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CapitalBio Corporation smartarrayertm 136 printer
Smartarrayertm 136 Printer, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smartarrayertm+136/smartarraytm/pmc05915573-243-18-21
Average 90 stars, based on 1 article reviews
smartarrayertm 136 printer - by Bioz Stars, 2026-09
90/100 stars

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Article Title: A fast sample processing strategy for large-scale profiling of human urine phosphoproteome by mass spectrometry.
Article Snippet: Liquid biopsies using body fluids have gained much attention in recent years due to their multiple advantages in clinical diagnosis, such as less/non-invasive collection, suitability for longitudinal disease monitoring, and better representation of tumor heterogeneity.. As an attractive choice for liquid biopsy, urine proteins and their posttranslational modifications (PTMs) have the potential to offer significant insights into physiological variations and pathological changes in the human body.. However, due to the intrinsically large variability of urine proteins and their PTMs among different individuals, there is a high demand for strategies for high-throughput analysis of a large number of samples to obtain a comprehensive view and a reliable reference interval of the urine proteome.

Microarray:

Article Title: Development of multiple genome-wide proteome microarrays comprised wafer substrate-based chip and its scanner: An advanced high-throughput and sensitivity for molecular interactions studies.
Article Snippet: Proteome microarray technology enables high-throughput analysis of protein interactions with all kinds of molecules.. Wafer (6-inch) substrates offer a promising alternative to conventional glass (2.6 × 7.6 cm) substrates for carrying proteomes.. This study aims to develop high-density wafer-based proteome microarrays and a corresponding fluorescence scanner.

Article Title: Efficacy analysis of combining three comparative-omics profilings to screen candidate biological macromolecules.
Article Snippet: To evaluate the efficacy of integrated comparative omics in screening candidate biological macromolecules, three methods, namely, 2-DE ?. MALDI-TOF-MS, tumor-associated cDNA microarray and whole-transcriptome cDNA microarray were examined by three biotechnological companies to compare a stable transfected cell line with its control one.. The results showed that the percentages of the up-regulate in three methods are largely consistent.

Labeling:

Article Title: Cancer stem-like cells enriched with CD29 and CD44 markers exhibit molecular characteristics with epithelial-mesenchymal transition in squamous cell carcinoma.
Article Snippet: Increasing evidences have indicated that only a phenotypic subset of cancer cells, termed as the cancer stem cells (CSCs), is capable of initiating tumor growth and provide a reservoir of cells that cause tumor recurrence after therapy.. Epithelial–mesenchymal transition (EMT), a cell type change from an epithelial cobblestone phenotype to an elongated fibroblastic phenotype, plays a critical role not only in tumor metastasis but also in tumor recurrence and contributes to drug resistance.. Accumulating evidence has shown that cells with an EMT phenotype are rich sources for CSCs, suggesting a biological link between EMT and CSCs; thus study on the link will help understand the cellular and molecular mechanisms of tumor metastasis and drug resistance.

Article Title: A serum microRNA signature predicts trastuzumab benefit in HER2-positive metastatic breast cancer patients
Article Snippet: .. Briefly, the probes (40 μM final concentration) mixed with printing buffer were printed onto slides in duplicate using SmartArrayerTM 136 printer (CapitalBio Inc, Beijing, China); RNA extracted from 600 μl serum was dephosphorylated and labeled with 100 nmol l −1 of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 μl at 16 °C overnight. .. The labeled RNAs were mixed and hybridized to the array with a 2× hybridization solution (final concentration: 5× Denhart’s solution, 0.5% sodium dodecyl sulfate (SDS), 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46 °C for 12–16 h. After washing, slides were scanned with a LuxScan 10 K Microarray Scanner (Capital Bio, Beijing, China), and images were analyzed with th GenePix Pro 6.0 software (Axon Instruments, Foster City, CA, USA).

Concentration Assay:

Article Title: A serum microRNA signature predicts trastuzumab benefit in HER2-positive metastatic breast cancer patients
Article Snippet: .. Briefly, the probes (40 μM final concentration) mixed with printing buffer were printed onto slides in duplicate using SmartArrayerTM 136 printer (CapitalBio Inc, Beijing, China); RNA extracted from 600 μl serum was dephosphorylated and labeled with 100 nmol l −1 of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 μl at 16 °C overnight. .. The labeled RNAs were mixed and hybridized to the array with a 2× hybridization solution (final concentration: 5× Denhart’s solution, 0.5% sodium dodecyl sulfate (SDS), 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46 °C for 12–16 h. After washing, slides were scanned with a LuxScan 10 K Microarray Scanner (Capital Bio, Beijing, China), and images were analyzed with th GenePix Pro 6.0 software (Axon Instruments, Foster City, CA, USA).



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