Review



smart seq ultralow version 4 kit  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    TaKaRa smart seq ultralow version 4 kit
    Smart Seq Ultralow Version 4 Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+seq+ht+kit/SMART-Seq+HT+Kit/pmc13083728-63-8-13
    Average 96 stars, based on 467 article reviews
    smart seq ultralow version 4 kit - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Bulk RNA Sequencing:

    Article Title: Human lung organoid modelling of tissue-resident antiviral T cell responses.
    Article Snippet: .. 711 712 Bulk RNA sequencing 713 RNA library preparations, sequencing reactions, and bioinformatic analysis were conducted at 714 GENEWIZ/Azenta Life Sciences LLC. (South Plainfield, NJ, USA) as follows: 715 716 Library Preparation 717 Ultra-low input RNA sequencing library was prepared by using the SMART-Seq HT kit for full-length 718 cDNA synthesis and amplification (Takara, 634436), and Illumina Nextera XT (Illumina,) library was used 719 for sequencing library preparation. ..

    Sequencing:

    Article Title: Human lung organoid modelling of tissue-resident antiviral T cell responses.
    Article Snippet: .. 711 712 Bulk RNA sequencing 713 RNA library preparations, sequencing reactions, and bioinformatic analysis were conducted at 714 GENEWIZ/Azenta Life Sciences LLC. (South Plainfield, NJ, USA) as follows: 715 716 Library Preparation 717 Ultra-low input RNA sequencing library was prepared by using the SMART-Seq HT kit for full-length 718 cDNA synthesis and amplification (Takara, 634436), and Illumina Nextera XT (Illumina,) library was used 719 for sequencing library preparation. ..

    Analysis:

    Article Title: Human lung organoid modelling of tissue-resident antiviral T cell responses.
    Article Snippet: .. 711 712 Bulk RNA sequencing 713 RNA library preparations, sequencing reactions, and bioinformatic analysis were conducted at 714 GENEWIZ/Azenta Life Sciences LLC. (South Plainfield, NJ, USA) as follows: 715 716 Library Preparation 717 Ultra-low input RNA sequencing library was prepared by using the SMART-Seq HT kit for full-length 718 cDNA synthesis and amplification (Takara, 634436), and Illumina Nextera XT (Illumina,) library was used 719 for sequencing library preparation. ..

    RNA Sequencing:

    Article Title: Human lung organoid modelling of tissue-resident antiviral T cell responses.
    Article Snippet: .. 711 712 Bulk RNA sequencing 713 RNA library preparations, sequencing reactions, and bioinformatic analysis were conducted at 714 GENEWIZ/Azenta Life Sciences LLC. (South Plainfield, NJ, USA) as follows: 715 716 Library Preparation 717 Ultra-low input RNA sequencing library was prepared by using the SMART-Seq HT kit for full-length 718 cDNA synthesis and amplification (Takara, 634436), and Illumina Nextera XT (Illumina,) library was used 719 for sequencing library preparation. ..

    Article Title: Phenotypic and transcriptional changes in human brain pericytes induced by phorbol 12-myristate 13-acetate
    Article Snippet: RNA 153 integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies), and 154 samples with an RNA integrity number (RIN) greater than 9 were used for sequencing. .. 155 RNA-seq libraries were prepared with the SMART-Seq HT Kit (Clontech, Santa Clara, 156 CA, USA) followed by the Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, 157 USA). .. Sequencing was performed on a NovaSeq X Plus platform (Illumina) to a depth of 158 approximately 30 million paired-end reads (100 bp) per sample.

    cDNA Synthesis:

    Article Title: Human lung organoid modelling of tissue-resident antiviral T cell responses.
    Article Snippet: .. 711 712 Bulk RNA sequencing 713 RNA library preparations, sequencing reactions, and bioinformatic analysis were conducted at 714 GENEWIZ/Azenta Life Sciences LLC. (South Plainfield, NJ, USA) as follows: 715 716 Library Preparation 717 Ultra-low input RNA sequencing library was prepared by using the SMART-Seq HT kit for full-length 718 cDNA synthesis and amplification (Takara, 634436), and Illumina Nextera XT (Illumina,) library was used 719 for sequencing library preparation. ..

    Amplification:

    Article Title: Human lung organoid modelling of tissue-resident antiviral T cell responses.
    Article Snippet: .. 711 712 Bulk RNA sequencing 713 RNA library preparations, sequencing reactions, and bioinformatic analysis were conducted at 714 GENEWIZ/Azenta Life Sciences LLC. (South Plainfield, NJ, USA) as follows: 715 716 Library Preparation 717 Ultra-low input RNA sequencing library was prepared by using the SMART-Seq HT kit for full-length 718 cDNA synthesis and amplification (Takara, 634436), and Illumina Nextera XT (Illumina,) library was used 719 for sequencing library preparation. ..

    Article Title: Rbm24 promotes outer hair cell survival through Insm1 repression while independently regulating hair bundle morphogenesis.
    Article Snippet: The inactivation of Rbm24, an RNA-binding protein, leads to cell death and hair bundle defects in cochlear outer hair cells (OHCs).. However, the underlying molecular mechanisms remain unclear.. To address this, we have performed comprehensive transcriptomic profiling of purified wild-type andRbm24−/−mouseOHCs at postnatal day 7 (P7).

    Article Title: An SPP1-SOCS1 pathway constrains interferon responses in tumor-associated macrophages and shapes an immunosuppressive tumor microenvironment.
    Article Snippet: .. The T7 DNA templates were amplified following the instructions of SMART-Seq HT Kit (Takara; 634436), and the PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN; 28704) according to the manufacturer’s instructions. .. The mRNA fragments were then transcribed in vitro using the mMESSAGE mMACHINETM T7 Transcription Kit according to the manufacturer’s protocol (Thermo Scientific, AM1344).

    Reverse Transcription:

    Article Title: Rbm24 promotes outer hair cell survival through Insm1 repression while independently regulating hair bundle morphogenesis.
    Article Snippet: The inactivation of Rbm24, an RNA-binding protein, leads to cell death and hair bundle defects in cochlear outer hair cells (OHCs).. However, the underlying molecular mechanisms remain unclear.. To address this, we have performed comprehensive transcriptomic profiling of purified wild-type andRbm24−/−mouseOHCs at postnatal day 7 (P7).

    Article Title: A preliminary integrated morphologic and transcriptomic analysis of denervation remodeling in rat laryngeal and hindlimb muscles
    Article Snippet: Total RNA was isolated from denervated and control muscle tissues using the RNeasy Mini Kit (QIAGEN, Hilden, Germany) and RNA integrity was verified using an Agilent Bioanalyzer. .. Samples ≤1ng were reverse transcribed into complementary DNA (cDNA) using the SMART-Seq HT Kit (Takara Bio USA, Mountain View, CA, USA), with 13 PCR cycles. .. cDNA was quantified using the Invitrogen Quant-iT system (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA) and diluted to 0.3ng/μL for library preparation.

    Polymerase Chain Reaction:

    Article Title: A preliminary integrated morphologic and transcriptomic analysis of denervation remodeling in rat laryngeal and hindlimb muscles
    Article Snippet: Total RNA was isolated from denervated and control muscle tissues using the RNeasy Mini Kit (QIAGEN, Hilden, Germany) and RNA integrity was verified using an Agilent Bioanalyzer. .. Samples ≤1ng were reverse transcribed into complementary DNA (cDNA) using the SMART-Seq HT Kit (Takara Bio USA, Mountain View, CA, USA), with 13 PCR cycles. .. cDNA was quantified using the Invitrogen Quant-iT system (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA) and diluted to 0.3ng/μL for library preparation.

    Article Title: An SPP1-SOCS1 pathway constrains interferon responses in tumor-associated macrophages and shapes an immunosuppressive tumor microenvironment.
    Article Snippet: .. The T7 DNA templates were amplified following the instructions of SMART-Seq HT Kit (Takara; 634436), and the PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN; 28704) according to the manufacturer’s instructions. .. The mRNA fragments were then transcribed in vitro using the mMESSAGE mMACHINETM T7 Transcription Kit according to the manufacturer’s protocol (Thermo Scientific, AM1344).

    Construct:

    Article Title: SENP1 facilitates the adaptation of colonic non-lymphoid tissue Treg cells and restrains intestinal inflammation.
    Article Snippet: RNA quality was assessed using the Bioanalyzer 2100 platform (Agilent Technologies). .. Libraries were constructed using the SMART-Seq® HT Kit (Takara Bio USA) and sequenced on an Illumina NovaSeq 6000. ..

    Purification:

    Article Title: An SPP1-SOCS1 pathway constrains interferon responses in tumor-associated macrophages and shapes an immunosuppressive tumor microenvironment.
    Article Snippet: .. The T7 DNA templates were amplified following the instructions of SMART-Seq HT Kit (Takara; 634436), and the PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN; 28704) according to the manufacturer’s instructions. .. The mRNA fragments were then transcribed in vitro using the mMESSAGE mMACHINETM T7 Transcription Kit according to the manufacturer’s protocol (Thermo Scientific, AM1344).

    Gel Extraction:

    Article Title: An SPP1-SOCS1 pathway constrains interferon responses in tumor-associated macrophages and shapes an immunosuppressive tumor microenvironment.
    Article Snippet: .. The T7 DNA templates were amplified following the instructions of SMART-Seq HT Kit (Takara; 634436), and the PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN; 28704) according to the manufacturer’s instructions. .. The mRNA fragments were then transcribed in vitro using the mMESSAGE mMACHINETM T7 Transcription Kit according to the manufacturer’s protocol (Thermo Scientific, AM1344).



    Similar Products

    96
    TaKaRa smart seq ht kit
    Smart Seq Ht Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+seq+ht+kit/SMART-Seq+HT+Kit/pm42844476-442-43-53
    Average 96 stars, based on 1 article reviews
    smart seq ht kit - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    TaKaRa smartseq ht kit
    Smartseq Ht Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+seq+ht+kit/SMART-Seq+HT+Kit/pm42121178-37-7-10
    Average 96 stars, based on 1 article reviews
    smartseq ht kit - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    TaKaRa smart seq ht plus kit
    Smart Seq Ht Plus Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+seq+ht+kit/SMART-Seq+HT+PLUS+Kit/10__1016_slash_j__reth__2026__101125-58-16-21
    Average 96 stars, based on 1 article reviews
    smart seq ht plus kit - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    Image Search Results