bsai restriction endonuclease (New England Biolabs)
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bsai restriction endonuclease
Bsai Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+program+for+dna+sequencing/BsaI-HF+v2/pmc07901297-139-0-4
Average 97 stars, based on 2168 article reviews
Bsai Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+program+for+dna+sequencing/BsaI-HF+v2/pmc07901297-139-0-4
Average 97 stars, based on 2168 article reviews
bsai restriction endonuclease - by Bioz Stars,
2026-10
97/100 stars
Images
1) Product Images from "A trans -complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence"
Article Title: A trans -complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence
Journal: Cell
doi: 10.1016/j.cell.2021.02.044
Figure Legend Snippet:
Techniques Used: Recombinant, SYBR Green Assay, Electroporation, Gel Extraction, Electron Microscopy, Synthesized, Sequencing, Software
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Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK-293T ATCC CRL-3216 HeLa ATCC CCL-2 K562 ATCC CCL-243 N2a ATCC CCL-131 10 Molecular Systems Biology © The Author(s) Reagent/resource Reference or source Identifier or catalog number C2C12 ATCC CRL-1772 Mouse (C57BL/6), male, 4 weeks old Jiangsu GemPharmatech Co., Ltd SCXK (Su) 2018- 0008 Recombinant DNA AncBE4max Addgene #112094 ABE-max Addgene #112095 ABE-8e Addgene #138495 pCMV-nCas-PmCDA1 Addgene #79620 pCMV-AsCas12a Addgene #160140 pCMV-SpCas9 Addgene #139987 pGL3-U6-sgRNA-mCherry Our lab pCMV-CgCas12n Our lab pCMV-AcCas12n Our lab pCMV-enUnCas12f Our lab pCMV-TSminiCBE Our lab Antibodies Anti-Myhc DSHB MF 20 Anti-Dystrophin DSHB MANDRA1(7A10) Anti-Dystrophin Proteintech 12715-1-AP Anti-gapdh Proteintech 10494-1-AP Donkey anti-Mouse IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen A-21202 Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen A-31572 HRP Goat Anti-Rabbit IgG (H+ L) Abclonal AS014 HRP Goat Anti-Mouse IgG (H+ L) Abclonal AS003 Oligonucleotides and other sequence-based reagents Target sequence This study Appendix Tables S4–S8 Primer used for qPCR This study Appendix Table S9 Chemicals, enzymes, and other reagents Real-time Polymerase Chain Reaction:Article Title: Engineering a compact CgCas12n platform for efficient genome and base editing in mammalian cells. 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Article Snippet: .. 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The molecular reagents used included but were not limited to NheI-HF (NEB [ Ipswich, MA, USA], R3131), BamH1-HF (NEB, R3136), DNA Ligation:Article Title: Long-Term Labeling and Differentiation Monitoring of Mouse Spermatogonial Stem Cells Using CellREADR Article Snippet: .. The molecular reagents used included but were not limited to NheI-HF (NEB [ Ipswich, MA, USA], R3131), BamH1-HF (NEB, R3136), |