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slide seq v2  (TaKaRa)


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    Structured Review

    TaKaRa slide seq v2
    Adventitial fibroblast in human arteries (A) Featureplot of gene score of ECM- and collagen-related genes demonstrating enrichment in AdvFib. (B) Most specific adventitial (Adv) fibroblast marker, PI16 , RNA expression visualized on the RNA-seq UMAP <t>(left),</t> <t>slide-seq</t> spatial transcriptomics map (middle), and RNAscope (right), confirming the spatial identity of PI16-expressing fibroblasts. (C) UMAP visualization of canonical fibroblast marker genes with diffuse expression pattern, including S100A4 , PDGFRA , COL1A1 , and GLI1 , compared to additional new markers SFRP2 and MFAP5. (D) Relative specificity and sensitivities of commonly used and newly identified “fibroblast markers.” (E) Violin plots showing differential expression of key genes implicated in aneurysm FBN1 , TGFBR2 , LOX , FBLN1 , and MFAP5 expressed mostly in AdvFib. See also and .
    Slide Seq V2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slide+seq+v2/Seeker+Spatial+Transcriptomics+Kit/pmc12802643-20-0-3
    Average 95 stars, based on 159 article reviews
    slide seq v2 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "A cell and transcriptome atlas of human arterial vasculature"

    Article Title: A cell and transcriptome atlas of human arterial vasculature

    Journal: Cell Genomics

    doi: 10.1016/j.xgen.2025.101034

    Adventitial fibroblast in human arteries (A) Featureplot of gene score of ECM- and collagen-related genes demonstrating enrichment in AdvFib. (B) Most specific adventitial (Adv) fibroblast marker, PI16 , RNA expression visualized on the RNA-seq UMAP (left), slide-seq spatial transcriptomics map (middle), and RNAscope (right), confirming the spatial identity of PI16-expressing fibroblasts. (C) UMAP visualization of canonical fibroblast marker genes with diffuse expression pattern, including S100A4 , PDGFRA , COL1A1 , and GLI1 , compared to additional new markers SFRP2 and MFAP5. (D) Relative specificity and sensitivities of commonly used and newly identified “fibroblast markers.” (E) Violin plots showing differential expression of key genes implicated in aneurysm FBN1 , TGFBR2 , LOX , FBLN1 , and MFAP5 expressed mostly in AdvFib. See also and .
    Figure Legend Snippet: Adventitial fibroblast in human arteries (A) Featureplot of gene score of ECM- and collagen-related genes demonstrating enrichment in AdvFib. (B) Most specific adventitial (Adv) fibroblast marker, PI16 , RNA expression visualized on the RNA-seq UMAP (left), slide-seq spatial transcriptomics map (middle), and RNAscope (right), confirming the spatial identity of PI16-expressing fibroblasts. (C) UMAP visualization of canonical fibroblast marker genes with diffuse expression pattern, including S100A4 , PDGFRA , COL1A1 , and GLI1 , compared to additional new markers SFRP2 and MFAP5. (D) Relative specificity and sensitivities of commonly used and newly identified “fibroblast markers.” (E) Violin plots showing differential expression of key genes implicated in aneurysm FBN1 , TGFBR2 , LOX , FBLN1 , and MFAP5 expressed mostly in AdvFib. See also and .

    Techniques Used: Marker, RNA Expression, RNA Sequencing, RNAscope, Expressing, Quantitative Proteomics

    Related Articles

    Spatial Transcriptomics:

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection
    Article Snippet: .. Slide-seq spatial transcriptomics experiment was performed using the Curio Seeker Kit (Curio Bioscience) according to manufacturer instructions. .. In brief, a 10-μm thickness tissue section from each collected fresh-frozen PLN was mounted on a 3-mm x 3-mm spatially indexed bead surface Curio Seeker tile.

    Article Title: BRRIAR lncRNA alters breast cancer risk by modulating interferon signaling in cis and in trans
    Article Snippet: .. Fig. 6 BRRIAR expression and immune activation in patient-derived samples. a High-resolution spatial transcriptomics map of one ER + breast tumor generated using the Curio Seeker platform. b Corresponding BRRIAR -positive cell states. c Spatial neighborhood analysis of BRRIAR -positive cells. ..

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection
    Article Snippet: .. The AIR-SPACE protocol begins with a 10-μm tissue section mounted onto a spatial transcriptomics array (Curio Seeker, Materials and Methods ). ..

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection.
    Article Snippet: .. The AIR-SPACE protocol begins with a 10-μm tissue section mounted onto a spatial transcriptomics array (Curio Seeker, Materials and Methods ). ..

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection.
    Article Snippet: Slide-Seq Spatial Transcriptomics Library Preparation and Sequencing. .. Slide-seq spatial transcriptomics experiment was performed using the Curio Seeker Kit (Curio Bioscience) according to manufacturer instructions. .. In brief, a 10-μm thickness tissue section from each collected fresh-frozen PLN was mounted on a 3-mm x 3-mm spatially indexed bead surface Curio Seeker tile.

    Expressing:

    Article Title: BRRIAR lncRNA alters breast cancer risk by modulating interferon signaling in cis and in trans
    Article Snippet: .. Fig. 6 BRRIAR expression and immune activation in patient-derived samples. a High-resolution spatial transcriptomics map of one ER + breast tumor generated using the Curio Seeker platform. b Corresponding BRRIAR -positive cell states. c Spatial neighborhood analysis of BRRIAR -positive cells. ..

    Activation Assay:

    Article Title: BRRIAR lncRNA alters breast cancer risk by modulating interferon signaling in cis and in trans
    Article Snippet: .. Fig. 6 BRRIAR expression and immune activation in patient-derived samples. a High-resolution spatial transcriptomics map of one ER + breast tumor generated using the Curio Seeker platform. b Corresponding BRRIAR -positive cell states. c Spatial neighborhood analysis of BRRIAR -positive cells. ..

    Generated:

    Article Title: BRRIAR lncRNA alters breast cancer risk by modulating interferon signaling in cis and in trans
    Article Snippet: .. Fig. 6 BRRIAR expression and immune activation in patient-derived samples. a High-resolution spatial transcriptomics map of one ER + breast tumor generated using the Curio Seeker platform. b Corresponding BRRIAR -positive cell states. c Spatial neighborhood analysis of BRRIAR -positive cells. ..



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    Adventitial fibroblast in human arteries (A) Featureplot of gene score of ECM- and collagen-related genes demonstrating enrichment in AdvFib. (B) Most specific adventitial (Adv) fibroblast marker, PI16 , RNA expression visualized on the RNA-seq UMAP <t>(left),</t> <t>slide-seq</t> spatial transcriptomics map (middle), and RNAscope (right), confirming the spatial identity of PI16-expressing fibroblasts. (C) UMAP visualization of canonical fibroblast marker genes with diffuse expression pattern, including S100A4 , PDGFRA , COL1A1 , and GLI1 , compared to additional new markers SFRP2 and MFAP5. (D) Relative specificity and sensitivities of commonly used and newly identified “fibroblast markers.” (E) Violin plots showing differential expression of key genes implicated in aneurysm FBN1 , TGFBR2 , LOX , FBLN1 , and MFAP5 expressed mostly in AdvFib. See also and .
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    Adventitial fibroblast in human arteries (A) Featureplot of gene score of ECM- and collagen-related genes demonstrating enrichment in AdvFib. (B) Most specific adventitial (Adv) fibroblast marker, PI16 , RNA expression visualized on the RNA-seq UMAP <t>(left),</t> <t>slide-seq</t> spatial transcriptomics map (middle), and RNAscope (right), confirming the spatial identity of PI16-expressing fibroblasts. (C) UMAP visualization of canonical fibroblast marker genes with diffuse expression pattern, including S100A4 , PDGFRA , COL1A1 , and GLI1 , compared to additional new markers SFRP2 and MFAP5. (D) Relative specificity and sensitivities of commonly used and newly identified “fibroblast markers.” (E) Violin plots showing differential expression of key genes implicated in aneurysm FBN1 , TGFBR2 , LOX , FBLN1 , and MFAP5 expressed mostly in AdvFib. See also and .
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    Image Search Results


    Adventitial fibroblast in human arteries (A) Featureplot of gene score of ECM- and collagen-related genes demonstrating enrichment in AdvFib. (B) Most specific adventitial (Adv) fibroblast marker, PI16 , RNA expression visualized on the RNA-seq UMAP (left), slide-seq spatial transcriptomics map (middle), and RNAscope (right), confirming the spatial identity of PI16-expressing fibroblasts. (C) UMAP visualization of canonical fibroblast marker genes with diffuse expression pattern, including S100A4 , PDGFRA , COL1A1 , and GLI1 , compared to additional new markers SFRP2 and MFAP5. (D) Relative specificity and sensitivities of commonly used and newly identified “fibroblast markers.” (E) Violin plots showing differential expression of key genes implicated in aneurysm FBN1 , TGFBR2 , LOX , FBLN1 , and MFAP5 expressed mostly in AdvFib. See also and .

    Journal: Cell Genomics

    Article Title: A cell and transcriptome atlas of human arterial vasculature

    doi: 10.1016/j.xgen.2025.101034

    Figure Lengend Snippet: Adventitial fibroblast in human arteries (A) Featureplot of gene score of ECM- and collagen-related genes demonstrating enrichment in AdvFib. (B) Most specific adventitial (Adv) fibroblast marker, PI16 , RNA expression visualized on the RNA-seq UMAP (left), slide-seq spatial transcriptomics map (middle), and RNAscope (right), confirming the spatial identity of PI16-expressing fibroblasts. (C) UMAP visualization of canonical fibroblast marker genes with diffuse expression pattern, including S100A4 , PDGFRA , COL1A1 , and GLI1 , compared to additional new markers SFRP2 and MFAP5. (D) Relative specificity and sensitivities of commonly used and newly identified “fibroblast markers.” (E) Violin plots showing differential expression of key genes implicated in aneurysm FBN1 , TGFBR2 , LOX , FBLN1 , and MFAP5 expressed mostly in AdvFib. See also and .

    Article Snippet: Slide-Seq V2 , Curio , #CB00030 v3.1.

    Techniques: Marker, RNA Expression, RNA Sequencing, RNAscope, Expressing, Quantitative Proteomics