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Deepmind Technologies Ltd algorithms graphpad prism 10 4 1 graphpad software
Algorithms Graphpad Prism 10 4 1 Graphpad Software, supplied by Deepmind Technologies Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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algorithms graphpad prism 10 4 1 graphpad software - by Bioz Stars, 2026-10
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Article Title: Polyglutamine homorepeat regulates Runx2 condensation and cellular localization in a KPNA3-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER C2C12 Cell Bank of the Chinese Academy of Sciences CSTR:19375.09.3101MOUSCSP505 MC3T3-E1 Subclone 14 Cell Bank of the Chinese Academy of Sciences CSTR:19375.09.3101MOUGNM15 Oligonucleotides A list of primers used in this experiment is listed in Table S1 This paper N/A A list of primers used in this experiment is listed in Table S2 This paper N/A A list of siRNAs used in this experiment is listed in Table S3 This paper N/A A list of primary sequences obtained from UniProt in this experiment is listed in Table S4 This paper N/A Software and algorithms GraphPad Prism 10.4.1 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Zeiss ZEN Microscopy Software 3.8 lite Zeiss https://www.zeiss.com/microscopy/en/products/software/ zeiss-zen.html AlphaFold3 DeepMind https://alphafoldserver.com PONDR Molecular Kinetics https://www.pondr.com/ UniProt NIH https://www.uniprot.org/ Pymol 3.0 Schrödinger https://pymol.org/ ChimeraX NIH https://www.rbvi.ucsf.edu/chimerax Jalview ELIXIR-UK https://www.jalview.org/ Clustal Omega EMBL-EBI http://www.clustal.org/omega/ Cell Reports 45, 117205, April 28, 2026 17 .. As previously describe,60 total RNA was isolated using an AFTSpin Tissue/Cell Fast RNA Extraction Kit (Abclonal, RK30120).

Article Title: Nociceptive neurons inhibit neutrophil extracellular trap formation via MLKL-licensed histone release.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER HRP-conjugated goat anti-rat IgG(H + L) Thermo Fisher Scientific Cat#A18865;RRID:AB_2535642 HRP-conjugated mouse anti-rabbit IgG light chain Abclonal Cat#AS061;RRID:AB_2864055 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-Mlkl(K219A, K241A,K244A,K245A)-3xFLAG-WPRE OBIO HYKY-221031067-DAAV pAAV-hSyn-EGFP-P2A-Mlkl(196-472aa)3xFLAG-WPRE OBIO HYKY-240710013-DAAV pAAV-hSyn-EGFP-P2A-Mlkl-3xFLAGWPRE OBIO HYKY-220704019-DAAV pAAV-U6-shRNA(P2rx7)-CMV-EGFPWPRE OBIO HYKY-240711037-DAAV pCDNA3.1(+)-Hist1h3a-HA OBIO H49744 pCDNA3.1(+)-Histlh3a(E134A)-HA OBIO H49745 pcDNA3.1(+)-MIkI-3xFLAG OBIO H49746 DCDNA3.1(+)-MIkI(R105A)-3XFLAG OBIO H49747 Chemicals, peptides, and recombinant proteins DAPI Sigma Aldrich Cat#D9542 Leptomycin B Aladdin Cat#L102387 DNase1 Sigma Aldrich Cat#10104159001 Recombinant Histone H3.1 Active Motif Cat#31894 Sytox Green Invitrogen Cat#S7020 Critical commercial assays Histone H3 Total Elisa Kit Abcam Cat#ab115091 DeadENDTM TUNEL system Promega Cat#G3250 PinpoRNATM2.0 Pinpoease Cat#PIF1000 Deposited data Raw data of proteomics and IP-MS This paper; ProteomeXchange Consortium, iProX partner repository24,25 Dataset identifier: PXD077273 Experimental models: Cell lines 293T BeNa Culture Collection BNCC353535 Experimental models: Organisms/strains Mlkl− /− mouse Gift from Prof. Jiahuai Han N/A Ripk3− /− mouse Gift from Dr. Dixit VM N/A Nav1.8-Cre mouse Gift from Prof.Rohini Kuner N/A Mlklfl/fl mouse Cyagen 220617BY1 Tlr4− /− mouse Shanghai model organisms N/A Software and algorithms Prism 8.0 GraphPad https://www.graphpad.com/ SPSS 21.0 IBM https://www.statistical-analysis.top/ ImageJ NRIUS https://imagej.net/ij/ AlphaFold3.0 DeepMind/Google https://alphafoldserver.com/ HDOCK N/A http://hdock.phys.hust.edu.cn/ PyMOL3.03 Schrödinger https://pymol.org/ 12 Cell Reports 45, 117457, June 23, 2026 University and conducted in accordance with ARRIVE guidelines. ..

Microscopy:

Article Title: Polyglutamine homorepeat regulates Runx2 condensation and cellular localization in a KPNA3-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER C2C12 Cell Bank of the Chinese Academy of Sciences CSTR:19375.09.3101MOUSCSP505 MC3T3-E1 Subclone 14 Cell Bank of the Chinese Academy of Sciences CSTR:19375.09.3101MOUGNM15 Oligonucleotides A list of primers used in this experiment is listed in Table S1 This paper N/A A list of primers used in this experiment is listed in Table S2 This paper N/A A list of siRNAs used in this experiment is listed in Table S3 This paper N/A A list of primary sequences obtained from UniProt in this experiment is listed in Table S4 This paper N/A Software and algorithms GraphPad Prism 10.4.1 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Zeiss ZEN Microscopy Software 3.8 lite Zeiss https://www.zeiss.com/microscopy/en/products/software/ zeiss-zen.html AlphaFold3 DeepMind https://alphafoldserver.com PONDR Molecular Kinetics https://www.pondr.com/ UniProt NIH https://www.uniprot.org/ Pymol 3.0 Schrödinger https://pymol.org/ ChimeraX NIH https://www.rbvi.ucsf.edu/chimerax Jalview ELIXIR-UK https://www.jalview.org/ Clustal Omega EMBL-EBI http://www.clustal.org/omega/ Cell Reports 45, 117205, April 28, 2026 17 .. As previously describe,60 total RNA was isolated using an AFTSpin Tissue/Cell Fast RNA Extraction Kit (Abclonal, RK30120).

Virus:

Article Title: Nociceptive neurons inhibit neutrophil extracellular trap formation via MLKL-licensed histone release.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER HRP-conjugated goat anti-rat IgG(H + L) Thermo Fisher Scientific Cat#A18865;RRID:AB_2535642 HRP-conjugated mouse anti-rabbit IgG light chain Abclonal Cat#AS061;RRID:AB_2864055 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-Mlkl(K219A, K241A,K244A,K245A)-3xFLAG-WPRE OBIO HYKY-221031067-DAAV pAAV-hSyn-EGFP-P2A-Mlkl(196-472aa)3xFLAG-WPRE OBIO HYKY-240710013-DAAV pAAV-hSyn-EGFP-P2A-Mlkl-3xFLAGWPRE OBIO HYKY-220704019-DAAV pAAV-U6-shRNA(P2rx7)-CMV-EGFPWPRE OBIO HYKY-240711037-DAAV pCDNA3.1(+)-Hist1h3a-HA OBIO H49744 pCDNA3.1(+)-Histlh3a(E134A)-HA OBIO H49745 pcDNA3.1(+)-MIkI-3xFLAG OBIO H49746 DCDNA3.1(+)-MIkI(R105A)-3XFLAG OBIO H49747 Chemicals, peptides, and recombinant proteins DAPI Sigma Aldrich Cat#D9542 Leptomycin B Aladdin Cat#L102387 DNase1 Sigma Aldrich Cat#10104159001 Recombinant Histone H3.1 Active Motif Cat#31894 Sytox Green Invitrogen Cat#S7020 Critical commercial assays Histone H3 Total Elisa Kit Abcam Cat#ab115091 DeadENDTM TUNEL system Promega Cat#G3250 PinpoRNATM2.0 Pinpoease Cat#PIF1000 Deposited data Raw data of proteomics and IP-MS This paper; ProteomeXchange Consortium, iProX partner repository24,25 Dataset identifier: PXD077273 Experimental models: Cell lines 293T BeNa Culture Collection BNCC353535 Experimental models: Organisms/strains Mlkl− /− mouse Gift from Prof. Jiahuai Han N/A Ripk3− /− mouse Gift from Dr. Dixit VM N/A Nav1.8-Cre mouse Gift from Prof.Rohini Kuner N/A Mlklfl/fl mouse Cyagen 220617BY1 Tlr4− /− mouse Shanghai model organisms N/A Software and algorithms Prism 8.0 GraphPad https://www.graphpad.com/ SPSS 21.0 IBM https://www.statistical-analysis.top/ ImageJ NRIUS https://imagej.net/ij/ AlphaFold3.0 DeepMind/Google https://alphafoldserver.com/ HDOCK N/A http://hdock.phys.hust.edu.cn/ PyMOL3.03 Schrödinger https://pymol.org/ 12 Cell Reports 45, 117457, June 23, 2026 University and conducted in accordance with ARRIVE guidelines. ..

Recombinant:

Article Title: Nociceptive neurons inhibit neutrophil extracellular trap formation via MLKL-licensed histone release.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER HRP-conjugated goat anti-rat IgG(H + L) Thermo Fisher Scientific Cat#A18865;RRID:AB_2535642 HRP-conjugated mouse anti-rabbit IgG light chain Abclonal Cat#AS061;RRID:AB_2864055 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-Mlkl(K219A, K241A,K244A,K245A)-3xFLAG-WPRE OBIO HYKY-221031067-DAAV pAAV-hSyn-EGFP-P2A-Mlkl(196-472aa)3xFLAG-WPRE OBIO HYKY-240710013-DAAV pAAV-hSyn-EGFP-P2A-Mlkl-3xFLAGWPRE OBIO HYKY-220704019-DAAV pAAV-U6-shRNA(P2rx7)-CMV-EGFPWPRE OBIO HYKY-240711037-DAAV pCDNA3.1(+)-Hist1h3a-HA OBIO H49744 pCDNA3.1(+)-Histlh3a(E134A)-HA OBIO H49745 pcDNA3.1(+)-MIkI-3xFLAG OBIO H49746 DCDNA3.1(+)-MIkI(R105A)-3XFLAG OBIO H49747 Chemicals, peptides, and recombinant proteins DAPI Sigma Aldrich Cat#D9542 Leptomycin B Aladdin Cat#L102387 DNase1 Sigma Aldrich Cat#10104159001 Recombinant Histone H3.1 Active Motif Cat#31894 Sytox Green Invitrogen Cat#S7020 Critical commercial assays Histone H3 Total Elisa Kit Abcam Cat#ab115091 DeadENDTM TUNEL system Promega Cat#G3250 PinpoRNATM2.0 Pinpoease Cat#PIF1000 Deposited data Raw data of proteomics and IP-MS This paper; ProteomeXchange Consortium, iProX partner repository24,25 Dataset identifier: PXD077273 Experimental models: Cell lines 293T BeNa Culture Collection BNCC353535 Experimental models: Organisms/strains Mlkl− /− mouse Gift from Prof. Jiahuai Han N/A Ripk3− /− mouse Gift from Dr. Dixit VM N/A Nav1.8-Cre mouse Gift from Prof.Rohini Kuner N/A Mlklfl/fl mouse Cyagen 220617BY1 Tlr4− /− mouse Shanghai model organisms N/A Software and algorithms Prism 8.0 GraphPad https://www.graphpad.com/ SPSS 21.0 IBM https://www.statistical-analysis.top/ ImageJ NRIUS https://imagej.net/ij/ AlphaFold3.0 DeepMind/Google https://alphafoldserver.com/ HDOCK N/A http://hdock.phys.hust.edu.cn/ PyMOL3.03 Schrödinger https://pymol.org/ 12 Cell Reports 45, 117457, June 23, 2026 University and conducted in accordance with ARRIVE guidelines. ..

Enzyme-linked Immunosorbent Assay:

Article Title: Nociceptive neurons inhibit neutrophil extracellular trap formation via MLKL-licensed histone release.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER HRP-conjugated goat anti-rat IgG(H + L) Thermo Fisher Scientific Cat#A18865;RRID:AB_2535642 HRP-conjugated mouse anti-rabbit IgG light chain Abclonal Cat#AS061;RRID:AB_2864055 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-Mlkl(K219A, K241A,K244A,K245A)-3xFLAG-WPRE OBIO HYKY-221031067-DAAV pAAV-hSyn-EGFP-P2A-Mlkl(196-472aa)3xFLAG-WPRE OBIO HYKY-240710013-DAAV pAAV-hSyn-EGFP-P2A-Mlkl-3xFLAGWPRE OBIO HYKY-220704019-DAAV pAAV-U6-shRNA(P2rx7)-CMV-EGFPWPRE OBIO HYKY-240711037-DAAV pCDNA3.1(+)-Hist1h3a-HA OBIO H49744 pCDNA3.1(+)-Histlh3a(E134A)-HA OBIO H49745 pcDNA3.1(+)-MIkI-3xFLAG OBIO H49746 DCDNA3.1(+)-MIkI(R105A)-3XFLAG OBIO H49747 Chemicals, peptides, and recombinant proteins DAPI Sigma Aldrich Cat#D9542 Leptomycin B Aladdin Cat#L102387 DNase1 Sigma Aldrich Cat#10104159001 Recombinant Histone H3.1 Active Motif Cat#31894 Sytox Green Invitrogen Cat#S7020 Critical commercial assays Histone H3 Total Elisa Kit Abcam Cat#ab115091 DeadENDTM TUNEL system Promega Cat#G3250 PinpoRNATM2.0 Pinpoease Cat#PIF1000 Deposited data Raw data of proteomics and IP-MS This paper; ProteomeXchange Consortium, iProX partner repository24,25 Dataset identifier: PXD077273 Experimental models: Cell lines 293T BeNa Culture Collection BNCC353535 Experimental models: Organisms/strains Mlkl− /− mouse Gift from Prof. Jiahuai Han N/A Ripk3− /− mouse Gift from Dr. Dixit VM N/A Nav1.8-Cre mouse Gift from Prof.Rohini Kuner N/A Mlklfl/fl mouse Cyagen 220617BY1 Tlr4− /− mouse Shanghai model organisms N/A Software and algorithms Prism 8.0 GraphPad https://www.graphpad.com/ SPSS 21.0 IBM https://www.statistical-analysis.top/ ImageJ NRIUS https://imagej.net/ij/ AlphaFold3.0 DeepMind/Google https://alphafoldserver.com/ HDOCK N/A http://hdock.phys.hust.edu.cn/ PyMOL3.03 Schrödinger https://pymol.org/ 12 Cell Reports 45, 117457, June 23, 2026 University and conducted in accordance with ARRIVE guidelines. ..

TUNEL Assay:

Article Title: Nociceptive neurons inhibit neutrophil extracellular trap formation via MLKL-licensed histone release.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER HRP-conjugated goat anti-rat IgG(H + L) Thermo Fisher Scientific Cat#A18865;RRID:AB_2535642 HRP-conjugated mouse anti-rabbit IgG light chain Abclonal Cat#AS061;RRID:AB_2864055 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-Mlkl(K219A, K241A,K244A,K245A)-3xFLAG-WPRE OBIO HYKY-221031067-DAAV pAAV-hSyn-EGFP-P2A-Mlkl(196-472aa)3xFLAG-WPRE OBIO HYKY-240710013-DAAV pAAV-hSyn-EGFP-P2A-Mlkl-3xFLAGWPRE OBIO HYKY-220704019-DAAV pAAV-U6-shRNA(P2rx7)-CMV-EGFPWPRE OBIO HYKY-240711037-DAAV pCDNA3.1(+)-Hist1h3a-HA OBIO H49744 pCDNA3.1(+)-Histlh3a(E134A)-HA OBIO H49745 pcDNA3.1(+)-MIkI-3xFLAG OBIO H49746 DCDNA3.1(+)-MIkI(R105A)-3XFLAG OBIO H49747 Chemicals, peptides, and recombinant proteins DAPI Sigma Aldrich Cat#D9542 Leptomycin B Aladdin Cat#L102387 DNase1 Sigma Aldrich Cat#10104159001 Recombinant Histone H3.1 Active Motif Cat#31894 Sytox Green Invitrogen Cat#S7020 Critical commercial assays Histone H3 Total Elisa Kit Abcam Cat#ab115091 DeadENDTM TUNEL system Promega Cat#G3250 PinpoRNATM2.0 Pinpoease Cat#PIF1000 Deposited data Raw data of proteomics and IP-MS This paper; ProteomeXchange Consortium, iProX partner repository24,25 Dataset identifier: PXD077273 Experimental models: Cell lines 293T BeNa Culture Collection BNCC353535 Experimental models: Organisms/strains Mlkl− /− mouse Gift from Prof. Jiahuai Han N/A Ripk3− /− mouse Gift from Dr. Dixit VM N/A Nav1.8-Cre mouse Gift from Prof.Rohini Kuner N/A Mlklfl/fl mouse Cyagen 220617BY1 Tlr4− /− mouse Shanghai model organisms N/A Software and algorithms Prism 8.0 GraphPad https://www.graphpad.com/ SPSS 21.0 IBM https://www.statistical-analysis.top/ ImageJ NRIUS https://imagej.net/ij/ AlphaFold3.0 DeepMind/Google https://alphafoldserver.com/ HDOCK N/A http://hdock.phys.hust.edu.cn/ PyMOL3.03 Schrödinger https://pymol.org/ 12 Cell Reports 45, 117457, June 23, 2026 University and conducted in accordance with ARRIVE guidelines. ..

Protein-Protein interactions:

Article Title: Nociceptive neurons inhibit neutrophil extracellular trap formation via MLKL-licensed histone release.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER HRP-conjugated goat anti-rat IgG(H + L) Thermo Fisher Scientific Cat#A18865;RRID:AB_2535642 HRP-conjugated mouse anti-rabbit IgG light chain Abclonal Cat#AS061;RRID:AB_2864055 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-Mlkl(K219A, K241A,K244A,K245A)-3xFLAG-WPRE OBIO HYKY-221031067-DAAV pAAV-hSyn-EGFP-P2A-Mlkl(196-472aa)3xFLAG-WPRE OBIO HYKY-240710013-DAAV pAAV-hSyn-EGFP-P2A-Mlkl-3xFLAGWPRE OBIO HYKY-220704019-DAAV pAAV-U6-shRNA(P2rx7)-CMV-EGFPWPRE OBIO HYKY-240711037-DAAV pCDNA3.1(+)-Hist1h3a-HA OBIO H49744 pCDNA3.1(+)-Histlh3a(E134A)-HA OBIO H49745 pcDNA3.1(+)-MIkI-3xFLAG OBIO H49746 DCDNA3.1(+)-MIkI(R105A)-3XFLAG OBIO H49747 Chemicals, peptides, and recombinant proteins DAPI Sigma Aldrich Cat#D9542 Leptomycin B Aladdin Cat#L102387 DNase1 Sigma Aldrich Cat#10104159001 Recombinant Histone H3.1 Active Motif Cat#31894 Sytox Green Invitrogen Cat#S7020 Critical commercial assays Histone H3 Total Elisa Kit Abcam Cat#ab115091 DeadENDTM TUNEL system Promega Cat#G3250 PinpoRNATM2.0 Pinpoease Cat#PIF1000 Deposited data Raw data of proteomics and IP-MS This paper; ProteomeXchange Consortium, iProX partner repository24,25 Dataset identifier: PXD077273 Experimental models: Cell lines 293T BeNa Culture Collection BNCC353535 Experimental models: Organisms/strains Mlkl− /− mouse Gift from Prof. Jiahuai Han N/A Ripk3− /− mouse Gift from Dr. Dixit VM N/A Nav1.8-Cre mouse Gift from Prof.Rohini Kuner N/A Mlklfl/fl mouse Cyagen 220617BY1 Tlr4− /− mouse Shanghai model organisms N/A Software and algorithms Prism 8.0 GraphPad https://www.graphpad.com/ SPSS 21.0 IBM https://www.statistical-analysis.top/ ImageJ NRIUS https://imagej.net/ij/ AlphaFold3.0 DeepMind/Google https://alphafoldserver.com/ HDOCK N/A http://hdock.phys.hust.edu.cn/ PyMOL3.03 Schrödinger https://pymol.org/ 12 Cell Reports 45, 117457, June 23, 2026 University and conducted in accordance with ARRIVE guidelines. ..

other:

Article Title: De novo recovery of Ghana virus, an African bat Henipavirus, reveals differential tropism and attenuated pathogenicity compared to Nipah virus.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Direct-zol TM RNA Miniprep Kit Zymo Research Cat# R2052 Direct RNA Sequencing Kit Oxford Nanopore Technologies Cat# SQK-RNA004 Deposited data Whole-genome sequencing of rGhV M-GS * stocks This study NCBI BioProject: PRJNA1284405 Direct RNA sequencing (Nanopore) of rGhV M-GS * infected CHO-B2 cells This study NCBI BioProject: PRJNA1307648 Numerical data underlying figures This study Mendeley Data: https://doi.org/10.17632/ rjgcxt4cxr.1 Experimental models: cell lines BSR-T7/5 Karl-Klaus Conzelmann RRID:CVCL_RW96 Vero CCL-81 ATCC Cat# CCL-81; RRID:CVCL_0059 HEK-293T ATCC Cat# CRL-3216; RRID:CVCL_0063 BKT1 Maeda et al., 2008 RRID:CVCL_YZ67 FBKT1 Maeda et al., 2008 RRID: CVCL_YZ69 ZFBK11-97 Maruyama et al., 2014 RRID: CVCL_YZ74 ZFBS13-75A Maruyama et al., 2016 RRID: CVCL_YZ77 EidNi/41.2 Susanne et al., 2011 RRID:CVCL_RX13 ZFBK13-75 van Tol et al., 2023 N/A CHO-B2 Negrete et al., 2006 N/A CHO-B3 Negrete et al., 2006 N/A Experimental models: organisms/strains Syrian golden hamster (Mesocricetus auratus) Envigo N/A Oligonucleotides EFNB2 Primer for RT-qPCR (Forward) MilliporeSigma 5 ′ -AGGGACTCCGTGTGGAAGTA-3 ′ EFNB2 Primer for RT-qPCR (Reverse) MilliporeSigma 5 ′ -AGAGTCCACTTTGGGGCAAAT-3 ′ EFNB3 Primer for RT-qPCR (Forward) MilliporeSigma 5 ′ -TCTCCGCTTCACCATCAAGT-3 ′ EFNB3 Primer for RT-qPCR (Reverse) MilliporeSigma 5 ′ -TCGGAGAAGCACCTTCATGC-3 ′ GAPDH Primer for RT-qPCR (Forward) MilliporeSigma 5 ′ -TCAAGGGCATCCTGGGCTA-3 ′ GAPDH Primer for RT-qPCR (Reverse) MilliporeSigma 5 ′ -ACCACCCTGTTGCTGTAGCCAA-3 ′ Recombinant DNA Recombinant Ghana virus reverse genetics plasmids (N, P, L, and antigenome constructs) This study GenBank: PX138233-PX138236 Codon-optimized T7 RNA polyermase Yun et al., 2015 Addgene #65974 Software and algorithms GraphPad Prism 10.3.1 GraphPad Software https://www.graphpad.com/ UCSF ChimeraX UCSF https://www.cgl.ucsf.edu/chimerax/ ImageJ ImageJ https://imagej.net/ij/ MEGA X MEGA https://www.megasoftware.net/ AlphaFold3 Server Google DeepMind and Isomorphic Labs https://alphafoldserver.com/ MinKNOW 24.11.8 Oxford Nanopore Technologies https://nanoporetech.com/document/ experiment-companion-minknow Dorado (basecalling) Oxford Nanopore Technologies Release 7.6.7 Minimap2 Li et al., 2018 https://github.com/lh3/minimap2 SAMtools Danecek er al., 2021 https://www.htslib.org/ FlowJo v10.9.0 BD Biosciences https://www.flowjo.com/ (Continued on next page) Cell Reports 45, 117074, March 24, 2026 17



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