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Journal: Molecular Metabolism

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

doi: 10.1016/j.molmet.2025.102175

Figure Lengend Snippet: Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

Techniques: Western Blot, Immunofluorescence, Staining

Journal: Molecular Metabolism

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

doi: 10.1016/j.molmet.2025.102175

Figure Lengend Snippet: Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

Techniques: RNA Sequencing, Immunofluorescence, Staining, Control, Mutagenesis, Expressing