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professional quantitative colocalization analysis software nikon a1  (Nikon)


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    Nikon professional quantitative colocalization analysis software nikon a1
    Professional Quantitative Colocalization Analysis Software Nikon A1, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software+colocalization+analysis/pm40574328-284-9-14
    Average 90 stars, based on 1 article reviews
    professional quantitative colocalization analysis software nikon a1 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Software:

    Article Title: Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection
    Article Snippet: .. The colocalization coefficients were calculated using professional quantitative colocalization analysis software (Nikon A1, Nikon, Japan) included with a Nikon A1 confocal microscope and expressed as a Pearson’s correlation coefficient. ..

    Article Title: Biomarker-activated multifunctional lysosome-targeting chimeras mediated selective degradation of extracellular amyloid fibrils
    Article Snippet: targeting chimeras mediated selective degradation of extracellular amyloid fibrils Zhenqi Liu, Qingqing Deng, Geng Qin, Jie Yang, Haochen Zhang, Jinsong Ren, Xiaogang Qu xqu@ciac.ac.cn Highlights Ab-Cu complexes can be leveraged to activate cli-LYTACs in AD lesion areas Cli-LYTACs promoted Ab degradation through the endolysosomal pathway Cli-LYTACs recognize polymorphic aggregates and facilitate their degradation Polydopamine potentiates LYTAC-mediated Ab degradation We design, synthesize, and select Pittsburgh compound B (PiB)-derived suitable chimera molecules and then construct multifunctional polydopamine-based LYTACs (KPLYs) with blood-brain barrier permeability and activatable Ab degradation ability used for Alzheimer’s disease treatment.. Liu et al., Chem 9, 2016–2038 July 13, 2023 a 2023 Elsevier Inc. https://doi.org/10.1016/j.chempr.2023.06.003 ll

    Article Title: Enantioselective Degrader for Elimination of Extracellular Aggregation-Prone Proteins hIAPP Associated with Type 2 Diabetes.
    Article Snippet: .. Scale bars are 10 μm. (c) The percentage of hIAPP peptides that are colocalized with lysosomes in each sample (n = 2, independent experiments) was analyzed by Nikon colocalization software. ..

    Article Title: The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly.
    Article Snippet: Next, to investigate the relationship between Rab14 and NS5A, cells transfected with the indicated constructs (pFlag-NS5A, NS5A-1, -2 or -3) for 48 hpt were fixed and subjected to confocal microscopy using mouse anti-Flag antibody (Invitrogen, Carlsbad, USA) or rabbit anti-Rab14 antibody (Proteintech, Westlake Village, USA). .. The colocalization coefficients were calculated using the professional quantitative colocalization analysis software (Nikon A1, Nikon, Japan) included with a Nikon A1 confocal microscope and expressed as a Pearson’s correlation coefficient. ..

    Article Title: Targeting mitochondrial degradation by chimeric autophagy-tethering compounds
    Article Snippet: .. The percentage of mitochondria that were colocalized with lysosomes was analyzed by Nikon colocalization software and shown in right. (b and c). ..

    Article Title: Valosin-containing protein (VCP/p97) is responsible for the endocytotic trafficking of classical swine fever virus.
    Article Snippet: Classical swine fever virus (CSFV), a member of the Flaviviridae enveloped RNA virus family, results in an epidemic disease that brings serious economic losses to the pig industry worldwide.. Valosin-containing protein (VCP/p97), a multifunctional active protein in cells, is related to the life activities of many viruses.. However, the role of VCP in CSFV infection remains unknown.

    Article Title: Intracellular Vimentin Regulates the Formation of Classical Swine Fever Virus Replication Complex through Interaction with NS5A Protein.
    Article Snippet: .. For confocal microscopy, the colocalization coefficients were calculated using professional quantitative colocalization analysis software (Nikon A1; Nikon), included with a Nikon A1 confocal microscope and are expressed as a Pearson's correlation coefficient. .. For IFA, cells on 24-well plates were examined under a Zeiss LSM700 confocal microscope.

    Article Title: Classical swine fever virus hijacks ESCRT-III and VPS4A to promote phagophore closure for accelerating mitophagy.
    Article Snippet: Classical swine fever virus (CSFV) infection induces complete mitophagy, which is essential for the clearance of damaged mitochondria.. The endosomal sorting complex required for transport (ESCRT) machinery plays a vital role in mediating phagophore closure and autophagosome-lysosome fusion during starvation-induced autophagy.. Nevertheless, its involvement in CSFV-induced mitophagy and the underlying mechanisms remain insufficiently understood.

    Microscopy:

    Article Title: Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection
    Article Snippet: .. The colocalization coefficients were calculated using professional quantitative colocalization analysis software (Nikon A1, Nikon, Japan) included with a Nikon A1 confocal microscope and expressed as a Pearson’s correlation coefficient. ..

    Article Title: The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly.
    Article Snippet: Next, to investigate the relationship between Rab14 and NS5A, cells transfected with the indicated constructs (pFlag-NS5A, NS5A-1, -2 or -3) for 48 hpt were fixed and subjected to confocal microscopy using mouse anti-Flag antibody (Invitrogen, Carlsbad, USA) or rabbit anti-Rab14 antibody (Proteintech, Westlake Village, USA). .. The colocalization coefficients were calculated using the professional quantitative colocalization analysis software (Nikon A1, Nikon, Japan) included with a Nikon A1 confocal microscope and expressed as a Pearson’s correlation coefficient. ..

    Article Title: Intracellular Vimentin Regulates the Formation of Classical Swine Fever Virus Replication Complex through Interaction with NS5A Protein.
    Article Snippet: .. For confocal microscopy, the colocalization coefficients were calculated using professional quantitative colocalization analysis software (Nikon A1; Nikon), included with a Nikon A1 confocal microscope and are expressed as a Pearson's correlation coefficient. .. For IFA, cells on 24-well plates were examined under a Zeiss LSM700 confocal microscope.

    Article Title: Classical swine fever virus hijacks ESCRT-III and VPS4A to promote phagophore closure for accelerating mitophagy.
    Article Snippet: Classical swine fever virus (CSFV) infection induces complete mitophagy, which is essential for the clearance of damaged mitochondria.. The endosomal sorting complex required for transport (ESCRT) machinery plays a vital role in mediating phagophore closure and autophagosome-lysosome fusion during starvation-induced autophagy.. Nevertheless, its involvement in CSFV-induced mitophagy and the underlying mechanisms remain insufficiently understood.

    Confocal Microscopy:

    Article Title: Intracellular Vimentin Regulates the Formation of Classical Swine Fever Virus Replication Complex through Interaction with NS5A Protein.
    Article Snippet: .. For confocal microscopy, the colocalization coefficients were calculated using professional quantitative colocalization analysis software (Nikon A1; Nikon), included with a Nikon A1 confocal microscope and are expressed as a Pearson's correlation coefficient. .. For IFA, cells on 24-well plates were examined under a Zeiss LSM700 confocal microscope.

    Article Title: Classical swine fever virus hijacks ESCRT-III and VPS4A to promote phagophore closure for accelerating mitophagy.
    Article Snippet: Classical swine fever virus (CSFV) infection induces complete mitophagy, which is essential for the clearance of damaged mitochondria.. The endosomal sorting complex required for transport (ESCRT) machinery plays a vital role in mediating phagophore closure and autophagosome-lysosome fusion during starvation-induced autophagy.. Nevertheless, its involvement in CSFV-induced mitophagy and the underlying mechanisms remain insufficiently understood.



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    A. HEK293 cells (wild-type BRAF). B. Mel-9 cells (NRAS Q61R , wild-type BRAF). C. SKMEL-239 cells (heterozygous for BRAF V600E ). D. A375 cells (homozygous for BRAF V600E ). E. Quantification of <t>colocalization</t> of BRAF and TP53 in the cell lines listed above (quantification of n=4-5 fields of view from each of n=3 biological replicates; one-way ANOVA with multiple comparisons. **** = adjusted p-valued <0.0001). F. Quantification of TP53 localization in the nucleus by DAPI staining in the cell lines listed above (quantification of n=4-5 fields of view from each of n=3 biological replicates; one-way ANOVA with multiple comparisons. * = adjusted p-valued <0.03, *** = adjusted p-value <0.0009). Immunofluorescence microscopy depicting TP53 (magenta) and BRAF (green) localization in: G. Primary epidermal melanocytes expressing wild-type BRAF with no doxycycline. H. Primary epidermal melanocytes expressing BRAF V600E with the addition of doxycycline. I. Quantification of TP53 and BRAF colocalization in the epidermal melanocytes (quantification of n=4-5 fields of view from each of n=3 biological replicates; unpaired t-test p-value <0.0001). J. Quantification of TP53 localization in the nucleus by DAPI staining (quantification of n=4-5 fields of view from each of n=3 biological replicates; unpaired t-test p-value <0.0001). K. Immunoblots detecting BRAF and TP53 localization in the cytoplasm vs. nucleus after cell fractionation of SKMEL-239 cells, A375 cells, and HEK293 cells. L. Immunoblots detecting BRAF and TP53 localization in the cytoplasm vs. nucleus after cell fractionation of primary epidermal melanocytes with and without the addition of doxycycline to turn on BRAF V600E expression.
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    Coexistence of tauopathy (hTau and pTau 181) in selected ChAT + cell clusters within BF of 3xTgAD mice . ( A ) Representative image showing mutant hTau (cyan) deposition in selected ChAT + cell cluster (magenta) at HDB on a sagittal section of a young 3xTgAD mouse (6 M). ( B ) Scatterplot of pixel intensities of cyan (quadrant 1), magenta (quadrant 2) and <t>colocalization</t> pixels (quadrant 3). ( C–D ) Higher magnification image showing colocalization of hTau and ChAT + at HDB and its colocalization pixels at quadrant 3 only on scatterplot. Pearson’s correlation coefficient (R p ) = 0.42 (ROI in circle). ( E–F ) Representative image on hTau (cyan) and ChAT + (magenta) expressing at BF from an old 3xTgAD mouse(18 M) and its scatterplot; R p = 0.20 on ROI. ( G ) Representative image on hTau deposition within ChAT + cell clusters at nBM and SI from a young 3xTgAD mice (9 M), R p = 0.19. ( H) Representative image on phosphor-tau Thr181 (cyan) deposition within ChAT + cell clusters at VDB in an old 3xTgAD mouse (20 M), R p = 0.22. ( I ) Average R p on colocalization of hTau and ChAT + cells at ROI within BF from multiple brain sections of young and old 3xTgAD mice (each dot represents one ROI). The average R p was higher in young versus in old AD mice. P = 0.004, unpaired 2-tailed t -test with equal variance, t = 3.205. df = 27; F = 1.476, P = 0.471. n = 3–5 mice/group. Abbreviations: R p : Pearson’s correlation coefficient. ROI: region of interesting. nBM: nucleus Basalis of Meynert; HDB: nucleus of horizontal limb diagonal band of Broca; VDB: nucleus of vert limb diagonal band. Scale bar, 200μm in panel A ; 100μm in panel C , E , G and H .
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    Coexistence of tauopathy (hTau and pTau 181) in selected ChAT + cell clusters within BF of 3xTgAD mice . ( A ) Representative image showing mutant hTau (cyan) deposition in selected ChAT + cell cluster (magenta) at HDB on a sagittal section of a young 3xTgAD mouse (6 M). ( B ) Scatterplot of pixel intensities of cyan (quadrant 1), magenta (quadrant 2) and <t>colocalization</t> pixels (quadrant 3). ( C–D ) Higher magnification image showing colocalization of hTau and ChAT + at HDB and its colocalization pixels at quadrant 3 only on scatterplot. Pearson’s correlation coefficient (R p ) = 0.42 (ROI in circle). ( E–F ) Representative image on hTau (cyan) and ChAT + (magenta) expressing at BF from an old 3xTgAD mouse(18 M) and its scatterplot; R p = 0.20 on ROI. ( G ) Representative image on hTau deposition within ChAT + cell clusters at nBM and SI from a young 3xTgAD mice (9 M), R p = 0.19. ( H) Representative image on phosphor-tau Thr181 (cyan) deposition within ChAT + cell clusters at VDB in an old 3xTgAD mouse (20 M), R p = 0.22. ( I ) Average R p on colocalization of hTau and ChAT + cells at ROI within BF from multiple brain sections of young and old 3xTgAD mice (each dot represents one ROI). The average R p was higher in young versus in old AD mice. P = 0.004, unpaired 2-tailed t -test with equal variance, t = 3.205. df = 27; F = 1.476, P = 0.471. n = 3–5 mice/group. Abbreviations: R p : Pearson’s correlation coefficient. ROI: region of interesting. nBM: nucleus Basalis of Meynert; HDB: nucleus of horizontal limb diagonal band of Broca; VDB: nucleus of vert limb diagonal band. Scale bar, 200μm in panel A ; 100μm in panel C , E , G and H .
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    A. HEK293 cells (wild-type BRAF). B. Mel-9 cells (NRAS Q61R , wild-type BRAF). C. SKMEL-239 cells (heterozygous for BRAF V600E ). D. A375 cells (homozygous for BRAF V600E ). E. Quantification of colocalization of BRAF and TP53 in the cell lines listed above (quantification of n=4-5 fields of view from each of n=3 biological replicates; one-way ANOVA with multiple comparisons. **** = adjusted p-valued <0.0001). F. Quantification of TP53 localization in the nucleus by DAPI staining in the cell lines listed above (quantification of n=4-5 fields of view from each of n=3 biological replicates; one-way ANOVA with multiple comparisons. * = adjusted p-valued <0.03, *** = adjusted p-value <0.0009). Immunofluorescence microscopy depicting TP53 (magenta) and BRAF (green) localization in: G. Primary epidermal melanocytes expressing wild-type BRAF with no doxycycline. H. Primary epidermal melanocytes expressing BRAF V600E with the addition of doxycycline. I. Quantification of TP53 and BRAF colocalization in the epidermal melanocytes (quantification of n=4-5 fields of view from each of n=3 biological replicates; unpaired t-test p-value <0.0001). J. Quantification of TP53 localization in the nucleus by DAPI staining (quantification of n=4-5 fields of view from each of n=3 biological replicates; unpaired t-test p-value <0.0001). K. Immunoblots detecting BRAF and TP53 localization in the cytoplasm vs. nucleus after cell fractionation of SKMEL-239 cells, A375 cells, and HEK293 cells. L. Immunoblots detecting BRAF and TP53 localization in the cytoplasm vs. nucleus after cell fractionation of primary epidermal melanocytes with and without the addition of doxycycline to turn on BRAF V600E expression.

    Journal: bioRxiv

    Article Title: Characterization of the BRAF interactome identifies BRAF V600E <=>TP53 interaction in melanoma

    doi: 10.1101/2025.06.20.660711

    Figure Lengend Snippet: A. HEK293 cells (wild-type BRAF). B. Mel-9 cells (NRAS Q61R , wild-type BRAF). C. SKMEL-239 cells (heterozygous for BRAF V600E ). D. A375 cells (homozygous for BRAF V600E ). E. Quantification of colocalization of BRAF and TP53 in the cell lines listed above (quantification of n=4-5 fields of view from each of n=3 biological replicates; one-way ANOVA with multiple comparisons. **** = adjusted p-valued <0.0001). F. Quantification of TP53 localization in the nucleus by DAPI staining in the cell lines listed above (quantification of n=4-5 fields of view from each of n=3 biological replicates; one-way ANOVA with multiple comparisons. * = adjusted p-valued <0.03, *** = adjusted p-value <0.0009). Immunofluorescence microscopy depicting TP53 (magenta) and BRAF (green) localization in: G. Primary epidermal melanocytes expressing wild-type BRAF with no doxycycline. H. Primary epidermal melanocytes expressing BRAF V600E with the addition of doxycycline. I. Quantification of TP53 and BRAF colocalization in the epidermal melanocytes (quantification of n=4-5 fields of view from each of n=3 biological replicates; unpaired t-test p-value <0.0001). J. Quantification of TP53 localization in the nucleus by DAPI staining (quantification of n=4-5 fields of view from each of n=3 biological replicates; unpaired t-test p-value <0.0001). K. Immunoblots detecting BRAF and TP53 localization in the cytoplasm vs. nucleus after cell fractionation of SKMEL-239 cells, A375 cells, and HEK293 cells. L. Immunoblots detecting BRAF and TP53 localization in the cytoplasm vs. nucleus after cell fractionation of primary epidermal melanocytes with and without the addition of doxycycline to turn on BRAF V600E expression.

    Article Snippet: Colocalization was quantified on a Lecia confocal microscope using the Leica LasX colocalization analysis (version 3.5.7.23225) software to quantify the overlap between fluorescent signals.

    Techniques: Staining, Immunofluorescence, Microscopy, Expressing, Western Blot, Cell Fractionation

    Coexistence of tauopathy (hTau and pTau 181) in selected ChAT + cell clusters within BF of 3xTgAD mice . ( A ) Representative image showing mutant hTau (cyan) deposition in selected ChAT + cell cluster (magenta) at HDB on a sagittal section of a young 3xTgAD mouse (6 M). ( B ) Scatterplot of pixel intensities of cyan (quadrant 1), magenta (quadrant 2) and colocalization pixels (quadrant 3). ( C–D ) Higher magnification image showing colocalization of hTau and ChAT + at HDB and its colocalization pixels at quadrant 3 only on scatterplot. Pearson’s correlation coefficient (R p ) = 0.42 (ROI in circle). ( E–F ) Representative image on hTau (cyan) and ChAT + (magenta) expressing at BF from an old 3xTgAD mouse(18 M) and its scatterplot; R p = 0.20 on ROI. ( G ) Representative image on hTau deposition within ChAT + cell clusters at nBM and SI from a young 3xTgAD mice (9 M), R p = 0.19. ( H) Representative image on phosphor-tau Thr181 (cyan) deposition within ChAT + cell clusters at VDB in an old 3xTgAD mouse (20 M), R p = 0.22. ( I ) Average R p on colocalization of hTau and ChAT + cells at ROI within BF from multiple brain sections of young and old 3xTgAD mice (each dot represents one ROI). The average R p was higher in young versus in old AD mice. P = 0.004, unpaired 2-tailed t -test with equal variance, t = 3.205. df = 27; F = 1.476, P = 0.471. n = 3–5 mice/group. Abbreviations: R p : Pearson’s correlation coefficient. ROI: region of interesting. nBM: nucleus Basalis of Meynert; HDB: nucleus of horizontal limb diagonal band of Broca; VDB: nucleus of vert limb diagonal band. Scale bar, 200μm in panel A ; 100μm in panel C , E , G and H .

    Journal: Brain Communications

    Article Title: Chronic inflammation with microglia senescence at basal forebrain: impact on cholinergic deficit in Alzheimer’s brain haemodynamics

    doi: 10.1093/braincomms/fcae204

    Figure Lengend Snippet: Coexistence of tauopathy (hTau and pTau 181) in selected ChAT + cell clusters within BF of 3xTgAD mice . ( A ) Representative image showing mutant hTau (cyan) deposition in selected ChAT + cell cluster (magenta) at HDB on a sagittal section of a young 3xTgAD mouse (6 M). ( B ) Scatterplot of pixel intensities of cyan (quadrant 1), magenta (quadrant 2) and colocalization pixels (quadrant 3). ( C–D ) Higher magnification image showing colocalization of hTau and ChAT + at HDB and its colocalization pixels at quadrant 3 only on scatterplot. Pearson’s correlation coefficient (R p ) = 0.42 (ROI in circle). ( E–F ) Representative image on hTau (cyan) and ChAT + (magenta) expressing at BF from an old 3xTgAD mouse(18 M) and its scatterplot; R p = 0.20 on ROI. ( G ) Representative image on hTau deposition within ChAT + cell clusters at nBM and SI from a young 3xTgAD mice (9 M), R p = 0.19. ( H) Representative image on phosphor-tau Thr181 (cyan) deposition within ChAT + cell clusters at VDB in an old 3xTgAD mouse (20 M), R p = 0.22. ( I ) Average R p on colocalization of hTau and ChAT + cells at ROI within BF from multiple brain sections of young and old 3xTgAD mice (each dot represents one ROI). The average R p was higher in young versus in old AD mice. P = 0.004, unpaired 2-tailed t -test with equal variance, t = 3.205. df = 27; F = 1.476, P = 0.471. n = 3–5 mice/group. Abbreviations: R p : Pearson’s correlation coefficient. ROI: region of interesting. nBM: nucleus Basalis of Meynert; HDB: nucleus of horizontal limb diagonal band of Broca; VDB: nucleus of vert limb diagonal band. Scale bar, 200μm in panel A ; 100μm in panel C , E , G and H .

    Article Snippet: For colocalization evaluation with double/triple immunohistochemistry staining on brain sections, Pearson’s correlation coefficient ( R p ) evaluation was applied using Zeiss confocal microscope (LSM710) ZEN software on ROI (region of interesting) from multiple images for statistical analysis (ZEISS: Acquiring and Analysing Data for Colocalization Experiments in AIM or ZEN Software).

    Techniques: Mutagenesis, Expressing

    Iba1 + microglia interacting tauopathy with phenotypic changes in BF of 3xTgAD mice. ( A–B ) Representative images show Iba1 + microglia (magenta) at BF of an old WT mouse (2y) and scatterplot of colocalization pixels of Iba1(magenta) and hTau (cyan), which was almost undetectable in WT mice, R p = 0.03. ( C–D ) Representative images reveal Iba1 + microglia (magenta) were interacting hTau (cyan) within BF of a 3xTgAD mouse (14 M) and scatterplot on colocalization pixels at ROI. R p = 0.31. ( E ) Internalization, engulfing or phagocytosing hTau by Iba1 + microglia at BF of an old 3xTgAD mouse (2y). R p = 0.61. Microglia also showed heterogeneous morphology. ( F ) The average % of microglia associated with tauopathy (including phagocytosis) at BF was higher in 3xTgAD mice versus Age-matched WT mice. One-way ANOVA, F = 7.105, P = 0.005. Multiple comparison using unpaired 2-tailed t -test with equal variance between young WT and 3xTgAD mice, P = 0.013, t = 3.32, df =7; between old WT and 3xTgAD mice, P = 0.02, t = 3.5, df =5; n = 3–5 mice/group. ( G ) The average R p on colocalization of Iba1 and tauopathy at ROIs within BF from multiple brain sections of old 3xTgAD and WT mice, P < 0.0001, unpaired 2-tailed t -test with equal variance; t = 4.634, df =29. F = 1.567, P = 0.414. n = 3–5 mice/group. Scale bar, 100μm in panels 5A-a, c, e.

    Journal: Brain Communications

    Article Title: Chronic inflammation with microglia senescence at basal forebrain: impact on cholinergic deficit in Alzheimer’s brain haemodynamics

    doi: 10.1093/braincomms/fcae204

    Figure Lengend Snippet: Iba1 + microglia interacting tauopathy with phenotypic changes in BF of 3xTgAD mice. ( A–B ) Representative images show Iba1 + microglia (magenta) at BF of an old WT mouse (2y) and scatterplot of colocalization pixels of Iba1(magenta) and hTau (cyan), which was almost undetectable in WT mice, R p = 0.03. ( C–D ) Representative images reveal Iba1 + microglia (magenta) were interacting hTau (cyan) within BF of a 3xTgAD mouse (14 M) and scatterplot on colocalization pixels at ROI. R p = 0.31. ( E ) Internalization, engulfing or phagocytosing hTau by Iba1 + microglia at BF of an old 3xTgAD mouse (2y). R p = 0.61. Microglia also showed heterogeneous morphology. ( F ) The average % of microglia associated with tauopathy (including phagocytosis) at BF was higher in 3xTgAD mice versus Age-matched WT mice. One-way ANOVA, F = 7.105, P = 0.005. Multiple comparison using unpaired 2-tailed t -test with equal variance between young WT and 3xTgAD mice, P = 0.013, t = 3.32, df =7; between old WT and 3xTgAD mice, P = 0.02, t = 3.5, df =5; n = 3–5 mice/group. ( G ) The average R p on colocalization of Iba1 and tauopathy at ROIs within BF from multiple brain sections of old 3xTgAD and WT mice, P < 0.0001, unpaired 2-tailed t -test with equal variance; t = 4.634, df =29. F = 1.567, P = 0.414. n = 3–5 mice/group. Scale bar, 100μm in panels 5A-a, c, e.

    Article Snippet: For colocalization evaluation with double/triple immunohistochemistry staining on brain sections, Pearson’s correlation coefficient ( R p ) evaluation was applied using Zeiss confocal microscope (LSM710) ZEN software on ROI (region of interesting) from multiple images for statistical analysis (ZEISS: Acquiring and Analysing Data for Colocalization Experiments in AIM or ZEN Software).

    Techniques: Comparison

    Increased expression of the senescence marker p16 INK4A in Iba1 + microglia and hTau harbouring cells with aging in BF of 3xTgAD mice . ( A–B ) Representative Immunofluorescence images revealed cell senescence marker p16 INK4A (cyan) expressed in Iba1 + (magenta) microglia within BF of aged 3xTgAD mice ( R p = 0.67), compared to aged WT mice ( R p = 0.21). ( C ) p16 INK4A expression (cyan) was also observed in hTau-bearing (magenta) cells within BF of 3xTgAD mice (R p = 0.37). ( D ) The Iba1 + microglia expressing p16 INK4A (%) were evaluated within four groups, one-way ANOVA: F = 18.99, P < 0.0001. It was significantly higher in old versus in younger AD mice, P = 0.0007, t = 5.755, df = 7, and higher in old AD versus old WT mice, P = 0.001, t = 5.117, df = 7; unpaired 2-tailed t -test with equal variance, The data points were mean on 3–4 brain sections/each mouse, n = 3–5 mice/group. ( E ) The average R p on colocalization of Iba1 with p16 INK4A (ROIs) from multiple sections of WT and 3xTgAD mice in two age groups was compared. One-way ANOVA analysis: F = 51.36, P < 0.001. The average R p was significantly higher in old AD versus in young AD mice, and in old AD versus old WT mice, but was not significantly different between young AD versus young WT mice as indicated. Tukey’s multiple comparisons test with adjusted values of P < 0.01 was indicated, df = 32; n = 3–4 mice/group. ( F ) The average R p on colocalization of p16 INK4A or TNF-α expression in hTau-bearing cells at BF of old AD mice. Scale bar, 50μm in panels A–C ; BF, Basal forebrain.

    Journal: Brain Communications

    Article Title: Chronic inflammation with microglia senescence at basal forebrain: impact on cholinergic deficit in Alzheimer’s brain haemodynamics

    doi: 10.1093/braincomms/fcae204

    Figure Lengend Snippet: Increased expression of the senescence marker p16 INK4A in Iba1 + microglia and hTau harbouring cells with aging in BF of 3xTgAD mice . ( A–B ) Representative Immunofluorescence images revealed cell senescence marker p16 INK4A (cyan) expressed in Iba1 + (magenta) microglia within BF of aged 3xTgAD mice ( R p = 0.67), compared to aged WT mice ( R p = 0.21). ( C ) p16 INK4A expression (cyan) was also observed in hTau-bearing (magenta) cells within BF of 3xTgAD mice (R p = 0.37). ( D ) The Iba1 + microglia expressing p16 INK4A (%) were evaluated within four groups, one-way ANOVA: F = 18.99, P < 0.0001. It was significantly higher in old versus in younger AD mice, P = 0.0007, t = 5.755, df = 7, and higher in old AD versus old WT mice, P = 0.001, t = 5.117, df = 7; unpaired 2-tailed t -test with equal variance, The data points were mean on 3–4 brain sections/each mouse, n = 3–5 mice/group. ( E ) The average R p on colocalization of Iba1 with p16 INK4A (ROIs) from multiple sections of WT and 3xTgAD mice in two age groups was compared. One-way ANOVA analysis: F = 51.36, P < 0.001. The average R p was significantly higher in old AD versus in young AD mice, and in old AD versus old WT mice, but was not significantly different between young AD versus young WT mice as indicated. Tukey’s multiple comparisons test with adjusted values of P < 0.01 was indicated, df = 32; n = 3–4 mice/group. ( F ) The average R p on colocalization of p16 INK4A or TNF-α expression in hTau-bearing cells at BF of old AD mice. Scale bar, 50μm in panels A–C ; BF, Basal forebrain.

    Article Snippet: For colocalization evaluation with double/triple immunohistochemistry staining on brain sections, Pearson’s correlation coefficient ( R p ) evaluation was applied using Zeiss confocal microscope (LSM710) ZEN software on ROI (region of interesting) from multiple images for statistical analysis (ZEISS: Acquiring and Analysing Data for Colocalization Experiments in AIM or ZEN Software).

    Techniques: Expressing, Marker, Immunofluorescence

    Persistent increase of TNF-α in SASP microglia within BF in 3xTgAD mice. Representative images showing the pro-inflammatory cytokine TNF-ɑ expressed in Iba1 + microglia and corresponding scatterplots on colocalization pixel intensity within BF of WT ( A–B ) and 3xTgAD ( C–D ) mice. ( E) Representative image showed the persistent expression of TNF-ɑ in Iba1 + microglia from a very old 3xTgAD mouse (30 M), which spread to neighbouring cells and extracellular microenvironment (Fi–iv). ( F ) The Iba1 + microglia cells with TNF-α expression also displayed phenotype and deteriorating changes. (G ) The average R p on colocalization of Iba1 with TNF-α was significantly higher in old 3xTgAD versus in old WT mice as evaluated from multiple brain sections. P < 0.001, unpaired 2-tailed t -test with equal variance, t = 6.584, df = 48; F = 1.761, P = 0.168; n = 3–5 mice/group. ( H–I ) The representative images revealed the area (%) with TNF-ɑ distribution at BF of WT (Hi–ii) and 3xTgAD (Hiii–iv) mice (16–24 months) by Image J analyses, P = 0.004; 2-tailed t -test with equal variance, t = 3.73, df = 10; F = 4.607, P = 0.119; 2–3 sections/mouse, n = 4 mice/group. Scale bars, 50μm in A & C ; 20μm in E & F ; 100μm in H; R p , Pearson’s correlation coefficient.

    Journal: Brain Communications

    Article Title: Chronic inflammation with microglia senescence at basal forebrain: impact on cholinergic deficit in Alzheimer’s brain haemodynamics

    doi: 10.1093/braincomms/fcae204

    Figure Lengend Snippet: Persistent increase of TNF-α in SASP microglia within BF in 3xTgAD mice. Representative images showing the pro-inflammatory cytokine TNF-ɑ expressed in Iba1 + microglia and corresponding scatterplots on colocalization pixel intensity within BF of WT ( A–B ) and 3xTgAD ( C–D ) mice. ( E) Representative image showed the persistent expression of TNF-ɑ in Iba1 + microglia from a very old 3xTgAD mouse (30 M), which spread to neighbouring cells and extracellular microenvironment (Fi–iv). ( F ) The Iba1 + microglia cells with TNF-α expression also displayed phenotype and deteriorating changes. (G ) The average R p on colocalization of Iba1 with TNF-α was significantly higher in old 3xTgAD versus in old WT mice as evaluated from multiple brain sections. P < 0.001, unpaired 2-tailed t -test with equal variance, t = 6.584, df = 48; F = 1.761, P = 0.168; n = 3–5 mice/group. ( H–I ) The representative images revealed the area (%) with TNF-ɑ distribution at BF of WT (Hi–ii) and 3xTgAD (Hiii–iv) mice (16–24 months) by Image J analyses, P = 0.004; 2-tailed t -test with equal variance, t = 3.73, df = 10; F = 4.607, P = 0.119; 2–3 sections/mouse, n = 4 mice/group. Scale bars, 50μm in A & C ; 20μm in E & F ; 100μm in H; R p , Pearson’s correlation coefficient.

    Article Snippet: For colocalization evaluation with double/triple immunohistochemistry staining on brain sections, Pearson’s correlation coefficient ( R p ) evaluation was applied using Zeiss confocal microscope (LSM710) ZEN software on ROI (region of interesting) from multiple images for statistical analysis (ZEISS: Acquiring and Analysing Data for Colocalization Experiments in AIM or ZEN Software).

    Techniques: Expressing