smart seq library prep kit (TaKaRa)

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TaKaRa smart seq library prep kit
Smart Seq Library Prep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smart+seq+library+prep+kit/Unique+Dual+Index+Kit+-+96U/us12629681-231-1-10
Average 94 stars, based on 16 article reviews

smart seq library prep kit - by Bioz Stars, 2026-11

94/100 stars

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Article Title: Cellular deconstruction of the human skeletal muscle microenvironment identifies an exercise-induced histaminergic crosstalk.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 4-imidazole acetic acid hydrochloride Merck Cat#219991 L-histidine Merck Cat#H8000 histamine-a,a,b,b-d4 dihydrochloride Merck Cat#762962 N-methylhistamine Sanbio Cat#19516-10 1-methyl-4-imidazole aceKc acid hydrochloride Sanbio Cat#18815-10 Critical commercial assays Pierce protein assay kit Thermo Fisher Scientific Cat#23225 Pierce Reversible Protein Stain Kit for PVDF membranes Thermo Fisher Scientific Cat#24585 Immobilon Forte Western HRP substrate Millipore Cat#WBLUF0020 RNAscope multiplex fluorescent reagent kit v2 Advanced Cell Diagnostics Cat#323285 SingleShot Cell Lysis kit Bio-Rad Cat#1725080 QuantSeq-Pool 3’ mRNA-Seq library prep kit Lexogen Cat#139.96 High sensitivity small DNA Fragment Analysis kit Agilent Technologies Cat#DNF-477-0500 NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles) Illumina Cat#20028315 NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles) Illumina Cat#20028316 miRNeasy Mini kit Qiagen Cat#217004 HL-dsDNase kit ArcticZymes Cat#70800 SMARTer Stranded Total RNA-Seq Kit (v3 - Pico Input 891 Mammalian) Takara Bio Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio Cat#634452 SMARTer RNA Unique Dual Index Kit – 96U Set B Takara Bio Cat#634457 Deposited data Bulk muscle RNA-seq data with single histamine receptor blockade This paper EGAD50000000612 Single-fiber RNA-seq data This paper EGAD50000000612 Single-cell RNA-seq data Turiel et al.22 GSE235143 Bulk muscle RNA-seq data with double histamine receptor blockade Romero et al.60 GSE71972 Data S1 – Source data This paper N/A Software and algorithms R v. 4.2.3 CRAN https://www.r-project.org QuPath v. 0.5.1 QuPath https://qupath.github.io ImageLab v. 6.1 Bio-Rad https://www.bio-rad.com/en-be/product/ image-lab-software?ID=KRE6P5E8Z MassLynx v. 4.2 Waters https://www.waters.com/nextgen/en/ products/informatics-and-software/massspectrometry-software/masslynx-massspectrometry-software.html?srsltid= AfmBOoo1MHb0IwAWzdQXWenTttxdBey79cVBf-UtKPRTbl66nN7OlBw QuantSeq Pool analysis pipeline Lexogen https://github.com/Lexogen-Tools/ quantseqpool_analysis Illustrator v. 29.1 Adobe https://www.adobe.com/be_en/ products/illustrator.html Other Cyclus 2 ergometer RBM Elektronik-automation https://www.cyclus2.com/en/ Excalibur Sport ergometer Lode https://lode-ergometry.com/product/ excalibur-sport/ Fexofenadine Telfast N/A Famotidine Aurobindo Pharma N/A Microdialysis catheter (20 kDa) MDialysis AB #8010514 Cell Metabolism 37, 1–15.e1–e7, April 1, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 4-imidazole acetic acid hydrochloride Merck Cat#219991 L-histidine Merck Cat#H8000 histamine-a,a,b,b-d4 dihydrochloride Merck Cat#762962 N-methylhistamine Sanbio Cat#19516-10 1-methyl-4-imidazole aceKc acid hydrochloride Sanbio Cat#18815-10 Critical commercial assays Pierce protein assay kit Thermo Fisher Scientific Cat#23225 Pierce Reversible Protein Stain Kit for PVDF membranes Thermo Fisher Scientific Cat#24585 Immobilon Forte Western HRP substrate Millipore Cat#WBLUF0020 RNAscope multiplex fluorescent reagent kit v2 Advanced Cell Diagnostics Cat#323285 SingleShot Cell Lysis kit Bio-Rad Cat#1725080 QuantSeq-Pool 3’ mRNA-Seq library prep kit Lexogen Cat#139.96 High sensitivity small DNA Fragment Analysis kit Agilent Technologies Cat#DNF-477-0500 NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles) Illumina Cat#20028315 NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles) Illumina Cat#20028316 miRNeasy Mini kit Qiagen Cat#217004 HL-dsDNase kit ArcticZymes Cat#70800 SMARTer Stranded Total RNA-Seq Kit (v3 - Pico Input 891 Mammalian) Takara Bio Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio Cat#634452 SMARTer RNA Unique Dual Index Kit – 96U Set B Takara Bio Cat#634457 Deposited data Bulk muscle RNA-seq data with single histamine receptor blockade This paper EGAD50000000612 Single-fiber RNA-seq data This paper EGAD50000000612 Single-cell RNA-seq data Turiel et al.22 GSE235143 Bulk muscle RNA-seq data with double histamine receptor blockade Romero et al.60 GSE71972 Data S1 – Source data This paper N/A Software and algorithms R v. 4.2.3 CRAN https://www.r-project.org QuPath v. 0.5.1 QuPath https://qupath.github.io ImageLab v. 6.1 Bio-Rad https://www.bio-rad.com/en-be/product/ image-lab-software?ID=KRE6P5E8Z MassLynx v. 4.2 Waters https://www.waters.com/nextgen/en/ products/informatics-and-software/massspectrometry-software/masslynx-massspectrometry-software.html?srsltid= AfmBOoo1MHb0IwAWzdQXWenTttxdBey79cVBf-UtKPRTbl66nN7OlBw QuantSeq Pool analysis pipeline Lexogen https://github.com/Lexogen-Tools/ quantseqpool_analysis Illustrator v. 29.1 Adobe https://www.adobe.com/be_en/ products/illustrator.html Other Cyclus 2 ergometer RBM Elektronik-automation https://www.cyclus2.com/en/ Excalibur Sport ergometer Lode https://lode-ergometry.com/product/ excalibur-sport/ Fexofenadine Telfast N/A Famotidine Aurobindo Pharma N/A Microdialysis catheter (20 kDa) MDialysis AB #8010514 Cell Metabolism 37, 1–15.e1–e7, April 1, 2025 e2 ..

Western Blot:

RNAscope:

Multiplex Assay:

Lysis:

RNA Sequencing:

Software:

Sequencing:

Article Title: Apparatuses and methods for performing multiple omics analysis and processing analyte mixtures
Article Snippet: Following the transfer of samples into a 384-well plate containing RT-PCR buffer with 3′ SMART-Seq CDS Primer IIA (SMART-Seq® v4 PLUS Kit, TakaRa, cat #R400753); the samples were immediately denatured at 72° C. for 3 min and chilled on ice for at least 2 min. Full length cDNA was generated by adding RT mix to each tube and incubating at 42° C. for 90 min; followed by heat inactivation at 70° C. for 10 min. 18 cycles of cDNA amplification were done to generate enough cDNA for template library according to SMART-Seq® v4 PLUS Kit instruction. .. The SMART-Seq Library Prep Kit and Unique Dual Index Kit (TaKaRa, cat #R400745) were used to generate barcoded template library for sequencing. .. Single-read sequencing of the cDNA libraries with a read length of 150 was performed on NextSeq 550 Sequencing System using NextSeq 500/550 High Output v2 kit (150 cycles, Illumina, cat #20024907).

Article Title: Cortical astrocytes control stress resilience
Article Snippet: 500 pg of RNA was used as input in Takara’s SMARTer® Stranded Total RNA-Seq Kit v2 – Pico, with ribodepletion. .. Sequencing libraries were generated for each sample individually using Takara’s Unique Dual Index Kit. .. Following library preparation, sequencing was performed with Azenta on an Illumina Novaseq.

Clinical Proteomics:

Article Title: Genome-wide cell-free DNA methylation profiling via TAPS for moyamoya disease diagnosis and postoperative collateral formation prediction.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Plasma samples from patients with moyamoya disease (MMD) and atherosclerotic cerebrovascular disease (ACVD) Zhongnan Hospital of Wuhan University Nanjing Drum Tower Hospital, Guangdong Sanjiu Brain Hospital, and Hainan Second People’s Hospital N/A Middle cerebral artery (MCA) samples from patients with MMD Zhongnan Hospital of Wuhan University N/A Lambda DNA Thermo Fisher Cat. #SD0021 Chemicals, peptides, and recombinant proteins α-ketoglutarate Sigma Cat. #K3752 Adenosine triphosphate (ATP) Sigma Cat. #A6419 Dithiothreitol (DTT) Biofroxx Cat. #1111GR005 Ascorbic acid Sigma Cat. #95210 Ammonium iron (II) sulfate Sigma Cat. #215406 Tween 20 Biosharp Cat. #BS100 Sodium acetate solution (pH 4.0) Prepared in-house N/A Pic-borane (pyridine borane) Alfa Aesar Cat. #H66642 EDTA (in blood collection tubes) BD Bioscience N/A mTET2 enzyme (recombinant dioxygenase) Vazyme Cat. #EM301 M.SssI methyltransferase New England Biolabs (NEB) Cat. #M0226L Tris-HCl (pH 8.0) Biosharp Cat. #BL538A HpaII New England Biolabs (NEB) Cat. #R0171 Critical commercial assays High Sensitivity D1000 DNA ScreenTape Agilent Technologies Cat. #5067–5584 High Sensitivity D1000 Reagents Agilent Technologies Cat. #5067–5585 High Sensitivity D5000 DNA ScreenTape Agilent Technologies Cat. # 5067–5592 High Sensitivity D5000 Reagents Agilent Technologies Cat. #5067–5593 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat. #Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat. #Q32855 SMARTer RNA Unique Dual Index Kit-96U Takara Bio Cat. #634452 SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian Takara Bio Cat. #634413 VAHTS Serum/Plasma Circulating DNA Kit Vazyme Cat. #N902 AMPure XP beads Beckman Coulter Cat. #A63881 VAHTS Unique Dual Index Adaptor for Illumina Vazyme Cat. #N34201 VAHTS Universal DNA Library Prep Kit for Illumina V3 Vazyme Cat. #ND607 VAHTS HiFi amplification mix V3 Vazyme Cat. #NE103-P1 VAHTS Library Quantification Kit for Illumina Vazyme Cat. #NQ107-01 Phusion High-Fidelity DNA Polymerase New England Biolabs (NEB) Cat. #M0531 RNAiso Plus Takara Bio Cat. #9109 Oligo Clean & Concentrator-5 kit Zymo Research Cat. #D4061 (Continued on next page) Cell Reports Medicine 7, 103075, October 20, 2026 e1 Article ll OPEN ACCESS ..

Lambda DNA Preparation:

Recombinant:

Sensitivity:

Generated:

RNA sequencing:

Article Title: Cellular deconstruction of the human skeletal muscle microenvironment identifies an exercise-induced histaminergic crosstalk.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 4-imidazole acetic acid hydrochloride Merck Cat#219991 L-histidine Merck Cat#H8000 histamine-a,a,b,b-d4 dihydrochloride Merck Cat#762962 N-methylhistamine Sanbio Cat#19516-10 1-methyl-4-imidazole aceKc acid hydrochloride Sanbio Cat#18815-10 Critical commercial assays Pierce protein assay kit Thermo Fisher Scientific Cat#23225 Pierce Reversible Protein Stain Kit for PVDF membranes Thermo Fisher Scientific Cat#24585 Immobilon Forte Western HRP substrate Millipore Cat#WBLUF0020 RNAscope multiplex fluorescent reagent kit v2 Advanced Cell Diagnostics Cat#323285 SingleShot Cell Lysis kit Bio-Rad Cat#1725080 QuantSeq-Pool 3’ mRNA-Seq library prep kit Lexogen Cat#139.96 High sensitivity small DNA Fragment Analysis kit Agilent Technologies Cat#DNF-477-0500 NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles) Illumina Cat#20028315 NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles) Illumina Cat#20028316 miRNeasy Mini kit Qiagen Cat#217004 HL-dsDNase kit ArcticZymes Cat#70800 SMARTer Stranded Total RNA-Seq Kit (v3 - Pico Input 891 Mammalian) Takara Bio Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio Cat634452 SMARTer RNA Unique Dual Index Kit – 96U Set B Takara Bio Cat#634457 Deposited data Bulk muscle RNA-seq data with single histamine receptor blockade This paper EGAD50000000612 Single-fiber RNA-seq data This paper EGAD50000000612 Single-cell RNA-seq data Turiel et al.22 GSE235143 Bulk muscle RNA-seq data with double histamine receptor blockade Romero et al.60 GSE71972 Data S1 – Source data This paper N/A Software and algorithms R v. 4.2.3 CRAN https://www.r-project.org QuPath v. 0.5.1 QuPath https://qupath.github.io ImageLab v. 6.1 Bio-Rad https://www.bio-rad.com/en-be/product/ image-lab-software?ID=KRE6P5E8Z MassLynx v. 4.2 Waters https://www.waters.com/nextgen/en/ products/informatics-and-software/massspectrometry-software/masslynx-massspectrometry-software.html?srsltid= AfmBOoo1MHb0IwAWzdQXWenTttxdBey79cVBf-UtKPRTbl66nN7OlBw QuantSeq Pool analysis pipeline Lexogen https://github.com/Lexogen-Tools/ quantseqpool_analysis Illustrator v. 29.1 Adobe https://www.adobe.com/be_en/ products/illustrator.html Other Cyclus 2 ergometer RBM Elektronik-automation https://www.cyclus2.com/en/ Excalibur Sport ergometer Lode https://lode-ergometry.com/product/ excalibur-sport/ Fexofenadine Telfast N/A Famotidine Aurobindo Pharma N/A Microdialysis catheter (20 kDa) MDialysis AB #8010514 Cell Metabolism 37, 1–15.e1–e7, April 1, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 4-imidazole acetic acid hydrochloride Merck Cat#219991 L-histidine Merck Cat#H8000 histamine-a,a,b,b-d4 dihydrochloride Merck Cat#762962 N-methylhistamine Sanbio Cat#19516-10 1-methyl-4-imidazole aceKc acid hydrochloride Sanbio Cat#18815-10 Critical commercial assays Pierce protein assay kit Thermo Fisher Scientific Cat#23225 Pierce Reversible Protein Stain Kit for PVDF membranes Thermo Fisher Scientific Cat#24585 Immobilon Forte Western HRP substrate Millipore Cat#WBLUF0020 RNAscope multiplex fluorescent reagent kit v2 Advanced Cell Diagnostics Cat#323285 SingleShot Cell Lysis kit Bio-Rad Cat#1725080 QuantSeq-Pool 3’ mRNA-Seq library prep kit Lexogen Cat#139.96 High sensitivity small DNA Fragment Analysis kit Agilent Technologies Cat#DNF-477-0500 NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles) Illumina Cat#20028315 NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles) Illumina Cat#20028316 miRNeasy Mini kit Qiagen Cat#217004 HL-dsDNase kit ArcticZymes Cat#70800 SMARTer Stranded Total RNA-Seq Kit (v3 - Pico Input 891 Mammalian) Takara Bio Cat#634487 SMARTer RNA Unique Dual Index Kit – 96U Set A Takara Bio Cat#634452 SMARTer RNA Unique Dual Index Kit – 96U Set B Takara Bio Cat#634457 Deposited data Bulk muscle RNA-seq data with single histamine receptor blockade This paper EGAD50000000612 Single-fiber RNA-seq data This paper EGAD50000000612 Single-cell RNA-seq data Turiel et al.22 GSE235143 Bulk muscle RNA-seq data with double histamine receptor blockade Romero et al.60 GSE71972 Data S1 – Source data This paper N/A Software and algorithms R v. 4.2.3 CRAN https://www.r-project.org QuPath v. 0.5.1 QuPath https://qupath.github.io ImageLab v. 6.1 Bio-Rad https://www.bio-rad.com/en-be/product/ image-lab-software?ID=KRE6P5E8Z MassLynx v. 4.2 Waters https://www.waters.com/nextgen/en/ products/informatics-and-software/massspectrometry-software/masslynx-massspectrometry-software.html?srsltid= AfmBOoo1MHb0IwAWzdQXWenTttxdBey79cVBf-UtKPRTbl66nN7OlBw QuantSeq Pool analysis pipeline Lexogen https://github.com/Lexogen-Tools/ quantseqpool_analysis Illustrator v. 29.1 Adobe https://www.adobe.com/be_en/ products/illustrator.html Other Cyclus 2 ergometer RBM Elektronik-automation https://www.cyclus2.com/en/ Excalibur Sport ergometer Lode https://lode-ergometry.com/product/ excalibur-sport/ Fexofenadine Telfast N/A Famotidine Aurobindo Pharma N/A Microdialysis catheter (20 kDa) MDialysis AB #8010514 Cell Metabolism 37, 1–15.e1–e7, April 1, 2025 e2

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