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prism 4–7  (GraphPad Software Inc)


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    GraphPad Software Inc prism 4–7
    Prism 4–7, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/software+and+algorithms/software++algorithm+prism+graphpad+versions+5+and+7/pm28259395-73-6-8
    Average 90 stars, based on 1 article reviews
    prism 4–7 - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Brain-derived neurotrophic factor expression in serotonergic neurons improves stress resilience and promotes adult hippocampal neurogenesis.
    Article Snippet: Statistical analysis was done using GraphPad Prism 4 and 7 (GraphPad Software Inc, La Jolla, CA, USA) and IBM SPSS Statistics 22 v software (IBM Corporation, Armonk, NY, USA).

    Article Title: Short communication: Tryptic β-casein hydrolysate modulates enteric nervous system development in primary culture.
    Article Snippet: Statistical analyses were carried out using Prism 4–7 (Graphpad Software, San Diego, CA).

    Article Title: Inhibition of PGE 2 /EP4 receptor signaling enhances oxaliplatin efficacy in resistant colon cancer cells through modulation of oxidative stress
    Article Snippet: Data was analyzed using the Student’s t-test or a one-way analysis of variance (ANOVA) when appropriate using GraphPad Prism (software versions 5.0c and 7).

    Software:

    Article Title: Integration of IL-2 and IL-4 signals coordinates divergent regulatory T cell responses and drives therapeutic efficacy
    Article Snippet: To obtain, contact the Cobb lab. Antibody aCD4-magnetic beads (rat, monoclonal) Miltenyi Biotec 130-049-201, RRID:AB_ 2722753 (1:50–1:100), (50 mL) Antibody aCD3 (Armenian hamster, monoclonal) eBioscience 16-0031-85, RRID: AB_ 468848 (1:400), (50 mL) Antibody aCD28 (Syrian hamster, monoclonal) eBioscience 16-0281-82, RRID: AB_ 468921 (1:400), (50 mL) Antibody CD4-PE (rat, monoclonal) eBioscience 12-0042-82, RRID: AB_ 465510 (1:250–1:500), (50 mL) Antibody CD4-AF647 (rat, monoclonal) Biolegend 100530, RRID: AB_389325 (1:250–1:500), (50 mL) Antibody CD4-BV421 (rat, monoclonal) BD 740007, RRID: AB_2739779 (1:250–1:500), (50 mL) Antibody F4/80-BV711 (rat, monoclonal) BD 565612, RRID: AB_2734769 (1:250–1:500), (50 mL) Antibody CD25-PECy7 (rat, monoclonal) BD 561780, RRID: AB_ 10893596 (1:250–1:500), (50 mL) Antibody CD25-PE (rat, monoclonal) eBioscience 12-0251-82, RRID: AB_ 465607 (1:250–1:500), (50 mL) Antibody CD124-BB700 (rat, monoclonal) BD 742172, RRID: AB_2871410 (1:250–1:500), (50 mL) Antibody CD122-APC (rat, monoclonal) BD 564924, RRID: AB_2739009 (1:250–1:500), (50 mL) Antibody CD132-BV421 (rat, monoclonal) BD 740039, RRID: AB_2739809 (1:250–1:500), (50 mL) Antibody CD213a1-Biotin (rabbit, polyclonal) MyBioSource MBS2001753 (1:250–1:500), (50 mL) Antibody pSTAT3-AF647 (mouse, monoclonal) BD 557815, RRID: AB_647144 (1:100–1:200), (25 mL) Antibody pSTAT5-AF647 (mouse, monoclonal) BD 612599, RRID: AB_399882 (1:100–1:200), (25 mL) Antibody pSTAT6-AF647 (mouse, monoclonal) BD 558242, RRID: AB_647145 (1:100–1:200), (25 mL) Antibody IL-10R biotin (rat, monoclonal) Biolegend 112704, RRID: AB_313517 (1:250–1:500), (50 mL) Antibody SA-APCefluor780 eBioscience 47-4317-82, RRID: AB_ 10366688 (1:250–1:500), (50 mL) Antibody SA-BV605 BD 563260, RRID: AB_2869476 (1:250–1:500), (50 mL) Peptide, recombinant protein Recombinant IL-2 R and D Systems 402 ML-020 Peptide, recombinant protein Recombinant IL-4 R and D Systems 404 ML-010 Peptide, recombinant protein Recombinant IL-10 R and D Systems 417 ML Commercial assay, kit IFNg ELISA MAX Biolegend 430803 Commercial assay, kit IL-4 ELISA MAX Biolegend 431102 Commercial assay, kit IL-10 ELISA MAX Biolegend 431413 Commercial assay, kit IL-17A ELISA MAX Biolegend 432501 Commercial assay, kit MOG35-55/CFA Emulsion PTX Hooke Laboratories EK-2110 Chemical compound, drug STAT5i Stemcell 73852 Continued on next page Zhou et al. eLife 2021;10:e57417. .. DOI: https://doi.org/10.7554/eLife.57417 19 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug House dust mite Greer XPB8103A2.5 Chemical compound, drug Ovalbumin Sigma-Aldrich A5503 Chemical compound, drug Cockroach antigen Greer XPB46D3A4 Chemical compound, drug Methacholine Sigma-Aldrich 62-51-1 Software, algorithm Prism Graphpad Versions 5 and 7, RRID: SCR_002798 Software, algorithm FlowJo FlowJo v10.7, RRID:SCR_008520 Other FACS-ARIA SORP BD Other Attune NxT Thermo Fisher Other Sytox Red Invitrogen S34859 Other Fixable Viability Stain 510 BD 564406 Other MOG I-A(b) tetramer-BV421 NIH Tetramer Core GWYRSPFSRVVH Other Control I-A(b) tetramer-BV421 NIH Tetramer Core PVSKMRMATPLLMQA Other CellTrace Violet Invitrogen C34557 or C34571 Other Europium-conjugated streptavidin Perkin-Elmer 1244–360 Other DELFIA Enhancement solution Perkin-Elmer 4001–0010 Other 10% formalin Anachemia 41916–700 Other Hematoxylin Ricca 3536–32 Other Eosin EMD Harleco 588X-75 Other Solvent Blue 38 Sigma-Aldrich S3382 Other Percoll GE 17-0891-02 .. C57BL/6J (Stock #000664), Il10GFP (B6.129S6-Il10tm1Flv/RthsnJ, Stock #008379), Foxp3RFP (C57BL/6Foxp3tm1Flv/J, Stock #008374), Foxp3creYFP (B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J, Stock 016959), and Il10-/- (B6.129P2-Il10tm1Cgn/J, Stock #002251) mice, all on the C57BL/6 background, as well as Foxp3GFP mice on the BALB/c background (C.Cg-Foxp3tm2Tch, Stock #006769) were purchased from the Jackson Laboratory (Bar Harbor, ME).

    FACS:

    Article Title: Integration of IL-2 and IL-4 signals coordinates divergent regulatory T cell responses and drives therapeutic efficacy
    Article Snippet: To obtain, contact the Cobb lab. Antibody aCD4-magnetic beads (rat, monoclonal) Miltenyi Biotec 130-049-201, RRID:AB_ 2722753 (1:50–1:100), (50 mL) Antibody aCD3 (Armenian hamster, monoclonal) eBioscience 16-0031-85, RRID: AB_ 468848 (1:400), (50 mL) Antibody aCD28 (Syrian hamster, monoclonal) eBioscience 16-0281-82, RRID: AB_ 468921 (1:400), (50 mL) Antibody CD4-PE (rat, monoclonal) eBioscience 12-0042-82, RRID: AB_ 465510 (1:250–1:500), (50 mL) Antibody CD4-AF647 (rat, monoclonal) Biolegend 100530, RRID: AB_389325 (1:250–1:500), (50 mL) Antibody CD4-BV421 (rat, monoclonal) BD 740007, RRID: AB_2739779 (1:250–1:500), (50 mL) Antibody F4/80-BV711 (rat, monoclonal) BD 565612, RRID: AB_2734769 (1:250–1:500), (50 mL) Antibody CD25-PECy7 (rat, monoclonal) BD 561780, RRID: AB_ 10893596 (1:250–1:500), (50 mL) Antibody CD25-PE (rat, monoclonal) eBioscience 12-0251-82, RRID: AB_ 465607 (1:250–1:500), (50 mL) Antibody CD124-BB700 (rat, monoclonal) BD 742172, RRID: AB_2871410 (1:250–1:500), (50 mL) Antibody CD122-APC (rat, monoclonal) BD 564924, RRID: AB_2739009 (1:250–1:500), (50 mL) Antibody CD132-BV421 (rat, monoclonal) BD 740039, RRID: AB_2739809 (1:250–1:500), (50 mL) Antibody CD213a1-Biotin (rabbit, polyclonal) MyBioSource MBS2001753 (1:250–1:500), (50 mL) Antibody pSTAT3-AF647 (mouse, monoclonal) BD 557815, RRID: AB_647144 (1:100–1:200), (25 mL) Antibody pSTAT5-AF647 (mouse, monoclonal) BD 612599, RRID: AB_399882 (1:100–1:200), (25 mL) Antibody pSTAT6-AF647 (mouse, monoclonal) BD 558242, RRID: AB_647145 (1:100–1:200), (25 mL) Antibody IL-10R biotin (rat, monoclonal) Biolegend 112704, RRID: AB_313517 (1:250–1:500), (50 mL) Antibody SA-APCefluor780 eBioscience 47-4317-82, RRID: AB_ 10366688 (1:250–1:500), (50 mL) Antibody SA-BV605 BD 563260, RRID: AB_2869476 (1:250–1:500), (50 mL) Peptide, recombinant protein Recombinant IL-2 R and D Systems 402 ML-020 Peptide, recombinant protein Recombinant IL-4 R and D Systems 404 ML-010 Peptide, recombinant protein Recombinant IL-10 R and D Systems 417 ML Commercial assay, kit IFNg ELISA MAX Biolegend 430803 Commercial assay, kit IL-4 ELISA MAX Biolegend 431102 Commercial assay, kit IL-10 ELISA MAX Biolegend 431413 Commercial assay, kit IL-17A ELISA MAX Biolegend 432501 Commercial assay, kit MOG35-55/CFA Emulsion PTX Hooke Laboratories EK-2110 Chemical compound, drug STAT5i Stemcell 73852 Continued on next page Zhou et al. eLife 2021;10:e57417. .. DOI: https://doi.org/10.7554/eLife.57417 19 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug House dust mite Greer XPB8103A2.5 Chemical compound, drug Ovalbumin Sigma-Aldrich A5503 Chemical compound, drug Cockroach antigen Greer XPB46D3A4 Chemical compound, drug Methacholine Sigma-Aldrich 62-51-1 Software, algorithm Prism Graphpad Versions 5 and 7, RRID: SCR_002798 Software, algorithm FlowJo FlowJo v10.7, RRID:SCR_008520 Other FACS-ARIA SORP BD Other Attune NxT Thermo Fisher Other Sytox Red Invitrogen S34859 Other Fixable Viability Stain 510 BD 564406 Other MOG I-A(b) tetramer-BV421 NIH Tetramer Core GWYRSPFSRVVH Other Control I-A(b) tetramer-BV421 NIH Tetramer Core PVSKMRMATPLLMQA Other CellTrace Violet Invitrogen C34557 or C34571 Other Europium-conjugated streptavidin Perkin-Elmer 1244–360 Other DELFIA Enhancement solution Perkin-Elmer 4001–0010 Other 10% formalin Anachemia 41916–700 Other Hematoxylin Ricca 3536–32 Other Eosin EMD Harleco 588X-75 Other Solvent Blue 38 Sigma-Aldrich S3382 Other Percoll GE 17-0891-02 .. C57BL/6J (Stock #000664), Il10GFP (B6.129S6-Il10tm1Flv/RthsnJ, Stock #008379), Foxp3RFP (C57BL/6Foxp3tm1Flv/J, Stock #008374), Foxp3creYFP (B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J, Stock 016959), and Il10-/- (B6.129P2-Il10tm1Cgn/J, Stock #002251) mice, all on the C57BL/6 background, as well as Foxp3GFP mice on the BALB/c background (C.Cg-Foxp3tm2Tch, Stock #006769) were purchased from the Jackson Laboratory (Bar Harbor, ME).

    Staining:

    Article Title: Integration of IL-2 and IL-4 signals coordinates divergent regulatory T cell responses and drives therapeutic efficacy
    Article Snippet: To obtain, contact the Cobb lab. Antibody aCD4-magnetic beads (rat, monoclonal) Miltenyi Biotec 130-049-201, RRID:AB_ 2722753 (1:50–1:100), (50 mL) Antibody aCD3 (Armenian hamster, monoclonal) eBioscience 16-0031-85, RRID: AB_ 468848 (1:400), (50 mL) Antibody aCD28 (Syrian hamster, monoclonal) eBioscience 16-0281-82, RRID: AB_ 468921 (1:400), (50 mL) Antibody CD4-PE (rat, monoclonal) eBioscience 12-0042-82, RRID: AB_ 465510 (1:250–1:500), (50 mL) Antibody CD4-AF647 (rat, monoclonal) Biolegend 100530, RRID: AB_389325 (1:250–1:500), (50 mL) Antibody CD4-BV421 (rat, monoclonal) BD 740007, RRID: AB_2739779 (1:250–1:500), (50 mL) Antibody F4/80-BV711 (rat, monoclonal) BD 565612, RRID: AB_2734769 (1:250–1:500), (50 mL) Antibody CD25-PECy7 (rat, monoclonal) BD 561780, RRID: AB_ 10893596 (1:250–1:500), (50 mL) Antibody CD25-PE (rat, monoclonal) eBioscience 12-0251-82, RRID: AB_ 465607 (1:250–1:500), (50 mL) Antibody CD124-BB700 (rat, monoclonal) BD 742172, RRID: AB_2871410 (1:250–1:500), (50 mL) Antibody CD122-APC (rat, monoclonal) BD 564924, RRID: AB_2739009 (1:250–1:500), (50 mL) Antibody CD132-BV421 (rat, monoclonal) BD 740039, RRID: AB_2739809 (1:250–1:500), (50 mL) Antibody CD213a1-Biotin (rabbit, polyclonal) MyBioSource MBS2001753 (1:250–1:500), (50 mL) Antibody pSTAT3-AF647 (mouse, monoclonal) BD 557815, RRID: AB_647144 (1:100–1:200), (25 mL) Antibody pSTAT5-AF647 (mouse, monoclonal) BD 612599, RRID: AB_399882 (1:100–1:200), (25 mL) Antibody pSTAT6-AF647 (mouse, monoclonal) BD 558242, RRID: AB_647145 (1:100–1:200), (25 mL) Antibody IL-10R biotin (rat, monoclonal) Biolegend 112704, RRID: AB_313517 (1:250–1:500), (50 mL) Antibody SA-APCefluor780 eBioscience 47-4317-82, RRID: AB_ 10366688 (1:250–1:500), (50 mL) Antibody SA-BV605 BD 563260, RRID: AB_2869476 (1:250–1:500), (50 mL) Peptide, recombinant protein Recombinant IL-2 R and D Systems 402 ML-020 Peptide, recombinant protein Recombinant IL-4 R and D Systems 404 ML-010 Peptide, recombinant protein Recombinant IL-10 R and D Systems 417 ML Commercial assay, kit IFNg ELISA MAX Biolegend 430803 Commercial assay, kit IL-4 ELISA MAX Biolegend 431102 Commercial assay, kit IL-10 ELISA MAX Biolegend 431413 Commercial assay, kit IL-17A ELISA MAX Biolegend 432501 Commercial assay, kit MOG35-55/CFA Emulsion PTX Hooke Laboratories EK-2110 Chemical compound, drug STAT5i Stemcell 73852 Continued on next page Zhou et al. eLife 2021;10:e57417. .. DOI: https://doi.org/10.7554/eLife.57417 19 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug House dust mite Greer XPB8103A2.5 Chemical compound, drug Ovalbumin Sigma-Aldrich A5503 Chemical compound, drug Cockroach antigen Greer XPB46D3A4 Chemical compound, drug Methacholine Sigma-Aldrich 62-51-1 Software, algorithm Prism Graphpad Versions 5 and 7, RRID: SCR_002798 Software, algorithm FlowJo FlowJo v10.7, RRID:SCR_008520 Other FACS-ARIA SORP BD Other Attune NxT Thermo Fisher Other Sytox Red Invitrogen S34859 Other Fixable Viability Stain 510 BD 564406 Other MOG I-A(b) tetramer-BV421 NIH Tetramer Core GWYRSPFSRVVH Other Control I-A(b) tetramer-BV421 NIH Tetramer Core PVSKMRMATPLLMQA Other CellTrace Violet Invitrogen C34557 or C34571 Other Europium-conjugated streptavidin Perkin-Elmer 1244–360 Other DELFIA Enhancement solution Perkin-Elmer 4001–0010 Other 10% formalin Anachemia 41916–700 Other Hematoxylin Ricca 3536–32 Other Eosin EMD Harleco 588X-75 Other Solvent Blue 38 Sigma-Aldrich S3382 Other Percoll GE 17-0891-02 .. C57BL/6J (Stock #000664), Il10GFP (B6.129S6-Il10tm1Flv/RthsnJ, Stock #008379), Foxp3RFP (C57BL/6Foxp3tm1Flv/J, Stock #008374), Foxp3creYFP (B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J, Stock 016959), and Il10-/- (B6.129P2-Il10tm1Cgn/J, Stock #002251) mice, all on the C57BL/6 background, as well as Foxp3GFP mice on the BALB/c background (C.Cg-Foxp3tm2Tch, Stock #006769) were purchased from the Jackson Laboratory (Bar Harbor, ME).

    Control:

    Article Title: Integration of IL-2 and IL-4 signals coordinates divergent regulatory T cell responses and drives therapeutic efficacy
    Article Snippet: To obtain, contact the Cobb lab. Antibody aCD4-magnetic beads (rat, monoclonal) Miltenyi Biotec 130-049-201, RRID:AB_ 2722753 (1:50–1:100), (50 mL) Antibody aCD3 (Armenian hamster, monoclonal) eBioscience 16-0031-85, RRID: AB_ 468848 (1:400), (50 mL) Antibody aCD28 (Syrian hamster, monoclonal) eBioscience 16-0281-82, RRID: AB_ 468921 (1:400), (50 mL) Antibody CD4-PE (rat, monoclonal) eBioscience 12-0042-82, RRID: AB_ 465510 (1:250–1:500), (50 mL) Antibody CD4-AF647 (rat, monoclonal) Biolegend 100530, RRID: AB_389325 (1:250–1:500), (50 mL) Antibody CD4-BV421 (rat, monoclonal) BD 740007, RRID: AB_2739779 (1:250–1:500), (50 mL) Antibody F4/80-BV711 (rat, monoclonal) BD 565612, RRID: AB_2734769 (1:250–1:500), (50 mL) Antibody CD25-PECy7 (rat, monoclonal) BD 561780, RRID: AB_ 10893596 (1:250–1:500), (50 mL) Antibody CD25-PE (rat, monoclonal) eBioscience 12-0251-82, RRID: AB_ 465607 (1:250–1:500), (50 mL) Antibody CD124-BB700 (rat, monoclonal) BD 742172, RRID: AB_2871410 (1:250–1:500), (50 mL) Antibody CD122-APC (rat, monoclonal) BD 564924, RRID: AB_2739009 (1:250–1:500), (50 mL) Antibody CD132-BV421 (rat, monoclonal) BD 740039, RRID: AB_2739809 (1:250–1:500), (50 mL) Antibody CD213a1-Biotin (rabbit, polyclonal) MyBioSource MBS2001753 (1:250–1:500), (50 mL) Antibody pSTAT3-AF647 (mouse, monoclonal) BD 557815, RRID: AB_647144 (1:100–1:200), (25 mL) Antibody pSTAT5-AF647 (mouse, monoclonal) BD 612599, RRID: AB_399882 (1:100–1:200), (25 mL) Antibody pSTAT6-AF647 (mouse, monoclonal) BD 558242, RRID: AB_647145 (1:100–1:200), (25 mL) Antibody IL-10R biotin (rat, monoclonal) Biolegend 112704, RRID: AB_313517 (1:250–1:500), (50 mL) Antibody SA-APCefluor780 eBioscience 47-4317-82, RRID: AB_ 10366688 (1:250–1:500), (50 mL) Antibody SA-BV605 BD 563260, RRID: AB_2869476 (1:250–1:500), (50 mL) Peptide, recombinant protein Recombinant IL-2 R and D Systems 402 ML-020 Peptide, recombinant protein Recombinant IL-4 R and D Systems 404 ML-010 Peptide, recombinant protein Recombinant IL-10 R and D Systems 417 ML Commercial assay, kit IFNg ELISA MAX Biolegend 430803 Commercial assay, kit IL-4 ELISA MAX Biolegend 431102 Commercial assay, kit IL-10 ELISA MAX Biolegend 431413 Commercial assay, kit IL-17A ELISA MAX Biolegend 432501 Commercial assay, kit MOG35-55/CFA Emulsion PTX Hooke Laboratories EK-2110 Chemical compound, drug STAT5i Stemcell 73852 Continued on next page Zhou et al. eLife 2021;10:e57417. .. DOI: https://doi.org/10.7554/eLife.57417 19 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug House dust mite Greer XPB8103A2.5 Chemical compound, drug Ovalbumin Sigma-Aldrich A5503 Chemical compound, drug Cockroach antigen Greer XPB46D3A4 Chemical compound, drug Methacholine Sigma-Aldrich 62-51-1 Software, algorithm Prism Graphpad Versions 5 and 7, RRID: SCR_002798 Software, algorithm FlowJo FlowJo v10.7, RRID:SCR_008520 Other FACS-ARIA SORP BD Other Attune NxT Thermo Fisher Other Sytox Red Invitrogen S34859 Other Fixable Viability Stain 510 BD 564406 Other MOG I-A(b) tetramer-BV421 NIH Tetramer Core GWYRSPFSRVVH Other Control I-A(b) tetramer-BV421 NIH Tetramer Core PVSKMRMATPLLMQA Other CellTrace Violet Invitrogen C34557 or C34571 Other Europium-conjugated streptavidin Perkin-Elmer 1244–360 Other DELFIA Enhancement solution Perkin-Elmer 4001–0010 Other 10% formalin Anachemia 41916–700 Other Hematoxylin Ricca 3536–32 Other Eosin EMD Harleco 588X-75 Other Solvent Blue 38 Sigma-Aldrich S3382 Other Percoll GE 17-0891-02 .. C57BL/6J (Stock #000664), Il10GFP (B6.129S6-Il10tm1Flv/RthsnJ, Stock #008379), Foxp3RFP (C57BL/6Foxp3tm1Flv/J, Stock #008374), Foxp3creYFP (B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J, Stock 016959), and Il10-/- (B6.129P2-Il10tm1Cgn/J, Stock #002251) mice, all on the C57BL/6 background, as well as Foxp3GFP mice on the BALB/c background (C.Cg-Foxp3tm2Tch, Stock #006769) were purchased from the Jackson Laboratory (Bar Harbor, ME).

    Solvent:

    Article Title: Integration of IL-2 and IL-4 signals coordinates divergent regulatory T cell responses and drives therapeutic efficacy
    Article Snippet: To obtain, contact the Cobb lab. Antibody aCD4-magnetic beads (rat, monoclonal) Miltenyi Biotec 130-049-201, RRID:AB_ 2722753 (1:50–1:100), (50 mL) Antibody aCD3 (Armenian hamster, monoclonal) eBioscience 16-0031-85, RRID: AB_ 468848 (1:400), (50 mL) Antibody aCD28 (Syrian hamster, monoclonal) eBioscience 16-0281-82, RRID: AB_ 468921 (1:400), (50 mL) Antibody CD4-PE (rat, monoclonal) eBioscience 12-0042-82, RRID: AB_ 465510 (1:250–1:500), (50 mL) Antibody CD4-AF647 (rat, monoclonal) Biolegend 100530, RRID: AB_389325 (1:250–1:500), (50 mL) Antibody CD4-BV421 (rat, monoclonal) BD 740007, RRID: AB_2739779 (1:250–1:500), (50 mL) Antibody F4/80-BV711 (rat, monoclonal) BD 565612, RRID: AB_2734769 (1:250–1:500), (50 mL) Antibody CD25-PECy7 (rat, monoclonal) BD 561780, RRID: AB_ 10893596 (1:250–1:500), (50 mL) Antibody CD25-PE (rat, monoclonal) eBioscience 12-0251-82, RRID: AB_ 465607 (1:250–1:500), (50 mL) Antibody CD124-BB700 (rat, monoclonal) BD 742172, RRID: AB_2871410 (1:250–1:500), (50 mL) Antibody CD122-APC (rat, monoclonal) BD 564924, RRID: AB_2739009 (1:250–1:500), (50 mL) Antibody CD132-BV421 (rat, monoclonal) BD 740039, RRID: AB_2739809 (1:250–1:500), (50 mL) Antibody CD213a1-Biotin (rabbit, polyclonal) MyBioSource MBS2001753 (1:250–1:500), (50 mL) Antibody pSTAT3-AF647 (mouse, monoclonal) BD 557815, RRID: AB_647144 (1:100–1:200), (25 mL) Antibody pSTAT5-AF647 (mouse, monoclonal) BD 612599, RRID: AB_399882 (1:100–1:200), (25 mL) Antibody pSTAT6-AF647 (mouse, monoclonal) BD 558242, RRID: AB_647145 (1:100–1:200), (25 mL) Antibody IL-10R biotin (rat, monoclonal) Biolegend 112704, RRID: AB_313517 (1:250–1:500), (50 mL) Antibody SA-APCefluor780 eBioscience 47-4317-82, RRID: AB_ 10366688 (1:250–1:500), (50 mL) Antibody SA-BV605 BD 563260, RRID: AB_2869476 (1:250–1:500), (50 mL) Peptide, recombinant protein Recombinant IL-2 R and D Systems 402 ML-020 Peptide, recombinant protein Recombinant IL-4 R and D Systems 404 ML-010 Peptide, recombinant protein Recombinant IL-10 R and D Systems 417 ML Commercial assay, kit IFNg ELISA MAX Biolegend 430803 Commercial assay, kit IL-4 ELISA MAX Biolegend 431102 Commercial assay, kit IL-10 ELISA MAX Biolegend 431413 Commercial assay, kit IL-17A ELISA MAX Biolegend 432501 Commercial assay, kit MOG35-55/CFA Emulsion PTX Hooke Laboratories EK-2110 Chemical compound, drug STAT5i Stemcell 73852 Continued on next page Zhou et al. eLife 2021;10:e57417. .. DOI: https://doi.org/10.7554/eLife.57417 19 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug House dust mite Greer XPB8103A2.5 Chemical compound, drug Ovalbumin Sigma-Aldrich A5503 Chemical compound, drug Cockroach antigen Greer XPB46D3A4 Chemical compound, drug Methacholine Sigma-Aldrich 62-51-1 Software, algorithm Prism Graphpad Versions 5 and 7, RRID: SCR_002798 Software, algorithm FlowJo FlowJo v10.7, RRID:SCR_008520 Other FACS-ARIA SORP BD Other Attune NxT Thermo Fisher Other Sytox Red Invitrogen S34859 Other Fixable Viability Stain 510 BD 564406 Other MOG I-A(b) tetramer-BV421 NIH Tetramer Core GWYRSPFSRVVH Other Control I-A(b) tetramer-BV421 NIH Tetramer Core PVSKMRMATPLLMQA Other CellTrace Violet Invitrogen C34557 or C34571 Other Europium-conjugated streptavidin Perkin-Elmer 1244–360 Other DELFIA Enhancement solution Perkin-Elmer 4001–0010 Other 10% formalin Anachemia 41916–700 Other Hematoxylin Ricca 3536–32 Other Eosin EMD Harleco 588X-75 Other Solvent Blue 38 Sigma-Aldrich S3382 Other Percoll GE 17-0891-02 .. C57BL/6J (Stock #000664), Il10GFP (B6.129S6-Il10tm1Flv/RthsnJ, Stock #008379), Foxp3RFP (C57BL/6Foxp3tm1Flv/J, Stock #008374), Foxp3creYFP (B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J, Stock 016959), and Il10-/- (B6.129P2-Il10tm1Cgn/J, Stock #002251) mice, all on the C57BL/6 background, as well as Foxp3GFP mice on the BALB/c background (C.Cg-Foxp3tm2Tch, Stock #006769) were purchased from the Jackson Laboratory (Bar Harbor, ME).



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