algorithms prism v10 graphpad (Jackson Laboratory)
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Jackson Laboratory
algorithms prism v10 graphpad
Algorithms Prism V10 Graphpad, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+graphpad/0+0+9+algorithms+devices+graphpad+graphpad+i3x+molecular+prism+reader+software+spectramax+version/pm40019835-285-46-39
Average 86 stars, based on 1 article reviews
Algorithms Prism V10 Graphpad, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/software+graphpad/0+0+9+algorithms+devices+graphpad+graphpad+i3x+molecular+prism+reader+software+spectramax+version/pm40019835-285-46-39
Average 86 stars, based on 1 article reviews
algorithms prism v10 graphpad - by Bioz Stars,
2026-10
86/100 stars
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Generated:Article Title: Neural stem cell relay from B1 to B2 cells in the adult mouse ventricular-subventricular zone. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mm-Zeb1-C1 ADCBio Cat#451201 Brdu Sigma-Aldrich Cat# B5002 Tamoxifen Sigma-Aldrich Cat# T5648 Violet Cell Trace Thermo Fisher Scientific Cat#C34557 40,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) Sigma-Aldrich Cat# D9542 3H-thymidine Perkin Elmer Cat# 17502048 Araldite (Durcupan 4 components) Sigma Aldrich Cat# 44611-44614 Carestream Autoradiography Emulsion, Type NTB Carestream Cat# 8895666 Silver Enhancement kit Aurion, EMS Cat# 25520 TSA Plus biotin kit Akoya Biosciences Cat#NEL749A001KT Deposited data Single Cell RNA-sequencing data from the V-SVZ at P30 (Cebrian-Silla et al., 2021)24 GEO Series: GSE165555 Subseries: GSE165554 Single-cell RNA-sequencing dataset from the vSVZ (Carvajal-Ibanez et al., 2023)36 GEO: GSE197217 Single Cell RNA-sequencing data from the V-SVZ including: 1) apically labeled cells at P30, 2) lateral wall and wedge microdissections at P30 and 3) lateral wall microdissections at P365. .. Data generated in this publication GEO: GSE282956 Experimental models: Organisms/strains CD1 Charles River Laboratories Cat#022 C57BL/6 J The Jackson Laboratories Cat#000664, RRID:IMSR_JAX:000664 hGFAP:GFP The Jackson Laboratory86 Cat#003257, RRID:IMSR_JAX:003257 hGFAP:CreERt2 MMRRC Cat#016992-MU,RRID:MMRRC_016992-MU EGFP The Jackson Laboratory Cat#006567, RRID:IMSR_JAX:006567 Ai14:Rosa-CAG-LSL-tdTomato-WPRE mice The Jackson Laboratory Cat#007908, RRID:IMSR_JAX:007908 Software and Software:Article Title: Neural stem cell relay from B1 to B2 cells in the adult mouse ventricular-subventricular zone. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mm-Zeb1-C1 ADCBio Cat#451201 Brdu Sigma-Aldrich Cat# B5002 Tamoxifen Sigma-Aldrich Cat# T5648 Violet Cell Trace Thermo Fisher Scientific Cat#C34557 40,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) Sigma-Aldrich Cat# D9542 3H-thymidine Perkin Elmer Cat# 17502048 Araldite (Durcupan 4 components) Sigma Aldrich Cat# 44611-44614 Carestream Autoradiography Emulsion, Type NTB Carestream Cat# 8895666 Silver Enhancement kit Aurion, EMS Cat# 25520 TSA Plus biotin kit Akoya Biosciences Cat#NEL749A001KT Deposited data Single Cell RNA-sequencing data from the V-SVZ at P30 (Cebrian-Silla et al., 2021)24 GEO Series: GSE165555 Subseries: GSE165554 Single-cell RNA-sequencing dataset from the vSVZ (Carvajal-Ibanez et al., 2023)36 GEO: GSE197217 Single Cell RNA-sequencing data from the V-SVZ including: 1) apically labeled cells at P30, 2) lateral wall and wedge microdissections at P30 and 3) lateral wall microdissections at P365. .. Data generated in this publication GEO: GSE282956 Experimental models: Organisms/strains CD1 Charles River Laboratories Cat#022 C57BL/6 J The Jackson Laboratories Cat#000664, RRID:IMSR_JAX:000664 hGFAP:GFP The Jackson Laboratory86 Cat#003257, RRID:IMSR_JAX:003257 hGFAP:CreERt2 MMRRC Cat#016992-MU,RRID:MMRRC_016992-MU EGFP The Jackson Laboratory Cat#006567, RRID:IMSR_JAX:006567 Ai14:Rosa-CAG-LSL-tdTomato-WPRE mice The Jackson Laboratory Cat#007908, RRID:IMSR_JAX:007908 Software and Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and Recombinant:Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and High Performance Liquid Chromatography:Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and Acid Assay:Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and Enzyme-linked Immunosorbent Assay:Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and Cholesterol Assay:Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and cDNA Synthesis:Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and SYBR Green Assay:Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and Magnetic Resonance Imaging:Article Title: Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Peptide G108 WuXi AppTec N/A Glucagon Wuxi AppTec N/A GLP-1(7-36)NH2 Wuxi AppTec N/A Ethanol Sigma-Aldrich Cat# 8187601000 2-propanol Sigma-Aldrich Cat# I9516-1L Lipofectamine 2000 Invitrogen Cat# 11668-019 Tel Test Inc RNA STAT 60 Fisher Scientific Cat# NC9256697 Oil Red O Sigma-Aldrich Cat# O0625-100G HPLC grade water, Alfa Aesar, ultrapure Fisher Scientific Cat# 11338217 Critical commercial assays cAMP – GS dynamic kit Cisbio Cat# 62AM4PEC Total amino acid assay kit Sigma-Aldrich, Abcam Cat# MAK002, ab65347 FGF21 ELISA kit Abcam Cat# ab229382 Triglyceride assay kit Abcam Cat# ab65336 Cholesterol assay kit Abcam Cat# ab65390 Leptin ELISA kit Abcam Cat# ab199082 Iscript cDNA synthesis kit Bio-rad Cat# 1708891 iTaq universal SYBRG Supermix Bio-rad Cat# 1725121 Experimental models: Cell lines HEK293T cells European Collection of Authenticated Cell Cultures (ECACC) RRID:CVCL_0063 Oligonucleotides GCG receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57952-UT GLP-1 receptor cDNA ORF Clone, Mouse, untagged Sinobiological Cat# MG57391-UT KiCqStart SYBR Green Primers Merck https://www.sigmaaldrich.com Experimental models: Organisms/strains C57BL/6 mice Charles River Cat# C57BL/6NCrl GcgrDliver mice Jackson Laboratories N/A Fgf21Dliver mice Jackson Laboratories N/A Software and statistical analysis:Article Title: EDEM3 Modulates Plasma Triglyceride Level through Its Regulation of LRP1 Expression Article Snippet: .. Key resources table Reagents or Resources Source Identifier Antibodies Mouse monoclonal against EDEM3 Mouse monoclonal against β-actin Rabbit monoclonal against LRP1 Rabbit monoclonal against GAPDH Sigma-Aldrich Santa Cruz Abcam Cell Signaling E0409 sc-47778 ab92544 3683S Chemicals, reagents Protease inhibitor cocktail IGPAL-CA-630 Tris buffered saline (10X, pH 7.4) Doxycycline Tunicamycin FBS (heat inactivated) Puromycin NP-40 substitute Paraformaldehyde (4%) Sodium chloride (5M) Tris-HCl buffer (1M, pH 7.5) Dil-LDL Dil-VLDL Fluorescein-labeled ConA Isopropanol (for metabolomics) Methanol (for metabolomics) Roche Sigma-Aldrich Boston BioProducts Sigma-Aldrich Sigma-Aldrich SAFC Sigma-Aldrich Sigma-Aldrich Santa Cruz Boston BioProducts Boston BioProducts Alfa Aesar Alfa Aesar Vector laboratories Fisher Chemical Fisher Chemical 05892791001 I8896 BM-300 D1822 T7765 12306C SBR00017 I8896 Sc-281692 BM-244 BM-315 BT-904 BT-922 FL1001 BP2632-4 A456-4 sCommercial assays ApoB ELISA kit Triglyceride colorimetric assay kit RNeasy Mini Kit MABTECH Cayman Qiagen 3715-1H-6 10010303 74104 alamarBlue reagent Quickchange site-directed mutagenesis Gateway BP clonase II enzyme mix Gateway LR clonase II enzyme mix Fisher Scientific Agilent Technologies Introgen Introgen DAL1025 200519-5 11789-020 11791-020 Vectors, cDNA WT EDEM3 cDNA lentiGuide-Puro FU-tetO-Gateway pCAG-EGXXFP pX602 Genescript addgene addgene addgene addgene OHu101917 52963 43914 50716 61593 Core facility services WT and P746S EDEM3 Lentivirus preparation EDEM3 CRISPR AAV preparation Flow cytometry RNA-seq analysis Metabolomics analysis Boston Children’s Hospital viral core Penn Vector Core MGH HSCI-CRM Flow Cytometry Core Facility Partners HealthCare Translational Genomics Core Broad Institute metabolomics platform Custom Custom Mouse strain and diet C57BL/6J High fat diet The Jackson Lab Envigo 000664 TD.06414 Software and quantification GraphPad Prism 7 ImageJ software GraphPad software ImageJ http://graphpad.com http://imageJ.net .. image analysis:Article Title: EDEM3 Modulates Plasma Triglyceride Level through Its Regulation of LRP1 Expression Article Snippet: .. Key resources table Reagents or Resources Source Identifier Antibodies Mouse monoclonal against EDEM3 Mouse monoclonal against β-actin Rabbit monoclonal against LRP1 Rabbit monoclonal against GAPDH Sigma-Aldrich Santa Cruz Abcam Cell Signaling E0409 sc-47778 ab92544 3683S Chemicals, reagents Protease inhibitor cocktail IGPAL-CA-630 Tris buffered saline (10X, pH 7.4) Doxycycline Tunicamycin FBS (heat inactivated) Puromycin NP-40 substitute Paraformaldehyde (4%) Sodium chloride (5M) Tris-HCl buffer (1M, pH 7.5) Dil-LDL Dil-VLDL Fluorescein-labeled ConA Isopropanol (for metabolomics) Methanol (for metabolomics) Roche Sigma-Aldrich Boston BioProducts Sigma-Aldrich Sigma-Aldrich SAFC Sigma-Aldrich Sigma-Aldrich Santa Cruz Boston BioProducts Boston BioProducts Alfa Aesar Alfa Aesar Vector laboratories Fisher Chemical Fisher Chemical 05892791001 I8896 BM-300 D1822 T7765 12306C SBR00017 I8896 Sc-281692 BM-244 BM-315 BT-904 BT-922 FL1001 BP2632-4 A456-4 sCommercial assays ApoB ELISA kit Triglyceride colorimetric assay kit RNeasy Mini Kit MABTECH Cayman Qiagen 3715-1H-6 10010303 74104 alamarBlue reagent Quickchange site-directed mutagenesis Gateway BP clonase II enzyme mix Gateway LR clonase II enzyme mix Fisher Scientific Agilent Technologies Introgen Introgen DAL1025 200519-5 11789-020 11791-020 Vectors, cDNA WT EDEM3 cDNA lentiGuide-Puro FU-tetO-Gateway pCAG-EGXXFP pX602 Genescript addgene addgene addgene addgene OHu101917 52963 43914 50716 61593 Core facility services WT and P746S EDEM3 Lentivirus preparation EDEM3 CRISPR AAV preparation Flow cytometry RNA-seq analysis Metabolomics analysis Boston Children’s Hospital viral core Penn Vector Core MGH HSCI-CRM Flow Cytometry Core Facility Partners HealthCare Translational Genomics Core Broad Institute metabolomics platform Custom Custom Mouse strain and diet C57BL/6J High fat diet The Jackson Lab Envigo 000664 TD.06414 Software and quantification GraphPad Prism 7 |