imagequant tl 8 1 p 00110 (Danaher Inc)
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imagequant tl 8 1 p 00110
Imagequant Tl 8 1 P 00110, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+package/IQTL+8%2E1+and+IQTLSecurITy+8%2E1+Software+Package/pm30017914-77-101-96
Average 95 stars, based on 39 article reviews
Imagequant Tl 8 1 P 00110, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+package/IQTL+8%2E1+and+IQTLSecurITy+8%2E1+Software+Package/pm30017914-77-101-96
Average 95 stars, based on 39 article reviews
imagequant tl 8 1 p 00110 - by Bioz Stars,
2026-11
95/100 stars
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Purification:Article Title: Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation Article Snippet: .. Purified hMIGA2 long was mixed with 1 μl of NBD-labeled lipids (at 1 mg/ml) in 20 μl total reaction volumes and incubated on ice for 2 h. Samples were loaded onto 4–15% Mini-Protean Precast Native gels and run for 90 min at 100 V. NBD fluorescence was visualized using an Incubation:Article Title: Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation Article Snippet: .. Purified hMIGA2 long was mixed with 1 μl of NBD-labeled lipids (at 1 mg/ml) in 20 μl total reaction volumes and incubated on ice for 2 h. Samples were loaded onto 4–15% Mini-Protean Precast Native gels and run for 90 min at 100 V. NBD fluorescence was visualized using an Fluorescence:Article Title: Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation Article Snippet: .. Purified hMIGA2 long was mixed with 1 μl of NBD-labeled lipids (at 1 mg/ml) in 20 μl total reaction volumes and incubated on ice for 2 h. Samples were loaded onto 4–15% Mini-Protean Precast Native gels and run for 90 min at 100 V. NBD fluorescence was visualized using an Software:Article Title: Demethyleugenol β-Glucopyranoside Isolated from Agastache rugosa Decreases Melanin Synthesis via Down-regulation of MITF and SOX9. Article Snippet: Journal of Agricultural and Food Chemistry is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society. Article Title: Sequencing Mirror-Image DNA Chemically. Article Snippet: .. MG1655 Tiandz Cat#12-211 Chemicals, Peptides, and Recombinant Proteins Hydroxylamine hydrochloride Sigma-Aldrich Cat#159417 Glycogen Thermo Fisher Scientific Cat#R0561 Triethylamine Sinopharm Chemical Reagent Cat#80134318 Hydrazine hydrate Sinopharm Chemical Reagent Cat#80070418 Formic acid Amresco Cat#VWRV0916 Methylene blue Amresco Cat#97062-276 Sodium hydroxide Sigma-Aldrich Cat#S5881 Ethnol Sinopharm Chemical Reagent Cat#10009159 Yeast tRNA Solarbio Cat#T8630-100 L-deoxynucleoside phosphoramidites ChemGenes N/A Formamide Amresco Cat#97061-392 Urea Sigma-Aldrich Cat#U5378 Critical Commercial Assays PAGE DNA Purification Kit Tiandz Cat#70908-30 Oligonucleotides D-/L-FAM-primer12 50-FAM-ACTACGAACGCG-30 This paper N/A L-FAM-primer11 50-FAM-CGCGCTGTTAT-30 This paper N/A L-FAM-primer25 50-FAM-ATGCCTGGCAGTTCCCTACTCTCGC-30 This paper N/A L-FAM-primer55 50-FAM-TCACGTGCATGATAGACGGCGAAGCC GTCGAGTTGCTGTGTGCCGATGCACGTGA-30 This paper N/A Software and Algorithms ImageQuant TL 8.1 GE Healthcare Life Sciences https://www.gelifesciences.com/en/us/shop/proteinanalysis/molecular-imaging-for-proteins/imaging-software/ Article Title: Telomere Loop Dynamics in Chromosome End Protection. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-53BP1 Santa Cruz Cat# Sc-22760 Mouse Anti-b-Actin Sigma Cat# A2228 Rabbit Anti-ATM Cell Signaling Technology Cat# 2873 Rabbit Anti-ATM-ser1981 Abcam Cat# ab81292 Mouse Anti-CHK2 Millipore Cat# 05-649 Rabbit Anti-Phospho-Histone H3-ser10 Alexa Fluor 488 conjugate Cell Signaling Technology Cat# 9708 Mouse Anti-g-H2AX Millipore Cat# 05-636 Mouse Anti-Myc-Tag Cell Signaling Technology Cat# 2276 Rabbit Anti-TRF2 Novus Biologicals Cat# NB110-57130 Mouse Anti-Vinculin Sigma Cat# V9131 Goat anti-Mouse, Alexa Fluor 568 Thermo Fisher Scientific Cat# A11031 Goat anti-Mouse, Alexa Fluor 488 Thermo Fisher Scientific Cat # A28175 Goat anti-Rabbit, Alexa Fluor 647 Thermo Fisher Scientific Cat# A27040 Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat# P0448 Polyclonal Goat Anti-Mouse Immunoglobulins/HRP Dako Cat# P0447 Bacterial and Virus Strains E. coli HST08 Clontech Cat# 636766 Chemicals, Peptides, and Recombinant Proteins 4-hydroxytamoxifen Sigma-Aldrich Cat# H7904 Benzonase Merck Millipore Cat# 70746 Hesperadin Selleckchem Cat# S1529 HinfI New England Biolabs Cat# R0155S Ku-55933 Merck Millipore Cat# 118500 Lovastatin Sigma Cat# PHR1285 DL-Mevalonic acid lactone Sigma Cat# M4667 Micrococcal nuclease New England Biolabs Cat# M0247S Protease inhibitor cocktail Roche Cat# 05892791001 RsaI New England Biolabs Cat# R0167S TelC-Alexa fluor 488 (Alexa488-OO-ccctaaccctaaccctaa) Panagene Cat# F1004 TelC-Alexa fluor 647 (Alexa674-OO-ccctaaccctaaccctaa) Panagene Cat# F2003 Trioxsalen Sigma-Aldrich Cat# T6137 Cysteamine Sigma-Aldrich Cat# M9768 YoYo-1 Thermo Fisher Scientific Cat# Y3601 ProLong Diamond Antifade Mountant Thermo Fisher Scientific Cat# P36965 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36930 Critical Commercial Assays SuperSignal West Femto Thermo Fisher Scientific Cat# 34095 SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 (Continued on next page) Molecular Cell 71, 1–16.e1–e6, August 16, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines TRF2Floxed/Floxed Rosa26-CreERT2 pBabeSV40LT murine embryonic fibroblasts Eros Lazzerini-Denchi (The Scripps Research Institute) Okamoto et al., 2013 293T Tracy Bryan (CMRI) N/A HT1080 6TG Eric Stanbridge (University of California at Irvine) N/A HeLa LT Jan Karlseder (Salk Institute) O’Sullivan et al., 2014 IMR90 E6E7 Jan Karlseder (Salk Institute) Cesare et al., 2013 U-2OS Jan Karlseder (Salk Institute) N/A Oligonucleotides (CCCTAA)3 Integrated DNA Technologies N/A Recombinant DNA pVPack-GP Vector Agilent Cat# 217566 pVPack-Ampho Vector Agilent Cat# 217568 pLPC-Myc-TRF2 Addgene Cat# 16066 pLPC-Myc-TRFcT Addgene Cat# 44573 pLKO.1 non-targeting control Addgene Cat# 1864 pLKO.1 TRF2 sh-F The Broad Institute Cat# TRCN0000004811 pLKO.1 TRF2 sh-G The Broad Institute Cat# TRCN0000018358 Software and Algorithms ZEISS Zen Black and Zen Blue ZEISS https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html Metafer 4 and ISIS Metasystems https://metasystems-international.com/ ImageJ Version 1.51h NIH, USA https://imagej.nih.gov/ij SoftWorX Version 6.5.2 GE Healthcare Life Sciences http://incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/6.5.2/SoftWoRx.htm IMARIS 3D/4D Version 9.1.2 Bitplane http://www.bitplane.com/imaris/imaris OMX Master Control Software - AcquireSR softWoRx reconstruction and analysis software GE Healthcare Life Sciences http://www.gelifesciences.com/en/au/shop/ cell-imaging-and-analysis/high-and-super- resolution-microscopes Leica Las X Leica Microsystems https://www.leica-microsystems.com/products/ microscope-software/details/product/leica-las-x-ls/ Huygens Professional Edition Scientific Volume Imaging https://svi.nl/Huygens-Professional FACS Diva Version 8 BD Biosciences https://www.bdbiosciences.com/in/instruments/ software/facsdiva/features/overview.jsp FlowJo Version 7.2.2 FlowJo https://www.flowjo.com/solutions/flowjo/downloads/ other:Article Title: Structural Basis for Target-Directed MicroRNA Degradation Article Snippet: ImageQuant TL , GE Healthcare Life Sciences , Cat# 290006 05. Article Title: H 2 pyhox - Octadentate Bis(pyridyloxine). Article Snippet: A new versatile chelating ligand for intermediate size and softness radiometals [Cu]Cu and [In]In, H2pyhox, was synthesized by introducing pyridine as a new donor moiety to complement 8-hydroxyquinoline on an ethylenediamine backbone.. The combination of pyridine and oxine as donor sets was explored through structural analysis, and crystals of the three metal complexes with Cu, La, and In demonstrate how the ligand adapts to accommodate metal ions of different sizes and charge.. Exhaustive in-batch UV solution studies characterized the protonation constants of the free ligand as well as the formation constants of the metal complexes with Cu, In, and La. Recombinant:Article Title: Sequencing Mirror-Image DNA Chemically. Article Snippet: .. MG1655 Tiandz Cat#12-211 Chemicals, Peptides, and Recombinant Proteins Hydroxylamine hydrochloride Sigma-Aldrich Cat#159417 Glycogen Thermo Fisher Scientific Cat#R0561 Triethylamine Sinopharm Chemical Reagent Cat#80134318 Hydrazine hydrate Sinopharm Chemical Reagent Cat#80070418 Formic acid Amresco Cat#VWRV0916 Methylene blue Amresco Cat#97062-276 Sodium hydroxide Sigma-Aldrich Cat#S5881 Ethnol Sinopharm Chemical Reagent Cat#10009159 Yeast tRNA Solarbio Cat#T8630-100 L-deoxynucleoside phosphoramidites ChemGenes N/A Formamide Amresco Cat#97061-392 Urea Sigma-Aldrich Cat#U5378 Critical Commercial Assays PAGE DNA Purification Kit Tiandz Cat#70908-30 Oligonucleotides D-/L-FAM-primer12 50-FAM-ACTACGAACGCG-30 This paper N/A L-FAM-primer11 50-FAM-CGCGCTGTTAT-30 This paper N/A L-FAM-primer25 50-FAM-ATGCCTGGCAGTTCCCTACTCTCGC-30 This paper N/A L-FAM-primer55 50-FAM-TCACGTGCATGATAGACGGCGAAGCC GTCGAGTTGCTGTGTGCCGATGCACGTGA-30 This paper N/A Software and Algorithms ImageQuant TL 8.1 GE Healthcare Life Sciences https://www.gelifesciences.com/en/us/shop/proteinanalysis/molecular-imaging-for-proteins/imaging-software/ Article Title: Telomere Loop Dynamics in Chromosome End Protection. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-53BP1 Santa Cruz Cat# Sc-22760 Mouse Anti-b-Actin Sigma Cat# A2228 Rabbit Anti-ATM Cell Signaling Technology Cat# 2873 Rabbit Anti-ATM-ser1981 Abcam Cat# ab81292 Mouse Anti-CHK2 Millipore Cat# 05-649 Rabbit Anti-Phospho-Histone H3-ser10 Alexa Fluor 488 conjugate Cell Signaling Technology Cat# 9708 Mouse Anti-g-H2AX Millipore Cat# 05-636 Mouse Anti-Myc-Tag Cell Signaling Technology Cat# 2276 Rabbit Anti-TRF2 Novus Biologicals Cat# NB110-57130 Mouse Anti-Vinculin Sigma Cat# V9131 Goat anti-Mouse, Alexa Fluor 568 Thermo Fisher Scientific Cat# A11031 Goat anti-Mouse, Alexa Fluor 488 Thermo Fisher Scientific Cat # A28175 Goat anti-Rabbit, Alexa Fluor 647 Thermo Fisher Scientific Cat# A27040 Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat# P0448 Polyclonal Goat Anti-Mouse Immunoglobulins/HRP Dako Cat# P0447 Bacterial and Virus Strains E. coli HST08 Clontech Cat# 636766 Chemicals, Peptides, and Recombinant Proteins 4-hydroxytamoxifen Sigma-Aldrich Cat# H7904 Benzonase Merck Millipore Cat# 70746 Hesperadin Selleckchem Cat# S1529 HinfI New England Biolabs Cat# R0155S Ku-55933 Merck Millipore Cat# 118500 Lovastatin Sigma Cat# PHR1285 DL-Mevalonic acid lactone Sigma Cat# M4667 Micrococcal nuclease New England Biolabs Cat# M0247S Protease inhibitor cocktail Roche Cat# 05892791001 RsaI New England Biolabs Cat# R0167S TelC-Alexa fluor 488 (Alexa488-OO-ccctaaccctaaccctaa) Panagene Cat# F1004 TelC-Alexa fluor 647 (Alexa674-OO-ccctaaccctaaccctaa) Panagene Cat# F2003 Trioxsalen Sigma-Aldrich Cat# T6137 Cysteamine Sigma-Aldrich Cat# M9768 YoYo-1 Thermo Fisher Scientific Cat# Y3601 ProLong Diamond Antifade Mountant Thermo Fisher Scientific Cat# P36965 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36930 Critical Commercial Assays SuperSignal West Femto Thermo Fisher Scientific Cat# 34095 SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 (Continued on next page) Molecular Cell 71, 1–16.e1–e6, August 16, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines TRF2Floxed/Floxed Rosa26-CreERT2 pBabeSV40LT murine embryonic fibroblasts Eros Lazzerini-Denchi (The Scripps Research Institute) Okamoto et al., 2013 293T Tracy Bryan (CMRI) N/A HT1080 6TG Eric Stanbridge (University of California at Irvine) N/A HeLa LT Jan Karlseder (Salk Institute) O’Sullivan et al., 2014 IMR90 E6E7 Jan Karlseder (Salk Institute) Cesare et al., 2013 U-2OS Jan Karlseder (Salk Institute) N/A Oligonucleotides (CCCTAA)3 Integrated DNA Technologies N/A Recombinant DNA pVPack-GP Vector Agilent Cat# 217566 pVPack-Ampho Vector Agilent Cat# 217568 pLPC-Myc-TRF2 Addgene Cat# 16066 pLPC-Myc-TRFcT Addgene Cat# 44573 pLKO.1 non-targeting control Addgene Cat# 1864 pLKO.1 TRF2 sh-F The Broad Institute Cat# TRCN0000004811 pLKO.1 TRF2 sh-G The Broad Institute Cat# TRCN0000018358 Software and Algorithms ZEISS Zen Black and Zen Blue ZEISS https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html Metafer 4 and ISIS Metasystems https://metasystems-international.com/ ImageJ Version 1.51h NIH, USA https://imagej.nih.gov/ij SoftWorX Version 6.5.2 GE Healthcare Life Sciences http://incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/6.5.2/SoftWoRx.htm IMARIS 3D/4D Version 9.1.2 Bitplane http://www.bitplane.com/imaris/imaris OMX Master Control Software - AcquireSR softWoRx reconstruction and analysis software GE Healthcare Life Sciences http://www.gelifesciences.com/en/au/shop/ cell-imaging-and-analysis/high-and-super- resolution-microscopes Leica Las X Leica Microsystems https://www.leica-microsystems.com/products/ microscope-software/details/product/leica-las-x-ls/ Huygens Professional Edition Scientific Volume Imaging https://svi.nl/Huygens-Professional FACS Diva Version 8 BD Biosciences https://www.bdbiosciences.com/in/instruments/ software/facsdiva/features/overview.jsp FlowJo Version 7.2.2 FlowJo https://www.flowjo.com/solutions/flowjo/downloads/ Polyacrylamide Gel Electrophoresis:Article Title: Sequencing Mirror-Image DNA Chemically. Article Snippet: .. MG1655 Tiandz Cat#12-211 Chemicals, Peptides, and Recombinant Proteins Hydroxylamine hydrochloride Sigma-Aldrich Cat#159417 Glycogen Thermo Fisher Scientific Cat#R0561 Triethylamine Sinopharm Chemical Reagent Cat#80134318 Hydrazine hydrate Sinopharm Chemical Reagent Cat#80070418 Formic acid Amresco Cat#VWRV0916 Methylene blue Amresco Cat#97062-276 Sodium hydroxide Sigma-Aldrich Cat#S5881 Ethnol Sinopharm Chemical Reagent Cat#10009159 Yeast tRNA Solarbio Cat#T8630-100 L-deoxynucleoside phosphoramidites ChemGenes N/A Formamide Amresco Cat#97061-392 Urea Sigma-Aldrich Cat#U5378 Critical Commercial Assays PAGE DNA Purification Kit Tiandz Cat#70908-30 Oligonucleotides D-/L-FAM-primer12 50-FAM-ACTACGAACGCG-30 This paper N/A L-FAM-primer11 50-FAM-CGCGCTGTTAT-30 This paper N/A L-FAM-primer25 50-FAM-ATGCCTGGCAGTTCCCTACTCTCGC-30 This paper N/A L-FAM-primer55 50-FAM-TCACGTGCATGATAGACGGCGAAGCC GTCGAGTTGCTGTGTGCCGATGCACGTGA-30 This paper N/A Software and Algorithms ImageQuant TL 8.1 GE Healthcare Life Sciences https://www.gelifesciences.com/en/us/shop/proteinanalysis/molecular-imaging-for-proteins/imaging-software/ DNA Purification:Article Title: Sequencing Mirror-Image DNA Chemically. Article Snippet: .. MG1655 Tiandz Cat#12-211 Chemicals, Peptides, and Recombinant Proteins Hydroxylamine hydrochloride Sigma-Aldrich Cat#159417 Glycogen Thermo Fisher Scientific Cat#R0561 Triethylamine Sinopharm Chemical Reagent Cat#80134318 Hydrazine hydrate Sinopharm Chemical Reagent Cat#80070418 Formic acid Amresco Cat#VWRV0916 Methylene blue Amresco Cat#97062-276 Sodium hydroxide Sigma-Aldrich Cat#S5881 Ethnol Sinopharm Chemical Reagent Cat#10009159 Yeast tRNA Solarbio Cat#T8630-100 L-deoxynucleoside phosphoramidites ChemGenes N/A Formamide Amresco Cat#97061-392 Urea Sigma-Aldrich Cat#U5378 Critical Commercial Assays PAGE DNA Purification Kit Tiandz Cat#70908-30 Oligonucleotides D-/L-FAM-primer12 50-FAM-ACTACGAACGCG-30 This paper N/A L-FAM-primer11 50-FAM-CGCGCTGTTAT-30 This paper N/A L-FAM-primer25 50-FAM-ATGCCTGGCAGTTCCCTACTCTCGC-30 This paper N/A L-FAM-primer55 50-FAM-TCACGTGCATGATAGACGGCGAAGCC GTCGAGTTGCTGTGTGCCGATGCACGTGA-30 This paper N/A Software and Algorithms ImageQuant TL 8.1 GE Healthcare Life Sciences https://www.gelifesciences.com/en/us/shop/proteinanalysis/molecular-imaging-for-proteins/imaging-software/ Control:Article Title: Telomere Loop Dynamics in Chromosome End Protection. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-53BP1 Santa Cruz Cat# Sc-22760 Mouse Anti-b-Actin Sigma Cat# A2228 Rabbit Anti-ATM Cell Signaling Technology Cat# 2873 Rabbit Anti-ATM-ser1981 Abcam Cat# ab81292 Mouse Anti-CHK2 Millipore Cat# 05-649 Rabbit Anti-Phospho-Histone H3-ser10 Alexa Fluor 488 conjugate Cell Signaling Technology Cat# 9708 Mouse Anti-g-H2AX Millipore Cat# 05-636 Mouse Anti-Myc-Tag Cell Signaling Technology Cat# 2276 Rabbit Anti-TRF2 Novus Biologicals Cat# NB110-57130 Mouse Anti-Vinculin Sigma Cat# V9131 Goat anti-Mouse, Alexa Fluor 568 Thermo Fisher Scientific Cat# A11031 Goat anti-Mouse, Alexa Fluor 488 Thermo Fisher Scientific Cat # A28175 Goat anti-Rabbit, Alexa Fluor 647 Thermo Fisher Scientific Cat# A27040 Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat# P0448 Polyclonal Goat Anti-Mouse Immunoglobulins/HRP Dako Cat# P0447 Bacterial and Virus Strains E. coli HST08 Clontech Cat# 636766 Chemicals, Peptides, and Recombinant Proteins 4-hydroxytamoxifen Sigma-Aldrich Cat# H7904 Benzonase Merck Millipore Cat# 70746 Hesperadin Selleckchem Cat# S1529 HinfI New England Biolabs Cat# R0155S Ku-55933 Merck Millipore Cat# 118500 Lovastatin Sigma Cat# PHR1285 DL-Mevalonic acid lactone Sigma Cat# M4667 Micrococcal nuclease New England Biolabs Cat# M0247S Protease inhibitor cocktail Roche Cat# 05892791001 RsaI New England Biolabs Cat# R0167S TelC-Alexa fluor 488 (Alexa488-OO-ccctaaccctaaccctaa) Panagene Cat# F1004 TelC-Alexa fluor 647 (Alexa674-OO-ccctaaccctaaccctaa) Panagene Cat# F2003 Trioxsalen Sigma-Aldrich Cat# T6137 Cysteamine Sigma-Aldrich Cat# M9768 YoYo-1 Thermo Fisher Scientific Cat# Y3601 ProLong Diamond Antifade Mountant Thermo Fisher Scientific Cat# P36965 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36930 Critical Commercial Assays SuperSignal West Femto Thermo Fisher Scientific Cat# 34095 SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 (Continued on next page) Molecular Cell 71, 1–16.e1–e6, August 16, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines TRF2Floxed/Floxed Rosa26-CreERT2 pBabeSV40LT murine embryonic fibroblasts Eros Lazzerini-Denchi (The Scripps Research Institute) Okamoto et al., 2013 293T Tracy Bryan (CMRI) N/A HT1080 6TG Eric Stanbridge (University of California at Irvine) N/A HeLa LT Jan Karlseder (Salk Institute) O’Sullivan et al., 2014 IMR90 E6E7 Jan Karlseder (Salk Institute) Cesare et al., 2013 U-2OS Jan Karlseder (Salk Institute) N/A Oligonucleotides (CCCTAA)3 Integrated DNA Technologies N/A Recombinant DNA pVPack-GP Vector Agilent Cat# 217566 pVPack-Ampho Vector Agilent Cat# 217568 pLPC-Myc-TRF2 Addgene Cat# 16066 pLPC-Myc-TRFcT Addgene Cat# 44573 pLKO.1 non-targeting control Addgene Cat# 1864 pLKO.1 TRF2 sh-F The Broad Institute Cat# TRCN0000004811 pLKO.1 TRF2 sh-G The Broad Institute Cat# TRCN0000018358 Software and Algorithms ZEISS Zen Black and Zen Blue ZEISS https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html Metafer 4 and ISIS Metasystems https://metasystems-international.com/ ImageJ Version 1.51h NIH, USA https://imagej.nih.gov/ij SoftWorX Version 6.5.2 GE Healthcare Life Sciences http://incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/6.5.2/SoftWoRx.htm IMARIS 3D/4D Version 9.1.2 Bitplane http://www.bitplane.com/imaris/imaris OMX Master Control Software - AcquireSR softWoRx reconstruction and analysis software GE Healthcare Life Sciences http://www.gelifesciences.com/en/au/shop/ cell-imaging-and-analysis/high-and-super- resolution-microscopes Leica Las X Leica Microsystems https://www.leica-microsystems.com/products/ microscope-software/details/product/leica-las-x-ls/ Huygens Professional Edition Scientific Volume Imaging https://svi.nl/Huygens-Professional FACS Diva Version 8 BD Biosciences https://www.bdbiosciences.com/in/instruments/ software/facsdiva/features/overview.jsp FlowJo Version 7.2.2 FlowJo https://www.flowjo.com/solutions/flowjo/downloads/ Microscopy:Article Title: Telomere Loop Dynamics in Chromosome End Protection. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-53BP1 Santa Cruz Cat# Sc-22760 Mouse Anti-b-Actin Sigma Cat# A2228 Rabbit Anti-ATM Cell Signaling Technology Cat# 2873 Rabbit Anti-ATM-ser1981 Abcam Cat# ab81292 Mouse Anti-CHK2 Millipore Cat# 05-649 Rabbit Anti-Phospho-Histone H3-ser10 Alexa Fluor 488 conjugate Cell Signaling Technology Cat# 9708 Mouse Anti-g-H2AX Millipore Cat# 05-636 Mouse Anti-Myc-Tag Cell Signaling Technology Cat# 2276 Rabbit Anti-TRF2 Novus Biologicals Cat# NB110-57130 Mouse Anti-Vinculin Sigma Cat# V9131 Goat anti-Mouse, Alexa Fluor 568 Thermo Fisher Scientific Cat# A11031 Goat anti-Mouse, Alexa Fluor 488 Thermo Fisher Scientific Cat # A28175 Goat anti-Rabbit, Alexa Fluor 647 Thermo Fisher Scientific Cat# A27040 Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat# P0448 Polyclonal Goat Anti-Mouse Immunoglobulins/HRP Dako Cat# P0447 Bacterial and Virus Strains E. coli HST08 Clontech Cat# 636766 Chemicals, Peptides, and Recombinant Proteins 4-hydroxytamoxifen Sigma-Aldrich Cat# H7904 Benzonase Merck Millipore Cat# 70746 Hesperadin Selleckchem Cat# S1529 HinfI New England Biolabs Cat# R0155S Ku-55933 Merck Millipore Cat# 118500 Lovastatin Sigma Cat# PHR1285 DL-Mevalonic acid lactone Sigma Cat# M4667 Micrococcal nuclease New England Biolabs Cat# M0247S Protease inhibitor cocktail Roche Cat# 05892791001 RsaI New England Biolabs Cat# R0167S TelC-Alexa fluor 488 (Alexa488-OO-ccctaaccctaaccctaa) Panagene Cat# F1004 TelC-Alexa fluor 647 (Alexa674-OO-ccctaaccctaaccctaa) Panagene Cat# F2003 Trioxsalen Sigma-Aldrich Cat# T6137 Cysteamine Sigma-Aldrich Cat# M9768 YoYo-1 Thermo Fisher Scientific Cat# Y3601 ProLong Diamond Antifade Mountant Thermo Fisher Scientific Cat# P36965 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36930 Critical Commercial Assays SuperSignal West Femto Thermo Fisher Scientific Cat# 34095 SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 (Continued on next page) Molecular Cell 71, 1–16.e1–e6, August 16, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines TRF2Floxed/Floxed Rosa26-CreERT2 pBabeSV40LT murine embryonic fibroblasts Eros Lazzerini-Denchi (The Scripps Research Institute) Okamoto et al., 2013 293T Tracy Bryan (CMRI) N/A HT1080 6TG Eric Stanbridge (University of California at Irvine) N/A HeLa LT Jan Karlseder (Salk Institute) O’Sullivan et al., 2014 IMR90 E6E7 Jan Karlseder (Salk Institute) Cesare et al., 2013 U-2OS Jan Karlseder (Salk Institute) N/A Oligonucleotides (CCCTAA)3 Integrated DNA Technologies N/A Recombinant DNA pVPack-GP Vector Agilent Cat# 217566 pVPack-Ampho Vector Agilent Cat# 217568 pLPC-Myc-TRF2 Addgene Cat# 16066 pLPC-Myc-TRFcT Addgene Cat# 44573 pLKO.1 non-targeting control Addgene Cat# 1864 pLKO.1 TRF2 sh-F The Broad Institute Cat# TRCN0000004811 pLKO.1 TRF2 sh-G The Broad Institute Cat# TRCN0000018358 Software and Algorithms ZEISS Zen Black and Zen Blue ZEISS https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html Metafer 4 and ISIS Metasystems https://metasystems-international.com/ ImageJ Version 1.51h NIH, USA https://imagej.nih.gov/ij SoftWorX Version 6.5.2 GE Healthcare Life Sciences http://incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/6.5.2/SoftWoRx.htm IMARIS 3D/4D Version 9.1.2 Bitplane http://www.bitplane.com/imaris/imaris OMX Master Control Software - AcquireSR softWoRx reconstruction and analysis software GE Healthcare Life Sciences http://www.gelifesciences.com/en/au/shop/ cell-imaging-and-analysis/high-and-super- resolution-microscopes Leica Las X Leica Microsystems https://www.leica-microsystems.com/products/ microscope-software/details/product/leica-las-x-ls/ Huygens Professional Edition Scientific Volume Imaging https://svi.nl/Huygens-Professional FACS Diva Version 8 BD Biosciences https://www.bdbiosciences.com/in/instruments/ software/facsdiva/features/overview.jsp FlowJo Version 7.2.2 FlowJo https://www.flowjo.com/solutions/flowjo/downloads/ Imaging:Article Title: Telomere Loop Dynamics in Chromosome End Protection. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-53BP1 Santa Cruz Cat# Sc-22760 Mouse Anti-b-Actin Sigma Cat# A2228 Rabbit Anti-ATM Cell Signaling Technology Cat# 2873 Rabbit Anti-ATM-ser1981 Abcam Cat# ab81292 Mouse Anti-CHK2 Millipore Cat# 05-649 Rabbit Anti-Phospho-Histone H3-ser10 Alexa Fluor 488 conjugate Cell Signaling Technology Cat# 9708 Mouse Anti-g-H2AX Millipore Cat# 05-636 Mouse Anti-Myc-Tag Cell Signaling Technology Cat# 2276 Rabbit Anti-TRF2 Novus Biologicals Cat# NB110-57130 Mouse Anti-Vinculin Sigma Cat# V9131 Goat anti-Mouse, Alexa Fluor 568 Thermo Fisher Scientific Cat# A11031 Goat anti-Mouse, Alexa Fluor 488 Thermo Fisher Scientific Cat # A28175 Goat anti-Rabbit, Alexa Fluor 647 Thermo Fisher Scientific Cat# A27040 Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat# P0448 Polyclonal Goat Anti-Mouse Immunoglobulins/HRP Dako Cat# P0447 Bacterial and Virus Strains E. coli HST08 Clontech Cat# 636766 Chemicals, Peptides, and Recombinant Proteins 4-hydroxytamoxifen Sigma-Aldrich Cat# H7904 Benzonase Merck Millipore Cat# 70746 Hesperadin Selleckchem Cat# S1529 HinfI New England Biolabs Cat# R0155S Ku-55933 Merck Millipore Cat# 118500 Lovastatin Sigma Cat# PHR1285 DL-Mevalonic acid lactone Sigma Cat# M4667 Micrococcal nuclease New England Biolabs Cat# M0247S Protease inhibitor cocktail Roche Cat# 05892791001 RsaI New England Biolabs Cat# R0167S TelC-Alexa fluor 488 (Alexa488-OO-ccctaaccctaaccctaa) Panagene Cat# F1004 TelC-Alexa fluor 647 (Alexa674-OO-ccctaaccctaaccctaa) Panagene Cat# F2003 Trioxsalen Sigma-Aldrich Cat# T6137 Cysteamine Sigma-Aldrich Cat# M9768 YoYo-1 Thermo Fisher Scientific Cat# Y3601 ProLong Diamond Antifade Mountant Thermo Fisher Scientific Cat# P36965 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36930 Critical Commercial Assays SuperSignal West Femto Thermo Fisher Scientific Cat# 34095 SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 (Continued on next page) Molecular Cell 71, 1–16.e1–e6, August 16, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines TRF2Floxed/Floxed Rosa26-CreERT2 pBabeSV40LT murine embryonic fibroblasts Eros Lazzerini-Denchi (The Scripps Research Institute) Okamoto et al., 2013 293T Tracy Bryan (CMRI) N/A HT1080 6TG Eric Stanbridge (University of California at Irvine) N/A HeLa LT Jan Karlseder (Salk Institute) O’Sullivan et al., 2014 IMR90 E6E7 Jan Karlseder (Salk Institute) Cesare et al., 2013 U-2OS Jan Karlseder (Salk Institute) N/A Oligonucleotides (CCCTAA)3 Integrated DNA Technologies N/A Recombinant DNA pVPack-GP Vector Agilent Cat# 217566 pVPack-Ampho Vector Agilent Cat# 217568 pLPC-Myc-TRF2 Addgene Cat# 16066 pLPC-Myc-TRFcT Addgene Cat# 44573 pLKO.1 non-targeting control Addgene Cat# 1864 pLKO.1 TRF2 sh-F The Broad Institute Cat# TRCN0000004811 pLKO.1 TRF2 sh-G The Broad Institute Cat# TRCN0000018358 Software and Algorithms ZEISS Zen Black and Zen Blue ZEISS https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html Metafer 4 and ISIS Metasystems https://metasystems-international.com/ ImageJ Version 1.51h NIH, USA https://imagej.nih.gov/ij SoftWorX Version 6.5.2 GE Healthcare Life Sciences http://incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/6.5.2/SoftWoRx.htm IMARIS 3D/4D Version 9.1.2 Bitplane http://www.bitplane.com/imaris/imaris OMX Master Control Software - AcquireSR softWoRx reconstruction and analysis software GE Healthcare Life Sciences http://www.gelifesciences.com/en/au/shop/ cell-imaging-and-analysis/high-and-super- resolution-microscopes Leica Las X Leica Microsystems https://www.leica-microsystems.com/products/ microscope-software/details/product/leica-las-x-ls/ Huygens Professional Edition Scientific Volume Imaging https://svi.nl/Huygens-Professional FACS Diva Version 8 BD Biosciences https://www.bdbiosciences.com/in/instruments/ software/facsdiva/features/overview.jsp FlowJo Version 7.2.2 FlowJo https://www.flowjo.com/solutions/flowjo/downloads/ FACS:Article Title: Telomere Loop Dynamics in Chromosome End Protection. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-53BP1 Santa Cruz Cat# Sc-22760 Mouse Anti-b-Actin Sigma Cat# A2228 Rabbit Anti-ATM Cell Signaling Technology Cat# 2873 Rabbit Anti-ATM-ser1981 Abcam Cat# ab81292 Mouse Anti-CHK2 Millipore Cat# 05-649 Rabbit Anti-Phospho-Histone H3-ser10 Alexa Fluor 488 conjugate Cell Signaling Technology Cat# 9708 Mouse Anti-g-H2AX Millipore Cat# 05-636 Mouse Anti-Myc-Tag Cell Signaling Technology Cat# 2276 Rabbit Anti-TRF2 Novus Biologicals Cat# NB110-57130 Mouse Anti-Vinculin Sigma Cat# V9131 Goat anti-Mouse, Alexa Fluor 568 Thermo Fisher Scientific Cat# A11031 Goat anti-Mouse, Alexa Fluor 488 Thermo Fisher Scientific Cat # A28175 Goat anti-Rabbit, Alexa Fluor 647 Thermo Fisher Scientific Cat# A27040 Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP Dako Cat# P0448 Polyclonal Goat Anti-Mouse Immunoglobulins/HRP Dako Cat# P0447 Bacterial and Virus Strains E. coli HST08 Clontech Cat# 636766 Chemicals, Peptides, and Recombinant Proteins 4-hydroxytamoxifen Sigma-Aldrich Cat# H7904 Benzonase Merck Millipore Cat# 70746 Hesperadin Selleckchem Cat# S1529 HinfI New England Biolabs Cat# R0155S Ku-55933 Merck Millipore Cat# 118500 Lovastatin Sigma Cat# PHR1285 DL-Mevalonic acid lactone Sigma Cat# M4667 Micrococcal nuclease New England Biolabs Cat# M0247S Protease inhibitor cocktail Roche Cat# 05892791001 RsaI New England Biolabs Cat# R0167S TelC-Alexa fluor 488 (Alexa488-OO-ccctaaccctaaccctaa) Panagene Cat# F1004 TelC-Alexa fluor 647 (Alexa674-OO-ccctaaccctaaccctaa) Panagene Cat# F2003 Trioxsalen Sigma-Aldrich Cat# T6137 Cysteamine Sigma-Aldrich Cat# M9768 YoYo-1 Thermo Fisher Scientific Cat# Y3601 ProLong Diamond Antifade Mountant Thermo Fisher Scientific Cat# P36965 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36930 Critical Commercial Assays SuperSignal West Femto Thermo Fisher Scientific Cat# 34095 SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 (Continued on next page) Molecular Cell 71, 1–16.e1–e6, August 16, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines TRF2Floxed/Floxed Rosa26-CreERT2 pBabeSV40LT murine embryonic fibroblasts Eros Lazzerini-Denchi (The Scripps Research Institute) Okamoto et al., 2013 293T Tracy Bryan (CMRI) N/A HT1080 6TG Eric Stanbridge (University of California at Irvine) N/A HeLa LT Jan Karlseder (Salk Institute) O’Sullivan et al., 2014 IMR90 E6E7 Jan Karlseder (Salk Institute) Cesare et al., 2013 U-2OS Jan Karlseder (Salk Institute) N/A Oligonucleotides (CCCTAA)3 Integrated DNA Technologies N/A Recombinant DNA pVPack-GP Vector Agilent Cat# 217566 pVPack-Ampho Vector Agilent Cat# 217568 pLPC-Myc-TRF2 Addgene Cat# 16066 pLPC-Myc-TRFcT Addgene Cat# 44573 pLKO.1 non-targeting control Addgene Cat# 1864 pLKO.1 TRF2 sh-F The Broad Institute Cat# TRCN0000004811 pLKO.1 TRF2 sh-G The Broad Institute Cat# TRCN0000018358 Software and Algorithms ZEISS Zen Black and Zen Blue ZEISS https://www.zeiss.com/microscopy/int/products/ microscope-software/zen.html Metafer 4 and ISIS Metasystems https://metasystems-international.com/ ImageJ Version 1.51h NIH, USA https://imagej.nih.gov/ij SoftWorX Version 6.5.2 GE Healthcare Life Sciences http://incelldownload.gehealthcare.com/bin/ download_data/SoftWoRx/6.5.2/SoftWoRx.htm IMARIS 3D/4D Version 9.1.2 Bitplane http://www.bitplane.com/imaris/imaris OMX Master Control Software - AcquireSR softWoRx reconstruction and analysis software GE Healthcare Life Sciences http://www.gelifesciences.com/en/au/shop/ cell-imaging-and-analysis/high-and-super- resolution-microscopes Leica Las X Leica Microsystems https://www.leica-microsystems.com/products/ microscope-software/details/product/leica-las-x-ls/ Huygens Professional Edition Scientific Volume Imaging https://svi.nl/Huygens-Professional FACS Diva Version 8 BD Biosciences https://www.bdbiosciences.com/in/instruments/ software/facsdiva/features/overview.jsp FlowJo Version 7.2.2 FlowJo https://www.flowjo.com/solutions/flowjo/downloads/ |