smart seq stranded kit (TaKaRa)

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TaKaRa smart seq stranded kit
Smart Seq Stranded Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smart+seq+ht+kit/SMART-Seq+Stranded+Kit/pm42763384-51-6-9
Average 96 stars, based on 344 article reviews

smart seq stranded kit - by Bioz Stars, 2026-11

96/100 stars

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Article Title: AMPK-p38 axis converts human pluripotent stem cells to naive state
Article Snippet: SMART-Seq Stranded Kit , Takara Bio , Cat# 634442.

cDNA Synthesis:

Article Title: Juvenile social isolation induces gene expression alterations and histone modification dysregulations in nucleus accumbens (NAc) neurons
Article Snippet: .. After removing the supernatant, nuclei were resuspended in 3.5 μl of water and transferred to a new tube for cDNA synthesis and library preparation using the SMART-seq Stranded Kit (Takara, #634447) according to the manufacturer’s protocol. .. Library concentrations were assessed using the TapeStation system (Agilent), and sequencing was performed on the DNB-seq platform (MGI) to obtain 50-base single-end reads.

RNA sequencing:

Article Title: Intraductal Papillary Squamous Neoplasm (IPSN) of the Pancreas: Histologic and Molecular Characterization of a Novel and Distinct Intraductal Cancer Precursor.
Article Snippet: .. RNAseq was performed using SMART-Seq Stranded kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions. .. The RNA libraries were sequenced on NovaSeq 6000 (Illumina, San Diego, California).

Sequencing:

Article Title: circPDE4B downregulation triggers GEMIN5‑dependent translational stress response and autophagy to reduce MAPT pathology.
Article Snippet: .. The sequencing libraries were prepared using SMART-Seq Total RNA High Input kit following manufacturer’s instructions (Takara Bio USA, Inc., Cat# 635046). .. purified using the Qiagen RNeasy Plus Mini Kit (Qiagen, Cat# 74134) according to the manufacturer’s instructions

Article Title: Pleuroparenchymal fibroelastosis in a monogenic mitochondrial DNA depletion syndrome.
Article Snippet: .. RNA libraries were prepared for sequencing using Takara SMART-Seq stranded total RNA Kit and processed on an Illumina NovaseqXplus platform with standard paired-end runs. ..

Article Title: Tumor-associated calcium signal transducer 2 expression as a promising therapeutic target in esophageal squamous cell carcinoma: a combined the Cancer Genome Atlas and an institutional cohort.
Article Snippet: Briefly, total RNA was extracted from two FFPE tissue slices using an miRNeasy FFPE kit (Qiagen) [20]. .. Whole-transcriptome sequencing was performed using the SMART-Seq Stranded kit (Takara Bio Europe) for library preparation and NextSeq 500, single end 75 bp (Illumina) for sequencing [21]. ..

Article Title: A multi-omics analysis of advanced triple-negative breast cancer identifies predictors to chemo-immunotherapy and on-treatment dynamics.
Article Snippet: .. CeGaT used KAPA RNA Hyper Prep (Roche) or SMART-Seq Stranded (Takara Bio) for the library preparation and sequencing on the NovaSeq6000, at 2 × 100 bp, at a depth of 100 Mio paired end reads. ..

RNA Sequencing:

Article Title: Tofogliflozin alters amino acid metabolism in gut microbiota linked to hepatic transcriptomic signatures in MASLD.
Article Snippet: Background A deeper understanding of the relationship between dysbiotic gut microbiota and liver tissue-level molecular and histopathological phenotypes in metabolic dysfunction-associated steatotic liver disease (MASLD) remains needed.. We aimed to characterize the associations between gut microbial metabolic functions and treatment responses in participants with MASLD.. Methods We performed a prespecified sub-analysis of a randomized controlled trial comparing the sodium–glucose cotransporter 2 inhibitor (SGLT2i) tofogliflozin and the sulfonylurea (SU) glimepiride in participants with MASLD and type 2 diabetes (ClinicalTrials.gov NCT02649465).

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