smartscribe reverse transcriptase (Westburg bv)

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Westburg bv smartscribe reverse transcriptase
Smartscribe Reverse Transcriptase, supplied by Westburg bv, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smartscribe+reverse+transcriptase/smartscribe+reverse+transcriptase/bio_rxiv__303834-182-15-18
Average 90 stars, based on 1 article reviews

smartscribe reverse transcriptase - by Bioz Stars, 2026-11

90/100 stars

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Lysis:

Article Title: TGF-β induced CXCL13 in CD8+ T cells is associated with tertiary lymphoid structures in cancer
Article Snippet: .. After lysis of the cells, the transcriptomes were amplified by a modified SMART-Seq2 protocol using SmartScribe reverse transcriptase (Westburg-Clontech, CL639537), based on a previously published protocol (Picelli et al. REF ). .. Sequencing libraries were prepared using the Illumina Nextera XT DNA sample preparation kit.

Amplification:

Article Title: A Transcriptionally Distinct CXCL13+CD103+CD8+ T-cell Population Is Associated with B-cell Recruitment and Neoantigen Load in Human Cancer
Article Snippet: .. The transcriptomes were amplified by a modified SMART-Seq2 protocol using SmartScribe reverse transcriptase (Westburg-Clontech, CL639537), based on a previously published protocol [30]. ..

Modification:

Reverse Transcription:

Article Title: Transcriptional Activity and Stability of CD39+CD103+CD8+ T Cells in Human High-Grade Endometrial Cancer
Article Snippet: .. In brief, SmartScribe reverse transcriptase (Westburg-Clontech) and a template switching oligo (BC-TSO) were used to generate cDNA. .. Next, a PCR preamplification step was done with KAPA HiFi HotStart Ready Mix (Roche Diagnostics) and a custom-made PCR primer.

Article Title: Expression of CD39 Identifies Activated Intratumoral CD8+ T Cells in Mismatch Repair Deficient Endometrial Cancer
Article Snippet: .. In brief, SmartScribe reverse transcriptase (Westburg-Clontech, London, UK) and a template-switching oligo (BC-TSO), were used to generate cDNA. ..

Article Title: CD20 positive CD8 T cells are a unique and transcriptionally-distinct subset of T cells with distinct transmigration properties
Article Snippet: .. In brief, SmartScribe reverse transcriptase (Westburg-Clontech) and a template switching oligo (BC-TSO) were used to generate cDNA. .. Next, a PCR preamplification step was done with KAPA HiFi HotStart Ready Mix (Roche Diagnostics) and a custom-made PCR primer.

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