smartscribetm reverse transcriptase clontech (TaKaRa)
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smartscribetm reverse transcriptase clontech
Smartscribetm Reverse Transcriptase Clontech, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smartscribetm+reverse+transcriptase+clontech/SMARTer+Universal+Low+Input+RNA+Kit+for+Sequencing/pm40769155-298-131-134
Average 96 stars, based on 295 article reviews
Smartscribetm Reverse Transcriptase Clontech, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smartscribetm+reverse+transcriptase+clontech/SMARTer+Universal+Low+Input+RNA+Kit+for+Sequencing/pm40769155-298-131-134
Average 96 stars, based on 295 article reviews
smartscribetm reverse transcriptase clontech - by Bioz Stars,
2026-11
96/100 stars
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Single Cell:Article Title: Systematic evaluation of single-cell multimodal data integration enhances cell type resolution and discovery of clinically relevant states in complex tissues Article Snippet: We pooled individual libraries with equal volumes and sequenced on a NextSeq 500 flow cell (Illumina) with 50 bases for read 1, 25 bases for read 2, and 8 bases each for index 1 and index 2, and aimed for about 1 million reads per cell. .. For one (lib_34) of those four samples, full-length single-cell RNA-seq libraries were also prepared separately using the Article Title: Systematic evaluation of single-cell multimodal data integration enhances cell type resolution and discovery of clinically relevant states in complex tissues. Article Snippet: We pooled individual libraries with equal volumes and sequenced on a NextSeq 500 flow cell (Illumina) with 50 bases for read 1, 25 bases for read 2, and 8 bases each for index 1 and index 2, and aimed for about 1 million reads per cell. .. For one (lib_34) of those four samples, full-length single-cell RNA-seq libraries were also prepared separately using the RNA Sequencing:Article Title: Systematic evaluation of single-cell multimodal data integration enhances cell type resolution and discovery of clinically relevant states in complex tissues Article Snippet: We pooled individual libraries with equal volumes and sequenced on a NextSeq 500 flow cell (Illumina) with 50 bases for read 1, 25 bases for read 2, and 8 bases each for index 1 and index 2, and aimed for about 1 million reads per cell. .. For one (lib_34) of those four samples, full-length single-cell RNA-seq libraries were also prepared separately using the Article Title: Systematic evaluation of single-cell multimodal data integration enhances cell type resolution and discovery of clinically relevant states in complex tissues. Article Snippet: We pooled individual libraries with equal volumes and sequenced on a NextSeq 500 flow cell (Illumina) with 50 bases for read 1, 25 bases for read 2, and 8 bases each for index 1 and index 2, and aimed for about 1 million reads per cell. .. For one (lib_34) of those four samples, full-length single-cell RNA-seq libraries were also prepared separately using the Sequencing:Article Title: Systematic evaluation of single-cell multimodal data integration enhances cell type resolution and discovery of clinically relevant states in complex tissues Article Snippet: We pooled individual libraries with equal volumes and sequenced on a NextSeq 500 flow cell (Illumina) with 50 bases for read 1, 25 bases for read 2, and 8 bases each for index 1 and index 2, and aimed for about 1 million reads per cell. .. For one (lib_34) of those four samples, full-length single-cell RNA-seq libraries were also prepared separately using the Article Title: Systematic evaluation of single-cell multimodal data integration enhances cell type resolution and discovery of clinically relevant states in complex tissues. Article Snippet: We pooled individual libraries with equal volumes and sequenced on a NextSeq 500 flow cell (Illumina) with 50 bases for read 1, 25 bases for read 2, and 8 bases each for index 1 and index 2, and aimed for about 1 million reads per cell. .. For one (lib_34) of those four samples, full-length single-cell RNA-seq libraries were also prepared separately using the Article Title: Combined Patch-clamp Electrophysiology and Single-Cell Genomic Analysis Reveal Spiking Tumor Cells at the Neocortical Glioblastoma Interface in Humans. Article Snippet: .. 190 cDNA amplification, library construction, and sequencing 191 Following nucleus collection, reverse transcription and cDNA amplification were performed 192 using the Article Title: Identification of host lncRNAs that impact Venezuelan equine encephalitis virus TC-83 replication. Article Snippet: Ribosomal RNA was depleted using the RiboGone Kit (Takara Bio, Cat. No. 634846) according to the manufacturer’s instructions. .. The RNA was prepared for sequencing using the SMARTer Article Title: Microglial HDAC3 inhibits the migration of CD8-positive T cell in the development of EAE by restraining the expression of CCL5. Article Snippet: Then, primary microglia were purified with anti-CD11b microbeads (#130-093-727, Miltenyi) and the RNA was extracted with Trizol reagent. .. The mRNA was furtherly purified as above, and the cDNA libraries were generated by using a SMARTer® other:Article Title: Site-Specific Entry Factors Define Cellular Susceptibility to SARS-CoV-2 in Human Tissues Article Snippet: Cellular suspensions were later stained with Live/Dead Aqua (Invitrogen), followed by surface staining with the antibodies required for phenotype identification. Adapter Ligation:Article Title: Apelin-13 alleviates post-infarction injury by modulating macrophage inflammation via APJ-dependent inhibition of NLRP3-mediated pyroptosis. Article Snippet: Background: Sustained macrophage-driven inflammation critically exacerbates post-infarction myocardial injury and remodeling.. While Apelin-13 (A13) is known for its cardiovascular benefits, its direct immunomodulatory role on macrophages after myocardial infarction (MI) remains undefined.. Methods: We established a mouse MI model and a hypoxia/hypoglycemia (Hyp) model with macrophagecardiomyocyte Transwell co-culture. Polymerase Chain Reaction:Article Title: Apelin-13 alleviates post-infarction injury by modulating macrophage inflammation via APJ-dependent inhibition of NLRP3-mediated pyroptosis. Article Snippet: Background: Sustained macrophage-driven inflammation critically exacerbates post-infarction myocardial injury and remodeling.. While Apelin-13 (A13) is known for its cardiovascular benefits, its direct immunomodulatory role on macrophages after myocardial infarction (MI) remains undefined.. Methods: We established a mouse MI model and a hypoxia/hypoglycemia (Hyp) model with macrophagecardiomyocyte Transwell co-culture. Amplification:Article Title: Apelin-13 alleviates post-infarction injury by modulating macrophage inflammation via APJ-dependent inhibition of NLRP3-mediated pyroptosis. Article Snippet: Background: Sustained macrophage-driven inflammation critically exacerbates post-infarction myocardial injury and remodeling.. While Apelin-13 (A13) is known for its cardiovascular benefits, its direct immunomodulatory role on macrophages after myocardial infarction (MI) remains undefined.. Methods: We established a mouse MI model and a hypoxia/hypoglycemia (Hyp) model with macrophagecardiomyocyte Transwell co-culture. Article Title: Combined Patch-clamp Electrophysiology and Single-Cell Genomic Analysis Reveal Spiking Tumor Cells at the Neocortical Glioblastoma Interface in Humans. Article Snippet: .. 190 cDNA amplification, library construction, and sequencing 191 Following nucleus collection, reverse transcription and cDNA amplification were performed 192 using the Reverse Transcription:Article Title: Combined Patch-clamp Electrophysiology and Single-Cell Genomic Analysis Reveal Spiking Tumor Cells at the Neocortical Glioblastoma Interface in Humans. Article Snippet: .. 190 cDNA amplification, library construction, and sequencing 191 Following nucleus collection, reverse transcription and cDNA amplification were performed 192 using the Generated:Article Title: “Chronic morphine treatment induces a conserved Smchd1-dependent epigenetic memory that disrupts X-chromosome inactivation and genomic imprinting” Article Snippet: RNA concentration was measured by absorbance at 260 nm, and purity was verified by calculating the 260/280 nm ratio. cDNA synthesis was reverse transcribed using the iScript cDNA Synthesis Kit (1708891, Bio-Rad) for mESCs, EpiLCs and hiPSCs samples. .. For blastocyst-derived RNA, cDNA was generated with the SMARTer Article Title: Microglial HDAC3 inhibits the migration of CD8-positive T cell in the development of EAE by restraining the expression of CCL5. Article Snippet: Then, primary microglia were purified with anti-CD11b microbeads (#130-093-727, Miltenyi) and the RNA was extracted with Trizol reagent. .. The mRNA was furtherly purified as above, and the cDNA libraries were generated by using a SMARTer® Purification:Article Title: Microglial HDAC3 inhibits the migration of CD8-positive T cell in the development of EAE by restraining the expression of CCL5. Article Snippet: Then, primary microglia were purified with anti-CD11b microbeads (#130-093-727, Miltenyi) and the RNA was extracted with Trizol reagent. .. The mRNA was furtherly purified as above, and the cDNA libraries were generated by using a SMARTer® |