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MetaMorph Inc image analysis software
Effect of radiation therapy/resveratrol (XRT/RSV) on the expression of pro‐ and antiproliferative molecules evaluated by immunohistochemistry and Western blot. (a) Immunohistochemistry for p21, p27, and p53 in PC‐3 cells treated with or without XRT (8 Gy) in the presence or absence of RSV (50 μM). Original magnification, ×400. (b) Relative immunostaining intensity for p21, p27, and p53 in five to six randomly selected high power fields of three slides from each group analyzed by <t>MetaMorph</t> <t>software.</t> Results are expressed as the average integrated immunostaining intensity of three slides + SEM relative to that in control cells. (c) Protein expression levels of p21, p27, and p53 by Western blot <t>analysis;</t> 30 μg protein was loaded in each lane. *P < 0.05, significant difference in immunostaining intensity or protein expression level in the groups treated with XRT or XRT/RSV versus controls. #P < 0.05, significant difference in immunostaining intensity or protein expression level between the group treated with XRT/RSV versus the group treated with XRT alone. Figures shown are representative of two or three independent experiments.
Image Analysis Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
image analysis software - by Bioz Stars, 2026-11
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Images

1) Product Images from "Resveratrol enhances radiation sensitivity in prostate cancer by inhibiting cell proliferation and promoting cell senescence and apoptosis"

Article Title: Resveratrol enhances radiation sensitivity in prostate cancer by inhibiting cell proliferation and promoting cell senescence and apoptosis

Journal: Cancer Science

doi: 10.1111/j.1349-7006.2012.02272.x

Effect of radiation therapy/resveratrol (XRT/RSV) on the expression of pro‐ and antiproliferative molecules evaluated by immunohistochemistry and Western blot. (a) Immunohistochemistry for p21, p27, and p53 in PC‐3 cells treated with or without XRT (8 Gy) in the presence or absence of RSV (50 μM). Original magnification, ×400. (b) Relative immunostaining intensity for p21, p27, and p53 in five to six randomly selected high power fields of three slides from each group analyzed by MetaMorph software. Results are expressed as the average integrated immunostaining intensity of three slides + SEM relative to that in control cells. (c) Protein expression levels of p21, p27, and p53 by Western blot analysis; 30 μg protein was loaded in each lane. *P < 0.05, significant difference in immunostaining intensity or protein expression level in the groups treated with XRT or XRT/RSV versus controls. #P < 0.05, significant difference in immunostaining intensity or protein expression level between the group treated with XRT/RSV versus the group treated with XRT alone. Figures shown are representative of two or three independent experiments.
Figure Legend Snippet: Effect of radiation therapy/resveratrol (XRT/RSV) on the expression of pro‐ and antiproliferative molecules evaluated by immunohistochemistry and Western blot. (a) Immunohistochemistry for p21, p27, and p53 in PC‐3 cells treated with or without XRT (8 Gy) in the presence or absence of RSV (50 μM). Original magnification, ×400. (b) Relative immunostaining intensity for p21, p27, and p53 in five to six randomly selected high power fields of three slides from each group analyzed by MetaMorph software. Results are expressed as the average integrated immunostaining intensity of three slides + SEM relative to that in control cells. (c) Protein expression levels of p21, p27, and p53 by Western blot analysis; 30 μg protein was loaded in each lane. *P < 0.05, significant difference in immunostaining intensity or protein expression level in the groups treated with XRT or XRT/RSV versus controls. #P < 0.05, significant difference in immunostaining intensity or protein expression level between the group treated with XRT/RSV versus the group treated with XRT alone. Figures shown are representative of two or three independent experiments.

Techniques Used: Expressing, Immunohistochemistry, Western Blot, Immunostaining, Software, Control

Effect of radiation therapy/resveratrol (XRT/RSV) on the expression of p‐AKT and p‐H2A.X evaluated by immunohistochemistry and Western blot analysis. (a) Immunohistochemistry for p‐AKT and p‐H2A.X in PC‐3 cells treated with or without XRT (8 Gy) in the presence or absence of RSV (50 μM). Original magnification, ×400. (b) Relative immunostaining intensity for p‐AKT and p‐H2A.X was analyzed by MetaMorph software. Results are expressed as the average integrated immunostaining intensity of three slides + SEM relative to that in control cells. (c) Shown are Western blot results for the protein expression levels of p‐AKT and p‐H2A.X; 30 μg protein was loaded in each lane. *P < 0.05, significant difference in immunostaining intensity or protein expression level in the groups treated with XRT or XRT/RSV versus controls. #P < 0.05, significant difference in immunostaining intensity or protein expression level between the group treated with XRT/RSV versus the group treated with XRT alone. Figures are representative of two or three independent experiments.
Figure Legend Snippet: Effect of radiation therapy/resveratrol (XRT/RSV) on the expression of p‐AKT and p‐H2A.X evaluated by immunohistochemistry and Western blot analysis. (a) Immunohistochemistry for p‐AKT and p‐H2A.X in PC‐3 cells treated with or without XRT (8 Gy) in the presence or absence of RSV (50 μM). Original magnification, ×400. (b) Relative immunostaining intensity for p‐AKT and p‐H2A.X was analyzed by MetaMorph software. Results are expressed as the average integrated immunostaining intensity of three slides + SEM relative to that in control cells. (c) Shown are Western blot results for the protein expression levels of p‐AKT and p‐H2A.X; 30 μg protein was loaded in each lane. *P < 0.05, significant difference in immunostaining intensity or protein expression level in the groups treated with XRT or XRT/RSV versus controls. #P < 0.05, significant difference in immunostaining intensity or protein expression level between the group treated with XRT/RSV versus the group treated with XRT alone. Figures are representative of two or three independent experiments.

Techniques Used: Expressing, Immunohistochemistry, Western Blot, Immunostaining, Software, Control

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Article Title: Methods of using 4(1
Article Snippet: In Vitro Microtubule Nucleation Assay Purified centrosomes were incubated with or without AS1712 and with porcine tubulin (Cytoskeleton) in 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, and 1 mM GTP, pH 6.8 for 8 min at 37° C. Microtubule asters were fixed in 1% glutaraldehyde, sedimented onto acid-treated coverslips, and finally subjected to immunofluorescence with anti-α-tubulin. .. The fiber lengths of the microtubule asters were measured using MetaMorph image analysis software (n=20/group). .. In Vitro Tubulin Polymerization Assay Reagents from a Tubulin Polymerization Assay kit (Cytoskeleton) were used according to the manufacturer's instructions.

Article Title: Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles
Article Snippet: .. Fluorescent images of the cells or tissue sections were captured using a deconvolution microscope (Olympus Korea Co. Ltd., Seoul, Korea) and processed with image analysis software such as ImageJ or MetaMorph software (MetaMorph Inc., San Jose, CA, USA). .. Cells or tissues obtained from mice were lysed in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor cocktail and phenylmethylsulfonyl fluoride (#52332; Sigma-Aldrich).

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Article Title: Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles
Article Snippet: .. Fluorescent images of the cells or tissue sections were captured using a deconvolution microscope (Olympus Korea Co. Ltd., Seoul, Korea) and processed with image analysis software such as ImageJ or MetaMorph software (MetaMorph Inc., San Jose, CA, USA). .. Cells or tissues obtained from mice were lysed in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor cocktail and phenylmethylsulfonyl fluoride (#52332; Sigma-Aldrich).

Microscopy:

Article Title: Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles
Article Snippet: .. Fluorescent images of the cells or tissue sections were captured using a deconvolution microscope (Olympus Korea Co. Ltd., Seoul, Korea) and processed with image analysis software such as ImageJ or MetaMorph software (MetaMorph Inc., San Jose, CA, USA). .. Cells or tissues obtained from mice were lysed in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor cocktail and phenylmethylsulfonyl fluoride (#52332; Sigma-Aldrich).

Analysis:

Article Title: Activation of Immune Responses Through the RIG-I Pathway Using TRITC-Dextran Encapsulated Nanoparticles
Article Snippet: .. Fluorescent images of the cells or tissue sections were captured using a deconvolution microscope (Olympus Korea Co. Ltd., Seoul, Korea) and processed with image analysis software such as ImageJ or MetaMorph software (MetaMorph Inc., San Jose, CA, USA). .. Cells or tissues obtained from mice were lysed in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor cocktail and phenylmethylsulfonyl fluoride (#52332; Sigma-Aldrich).

Immunofluorescence:

Article Title: In Vivo Differentiation of Endogenous Bone Marrow-Derived Cells into Insulin-Producing Cells Using Four Soluble Factors
Article Snippet: .. These observations were determined by quantification of immunofluorescence images using the Metamorph Image Analysis tool, which revealed a significant increase in insulin-positive and GFP-positive cell areas in the group administered with diff. cocktail. ..



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Image Search Results


Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Journal: Molecular Metabolism

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

doi: 10.1016/j.molmet.2025.102175

Figure Lengend Snippet: Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

Techniques: Western Blot, Immunofluorescence, Staining

Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Journal: Molecular Metabolism

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

doi: 10.1016/j.molmet.2025.102175

Figure Lengend Snippet: Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

Techniques: RNA Sequencing, Immunofluorescence, Staining, Control, Mutagenesis, Expressing