Review



rabbit polyclonal antihuman vegf antiserum  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Santa Cruz Biotechnology rabbit polyclonal antihuman vegf antiserum
    FIG. 5. Effect of Dex treatment on adrenal cortex vasculature and <t>VEGF</t> expression. Sections of adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14) were immunostained for CD31 (A) or VEGF (B). The dotted lines indicate the border between adrenal cortex and medulla. Immunoreactivity is indicated by brown staining. Data from one representative experiment are shown. Similar results were obtained in three independent experiments.
    Rabbit Polyclonal Antihuman Vegf Antiserum, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slide+seq+spatial+transcriptomics+experiment/VEGF/pm15178648-67-10-16
    Average 96 stars, based on 3193 article reviews
    rabbit polyclonal antihuman vegf antiserum - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Dual hormonal regulation of endocrine tissue mass and vasculature by adrenocorticotropin in the adrenal cortex."

    Article Title: Dual hormonal regulation of endocrine tissue mass and vasculature by adrenocorticotropin in the adrenal cortex.

    Journal: Endocrinology

    doi: 10.1210/en.2004-0179

    FIG. 5. Effect of Dex treatment on adrenal cortex vasculature and VEGF expression. Sections of adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14) were immunostained for CD31 (A) or VEGF (B). The dotted lines indicate the border between adrenal cortex and medulla. Immunoreactivity is indicated by brown staining. Data from one representative experiment are shown. Similar results were obtained in three independent experiments.
    Figure Legend Snippet: FIG. 5. Effect of Dex treatment on adrenal cortex vasculature and VEGF expression. Sections of adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14) were immunostained for CD31 (A) or VEGF (B). The dotted lines indicate the border between adrenal cortex and medulla. Immunoreactivity is indicated by brown staining. Data from one representative experiment are shown. Similar results were obtained in three independent experiments.

    Techniques Used: Expressing, Staining

    FIG. 6. Effects of Dex treatment on the expression of endocrine and endothelial cell markers. A, RT-PCR analysis of the expression of VEGF-A isoforms (the two bands correspond to specific amplification products of the VEGF120 and VEGF164 isoforms), VEGF-Rs (VEGF-R1/flt-1, VEGF-R2/flk-1, and neuropilin-1), endocrine cell markers (ACTH receptor/MC2-R, HDL receptor/SR-B1), and endothelial cell markers (PECAM and VE-cadherin) in the adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14). RT-PCR amplification of HPRT mRNA was used as an internal standard for the normalization of the samples. The results from one representative experiment are shown. Similar results were obtained in three independent experiments. B and C, Quantitative determination of adrenal VEGF-A and VEGF-Rs mRNA levels by real-time RT-PCR was performed as described in Materials and Methods. The ratio of level of expression of the gene of interest to that of GAPDH was normalized to 1 in control adrenals. The relative quantities of the following mRNAs were plotted as a function of time of Dex treatment: VEGF-A (B); VEGF-R1 (C, solid line), VEGF-R2 (C, continuous dotted line), and neuropilin-1 (C, discontinuous dotted line). Each value represents the mean SEM from 10 to 15 mouse adrenals collected in three independent experiments.
    Figure Legend Snippet: FIG. 6. Effects of Dex treatment on the expression of endocrine and endothelial cell markers. A, RT-PCR analysis of the expression of VEGF-A isoforms (the two bands correspond to specific amplification products of the VEGF120 and VEGF164 isoforms), VEGF-Rs (VEGF-R1/flt-1, VEGF-R2/flk-1, and neuropilin-1), endocrine cell markers (ACTH receptor/MC2-R, HDL receptor/SR-B1), and endothelial cell markers (PECAM and VE-cadherin) in the adrenal glands from mice treated with 1 mg/kgd Dex for 0–14 d (D0 to D14). RT-PCR amplification of HPRT mRNA was used as an internal standard for the normalization of the samples. The results from one representative experiment are shown. Similar results were obtained in three independent experiments. B and C, Quantitative determination of adrenal VEGF-A and VEGF-Rs mRNA levels by real-time RT-PCR was performed as described in Materials and Methods. The ratio of level of expression of the gene of interest to that of GAPDH was normalized to 1 in control adrenals. The relative quantities of the following mRNAs were plotted as a function of time of Dex treatment: VEGF-A (B); VEGF-R1 (C, solid line), VEGF-R2 (C, continuous dotted line), and neuropilin-1 (C, discontinuous dotted line). Each value represents the mean SEM from 10 to 15 mouse adrenals collected in three independent experiments.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Quantitative RT-PCR, Control

    Related Articles

    Immunofluorescence:

    Article Title: Myeloid HIF-1α Sustains Hypoxic Fibrotic Fronts and Drives Pulmonary Fibrosis
    Article Snippet: The lung tissues were then frozen and sectioned and stained with the hypoxyprobe-1 for anti-pimonidazole (Probe, green) and anti-mouse CD68, or αSMA, counterstained with DAPI. .. Antibodies for mCD68 (FA-11, abcam), mCD206 (2A6A10, proteintech), cleaved caspase-3 (Asp175, 5A1E), αSMA (abcam), Collage I (Abcam), TGFβ (Abcam), cMYC (Cell signaling Technology) and VEGF (Santa Cruz) were used for immunofluorescence. .. HIF-1α antibody (GeneTex) was used for immunochemistry Staining.

    Incubation:

    Article Title: Connexin46-enriched extracellular vesicles facilitate cargo delivery and promote angiogenesis
    Article Snippet: .. Cells not incubated with EVs Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-72274-8 and treated with 100 μM of vascular endothelial growth factor (VEGF; Santa Cruz Biotechnology; #sc-4570) were used as a positive control. .. Generation and validation of luciferase-expressing MCF-7 cells To transduce MCF-7 cells with luciferase, HEK293T cells (ATCC, CRL-11268) at 70% confluence were co-transfected with three packaging plasmids pRSV-REV (Addgene, Watertown, MA, USA; #12253), pMD.G (Addgene; #12259), and pMDLg/pRRE (Addgene; #12251) together with the lentiviral reporter vector pHIV-Luciferase (Addgene; #21375) using Lipofectamine 2000 (Invitrogen, ThermoFisher Scientific; #52887, lot 1404702) according to the manufacturer’s instructions.

    Article Title: Allogeneic Human Bone Marrow‐Derived Mesenchymal Stem Cells Accelerate Diabetic Wound Healing via Inflammation Suppression and Angiogenesis Promotion: A Preclinical Study
    Article Snippet: Immunohistochemistry (IHC) semiquantitative staining was carried out using the horseradish peroxidase–diaminobenzidine (HRP–DAB) staining kit (R&D Inc., Minneapolis, MN, USA), following established protocols [ , ]. .. Polyclonal antibodies targeting CD45, vascular endothelial growth factor (VEGF), Ki‐67, epidermal growth factor (EGF), and prolyl 4‐hydroxylase (P4H) (Santa Cruz, Santa Cruz, CA, USA) served as primary antibodies, and the sections were incubated with these antibodies at a 1:100 dilution in phosphate‐buffered saline for 1 h. Subsequently, the sections underwent incubation with biotinylated goat anti‐rabbit antibodies for 30 min. ..

    Article Title: Metabolic-dysfunction-associated steatohepatitis impairs cochlear integrity and delays auditory recovery after noise trauma.
    Article Snippet: Equal amounts of protein (20–30 μg per sample) were separated by 8-12% SDS–PAGE and transferred onto PVDF membranes (Millipore, MA, USA). .. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against VEGF (Santa Cruz Biotechnology, TX, USA), PDGFRβ (Abcam), IL-6 (Santa Cruz Biotechnology), IL-1β (Abcam), LCN2/NGAL (Abcam), and βactin (Cell Signaling Technology, MA, USA). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature.

    Article Title: Local Lung HIF-1α and VEGF Activation to Reverse Emphysema by a Sulfated Caffeic Acid Dehydropolymer
    Article Snippet: .. The membranes were incubated with primary antibodies against: (1) PCNA (1:2000; Cell Signaling Technology); (2) cleaved caspase-3 (cCasp3; 1:500; Cell Signaling Technology); (3) HIF-1α (1:1000; Thermo Fisher Scientific); and (4) VEGF (1:100; Santa Cruz Biotechnology, Dallas, TX, USA). .. Target proteins were detected using an HRP-conjugated anti-IgG antibody, followed by reaction with SuperSignal West Pico chemiluminescent substrate (Pierce, Rockford, IL, USA).

    Positive Control:

    Article Title: Connexin46-enriched extracellular vesicles facilitate cargo delivery and promote angiogenesis
    Article Snippet: .. Cells not incubated with EVs Article in Press Scientific Reports https://doi.org/10.1038/s41598-026-72274-8 and treated with 100 μM of vascular endothelial growth factor (VEGF; Santa Cruz Biotechnology; #sc-4570) were used as a positive control. .. Generation and validation of luciferase-expressing MCF-7 cells To transduce MCF-7 cells with luciferase, HEK293T cells (ATCC, CRL-11268) at 70% confluence were co-transfected with three packaging plasmids pRSV-REV (Addgene, Watertown, MA, USA; #12253), pMD.G (Addgene; #12259), and pMDLg/pRRE (Addgene; #12251) together with the lentiviral reporter vector pHIV-Luciferase (Addgene; #21375) using Lipofectamine 2000 (Invitrogen, ThermoFisher Scientific; #52887, lot 1404702) according to the manufacturer’s instructions.

    Saline:

    Article Title: Allogeneic Human Bone Marrow‐Derived Mesenchymal Stem Cells Accelerate Diabetic Wound Healing via Inflammation Suppression and Angiogenesis Promotion: A Preclinical Study
    Article Snippet: Immunohistochemistry (IHC) semiquantitative staining was carried out using the horseradish peroxidase–diaminobenzidine (HRP–DAB) staining kit (R&D Inc., Minneapolis, MN, USA), following established protocols [ , ]. .. Polyclonal antibodies targeting CD45, vascular endothelial growth factor (VEGF), Ki‐67, epidermal growth factor (EGF), and prolyl 4‐hydroxylase (P4H) (Santa Cruz, Santa Cruz, CA, USA) served as primary antibodies, and the sections were incubated with these antibodies at a 1:100 dilution in phosphate‐buffered saline for 1 h. Subsequently, the sections underwent incubation with biotinylated goat anti‐rabbit antibodies for 30 min. ..

    Immunohistochemical staining:

    Article Title: Correlation Between Molecular Genetic Analysis and Nuclear Pleomorphism in Long-Term Recurrent and Metastatic Chordoma.
    Article Snippet: .. Immunohistochemical staining was performed on all samples for brachyury (EPR18113, Abcam limited, Cambridge, UK), Ki-67 (MIB-1, Dako Corporation, Agilent, Santa Clara, CA, USA), p53 (453M-95, DO7, Cell Marque, Rocklin, CA, USA), S100 (Z0331, Dako Corporation, Agilent), vimentin (Vim384, Dako Corporation, Agilent), E-cadherin (EP700Y, Cell Marque, Darmstadt, Germany), pancytokeratin (PCK, AE1/3, Leica Biosystems, Nußloch, Germany), EGFR (E30, Dako Corporation, Agilent), VEGF (VG-1, Santa Cruz Biotechnology, Dallas, TX, USA), SMARCB1 (25/BAF47, BD Biosciences, Franklin Lakes, NJ, USA), https://doi.org/10.3390/cancers18060898 lamin A/C (EP4520-16, Abcam Cambridge Biomedical Campus, Cambridge, UK), PD-1 (315M-95, Cell Marque, Rocklin, CA, USA) and PD-L1 (M3653, Dako Corporation, Agilent). ..

    Article Title: Immunohistochemical Evaluation of Non-Thermal Plasma-Treated Xenogenic Bone Graft for Enhanced Bone Regeneration in a Rabbit Calvarial Defect Model
    Article Snippet: .. Immunohistochemical analysis was performed using antibodies against proliferating cell nuclear antigen (PCNA; mouse monoclonal, PC10, Santa Cruz Biotechnology, Dallas, TX, USA; 1:200), vascular endothelial growth factor (VEGF; rabbit polyclonal, A-20, Santa Cruz Biotechnology, Dallas, TX, USA; 1:100), platelet endothelial cell adhesion molecule-1 (PECAM-1; goat polyclonal, M-20, Santa Cruz Biotechnology, Dallas, TX, USA; 1:100), collagen I (rabbit monoclonal, ab138492, Abcam, Cambridge, UK; 1:100), collagen III (mouse monoclonal, FH-7A, Abcam, Cambridge, UK; 1:500), alkaline phosphatase (ALP; rabbit monoclonal, ab307726, Abcam, Cambridge, UK; 1:500), bone morphogenetic protein 2 (BMP2; mouse monoclonal, ab6285, Abcam, Cambridge, UK; 1:200), BMP4 (rabbit polyclonal, ab39973, Abcam, Cambridge, UK; 1:500), BMP7 (rabbit polyclonal, ab84684, Abcam, Cambridge, UK; 1:500), osteocalcin (rabbit polyclonal, ab198228, Abcam, Cambridge, UK; 1:200), and osteopontin (mouse monoclonal, ab69498, Abcam, Cambridge, UK; 1:100). ..

    Article Title: Correlation Between Molecular Genetic Analysis and Nuclear Pleomorphism in Long-Term Recurrent and Metastatic Chordoma
    Article Snippet: .. Immunohistochemical staining was performed on all samples for brachyury ( EPR18113 , Abcam limited, Cambridge, UK), Ki-67 (MIB-1, Dako Corporation, Agilent, Santa Clara, CA, USA), p53 (453M-95, DO7, Cell Marque, Rocklin, CA, USA), S100 (Z0331, Dako Corporation, Agilent), vimentin (Vim384, Dako Corporation, Agilent), E-cadherin (EP700Y, Cell Marque, Darmstadt, Germany), pancytokeratin (PCK, AE1/3, Leica Biosystems, Nußloch, Germany), EGFR (E30, Dako Corporation, Agilent), VEGF (VG-1, Santa Cruz Biotechnology, Dallas, TX, USA), SMARCB1 (25/BAF47, BD Biosciences, Franklin Lakes, NJ, USA), lamin A/C (EP4520-16, Abcam Cambridge Biomedical Campus, Cambridge, UK), PD-1 (315M-95, Cell Marque, Rocklin, CA, USA) and PD-L1 (M3653, Dako Corporation, Agilent). ..

    Staining:

    Article Title: Correlation Between Molecular Genetic Analysis and Nuclear Pleomorphism in Long-Term Recurrent and Metastatic Chordoma.
    Article Snippet: .. Immunohistochemical staining was performed on all samples for brachyury (EPR18113, Abcam limited, Cambridge, UK), Ki-67 (MIB-1, Dako Corporation, Agilent, Santa Clara, CA, USA), p53 (453M-95, DO7, Cell Marque, Rocklin, CA, USA), S100 (Z0331, Dako Corporation, Agilent), vimentin (Vim384, Dako Corporation, Agilent), E-cadherin (EP700Y, Cell Marque, Darmstadt, Germany), pancytokeratin (PCK, AE1/3, Leica Biosystems, Nußloch, Germany), EGFR (E30, Dako Corporation, Agilent), VEGF (VG-1, Santa Cruz Biotechnology, Dallas, TX, USA), SMARCB1 (25/BAF47, BD Biosciences, Franklin Lakes, NJ, USA), https://doi.org/10.3390/cancers18060898 lamin A/C (EP4520-16, Abcam Cambridge Biomedical Campus, Cambridge, UK), PD-1 (315M-95, Cell Marque, Rocklin, CA, USA) and PD-L1 (M3653, Dako Corporation, Agilent). ..

    Article Title: Correlation Between Molecular Genetic Analysis and Nuclear Pleomorphism in Long-Term Recurrent and Metastatic Chordoma
    Article Snippet: .. Immunohistochemical staining was performed on all samples for brachyury ( EPR18113 , Abcam limited, Cambridge, UK), Ki-67 (MIB-1, Dako Corporation, Agilent, Santa Clara, CA, USA), p53 (453M-95, DO7, Cell Marque, Rocklin, CA, USA), S100 (Z0331, Dako Corporation, Agilent), vimentin (Vim384, Dako Corporation, Agilent), E-cadherin (EP700Y, Cell Marque, Darmstadt, Germany), pancytokeratin (PCK, AE1/3, Leica Biosystems, Nußloch, Germany), EGFR (E30, Dako Corporation, Agilent), VEGF (VG-1, Santa Cruz Biotechnology, Dallas, TX, USA), SMARCB1 (25/BAF47, BD Biosciences, Franklin Lakes, NJ, USA), lamin A/C (EP4520-16, Abcam Cambridge Biomedical Campus, Cambridge, UK), PD-1 (315M-95, Cell Marque, Rocklin, CA, USA) and PD-L1 (M3653, Dako Corporation, Agilent). ..

    Analysis:

    Article Title: Immunohistochemical Evaluation of Non-Thermal Plasma-Treated Xenogenic Bone Graft for Enhanced Bone Regeneration in a Rabbit Calvarial Defect Model
    Article Snippet: .. Immunohistochemical analysis was performed using antibodies against proliferating cell nuclear antigen (PCNA; mouse monoclonal, PC10, Santa Cruz Biotechnology, Dallas, TX, USA; 1:200), vascular endothelial growth factor (VEGF; rabbit polyclonal, A-20, Santa Cruz Biotechnology, Dallas, TX, USA; 1:100), platelet endothelial cell adhesion molecule-1 (PECAM-1; goat polyclonal, M-20, Santa Cruz Biotechnology, Dallas, TX, USA; 1:100), collagen I (rabbit monoclonal, ab138492, Abcam, Cambridge, UK; 1:100), collagen III (mouse monoclonal, FH-7A, Abcam, Cambridge, UK; 1:500), alkaline phosphatase (ALP; rabbit monoclonal, ab307726, Abcam, Cambridge, UK; 1:500), bone morphogenetic protein 2 (BMP2; mouse monoclonal, ab6285, Abcam, Cambridge, UK; 1:200), BMP4 (rabbit polyclonal, ab39973, Abcam, Cambridge, UK; 1:500), BMP7 (rabbit polyclonal, ab84684, Abcam, Cambridge, UK; 1:500), osteocalcin (rabbit polyclonal, ab198228, Abcam, Cambridge, UK; 1:200), and osteopontin (mouse monoclonal, ab69498, Abcam, Cambridge, UK; 1:100). ..

    ALP Assay:

    Article Title: Immunohistochemical Evaluation of Non-Thermal Plasma-Treated Xenogenic Bone Graft for Enhanced Bone Regeneration in a Rabbit Calvarial Defect Model
    Article Snippet: .. Immunohistochemical analysis was performed using antibodies against proliferating cell nuclear antigen (PCNA; mouse monoclonal, PC10, Santa Cruz Biotechnology, Dallas, TX, USA; 1:200), vascular endothelial growth factor (VEGF; rabbit polyclonal, A-20, Santa Cruz Biotechnology, Dallas, TX, USA; 1:100), platelet endothelial cell adhesion molecule-1 (PECAM-1; goat polyclonal, M-20, Santa Cruz Biotechnology, Dallas, TX, USA; 1:100), collagen I (rabbit monoclonal, ab138492, Abcam, Cambridge, UK; 1:100), collagen III (mouse monoclonal, FH-7A, Abcam, Cambridge, UK; 1:500), alkaline phosphatase (ALP; rabbit monoclonal, ab307726, Abcam, Cambridge, UK; 1:500), bone morphogenetic protein 2 (BMP2; mouse monoclonal, ab6285, Abcam, Cambridge, UK; 1:200), BMP4 (rabbit polyclonal, ab39973, Abcam, Cambridge, UK; 1:500), BMP7 (rabbit polyclonal, ab84684, Abcam, Cambridge, UK; 1:500), osteocalcin (rabbit polyclonal, ab198228, Abcam, Cambridge, UK; 1:200), and osteopontin (mouse monoclonal, ab69498, Abcam, Cambridge, UK; 1:100). ..



    Similar Products

    95
    TaKaRa slide seq spatial transcriptomics experiment
    AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and <t>transcriptomics</t> in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)
    Slide Seq Spatial Transcriptomics Experiment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slide+seq+spatial+transcriptomics+experiment/Seeker+Spatial+Transcriptomics+Kit/pmc12867689-204-0-8
    Average 95 stars, based on 1 article reviews
    slide seq spatial transcriptomics experiment - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    TaKaRa seeker spatial transcriptomics kit
    AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and <t>transcriptomics</t> in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)
    Seeker Spatial Transcriptomics Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slide+seq+spatial+transcriptomics+experiment/Seeker+Spatial+Transcriptomics+Kit/custom%40sk005%4041326786
    Average 95 stars, based on 1 article reviews
    seeker spatial transcriptomics kit - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and transcriptomics in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection

    doi: 10.1073/pnas.2504742123

    Figure Lengend Snippet: AIR-SPACE enables the mapping of adaptive immune receptor (AIR) clonotypes and transcriptomics in situ. ( A ) Schematic of the experimental design and methodology, including the generation of long-read (LR) and short-read (SR). ( B ) Spatial mapping of cell types across the LN sections at different time points postinfection. (Scale bar, 500 μm.) ( C ) Spatial mapping of AIR clonotypes across the LN sections, with immunoglobulin (IG) clones shown in blue and T cell receptor (TCR) clones shown in red; outlined with germinal center (GC) regions in LNs from D10PI to D21PI. ( D ) Multiplexed RNA FISH staining for T cell marker Trbc2 (green), B cell marker Ms4a1 (red), and DAPI (blue) across all samples on sister sections. (Scale bar 500 μm.)

    Article Snippet: Slide-seq spatial transcriptomics experiment was performed using the Curio Seeker Kit (Curio Bioscience) according to manufacturer instructions.

    Techniques: In Situ, Clone Assay, Staining, Marker