snippet: Genomic DNA was removed using DNase I (AMPD1, Sigma-Aldrich) or TURBODNA-freeTM Kit (Ambion). .. cDNAwas synthesized using the iScriptTM cDNA Synthesis Kit (Bio-Rad), and qPCR was performed with primers shown in Table S1 on a Bio-Rad CFX 384 qPCR system or a Roche LIGHTCYCLER 480 system using SybrMaster (Jena Bioscience, Jena, Germany). (a) (b) (c) (d) (e) (f) 2026 The Author(s). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Heat-Inactivated Lactiplantibacillus plantarum (RSB11) as a Multifunctional Postbiotic: Anti-Inflammatory Effects Compared with Live RSB11 Across Multiple Human Cell Models.
    Article Snippet: .. Unless specified, molecular biology reagents (RNeasy Mini Kit (Qiagen, Hilden, Germany), iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA), TaqMan master mix (Thermo Fisher Scientific, Waltham, MA, USA) and ELISA kits (R&D Systems, Minneapolis, MN, USA) were from standard vendors. ..

    Control:

    Article Title: A Combination of Ursolic Acid and Oleanolic Acid Disrupts Bioenergetic Homeostasis in Prostate Cancer and Inhibits Cancer Growth by Cytotoxic Autophagy.
    Article Snippet: .. Total RNA from treated and control cells was isolated using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA) and cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad Laboratories) as per manufacturer protocol. .. The qPCR was performed on Applied Biosystems QuantStudio 3 Real-Time PCR system using the Universal SYBR Green Fast qPCR mix (Bio-Rad Laboratories) as described before [57].

    Isolation:

    Article Title: A Combination of Ursolic Acid and Oleanolic Acid Disrupts Bioenergetic Homeostasis in Prostate Cancer and Inhibits Cancer Growth by Cytotoxic Autophagy.
    Article Snippet: .. Total RNA from treated and control cells was isolated using Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA) and cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad Laboratories) as per manufacturer protocol. .. The qPCR was performed on Applied Biosystems QuantStudio 3 Real-Time PCR system using the Universal SYBR Green Fast qPCR mix (Bio-Rad Laboratories) as described before [57].

    Concentration Assay:

    Article Title: Senescent Fibroblasts Impair B Cell Proliferation via Cell–Cell Contact
    Article Snippet: RNA from 0.5‐1 × 10 7 cells was purified using the RNeasy Mini Kit (Qiagen, cat. no. 74104) following the manufacturer's instructions. .. RNA concentration was quantified using a NanoDrop One (Thermo Scientific) and 1 μg RNA converted into cDNA using the iScript cDNA Synthesis Kit (Bio Rad, #1708891) and stored at −20°C until use. .. Taqman primer‐probes were used for qPCR: CDKN2A (Hs00923894_m1), CDKN1A (Hs00355782_m1) and IL6 (Hs00174131_m1) were assayed alongside VAMP7 (Hs00194568_m1) and L3MBT2L (Hs01002034_m1) as housekeeper genes.

    Reverse Transcription:

    Article Title: Andrographolide Promotes Post-Ischemic Endothelial Angiogenic Activity via IL-10-Mediated M2-Like Microglia-Endothelial Crosstalk Involving Endothelial VEGFR2 Upregulation.
    Article Snippet: The RNA was quantified using a spectrophotometer (Denovix (Wilmington, DE, USA)). .. RNA (1000 ng) was used as a template and was subjected to reverse transcription using an iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). .. Real-time quantitative PCR (RT-qPCR) was performed in duplicate for each sample using a Bio-Rad Real-Time PCR system (CFX96 Optics Module BioRad).

    Article Title: Radiofrequency Radiation Alters Redox Homeostasis, ATP Status, and Apoptosis-Associated Responses in Human Ovarian Epithelial Cells.
    Article Snippet: RNA quantity and quality were assessed via NanoDrop spectrophotometry. .. cDNA synthesis from 500 ng total RNA utilized the Bio-Rad iScript cDNA Synthesis Kit in a 10 μL reaction volume comprising 2 μL 5X iScript reaction mix, 0.5 μL iScript reverse transcriptase, RNA template, and nuclease-free water. ..

    Real-time Polymerase Chain Reaction:

    Article Title: The homologous GRAS transcription factors RAM1 and RAD1 coordinate shared and separate gene networks to regulate arbuscular mycorrhiza symbiosis.
    Article Snippet: Genomic DNA was removed using DNase I (AMPD1, Sigma-Aldrich) or TURBODNA-freeTM Kit (Ambion). .. cDNAwas synthesized using the iScriptTM cDNA Synthesis Kit (Bio-Rad), and qPCR was performed with primers shown in Table S1 on a Bio-Rad CFX 384 qPCR system or a Roche LIGHTCYCLER 480 system using SybrMaster (Jena Bioscience, Jena, Germany). (a) (b) (c) (d) (e) (f) 2026 The Author(s). ..



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